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Feb 01, 2016
源資國際生物科技公司 業務專員 陳良坤
Vector NTI
Nucleotide analyses
Restriction site analysis
Design Primers
Align
Assemble
Vector NTI
Nucleotide analyses
Restriction site analysis
Design Primers
Align
Assemble
Vector NTI SuiteVector NTI Suite
Graphics Pane
Main Menu
Main Toolbar
Pane Selection Toolbar
Pane Toolbar
Text Pane
Sequence Pane
GenBank/GenPetp
File
Select all sequence →Right-click mouse →Reverse selection
Analyses →ORF
Select ORF→Analyses →Into New Protein →Direct Strand
→Analyses →Restriction Analyses →Restriction Sites
pUC 1926 8 6 bp
A P r
A L P H AP (B L A )
P (L A C )
O R I
Ava I (4 13)
Bam H I (4 18 )
Eco R I (39 7)
H ind III (4 4 8 )
P st I (4 4 0 )
Sm a I (4 15)
X m a I (4 13)
Apa L I (178 )
Apa L I (1121)
Apa L I (236 7)
H um a n h e m o g lob in20 40 bp
N coI (1130 )
N coI (140 9)P s tI (258 ) P s tI (78 5)
P s tI (138 0 )
Sm a I (292)
Sm a I (1237)
Sm a I (1666)
X m a I (290 )
X m a I (1235)
X m a I (1664)A vaI (290 )
A vaI (1153)
A vaI (1235)
A vaI (1664)
pUC 19334 6 bp
A P r
A L P H A
In s e r t fr a g m e n t
P (B L A )
P (L A C )
O R I
Ba m H I (4 18 )
EcoR I (39 7)
H ind III (110 8 )
Nco I (4 6 9 )
P st I (4 4 0 )
Ava I (4 13)
Ava I (724 )
Sm a I (4 15)
Sm a I (726 )
X m a I (4 13)
X m a I (724 )
Bg lI (252)
Bg lI (24 8 0 )
X ba I (4 24 )
X ba I (4 8 8 )
Ap a L I (178 )
Ap a L I (178 1)
Ap a L I (30 27)
by PstI
Select region→Analyses →Primer Design →Find PCR Primers
Select primer→Right-click mouse → Analyses
Select file
Align→AlignX-Align Selected Molecules
Assemble→ContigExpress-Assemble Selected Sequence
Open ContigExpress Project→Project→Add Fragments→From ABI file (no Chinese)
→ Right-click mouse→Camera
→Select sequence → Right-click mouse→Copy sequence…..