Methods in Enzymology Volume 28 0 Vitamins and Coenzyme s Part J EDITED B Y Donald B . McCormick DEPARTMENT OF BIOCHEMISTR Y EMORY UNIVERSITY ATLANTA, GEORGIA John W . Sutti e DEPARTMENT OF BIOCHEMISTRY UNIVERSITY OF WISCONSI N MADISON, WISCONSIN Conrad Wagne r DEPARTMENT OF BIOCHEMISTRY VANDERBILT UNIVERSITY AND VA MEDICAL CENTER NASHVILLE, TENNESSEE
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Vitamins and Coenzymes - GBVsis of Pyrroloquinoline Quinone TAKESHI KUMAZAWA 150 14. Monoclonal Antibodies Specific to Pyrroloquino- HIROSHI NARITA AND line Quinone EMI MORISHITA 158
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Methods in Enzymology
Volume 280
Vitamins and CoenzymesPart J
EDITED BY
Donald B. McCormickDEPARTMENT OF BIOCHEMISTR Y
EMORY UNIVERSITY
ATLANTA, GEORGIA
John W. SuttieDEPARTMENT OF BIOCHEMISTRY
UNIVERSITY OF WISCONSIN
MADISON, WISCONSIN
Conrad WagnerDEPARTMENT OF BIOCHEMISTRY
VANDERBILT UNIVERSITY AND VA MEDICAL CENTER
NASHVILLE, TENNESSEE
CONTRIBUTORS TO VOLUME 280 .
i x
PREFACE Xii i
VOLUMES IN SERIES x v
Section I. Pyridoxine, Pyridoxamine, Pyridoxal :Analogs and Derivative s
1 . Determination of Vitamin B 6 Vitamers and Metab- HARUHITO TSUGE
3
olites in a Biological Sampl e
2 . High-Performance Liquid Chromatography Deter- HERMANN J. MASCHER
1 2
mination of Total Pyridoxal in Human Plasm a
3 . Determination of 5-Pyridoxic Acid, 5-Pyridoxic J. DENNIS MAHUREN AN DAcid Lactone, and Other Vitamin B 6 Compounds STEPHEN P . COBURN
2 2
by Cation-Exchange High-Performance LiquidChromatograph y
4. Nuclear Magnetic Resonance in Study of Active DAVID E . METZLER
3 0
Sites of Pyridoxal-Dependent Enzyme s
5. Synthesis and Application of Pyridoxal Polyphos- TOSHIO Fuxul ANDphoryl Derivatives as Active-Site Probes for Nu- KATSUYUKI TANIZAWA
4 1
cleotide-Binding Enzyme s
6. Preparation of Vitamin B6-Peptide and Vitamin STANLEY STEIN ANDB 6-Peptide-Oligonucleotide Conjugates
TIANMIN ZHU
5 1
7. Preparation of Nonlabeled, Tritiated, and Deuter- JESSE F . GREGORY III ANDated Pyridoxine 5'-ß-D-Glucoside and Assay of HIDEKO NAKANO
5 8Pyridoxine-5'-ß-D-glucoside Hydrolas e
8. Enzymatic Preparation of Pyridoxine 4'- and 5'-a- YUKIO SUZUKI ,D-Glucosides
YUSUKE Doi ,KEI UCHIDA, ANDHARUHITO TSUGE
6 6
9. Formation of ß-Galactosides of Pyridoxine Using YUKIO SUZUKI AN DSporobolomyces singularis
KEI UCHIDA
71
Section II . Carbonyl Coenzymes: Pyruvyl Enzyme sand Quinoproteins
10 . Phosphatidylserine Decarboxylases : Pyruvoyl-De- WILLIAM DOWHAN
8 1
pendent Enzymes from Bacteria to Mammals
11. Production, Assay, and Occurrence of Pyrrolo- M . MISSET-SMITS ,quinoline Quinone
A. J . J. OLSTHOORN ,A . DEWANTI, ANDJ . A . DUINE
8 9
12. Amine-Oxidizing Quinoproteins
CHRISTA HARTMANN ANDWILLIAM S . MCINTIRE
98
13 . Gas Chromatographic-Mass Spectrometric Analy- OSAMU SUZUKI ANDsis of Pyrroloquinoline Quinone
TAKESHI KUMAZAWA
150
14. Monoclonal Antibodies Specific to Pyrroloquino- HIROSHI NARITA AN Dline Quinone
EMI MORISHITA
15 8
Section III . Nicotinic Acid: Analogs and Coenzymes
15. Determination, Purification, and Characterization ANDREE KLEMM ,
of a-NADH and a-NADPH
THOMAS STEINER ,UWE FLÖTGEN ,GERHARD A. CUMME, ANDANTON HORN
17 1
16. Affinity Labels for NAD(P)-Specific Sites
ROBERTA F . COLMAN
186
17 . Photoaffinity Labeling of NAD'-Linked Enzymes CHARLES MARK ENSOR AN DHSIN-HSIUNG TAI
204
18. Determining NAD Synthesis in Erythrocytes
VANNA MICHELI AN DSILVIA SESTINI
21 1
19 . Tissue NAD as a Biochemical Measure of Niacin ELAINE L . JACOBSON AN DStatus in Humans
MYRON K . JACOBSON
22 1
20 . Radioimmunoassay for Measuring Endogenous RICHARD M . GRAEFF ,Levels of Cyclic ADP-Ribose in Tissues
TIMOTHY F . WALSETH, ANDHON CHEUNG LEE
23 0
21. Purification of Human Nicotinamide-Mononucleo- GIuuo MAGNI ,tide Adenylyltransferase
MONICA EMANUELLI ,ADOLFO AMICI ,NADIA RAFFAELLI, AN DSILVERIO RUGGIERI
24 1
22 . Nicotinamide-Mononucleotide Adenylyltransfer- GIULIO MAGNI ,ases from Yeast and Other Microorganisms