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Dispatch Date : 20-Nov-2017 Page 1 Viral gastroenteritis Distribution : 4196 Laboratory : Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG © Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018 Your Score Intended Result Your Report Specimen 4119 Adenovirus negative Adenovirus negative 2 Norovirus negative Norovirus negative 2 Rotavirus negative Rotavirus negative 2 Specimen 4120 Adenovirus negative Adenovirus negative 2 Norovirus negative Norovirus negative 2 Rotavirus positive Rotavirus positive 2 Specimen 4121 Adenovirus negative Adenovirus negative 2 Norovirus positive Norovirus positive 2 Rotavirus negative Rotavirus negative 2 Specimen 4122 Adenovirus negative Adenovirus negative 2 Norovirus positive Norovirus positive 2 Rotavirus negative Rotavirus negative 2 Cumulative score information Total number of specimens sent to you for UK NEQAS for Viral gastroenteritis over the last 2 distributions is 8 For these distributions specimen numbers 3631 3632 3633 3634 4119 4120 4121 4122 have been analysed and scored. Number of reports analysed 2 Number of specimens reported as not examined (not scored) 0 Number of specimens received too late for analysis (not scored) 0 Number of specimens for which no report was received (not scored) 0 Your cumulative score for these specimens was 48 out of a possible total of 48 The mean score calculated from the reports returned by UK laboratories was 47.31 (with a standard error of 1.88) Performance rating Your performance rating for UK NEQAS for Viral gastroenteritis (i.e. the number of standard errors by which your cumulative score lies above or below the mean) for UK laboratories is 0.37. A performance rating of more than 1.96 standard errors below the mean indicates possible poor performance. Please note your performance rating may alter if other participants' results are amended. No score penalty is incurred for non return of reports. However non return of results may be used as a measure of poor performance. Comments: Overall performance for this distribution was excellent with 98.7% of participants reporting correct results for Adenovirus, 98.7% for Norovirus and 97.3% for Rotavirus. Please note that these figures are based on the overall report submitted by each participant. The top graph on each page displays results reported from laboratories in your country, if ten or more participate (shaded area), followed by results reported by all participants. The figures in the histograms and those in the overall results tables may differ (1) due to exclusion of kits displayed in the histograms resulting in apparently lower numbers of data sets in the histograms or (2) due to participants using more than one kit resulting in higher numbers of data sets in the histograms. Turn around time: The time taken to report your results was 0 - days. This information is provided for your own use and does not form part of your performance assessment. Acknowledgements: We would like to thank NHS Tayside, Dundee, for providing clinical material and PHE Cambridge for pre-distribution testing. Enquiries: Pre-distribution test results are available should you experience a technical failure and wish to discuss the results. Written enquiries about this distribution should be addressed to Elli Elenoglou at the email address below. For repeat specimens please order using the web form or e-mail [email protected] stating your laboratory identification number, the distribution name, distribution number and specimen numbers. Report authorised by: Dr Sanjiv Rughooputh Director Performance Rating Distribution Your performance rating over the past 4 distributions Your current performance rating is 0.37 MICPERF2 3782 3825 4007 4050 4196 -4 -3 -2 -1 0 1 2 3 4 All UK Total score Distribution Total score you achieved for the last 4 distributions Your total score for this distribution is 24 MICPERF2X 3782 3825 4007 4050 4196 0 4 8 12 16 20 24 28 of 13
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Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dec 31, 2020

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Page 1: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 1

Viral gastroenteritis

Distribution : 4196

Laboratory :

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

Your Score Intended Result Your Report

Specimen 4119 Adenovirus negative Adenovirus negative 2 Norovirus negative Norovirus negative 2 Rotavirus negative Rotavirus negative 2

Specimen 4120 Adenovirus negative Adenovirus negative 2 Norovirus negative Norovirus negative 2 Rotavirus positive Rotavirus positive 2

Specimen 4121 Adenovirus negative Adenovirus negative 2 Norovirus positive Norovirus positive 2 Rotavirus negative Rotavirus negative 2

Specimen 4122 Adenovirus negative Adenovirus negative 2 Norovirus positive Norovirus positive 2 Rotavirus negative Rotavirus negative 2

Cumulative score information Total number of specimens sent to you for UK NEQAS for Viral gastroenteritis over the last 2 distributions is 8 For these distributions specimen numbers 3631 3632 3633 3634 4119 4120 4121 4122 have been analysed and scored. Number of reports analysed 2 Number of specimens reported as not examined (not scored) 0 Number of specimens received too late for analysis (not scored) 0 Number of specimens for which no report was received (not scored) 0 Your cumulative score for these specimens was 48 out of a possible total of 48 The mean score calculated from the reports returned by UK laboratories was 47.31 (with a standard error of 1.88) Performance rating Your performance rating for UK NEQAS for Viral gastroenteritis (i.e. the number of standard errors by which your cumulative score lies above or below the mean) for UK laboratories is 0.37. A performance rating of more than 1.96 standard errors below the mean indicates possible poor performance. Please note your performance rating may alter if other participants' results are amended. No score penalty is incurred for non return of reports. However non return of results may be used as a measure of poor performance.

Comments: Overall performance for this distribution was excellent with 98.7% of participants reporting correct results for Adenovirus, 98.7% for Norovirus and 97.3% for Rotavirus. Please note that these figures are based on the overall report submitted by each participant. The top graph on each page displays results reported from laboratories in your country, if ten or more participate (shaded area), followed by results reported by all participants. The figures in the histograms and those in the overall results tables may differ (1) due to exclusion of kits displayed in the histograms resulting in apparently lower numbers of data sets in the histograms or (2) due to participants using more than one kit resulting in higher numbers of data sets in the histograms. Turn around time: The time taken to report your results was 0 - days. This information is provided for your own use and does not form part of your performance assessment. Acknowledgements: We would like to thank NHS Tayside, Dundee, for providing clinical material and PHE Cambridge for pre-distribution testing. Enquiries: Pre-distribution test results are available should you experience a technical failure and wish to discuss the results. Written enquiries about this distribution should be addressed to Elli Elenoglou at the email address below. For repeat specimens please order using the web form or e-mail [email protected] stating your laboratory identification number, the distribution name, distribution number and specimen numbers. Report authorised by: Dr Sanjiv Rughooputh Director

Per

form

ance

Rat

ing

Distribution

Your performance rating over the past 4 distributionsYour current performance rating is 0.37

MICPERF2

3782 3825 4007 4050 4196-4-3-2-101234

All

UK

Tot

al s

core

Distribution

Total score you achieved for the last 4 distributionsYour total score for this distribution is 24

MICPERF2X

3782 3825 4007 4050 4196048

1216202428

of 13

Page 2: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 2

Viral gastroenteritis

Distribution : 4196

Laboratory :

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4119 Clinical details: 3 year-old with diarrhoea, family outbreak. Intended result: Adenovirus negative

Adenovirus positiveAdenovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(100)1(0.0)

53

053

(0.9)(99.1)

____114

____113

-12

Specimen : 4119 Adenovirus nucleic acid detection

UK (%) All (%)

2 (14.3) 2 (7.4)FTD0 (0.0) 1 (3.7)Gerbion0 (0.0) 1 (3.7)Ridagene1 (7.1) 1 (3.7)bioMerieux : R-gene2 (14.3) 2 (7.4)AusDiag: Easy-Plex2 (14.3) 2 (7.4)Real-Time Single target2 (14.3) 2 (7.4)Unspecified0 (0.0) 3 (11.1)Seegene: Allplex GI3 (21.4) 4 (14.8)Real-Time Multiplex0 (0.0) 4 (14.8)In house2 (14.3) 5 (18.5)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Adenovirus negative Equivocal Adenovirus positive Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus negative

27

0

Overall Results

------------------------------------------------------------

00

100% Correct14

------------------------------------------------------------

100

UK

270

0Adenovirus positive

14

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4119 Adenovirus antigen detection

UK (%) All (%)

2 (5.1) 2 (2.3)Remel : ProSpecT0 (0.0) 2 (2.3)Meridian: Rapid Strip 0 (0.0) 3 (3.4)Ridascreen1 (2.6) 3 (3.4)SD Bioline1 (2.6) 3 (3.4)bioMerieux : Vikia0 (0.0) 4 (4.5)DiaSorin : Liaison5 (12.8) 5 (5.7)Pro-Lab: Proflow1 (2.6) 6 (6.8)Unspecified3 (7.7) 10 (11.4)bioMerieux : bioNexia

10 (25.6) 11 (12.5)Biokit : Biorapid4 (10.3) 15 (17.0)Ridaquick8 (20.5) 16 (18.2)Coris Bioconcept

Number of reports0 4 8 12 16 20

Adenovirus positive Equivocal Adenovirus negative Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus positive

88

87

Overall Results

------------------------------------------------------------

390

98.9% Correct39

------------------------------------------------------------

100

UK

10

0Adenovirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 3: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 3

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4119 Clinical details: 3 year-old with diarrhoea, family outbreak. Intended result: Norovirus negative

Norovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

131(100)78____

(100)

78 131____

2

Specimen : 4119 Norovirus nucleic acid detection

UK (%) All (%)

4 (8.9) 4 (5.3)FTD1 (2.2) 1 (1.3)Real-Time Single target2 (4.4) 2 (2.7)AusDiag: Easy-Plex1 (2.2) 2 (2.7)Serosep: EntericBio0 (0.0) 3 (4.0)Seegene: Allplex GI3 (6.7) 3 (4.0)Cepheid: Smart2 (4.4) 4 (5.3)bioMerieux: FilmArray4 (8.9) 5 (6.7)Rida Gene3 (6.7) 5 (6.7)Real-Time Multiplex3 (6.7) 5 (6.7)In house2 (4.4) 6 (8.0)Unspecified

20 (44.4) 33 (44.0)Cepheid: GeneXpert

Number of reports0 8 16 24 32 40

Norovirus positive Norovirus negative

Your Report : Norovirus negative

Norovirus negative

100

Norovirus positive

45

Overall Results

75Total

75

------------------------------------------------------------UK

045

100

------------------------------------------------------------

0

All

% Correct

Score

Your Score : 2

Specimen : 4119 Norovirus antigen detection

UK (%) All (%)

2 (8.3) 6 (12.5)Rida Screen0 (0.0) 1 (2.1)CerTest0 (0.0) 1 (2.1)Nadal 1 (4.2) 1 (2.1)Meridian: Rapid Strip 1 (4.2) 2 (4.2)SD Bioline4 (16.7) 4 (8.3)Pro-Lab: Proflow3 (12.5) 6 (12.5)Unspecified3 (12.5) 8 (16.7)ImmunoCard

10 (41.7) 19 (39.6)Rida Quick

Number of reports0 4 8 12 16 20

Norovirus positive Equivocal Norovirus negative

Your Report : Norovirus negative

Norovirus equivocal

24

Norovirus positive

48

Overall Results

48% Correct

240

100

------------------------------------------------------------UK

00

Total

Norovirus negative

0

All

100

------------------------------------------------------------

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 4: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 4

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4119 Clinical details: 3 year-old with diarrhoea, family outbreak. Intended result: Rotavirus negative

Rotavirus negative

Number of reports0 40 80 120 160 200

UK(%) All(%) Score

150(100)86____

(100)

86 150____

2

Specimen : 4119 Rotavirus nucleic acid detection

UK (%) All (%)

2 (20.0) 2 (8.7)FTD0 (0.0) 1 (4.3)Gerbion0 (0.0) 1 (4.3)TIB MOLBIOL: LightMix0 (0.0) 1 (4.3)Rida Gene2 (20.0) 2 (8.7)AusDiag: Easy-Plex0 (0.0) 2 (8.7)In house1 (10.0) 2 (8.7)Unspecified0 (0.0) 3 (13.0)Seegene: Allplex GI3 (30.0) 4 (17.4)Real-Time Multiplex2 (20.0) 5 (21.7)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus negative

Rotavirus equivocal

0

Rotavirus positive

23

23

Overall Results

------------------------------------------------------------

100

100% Correct10

------------------------------------------------------------

100

UK

00

0Rotavirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4119 Rotavirus antigen detection

UK (%) All (%)

5 (7.5) 7 (5.9)Remel : ProSpecT1 (1.5) 3 (2.5)bioMerieux : Vikia2 (3.0) 4 (3.4)SD Bioline4 (6.0) 4 (3.4)Meridian: Premier0 (0.0) 4 (3.4)DiaSorin : Liaison7 (10.4) 7 (5.9)Pro-Lab: Proflow2 (3.0) 7 (5.9)Unspecified2 (3.0) 9 (7.6)bioMerieux : bioNexia

10 (14.9) 11 (9.3)Biokit : Biorapid13 (19.4) 14 (11.9)ImmunoCard

5 (7.5) 16 (13.6)Rida Quick10 (14.9) 18 (15.3)Coris Bioconcept

Number of reports0 4 8 12 16 20

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus negative

Rotavirus equivocal

0

Rotavirus positive

118

118

Overall Results

------------------------------------------------------------

670

100% Correct67

------------------------------------------------------------

100

UK

00

0Rotavirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 5: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 5

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4120 Clinical details: 2 year-old with diarrhoea, family outbreak. Intended result: Adenovirus negative

Adenovirus positiveAdenovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(96.2)4(3.8)

53

251

(3.5)(96.5)

____114

____110

-12

Specimen : 4120 Adenovirus nucleic acid detection

UK (%) All (%)

2 (14.3) 2 (7.1)FTD0 (0.0) 1 (3.6)Gerbion0 (0.0) 1 (3.6)TIB MOLBIOL: LightMix0 (0.0) 1 (3.6)Ridagene1 (7.1) 1 (3.6)bioMerieux : R-gene2 (14.3) 2 (7.1)AusDiag: Easy-Plex2 (14.3) 2 (7.1)Real-Time Single target2 (14.3) 2 (7.1)Unspecified0 (0.0) 3 (10.7)Seegene: Allplex GI3 (21.4) 4 (14.3)Real-Time Multiplex0 (0.0) 4 (14.3)In house2 (14.3) 5 (17.9)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Adenovirus negative Equivocal Adenovirus positive Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus negative

28

3

Overall Results

------------------------------------------------------------

10

89.3% Correct14

------------------------------------------------------------

92.9

UK

250

0Adenovirus positive

13

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4120 Adenovirus antigen detection

UK (%) All (%)

1 (2.6) 1 (1.1)Remel : ProSpecT0 (0.0) 2 (2.3)Meridian: Rapid Strip 0 (0.0) 3 (3.4)Ridascreen1 (2.6) 3 (3.4)SD Bioline1 (2.6) 3 (3.4)bioMerieux : Vikia0 (0.0) 4 (4.6)DiaSorin : Liaison5 (13.2) 5 (5.7)Pro-Lab: Proflow1 (2.6) 6 (6.9)Unspecified3 (7.9) 10 (11.5)bioMerieux : bioNexia

10 (26.3) 11 (12.6)Biokit : Biorapid4 (10.5) 15 (17.2)Ridaquick8 (21.1) 16 (18.4)Coris Bioconcept

Number of reports0 4 8 12 16 20

Adenovirus positive Equivocal Adenovirus negative Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus positive

87

86

Overall Results

------------------------------------------------------------

370

98.9% Correct38

------------------------------------------------------------

97.4

UK

10

0Adenovirus negative

1

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 6: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 6

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4120 Clinical details: 2 year-old with diarrhoea, family outbreak. Intended result: Norovirus negative

Norovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

132(100)79____

(100)

79 132____

2

Specimen : 4120 Norovirus nucleic acid detection

UK (%) All (%)

4 (8.5) 4 (5.3)FTD2 (4.3) 2 (2.6)AusDiag: Easy-Plex1 (2.1) 2 (2.6)Serosep: EntericBio2 (4.3) 2 (2.6)BD MAX0 (0.0) 3 (3.9)Seegene: Allplex GI3 (6.4) 3 (3.9)Cepheid: Smart3 (6.4) 4 (5.3)Real-Time Multiplex2 (4.3) 4 (5.3)bioMerieux: FilmArray4 (8.5) 5 (6.6)Rida Gene3 (6.4) 5 (6.6)In house2 (4.3) 6 (7.9)Unspecified

20 (42.6) 33 (43.4)Cepheid: GeneXpert

Number of reports0 8 16 24 32 40

Norovirus positive Norovirus negative

Your Report : Norovirus negative

Norovirus negative

100

Norovirus positive

47

Overall Results

76Total

76

------------------------------------------------------------UK

047

100

------------------------------------------------------------

0

All

% Correct

Score

Your Score : 2

Specimen : 4120 Norovirus antigen detection

UK (%) All (%)

2 (9.1) 6 (13.0)Rida Screen0 (0.0) 1 (2.2)CerTest0 (0.0) 1 (2.2)Nadal 1 (4.5) 1 (2.2)Meridian: Rapid Strip 1 (4.5) 2 (4.3)SD Bioline4 (18.2) 4 (8.7)Pro-Lab: Proflow1 (4.5) 4 (8.7)Unspecified3 (13.6) 8 (17.4)ImmunoCard

10 (45.5) 19 (41.3)Rida Quick

Number of reports0 4 8 12 16 20

Norovirus positive Equivocal Norovirus negative

Your Report : Norovirus negative

Norovirus equivocal

22

Norovirus positive

46

Overall Results

46% Correct

220

100

------------------------------------------------------------UK

00

Total

Norovirus negative

0

All

100

------------------------------------------------------------

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 7: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 7

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4120 Clinical details: 2 year-old with diarrhoea, family outbreak. Intended result: Rotavirus positive

Rotavirus positiveRotavirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(5.9)136(94.1)

85

805

(91.3)(8.7)

____149

____13

2-1

Specimen : 4120 Rotavirus nucleic acid detection

UK (%) All (%)

2 (16.7) 2 (8.0)FTD0 (0.0) 1 (4.0)Gerbion0 (0.0) 1 (4.0)TIB MOLBIOL: LightMix0 (0.0) 1 (4.0)Rida Gene1 (8.3) 1 (4.0)BD MAX2 (16.7) 2 (8.0)AusDiag: Easy-Plex1 (8.3) 2 (8.0)Unspecified0 (0.0) 3 (12.0)Seegene: Allplex GI1 (8.3) 3 (12.0)In house3 (25.0) 4 (16.0)Real-Time Multiplex2 (16.7) 5 (20.0)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus positive

Rotavirus equivocal

0

Rotavirus positive

25

1

Overall Results

------------------------------------------------------------

00

96.0% Correct12

------------------------------------------------------------

100

UK

240

0Rotavirus negative

12

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4120 Rotavirus antigen detection

UK (%) All (%)

5 (7.4) 7 (5.9)Remel : ProSpecT1 (1.5) 3 (2.5)bioMerieux : Vikia2 (2.9) 4 (3.4)SD Bioline4 (5.9) 4 (3.4)Meridian: Premier0 (0.0) 4 (3.4)DiaSorin : Liaison7 (10.3) 7 (5.9)Pro-Lab: Proflow2 (2.9) 7 (5.9)Unspecified2 (2.9) 9 (7.6)bioMerieux : bioNexia

10 (14.7) 11 (9.2)Biokit : Biorapid14 (20.6) 15 (12.6)ImmunoCard

5 (7.4) 16 (13.4)Rida Quick10 (14.7) 18 (15.1)Coris Bioconcept

Number of reports0 4 8 12 16 20

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus positive

Rotavirus equivocal

0

Rotavirus positive

119

9

Overall Results

------------------------------------------------------------

30

92.4% Correct68

------------------------------------------------------------

95.6

UK

1100

0Rotavirus negative

65

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 8: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 8

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4121 Clinical details: 4 year-old with diarrhoea and vomiting. Intended result: Adenovirus negative

Adenovirus equivocalAdenovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(98.1)1(1.9)

53

152

(0.9)(99.1)

____115

____114

12

Specimen : 4121 Adenovirus nucleic acid detection

UK (%) All (%)

2 (16.7) 2 (7.7)FTD0 (0.0) 1 (3.8)Gerbion0 (0.0) 1 (3.8)TIB MOLBIOL: LightMix0 (0.0) 1 (3.8)Ridagene1 (8.3) 1 (3.8)bioMerieux : R-gene1 (8.3) 1 (3.8)Unspecified2 (16.7) 2 (7.7)AusDiag: Easy-Plex2 (16.7) 2 (7.7)Real-Time Single target0 (0.0) 3 (11.5)Seegene: Allplex GI2 (16.7) 3 (11.5)Real-Time Multiplex0 (0.0) 4 (15.4)In house2 (16.7) 5 (19.2)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Adenovirus negative Equivocal Adenovirus positive Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus negative

26

0

Overall Results

------------------------------------------------------------

00

100% Correct12

------------------------------------------------------------

100

UK

260

0Adenovirus positive

12

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4121 Adenovirus antigen detection

UK (%) All (%)

2 (5.1) 2 (2.3)Remel : ProSpecT0 (0.0) 2 (2.3)Meridian: Rapid Strip 0 (0.0) 3 (3.4)Ridascreen1 (2.6) 3 (3.4)SD Bioline1 (2.6) 3 (3.4)bioMerieux : Vikia0 (0.0) 4 (4.5)DiaSorin : Liaison5 (12.8) 5 (5.7)Pro-Lab: Proflow1 (2.6) 6 (6.8)Unspecified3 (7.7) 10 (11.4)bioMerieux : bioNexia

10 (25.6) 11 (12.5)Biokit : Biorapid4 (10.3) 15 (17.0)Ridaquick8 (20.5) 16 (18.2)Coris Bioconcept

Number of reports0 4 8 12 16 20

Adenovirus positive Equivocal Adenovirus negative Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus positive

88

88

Overall Results

------------------------------------------------------------

390

100% Correct39

------------------------------------------------------------

100

UK

00

0Adenovirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

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Page 9: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 9

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4121 Clinical details: 4 year-old with diarrhoea and vomiting. Intended result: Norovirus positive

Norovirus positiveNorovirus equivocal

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(1.3)131(98.7)

79

781

(99.2)(0.8)

____132

____1

21

Specimen : 4121 Norovirus nucleic acid detection

UK (%) All (%)

3 (10.0) 3 (6.4)FTD1 (3.3) 1 (2.1)AusDiag: Easy-Plex0 (0.0) 1 (2.1)Gerbion0 (0.0) 1 (2.1)Seegene: Allplex GI2 (6.7) 2 (4.3)Cepheid: Smart3 (10.0) 3 (6.4)Rida Gene1 (3.3) 3 (6.4)Real-Time Multiplex3 (10.0) 4 (8.5)In house2 (6.7) 4 (8.5)bioMerieux: FilmArray2 (6.7) 4 (8.5)Unspecified

13 (43.3) 21 (44.7)Cepheid: GeneXpert

Number of reports0 6 12 18 24 30

Norovirus positive Norovirus negative

Your Report : Norovirus positive

Norovirus negative

100

Norovirus positive

30

Overall Results

47Total

0

------------------------------------------------------------UK

470

100

------------------------------------------------------------

30

All

% Correct

Score

Your Score : 2

Specimen : 4121 Norovirus antigen detection

UK (%) All (%)

2 (8.7) 6 (13.3)Rida Screen0 (0.0) 1 (2.2)Nadal 1 (4.3) 1 (2.2)Meridian: Rapid Strip 1 (4.3) 2 (4.4)SD Bioline1 (4.3) 3 (6.7)Unspecified4 (17.4) 4 (8.9)Pro-Lab: Proflow3 (13.0) 8 (17.8)ImmunoCard

11 (47.8) 20 (44.4)Rida Quick

Number of reports0 6 12 18 24 30

Norovirus positive Equivocal Norovirus negative

Your Report : Norovirus positive

Norovirus equivocal

23

Norovirus positive

0

Overall Results

46% Correct

00

100

------------------------------------------------------------UK

460

Total

Norovirus negative

23

All

100

------------------------------------------------------------

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 10: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 10

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4121 Clinical details: 4 year-old with diarrhoea and vomiting. Intended result: Rotavirus negative

Rotavirus positiveRotavirus equivocalRotavirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(1.2)

150

1(0.0)

____

0

2(98.8)1

(0.7)(0.7)

85____

(98.7)1481

-1

86

1

Specimen : 4121 Rotavirus nucleic acid detection

UK (%) All (%)

2 (20.0) 2 (8.7)FTD0 (0.0) 1 (4.3)Gerbion0 (0.0) 1 (4.3)TIB MOLBIOL: LightMix0 (0.0) 1 (4.3)Rida Gene1 (10.0) 1 (4.3)BD MAX2 (20.0) 2 (8.7)AusDiag: Easy-Plex0 (0.0) 2 (8.7)In house1 (10.0) 2 (8.7)Unspecified0 (0.0) 3 (13.0)Seegene: Allplex GI2 (20.0) 3 (13.0)Real-Time Multiplex2 (20.0) 5 (21.7)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus negative

Rotavirus equivocal

0

Rotavirus positive

23

23

Overall Results

------------------------------------------------------------

100

100% Correct10

------------------------------------------------------------

100

UK

00

0Rotavirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4121 Rotavirus antigen detection

UK (%) All (%)

5 (7.5) 7 (6.0)Remel : ProSpecT1 (1.5) 3 (2.6)bioMerieux : Vikia2 (3.0) 4 (3.4)SD Bioline4 (6.0) 4 (3.4)Meridian: Premier0 (0.0) 4 (3.4)DiaSorin : Liaison7 (10.4) 7 (6.0)Pro-Lab: Proflow2 (3.0) 7 (6.0)Unspecified2 (3.0) 9 (7.7)bioMerieux : bioNexia

10 (14.9) 11 (9.4)Biokit : Biorapid13 (19.4) 14 (12.0)ImmunoCard

5 (7.5) 16 (13.7)Rida Quick10 (14.9) 17 (14.5)Coris Bioconcept

Number of reports0 4 8 12 16 20

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus negative

Rotavirus equivocal

0

Rotavirus positive

117

117

Overall Results

------------------------------------------------------------

670

100% Correct67

------------------------------------------------------------

100

UK

00

0Rotavirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

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Page 11: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 11

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4122 Clinical details: 1 year-old with diarrhoea and vomiting, family outbreak. Intended result: Adenovirus negative

Adenovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

115(100)53____

(100)

53 115____

2

Specimen : 4122 Adenovirus nucleic acid detection

UK (%) All (%)

2 (15.4) 2 (7.4)FTD0 (0.0) 1 (3.7)Gerbion0 (0.0) 1 (3.7)TIB MOLBIOL: LightMix0 (0.0) 1 (3.7)Ridagene1 (7.7) 1 (3.7)bioMerieux : R-gene1 (7.7) 1 (3.7)Unspecified2 (15.4) 2 (7.4)AusDiag: Easy-Plex2 (15.4) 2 (7.4)Real-Time Single target0 (0.0) 3 (11.1)Seegene: Allplex GI3 (23.1) 4 (14.8)Real-Time Multiplex0 (0.0) 4 (14.8)In house2 (15.4) 5 (18.5)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Adenovirus negative Equivocal Adenovirus positive Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus negative

27

0

Overall Results

------------------------------------------------------------

00

100% Correct13

------------------------------------------------------------

100

UK

270

0Adenovirus positive

13

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4122 Adenovirus antigen detection

UK (%) All (%)

2 (5.1) 2 (2.3)Remel : ProSpecT0 (0.0) 2 (2.3)Meridian: Rapid Strip 0 (0.0) 3 (3.4)Ridascreen1 (2.6) 3 (3.4)SD Bioline1 (2.6) 3 (3.4)bioMerieux : Vikia0 (0.0) 4 (4.5)DiaSorin : Liaison5 (12.8) 5 (5.7)Pro-Lab: Proflow1 (2.6) 6 (6.8)Unspecified3 (7.7) 10 (11.4)bioMerieux : bioNexia

10 (25.6) 11 (12.5)Biokit : Biorapid4 (10.3) 15 (17.0)Ridaquick8 (20.5) 16 (18.2)Coris Bioconcept

Number of reports0 4 8 12 16 20

Adenovirus positive Equivocal Adenovirus negative Unexpected virus

Your Report : Adenovirus negative

Adenovirus equivocal

0

Adenovirus positive

88

88

Overall Results

------------------------------------------------------------

390

100% Correct39

------------------------------------------------------------

100

UK

00

0Adenovirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 12: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 12

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4122 Clinical details: 1 year-old with diarrhoea and vomiting, family outbreak. Intended result: Norovirus positive

Norovirus positiveNorovirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(3.8)126(96.2)

79

763

(95.5)(4.5)

____132

____6

2-1

Specimen : 4122 Norovirus nucleic acid detection

UK (%) All (%)

3 (9.7) 3 (6.1)FTD1 (3.2) 1 (2.0)AusDiag: Easy-Plex0 (0.0) 1 (2.0)Gerbion0 (0.0) 1 (2.0)Seegene: Allplex GI2 (6.5) 2 (4.1)Cepheid: Smart3 (9.7) 3 (6.1)Rida Gene2 (6.5) 4 (8.2)Real-Time Multiplex3 (9.7) 4 (8.2)In house2 (6.5) 4 (8.2)bioMerieux: FilmArray2 (6.5) 4 (8.2)Unspecified

13 (41.9) 22 (44.9)Cepheid: GeneXpert

Number of reports0 6 12 18 24 30

Norovirus positive Norovirus negative

Your Report : Norovirus positive

Norovirus negative

100

Norovirus positive

31

Overall Results

49Total

0

------------------------------------------------------------UK

490

100

------------------------------------------------------------

31

All

% Correct

Score

Your Score : 2

Specimen : 4122 Norovirus antigen detection

UK (%) All (%)

2 (8.3) 6 (13.0)Rida Screen0 (0.0) 1 (2.2)Nadal 1 (4.2) 1 (2.2)Meridian: Rapid Strip 1 (4.2) 2 (4.3)SD Bioline4 (16.7) 4 (8.7)Pro-Lab: Proflow2 (8.3) 4 (8.7)Unspecified3 (12.5) 8 (17.4)ImmunoCard

11 (45.8) 20 (43.5)Rida Quick

Number of reports0 6 12 18 24 30

Norovirus positive Equivocal Norovirus negative

Your Report : Norovirus positive

Norovirus equivocal

24

Norovirus positive

6

Overall Results

46% Correct

31

84.8

------------------------------------------------------------UK

390

Total

Norovirus negative

21

All

87.5

------------------------------------------------------------

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13

Page 13: Viral gastroenteritis Laboratory : Distribution : 4196 Page 1 of 13 … · 2018. 8. 10. · UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12

Dispatch Date : 20-Nov-2017

Page 13

Viral gastroenteritis

Distribution : 4196

Laboratory :

Specimens 4121 and 4122 were positive for Norovirus genogroup II and prepared by pooling faecal materials. Specimen 4120 was positive for Rotavirus and was prepared from pooled faecal material and diluted 1:5. Specimen 4119 was negative for all three markers and was prepared using negative faecal material.

Specimen : 4122 Clinical details: 1 year-old with diarrhoea and vomiting, family outbreak. Intended result: Rotavirus negative

Rotavirus positiveRotavirus negative

Number of reports0 30 60 90 120 150

UK(%) All(%) Score

(98.8)1(1.2)

86

185

(0.7)(99.3)

____150

____149

-12

Specimen : 4122 Rotavirus nucleic acid detection

UK (%) All (%)

2 (16.7) 2 (8.0)FTD0 (0.0) 1 (4.0)Gerbion0 (0.0) 1 (4.0)TIB MOLBIOL: LightMix0 (0.0) 1 (4.0)Rida Gene1 (8.3) 1 (4.0)BD MAX2 (16.7) 2 (8.0)AusDiag: Easy-Plex0 (0.0) 2 (8.0)In house0 (0.0) 3 (12.0)Seegene: Allplex GI2 (16.7) 3 (12.0)Unspecified3 (25.0) 4 (16.0)Real-Time Multiplex2 (16.7) 5 (20.0)bioMerieux: FilmArray

Number of reports0 2 4 6 8 10

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus negative

Rotavirus equivocal

0

Rotavirus positive

25

24

Overall Results

------------------------------------------------------------

110

96.0% Correct12

------------------------------------------------------------

91.7

UK

10

0Rotavirus negative

1

All

Total

Unexpected virus

Score

Your Score : 2

Specimen : 4122 Rotavirus antigen detection

UK (%) All (%)

4 (6.1) 6 (5.1)Remel : ProSpecT1 (1.5) 3 (2.6)bioMerieux : Vikia2 (3.0) 4 (3.4)SD Bioline4 (6.1) 4 (3.4)Meridian: Premier0 (0.0) 4 (3.4)DiaSorin : Liaison7 (10.6) 7 (6.0)Pro-Lab: Proflow2 (3.0) 7 (6.0)Unspecified2 (3.0) 9 (7.7)bioMerieux : bioNexia

10 (15.2) 11 (9.4)Biokit : Biorapid13 (19.7) 14 (12.0)ImmunoCard

5 (7.6) 16 (13.7)Rida Quick10 (15.2) 18 (15.4)Coris Bioconcept

Number of reports0 4 8 12 16 20

Rotavirus positive Equivocal Rotavirus negative Unexpected virus

Your Report : Rotavirus negative

Rotavirus equivocal

0

Rotavirus positive

117

117

Overall Results

------------------------------------------------------------

660

100% Correct66

------------------------------------------------------------

100

UK

00

0Rotavirus negative

0

All

Total

Unexpected virus

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:10 on Thursday 12 July 2018

of 13