Veterinary Diagnostic Laboratory Impacting Iowa’s Animal Agriculture Industry 1st to identify and culture the new and highly virulent viruses Porcine Epidemic Diarrhea virus (PEDv) and Swine Delta Coronavirus in the U.S. in 2013 and 2014 • 1st to sequence and fully characterize PEDv in the U.S. • 1st to identify “where in the world” these new viruses came from Played a major role in diagnosis and response to Highly Pathogenic Avian Influenza virus (HPAI) in Iowa in 2015 • Partnered with IDALS, USDA and poultry producers to conduct tests for continuity of business during the outbreak • Conducted testing to allow re-population of sites 1st to identify an emerging vesicular disease (Senecavirus A, SVA) affecting U.S. swine. • Provided diagnostics for an unprecedented number of vesicular disease-associated foreign animal disease investigations to rule out foot-and-mouth disease in 2015 and 2016 • Urgent testing required to resume movement of pigs and sale of pork out of Iowa’s slaughter facilities vetmed.iastate.edu/vdl In 2013-2016, VDL faculty obtained nearly $4.2 million through 58 grants to advance knowledge on diagnosis and control of PEDV, HPAI, and SVA. QUESTIONS FROM CASES BECOME FUNDED RESEARCH PROJECTS Access to innovative diagnostic technologies are essential for supporting and growing Iowa’s export-centric animal agricultural industries. Investments in advanced diagnostic technologies have positioned the ISU VDL to effectively respond to introduction of new diseases in the last three years: Intestine from a PEDv-infected pig Comparing Genetic Similarities of SVA U.S. PEDv Prototype- like Strains U.S. PEDv S-INDEL-variant-like Strains 0.005 Comparing Genetic Similarities of PEDv PED virus ISU22376_Sioux_Chicken(H5N2) ISU23684_Wright_Chicken(H5N2) ISU21981_Kossuth_Chicken(H5N2) ISU21506_OBrien_Chicken(H5N2) ISU21233_Osceola_Chicken(H5N2) ISU21699_BuenaVista_Turkey(H5N2) ISU22716_Madison_Chicken(H5N2) KR233982_MN_Turkey_2015(H5N2) KR233998_OR_Chicken_2015(H5N2) KR233990_WA_Chicken_2015(H5N2) KR234030_KS_Chicken_2015(H5N2) KP307976_WA_NOPI_2014(H5N2) KP307984_WA_gyrfalcon_2014(H5N8) KJ509036_Korea_Baikal_teal_2014(H5N8) AB932556_Japan_Chicken_2014(H5N8) KJ509116_Korea_Chicken_2014(H5N8) KR232364_Netherlands_EAW_2015(H5N8) KR233690_Netherlands_Chicken_2014(H5N8) JQ973694_China_Duck_2010(H5N8) KJ476657_China_Mallard_2013(H5N8) KJ413834_Korea_Duck_2014(H5N8) KJ508961_Korea_Baikal_teal_2014(H5N8) NVSL1403_IN_Turkey_2016(H7N8) Indiana H7 2016 HPAI cases Iowa H5 2015 HPAI cases Comparing Genetic Similarities of HPAI AI virus SVA virus 0 20 40 60 80 100 120 2010 2011 2012 2013 2014 2015 2016 Number of Vesicular Disease Foreign Animal Disease Investigations Vesicles on the nose and feet of pigs with SVA. Senecavirus A U.S. contemporary strains Senecavirus A Brazilian contemporary strains Senecavirus A U.S. historical strains