I. Utility and use of zebrafish as model for understanding angiogenesis. II. VEGF signaling in zebrafish during angiogenesis. III.Mathematical modeling of angiogenesis Cell signaling, endothelial migration, and zebrafish: a simplified model for angiogenesis Khalid Boushaba, Jeffrey Essner, and Howard Levine Iowa State University
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Utility and use of zebrafish as model for understanding angiogenesis.
Cell signaling, endothelial migration, and zebrafish: a simplified model for angiogenesis Khalid Boushaba, Jeffrey Essner, and Howard Levine Iowa State University. Utility and use of zebrafish as model for understanding angiogenesis. VEGF signaling in zebrafish during angiogenesis. - PowerPoint PPT Presentation
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I. Utility and use of zebrafish as model for understanding angiogenesis.
II. VEGF signaling in zebrafish during angiogenesis.
III. Mathematical modeling of angiogenesis
Cell signaling, endothelial migration, and zebrafish: a simplified model for angiogenesis
Khalid Boushaba, Jeffrey Essner, and Howard LevineIowa State University
Cell signaling, endothelial migration, and zebrafish: a simplified model for angiogenesis
Zebrafish as a High-throughput Model for Angiogenesis Research and Therapeutic Development
Large number of offspringOptically clear embryosShort generation timeSmall SizeForward Genetics:
ENU mutagenesisInsertional mutagenesis
Reverse Genetics:Transgenic fishTilling with ENUMorpholino injection
Genomics:Sequenced GenomecDNA projectsMicroarrays
Small Molecule Screens:Predictive of higher vertebratesDelivery by injection or soaking
Carcinogenesis: Aqueous deliverySimilar to human tumors
Zebrafish embryos are optically clear and develop rapidly
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From Karlstrom and Kane, 1996
From Yancopoulos et al., 2000
Model of Tumor Angiogenesis
Novel Angiogenic
Factors Candidate Anti-Tumor
Agents
Advantages of Studying Angiogenesis in Zebrafish
Angiogenesis is a conserved vertebrate-specific function
Analysis in living embryos
2.7 dpf
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Transgenic zebrafish allow analysis of endothelial cells in living embryos