WBC ruling in Hemocytometer

WBC ruling

PRESENTED BY:PRINCESS ALEN AGUILAR

Counting the WBC

• Keep the counting chamber horizontal• Place the hemocytometer on the stage

of microscope• Using the low power (10x) objective,

make ccertain the microscope light is adjusted correctly.

• In proper focus, the white blood cells should look small dark dots

• Scan the four large corner squares marked "W".

• For accurate white counts , there should be an even distribution of cells in all four large squares , with no more than a ten-cell variation between the four cells.

• Beggining w/ the upper left square count all th e WBC per large squares with a pattern of UP->UR->LR->LL then add all the WBC per squares to get the total number of cells.

Four corner squares are meant for WBC counting.Total = 64 small squares

if using a UNOPETTE method?

• ALL NINE LARGE SQUARES OF THE HEMOCYTOMETER GRID ARE USED TO COUNT WBCs

Counting per square

• in counting the cells that touch the outside lines of the outside of the square, count only those that touch the left (or right) and upper (or lower) outside lines.

• in counting the chambers with double lines, disregarding those touch the right (of left) and lower (or upper) outside margin.

• If the chamber has 3 lines , count those cells that touch the middle line of the three

outside lines on two sides and disregard those touching the corresponding lines on the other two sides.

• Consistency is a must

• Count the cells on the opposite side of the counting chamber and record the number of cells counted in these four large squares.

• The total number of cells counted on each side of the counting chamber should agree within 10% of each other . if the count should not agree, the procedure must be repeated.

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HEMATOLOGY LABORATORY