Visual Protein 產品介紹 - Valter Occhienavalterocchiena.com/pdf/visual-protein-product-2015.pdf · 2016. 1. 21. · Sensitivity ComparisonChart ~6pg ~1-3pg ~400fg Visual Protein

Visual Protein

2015

Four Main Sectors

1. Western Blotting

2. Antibody Production

3. Protein Purification

4. Proteomics

Established in 2005, Visual Protein Biotechnology Corporation is dedicated to enhancing biological developments by providing innovative products and services for research use. Our products are divided into four sectors.

Western Blotting

LumiFlashTM Series

BlockPROTM Series

LuminolPenTM Series

LumiFlashTM Series

Western Blotting

LumiFlash Prime LumiFlash Ultima LumiFlash Infinity

Item No. LF01 LF08 LF16

PackageLF01-500/LF08-500/LF16-500: 250mL SolnA + 250mL Soln B

LF01-100/LF08-100/LF16-100: 50mL SolnA + 50mL Soln BLF01-10S/LF08-10S/LF16-10S: 5mL SolnA + 5mL Soln B

Sensitivity ~6 pg ~1-3pg ~400fg

Exposure time 3min (X-ray film) ~1 min (X-ray film) ~1 min (X-ray film)

Shelf life 14 months from date of despatch

Storage temp. 4°C

Shipping temp. 4°C

LumiFlashTM Series is an ECL substrate with high sensitivity and more competitive price than other brands. Depending on the sensitivity, we offerthree classes: LumiFlash Prime, LumiFlash Ultima, and LumiFlash Infinity.

Product information

Sensitivity ComparisonChart

~6pg ~1-3pg ~400fg

Visual Protein LumiFlash Prime LumiFlash Ultima LumiFlash Infinty

MilliporeLuminata Classico Luminata Crescendo Luminata Forte

- - Immobilon

Thermo PiercePierce ECL - Pierce ECL Plus

- SuperSignal Pico SuperSignal Dura

Advansta - WesternBright ECL kit WesternBright Quantum

PerkinElmer Lightning ECL Lightning ECL Plus Lighting ECL Pro

LumiFlashTM Series

Western Blotting

LumiFlashTM Prime Chemiluminescent Substrate

a. High sensitivity–detect protein target to picogram level

b. Antibody saving–less antibody usage with higher quality result on the WB and ELISA application

c. Low background–provide low background on the WB result

d. Economic–offer excellent quality with lower price

The comparison of WB application for LumiFlash Prime, VisGlow and Luminata Crescendo. Hela cell lysate with 1/2 serial dilution from 20 μg was separated by 12.5% SDS-PAGE and probed by anti β-actin. All results were exposed to X-ray film for 1 minute.

Western Blotting

LumiFlashTM Ultima Chemiluminescent Substrate

a. Bettersignal sensitivity–detection of target protein at low-picogram level on WB and ELISA application

b. Antibody saving–diluted lower antibody concentration for WB and ELISA application

c. Long duration–provide stable signal for long exposure

d. Economy–cost less than other ECL substrate with similar sensitivity level

The comparison of WB application for LumiFlash Ultima, VisGlow Plus and Luminata Crescendo. K562 cell lysate with 1/2 serial dilution from 50 μg was separated by 12.5% SDS-PAGE and probed by anti HSP 47. All results were exposed to X-ray film for 3 minutes.

Western Blotting

LumiFlashTM Infinity Chemiluminescent Substrate

a. High sensitivity–detect femtogram of protein target on PVDF or nitrocellulose membrane

b. Antibody saving–use very low antibody concentration on the WB and ELISA application; diluteprimary antibody 5000 to 20,000-fold and secondary antibody 100,000 to 400,000-fold (from 1mg/mL stock)

c. Low background–provide insignificant background and avoid non-specific signals on the WB result

The comparison of WB application for LumiFlash Infinity and LuminataForte. Hela cell lysate with 1/2 serial dilution from 20 µg was separated by 12.5% SDS-PAGE and probed by anti AMPKα1. All results were exposed to X-ray film for 5 minutes.。

Western Blotting

BlockPROTM Series

BlockPRO™ Series is suitable for blocking in Western blot, ELISA and other immunochemical application. We offer twooptions, both protein-based and synthetic-based, to block excess binding site but not cover on the binding protein and therefore increase the signal intensity, present better data result than milk and BSA blocking buffer.

Product information

BlockPROTM BlockingBlockPROTM Protein-Free Blocking

Buffer

Item No. BP01 BF01

Package BP01-1L: 500mL x 2 BF01-1L: 500mL x 2

Sensitivity Single protein Synthetic

Target customer

For those who are looking for replacement of traditional blocking buffer and caring about the budget

For those who pursue highest quality of experiments

Shelf life 12 month after despatch

Storage temp.After opening,

store at 4°C

Shipping temp. Room temperature

Western Blotting

BlockPROTM Blocking Buffer

a. High efficiency : Increase signal intensity

b. High compatibility : Suitable for downstream PBST and TBST wash procedure and also compatible with every immunochemical application

c. Simple : Safe and ready to use

Signal strength comparison of Casein, BSA, milk, and BlockPRO™ Blocking Buffer. Loading 30 μg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000. Membrane: Hyond™ P. Detection : Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-rayfilm.

Membranes detected by SEM. The SEM result showed that BlockPROTM Blocking Buffer only blocking on excess binding site and reveal the position of antigen and therefore will not reduce the signal intensity.

Western Blotting

BlockPROTM Protein-Free Blocking Buffer

a. Protein-free and biotin-free: Suitable for biotin/avidin system

b. Multiple application:suitable for Western blot, dot blot, ELISA, and other immunoassays

c. High quality control: ensure lot-to-lot consistency and reproducible results over time

BlockPRO™ Protein-Free is suitable for various protein antigen detection, such as high molecular weight protein, tACC; phosphoprotein, pAMPK; abundant protein, GAPDH; low molecular weight protein, histone H3.

BlockPROTM Protein-Free Blocking Buffer is better than protein-based blocking buffers (skim milk, BSA and casein) for detection of target protein in Western blotting

Western Blotting

LuminolPenTM Series

LuminolPen™ Series is a perfect tool for chemiluminescent detection in Western blot. With simply writing on the pre-stained marker, the position of the molecular weight can be showed on the image. It could also help you to note the experiment condition or evaluate the efficacy of ECL substrate. We offer two versions of LuminolPen based on their chemiluminesent properties: enzyme-based (LH03) and chemical based (LH604).

Western Blotting

LuminolPenTM , HRP System LuminolPenTM Lite, HRP System

Item No. LH03 LH604

Package

LH03-50: about 1,000 membranesDrawing

LH03-10: about 100 membranesdrawing

LH604-200: about 500 membranesDrawing

LH604-30: about 100 membranesdrawing

Target customer

For those who want to know the predicted molecular weight of target protein in Western blot analysis, but have no budget to buy a gel documentation system.


Storage temp. 4°C

Shipping temp. 4°C

Product information

LuminolPen™, HRP System

a. Unique: the world’s first luminescent color development marker pen

b. Help you to evaluate the efficacy of your ECL substrate

c. Convenient: able to make note and show on the image with the data result

d. Compatible: applicable with most brands of ECL substrate

Labeling molecular weight and note on the membrane with LuminolPen™, HRP System.

Western Blotting

After transferring gel proteins onto the nitrocellulose or PVDF membranes and before the procedure of blocking, use the LuminolPen to annotate the prestained marker and perform the following hybridization procedures.

Annotate the standard ladder on the membrane right after gel transfer

LuminolPen™ Lite, HRP System

Western blot detection of GAPDH (36 kD) and annotation of protein standard using LuminolPenTM Lite.

Western Blotting

a. Economic: less expensive than LuminolPenTM, HRP System

b. Help you to evaluate the efficacy of your ECL substrate

c. Convenient: able to make note and show on the image with the data result

d. Compatible: applicable with most brands of ECL substrate

After the procedure of hybridization, use the LuminolPen Lite to annotate the prestainedmarker and perform the following chemiluminescent detection.

Antibody Production

HybriMoreTM Hybridoma Cloning Factor

ImmunoFastTM Adjuvant


HybriMoreTM Hybridoma Cloning Factor is the first completely defined growth promoting supplement especially for hybridoma cells. It could substantially increase the cloning efficiency of the newly fused hybridoma cells. It also significantly increases thesuccessful rate of monoclonization. The addition of HybriMore will not alter the yield of antibody secretion in hybridoma cells.

Antibody Production

Product information


Item No. HB01-1L

Package 0.05g/bottle

Target customer For antibody manufacturer and researchers

Shelf life -

Storage temp. 4°C

Shipping temp. 4°C

More colony formation More hybridoma cells No more alteration in antibody production

Up to 10 fold increasing numbers of recovered hybridoma clones. The newly PEG fused hybridomacells were plated into a 96-well plate containing FCS media with HybriMore (green bars), FCS media with feeder layer (blue bars), or regular FCS media (white bars). Hybridoma cells were subject to HAT selection 14 days after the cell fusion. Two mouse myeloma fusion partners, NS-1 and SP2/0, were evaluated by four independent fusion experiments with freshly prepared mouse spleens. A significant higher cloning efficiency was observed in the usage of HybriMore.

Antibody Production


Increase the successful rate of monoclonization. Eight clones of hybridoma cells from NS-1 or SP2/0 fusion partners were monoclonized in the media containing FCS media with HybriMore (green bars), FCS media with feeder layer (blue bars), or regular FCS media (white bars). The numbers of viable hybridomacolonies in a well were visually counted under a microscope. A significant higher successful rate of monoclonization was observed in the usage of HybriMore.


Antibody Production

Defined chemicals, no animal source materials, no effect on cell physiology. A clone of hybridoma cells (anti human transferrin, L3B5) was cultured in the media containing FCS media with HybriMore (green bars), FCS media with feeder layer (blue bars), or regular FCS media (white bars) for seven days. The supernatants were harvest and examined by the titer of secreting Ab by ELISA assay. The usage of HybriMore will not alter the yield of secreting Ab in hybridoma cells.


Antibody Production

ImmunoFastTM Aduvant

ImmunoFast™ Adjuvant is a new aqueous emulsify adjuvant which can efficiently elicit significant immunorespond and induce high amount of IgG production in very short time. The aqueous texture makes the antigen mixing process and the adjuvant injection much easier. The specially designed non toxicity formula provides higher survival rate of immunized animals during experiment period.

Product information

ImmunoFastTM Adjuvant

Item No. IF01

PackageIF01-4N: For 4 injections for mouse

IF01-20N: For 20 injections for mouse

Target customer For antibody manufacturer and researchers


Storage temp. Room temp.

Shipping temp. Room temp.

Antibody Production


Antibody Production

ImmunoFastTM can elicit much stronger immuneresponse in animals than CFA method. The comparison of immunoresponse elicited by ImmunoFastTM and CFA (complete Freund Adjuvant) at the 2nd and the 6th week after first immunization. Mice were immunized with 50μg of bovine serum albumin in ImmunoFastTM & CFA . The adjuvant boosted in 2nd, 4th and 6th week. The serum IgG tilters were measured by ELISA.

Less time required for the induction of immunoresponse time. Time required for ImmunoFastTM and CFA/IFA to induce an appreciable immunoresponse. Mice were immunized with 50μg of bovine serum albumin in ImmunoFastTM & CFA / IFA. The adjuvant boosted according to the recommended procedure. Then serum IgG tilters were measured by ELISA.


Antibody Production

ImmunoFastTM elevate the production of IgG. Levels of the elicited immunoglobulin molecules in an ImmunoFastTM boosted animal. Mice were immunized with 50μg of bovine serum albumin in ImmunoFastTM. The adjuvant boosted in 2nd, 4th and 6th week. Then IgG and IgM tilters were measured by ELISA.

Protein Purification

ExtractPROTM Protein Extraction Reagent

FracPROTM Protein Fractionation Kit

PhosPROTM Phosphoprotein Enrichment Kit

PhosPROTM Phosphoprotein Purification Kit


Shorten experiment time with lower cost

Protease inhibitor and freeze/thaw cycle are not required

Compatible for most bio-samples, including plant, animal, tissue, and microorganism protein extraction

Effectively maintain protein condition during long term preservation with only ExtractPRO™ reagent


Product information


Item No. EP05-30

Package 30mL solution

Target customer For protein research


Storage temp. 4°C

Shipping temp. 4°C

Protein extract efficiency with different sample source. Cell line (K562), animal tissue (pig liver), fungi (PE1), and bacteria (E. coli) extracted by freeze and thaw or ExtractPRO™; 12.5% SDS-PAGE stained by CBR method. ExtractPRO™ extract high amount of total protein from different sample than traditional freeze and thaw method.



Comparison of sample preparatrion by the ExtractPROTM Protein Extraction Reagent with freeze and thaw method. HepaG2 cell is prepared by traditional sample preparation (freeze & thaw) and ExtractPROTM Protein Extraction Reagent. The 2D-PAGE treated by ExtractPRO has lower background than by traditional sample preparation.

Total protein extract amount after storage in ExtractPRO. Cell line SP-2 preserved in Extract-PRO without protease inhibitor for 0, 14, and 28 days. Protein quantified by bradford method. The protein portion in sample is well preserved during long-term storage in ExtractPRO,



• Online Video

http://www.visualprotein.com/vispro/index.php/protein-purification/extractpro-protein-extraction-reagent


Product information


Item No. FP01-5 FP01-50

Package

10ml Cytosol buffer +10ml Membrane/Organelle buffer +

7.5ml Nuclear buffer +1.5ml Cytoskeletal buffer

100ml Cytosol buffer +100ml Membrane/Organelle buffer +

75ml Nuclear buffer +15ml Cytoskeletal buffer

Target customer

For protein fractionation experiment


Storage temp.

4°C

Shipping temp.

4°C


FracPROTM Protein Fractionation Kit is a simple, reproducible and ultracentrifuge-independent method to separate four subcellular protein fractions, including nuclear, cytosol, membrane/organelle, and cytoskeletal fractions, in a detergent-based microcentrifuge procedure. All of the separated fractions from FracPROTM Protein Fractionation Kit can directly be used in SDS-PAGE, enzyme analyses, Western blotting, gel mobility shift assays, and other procedures.



The Protein fractions of Human leukemia cell line (K562) were separated by FracPRO™ Protein Fractionation Kit and presented by Western blot.

Variable sample application including cultured cells and mammalian tissues

Separate and collect four subcellular protein fractions in short time

Isolate high quality protein fractions with easy procedure



Item No. PP03-2C PP03-6C PP03-5E

Package

2 pre-packed column +2ml Lysis buffer +

250ml System buffer +100ml Elution buffer+100ml Stringent buffer

stock

6 pre-packed column +2ml Lysis buffer +


stock

3 ml Resin +2ml Lysis buffer +


stock

Target customer For phosphoprotein research

Shelf life 6 months after despatch

Storage/shipping temp.

4°C

Product information

PhosPROTM Phosphoprotein Enrichment Kit is based on IMAC (immobilized metal affinity chromatography), which can isolate the most amount of phosphoprotein from different biological materials, such as animal, plant, and microorganism.


Purification efficacy of PhosPRO™ Phosphoprotein Enrichment Kit (A) and Brand Q kit (B). Protein standard markers contain glycogen phosphorylase b (GP), bovine serum albumin (BSA), ovalbumin (OVA), carbonic anhydrase (CA), trypsin inhibitor (TI), and lactalbumin (LAC) were used for evaluation. Ovalbumin was the only phosphoprotein in the test material. F: flow through; E: eluent; VisPRO™ 5 minute Protein Stain Kit (VisPRO™) and Pro-Q Diamond stain (Pro-Q, from Invitrogen) were used for developing total proteins or phosphoproteins.

Optimize suitable chromatographic condition with PhosPRO™ Phosphoprotein Enrichment Kit.

A) B)




Product information


Item No. PP05-2C PP05-6C PP05-5E

Package

Pre-packed column package2 pre-packed column +

2ml Lysis buffer +250ml System buffer +100ml Elution buffer +

100ml stringent buffer stock

Pre-packed column package6 pre-packed column +

2ml Lysis buffer +250ml System buffer +100ml Elution buffer +


Evaluation package 3ml resin +2ml Lysis buffer +

250ml System buffer +100ml Elution buffer +


Target customer

For phosphoprotein research

Shelf life 6 months after despatch


4°C


PhosPROTM Phosphoprotein Purification Kit is based on IMAC (immobilized metal affinity chromatography), which can isolate the phosphoprotein and ensure its purity from different biological materials, such as animal, plant, and microorganism.

Purification efficacy from (A) PhosPRO® Phosphoprotein Purification Kit, (B) Brand Q kit. Protein standard markers containing glycogen phosphorylase b (GP), bovine serum albumin (BSA), ovalbumin (OVA), carbonic anhydrase (CA), trypsin inhibitor (TI) and lactalbumin (LAC) were used for evaluation. Ovalbumin was the only phosphoprotein in the test material. F: flow through; E: eluent. VisPRO® 5 minute Protein Stain Kit (VisPRO) and Pro-Q Diamond stain (Pro-Q, from Invitrogen) were used for developing total proteins or phosphoproteins.



Proteomics

VisPROTM 5 Minutes Protein Stain Kit

IEF Optimizer


Proteomics

VisPROTM 5 minutes Protein Stain Kit

VisPROTM 5 minutes Protein Stain Kit (5X)

VisPROTM 5 minutes Protein Stain Kit (10X)

Item No. VP01 VP05 VP10

Package

VP01-125: 125ml soln.1 + 125ml soln.2

VP01-500: 500ml soln.1 + 500ml soln.2

VP05-125: 5X 125ml soln.1 + 125ml soln.2

VP05-500: 5X 500ml soln.1 + 500ml soln.2

VP10-1L: 10X 500ml soln.1 + 500ml soln.2

Target customer For protein research, especially the downstream procedure



Room temp.

Product information

VisPROTM 5 minutes Protein Stain Kit is a reverse staining method, which is a modified version of the imidazole- zinc reverse stain, designed for convenience and superior performance. The stained target protein can be eluted directly and apply downstream experiments, such as Western blotting, LCMSMS analysis. The staining solution is also highly stable, non-biohazard to operator and environment.


Proteomics

VisPRO applies the principle of zinc stain. The formation of zinc-imidazole complex on polyacrylamide turns the gel to white, while the protein area remain transparent.

Most protein gel staining methods apply the principles of positive staining, such as CBR、silver stain、SYBRO ruby.


Proteomics

Unlike conventional positive staining methods, reverse staining can be completed in a short time because the target of staining is the gel rather than the proteins. Therefore, reverse staining methods allow researchers to obtain the results promptly.


Proteomics

VisPROTM 5 Minutes Protein Stain kit perfectly matches to current proteomic research. More protein spots can befoundon the two-dimensional electrophoresis (2-DE) gel developed with the VisPROTM 5 Minutes Protein Stain Kit (right) than the one developed with other high sensitivity protein staining methods, such as silver stain (left).


Proteomics

MALDI-TOF analysis of protein spots visualized with the VisPROTM 5 minutes Protein Stain Kit. The gel spot of rabbit phosphorylase b (10 ng) was cut and subjected to mass spectrometry. Twenty-two out of fifty signals were categorized as tryptic fragments of phosphorylase b.

IEF Optimizer

IEF Optimizer is a novel and convenient instrument which can measure the salt content of the two-dimensional electrophoresis (2-DE) samples. In combined with IEF salt meter and the online accessible voltage-hour calculator, IEF Optimizer can provide you a suggested total voltage hour for IEF process, avoiding underfocusing and overfocusing of proteins and not only give you reproducible data but also accuracy.

Proteomics

IEF Optimizer

Item No. IOR01

DiscriptionIEF Salt Meter + Voltage-Hour Calculator (Online Software)

registration card

Targetcustomer

2D Gel Electrophoresis user

Product information

IEF Optimizer

Proteomics

Providing the accurate salt content measurement of 2-DE samples and optimal IEF total voltage hour for 2-DE samples

Increasing the reproducibility of 2-DE result

Reducing underfocusing and overfocusing of 2-DE experiments, particularly analyzing precious clinical samples during DIGE experiments

Online video

http://www.visualprotein.com/vispro/index.php/proteomics/ief-optimizer

IEF Optimizer

Proteomics

Optimum parameters provided by IEF Optimizer show better results than the suggested protocol

Sample: pig liver1-D electrophoresis is from pH 3-10. 2-DE using 12.5% SDS-PAGE. (LEFT) Results obtained according to the manufacturers’ instruction handbook. (RIGHT) Result obtained according to the IEF Optimizer parameter.

If you have any questions about the product, please contact at [email protected]

Contact us

Visual Protein 產品介紹 - Valter Occhienavalterocchiena.com/pdf/visual-protein-product-2015.pdf · 2016. 1. 21. · Sensitivity ComparisonChart ~6pg ~1-3pg ~400fg Visual Protein

Documents