Ways of Studying Cells A. Visualization of Cells SCI 420 Advanced Cell Biology Sept 11, 2002 Any nation that does not honor its heroes will not ndure.” -Abraham Lincoln In honor of those who died 9/11/2001 and those inju n searching the rubble in the aftermath)
Ways of Studying Cells A. Visualization of Cells. BSCI 420 Advanced Cell Biology Sept 11, 2002 “Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln (In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath). - PowerPoint PPT Presentation
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Ways of Studying Cells A. Visualization of Cells
BSCI 420 Advanced Cell Biology Sept 11, 2002
“Any nation that does not honor its heroes will not long endure.” -Abraham Lincoln
(In honor of those who died 9/11/2001 and those injured in searching the rubble in the aftermath)
Resolving Power
Bright-fieldlight microscope
Resolution depends on the Numerical Aperture
Types of LM: A) Bright field B) Phase contrast
Types of LM: C) Diff. Interference Contrast
D) Dark field
Bright field microscopy of stained section of kidney tubules
Bright field vs. Phase contrast
Constructive and destructive interference of light
Fluorescence Microscope
Fluorescence image of a cell in mitosis labeled w 3 tags:Green: fluorescein-labeled anti-tubulin (MTS)Red: anti-centromereproteinBlue: DAPI(DNA)
Indirect Immunocytochemistry
Excitation and Emission wave-lengths
Note emission w-l is always > thanexcitation w-l.So they have lost energy(Energy = 1/wave-length
Immunofluorescence of individual MTs
Images of Conventional (A) vs Confocal (B) Fluorescence
(Drosophila embryo stained w fluorescent probe against actin)
GFT tagged talin in trichrome cells of Arabidopsis
Dynamics of GFP-labeled Cajal bodies in the nucleus