US EPA-Pesticides;Trifluralin | US EPA ARCHIVE DOCUMENT · CAS name 2,6-Dinitro-N,N-dipropyl-4-(trifluoromethyl)benzena~e CAS NO. 1582-09-8 Synonyms Primary Reviewer: Moncie Wright

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on Earthworms PMRA Submission #: { ............ 1 EPA MRID #: 47807009

............ Data Requirement: PMRA Data Code { 1 EPA DP Barcode 367525 OECD Data Point { ............ 1 EPA MRID 47807009 EPA Guideline 850.6200; OECD 207

Test material: Trifluralin Technical Purity: 96.2% wlw Common name Chemical name: IUPAC a,a,a-Trifluoro-2,6-dinitro-N,N-dipropyl-p-toluid~e

CAS name 2,6-Dinitro-N,N-dipropyl-4-(trifluoromethyl)benzena~e CAS NO. 1582-09-8 Synonyms

Primary Reviewer: Moncie Wright Staff Scientist, Cambridge Environmental

Secondary Reviewer: Teri S. Myers Senior Scientist, Cambridge Environmental

Signature: Date: 11/3/09

Signature: h ' 5 Date: 12/07/09

Primary Reviewer: Chri EPA/OPP/EFED/ERB 1

...................................... ............ Secondary Reviewer(s): { 1 Date: { 1 {EPAJOECDE'MRA}

ReferenceISubmission No. { ..................... 1

Company Code { ................ ) [For PMRA] Active Code { .............. ..) [For PMRA] EPA PC Code 036101

Date Evaluation Completed: 4130110

CITATION: Rodgers, Matthew H. 1999. Trifluralin Technical: Acute Toxicity (LC5,)) to the Earthworm (Eisenia foetida). Unpublished study performed by Huntingdon Life Sciences Limited, Cambridgeshire, England. Laboratory study ID: DWC 9841992368. Study sponsored by Dow AgroSciences, Letcombe Regis, Oxon, England. Sponsor study ID: GHE-T-954. Study completed April 29, 1999.

TEXT SEARCHABLE DOCUMENT - 2010

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on Earthworms PMRA Submission #: (............ 1 EPA MRID #: 47807009

EXECUTIVE SUMMARY: In an acute toxicity study, earthworms (Eisenia foetida) were exposed to Trifluralin Technical at nominal concentrations of 0 (solvent control), 95, 171,309,556, and 1000 mg aikg dw soil (artificial substrate). No negative control group was included in the study. The reference chemical used was chloroacetamide (concentrations not reported), which had an LC50 value of 46 mgtkg dw soil.

After 14 days of exposure, mortality was 3% in the negative control, and was 8,0,3,3, and 0% in the nominal 95,171, 309,556, and 1000 mg aikg dw soil treatment groups, respectively. Survival was not affected, yielding an LCs0 value of >lo00 mg aikg dw soil, respectively. Mean % weight gain was 2 1% in the control, and was 1 1, -1, -2, -7, and -9% in the nominal 95, 171, 309, 556, and 1000 mg aikg dw soil treatment groups, respectively. The NOAEC and ICS0 values, based on body weight change, were <95 and >I000 mg aikg dw soil, respectively. The LOAEC value, based on % weight loss, was 95 mg aikg dw soil.

Test organisms appeared to be in good health throughout the study.

This study is scientifically sound and classified as a Supplemental non-guideline study (although it follows OECD 207, EPA does not have a guidelinesfor a 14-day earthworm test).

Results Synopsis

Test Organism Size/Age(Mean Wt or Length): 363-368 mg (based on group mean values at study initiation)

Mortality LCs0: >lo00 mg ailkg dw soil 95% C.I.: NIA NOAEC: 1000 mg aikg dw soil

Sublethal (weight gain) IC50: >lo00 mg ailkg dw soil 95% C.I.: NIA NOAEC: <95 mg aikg dw soil; based on % weight gain

Endpoint(s) Affected: % weight gain (day 0 to day 14)

Page 2 of 15


I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study was conducted following OECD Guideline 207 for Testitpg of Chemicals, "Earthworm, acute toxicity tests" (1984), and EEC Direbtive 87/302/EEC, Part C, Methods for determination of ecotoxicity, "Tobicity for earthworms: Artificial soil test". The test was only conducted for 141 days; the reviewer used the guidance for a 28-day sub-chronic test to note deyiations in acclimation and environmental conditions. The following deviations fiom OPPTS Guideline 850.6200 were noted:

1. Only a solvent control was tested with the treatment groups in this study, which is in accordance with OECD guidelines. However, OPPTS guidelines suggest that a negative co~+trol be tested along with a solvent control to determine whether the solvent might have had an mdue effect on the test results. In the solvent control group, worm body weight had a 21% increase after 14 days.

2. The % organic carbon content of the soil was not reported. I

3. Only the pH of the soil before treatment was reported; OPPTS guidelines suggestl that daily pH values be reported.

4. The concentrations of the test mixtures were not analyzed; OPPTS guidelines sukgest that test concentrations be measured daily.

5. The study author did not report quarantining and observing the earthworms; O P P ~ S guidelines suggest this should occur for at least 14 days prior to testing. I

I

6. Acclimation was not officially reported in the MRID; the only mention of accllmlation is in the study protocol, where the study author reports that the worms will be acclimated tc/ the soil for at least 24 hours.

7. The pretest health of the earthworms was not reported.

8. The concentration of the solvent used for testing was not reported.

These deviations do impact the acceptability of the study.

COMPLIANCE:

A. MATERIALS:

1. Test Material

Description:

Signed and dated No Data Confidentiality, Quality Assurance and GLP statements were provided. This study was conducted in compliancq with U.S. EPA Title 40 Code of Federal Regulations Part 160 (1989), and with OECD principles (ENlMClCHEM(98) 17; 1998).

Trifluralin Technical

Orange crystalline powder

Page 3 of 15


Lot No./Batch No. : RMM 1915 (batch no.)

Purity: 96.2% wlw

Stability of compound under test conditions: Analytical verification was not performed.

(OECD recommends water solubility, stability in water and light, pKa, Pow, vapor pressure of test compound)

Storage conditions of test chemicals: The test material was stored at room temperature in the dark.

Physicochemical properties of Trifluralin Technical. I I

Parameter

Water solubility a t 20EC

I W absorption I Not reported I I Vapor pressure

Values

Not reported

Not reported

1 Kow I Not reported I I

Comments

I I

P K ~

2. Test organism:

Not reported

Species: Earthworm (Eisenia foetida) (EPA and OECD recommend Eisenia fetida andvei (Bouche). The earthworms should weigh 300-600 mg at the beginning of the test.)

I I

Age a t test initiation: Adult

Weight a t study initiation: 363-368 mg (based on group mean values at study initiation)

Source: Brickyard Farm, Buckworth Cambridgeshire, UK

B. STUDY DESIGN:

1. Experimental Conditions

a. Range-finding Study: A range-finding study was not reported.

b. Definitive Study

1. Soil

Page 4 of 15


For natural soil: Texture: % sand

% silt % clay

Textural classification

Table 1: Physicochemical Properties of Natural Soil

For artificial substrate (provide

Property

composition):

Value

Artificial soil used in the definitive tests was comprised of 10% sphagnum peat, 20% Kaolin clay, and 70% industrial quartz sand. Calcium carbonate was added to the soil mixture to obtain a pH of 5.7. The initial soil moisture content before treatment was not reported. The % organic carbon was not reported.

11 Organic carbon (%) I Moisture (%)

Table 2: Experimental Design

l l I Parameter

Acclimation:

11 duration: conditions (state if same as the test conditions): health:

11 Soil [fiesh or stored]

Test Container

material

Remarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

criteria li

Recommended testing medium is artiJicia1 soil consisting of a mixture of 68% of No 70 mesh silica sand, 20% kaolin clay, I0 sphagnum peat moss, and 2% calcium carbonate, mixed and moistened to 35% by weight with deionizeddistilled water.

None reported; refer to adjacent Remarks

NIA

NIA Not reported

Detail

The study author reported in the protocol that the worms will be acclimated to the soil for at least 24 hours.

Worms were received on 16 October 1998; test initiated on 9 December 1998. No other details of acclimation provided.

Remarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Criteria

Earthworms should be acclimated at test temperature for 7 days.

Freshly prepared

Page 5 of 15


Parameter

size amount of soillsubstrate

No. of replicates:

per treatment group: per control:

No. of earthworms per treatment

Solvents used or not (if yes report the name and concentration)

Rates of application: nominal:

measured:

Reference chemical (if used) name: concentration:

Test conditions:

temperature Lighting conditions

Moisture

Duration of the study

Detail

Glass

1 L

Average of 740 g wet weight

4

4

40 total for each treatment group and the control

Acetone; concentration not reported

(solvent control), 95, 171, 3097 5567 and loo' mg dw

NIA

Chloroacetamide

Not reported

22-23°C

Continuous

520 lwc

Moisture was 34 to 35% of the dry weight at test initiation. After 14 days, moisture content was 30% of the soil dry weight.

14 days

Remarks -----.---------------------------.....--.

Criteria

- - - ~ - - - - - ~ - - ~ ~ ~ - - ~ - ~ ~ - - ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ - ~ ~ .

Recommended number of replicates include at least 3 and a control

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - .

Recommended number of earthworms per treatment include a minimum of 30 plus a control, 10 per each of three replicates and a control.

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ---------- . Earthworms should be exposed to at hastfive test concentratrons, in geometric serzes, zn whzch the ratzo zs between 1.5 and 2.0 mg of test chemical per kg (air-dv weight) of artEficial soil.

.--.--.-...--...------------------------.

Recommended temperature. 22 + 2OC Recommended lightzng. Continuous zllumrnation, with a light intensity of 400 lux Recommended relative humzdity above 85%

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .


rable 3: Observations

Parameters

Observation intervals

Remarks ----------------------------------------.

Criteria I

Recommended duration of ptudy is 28 days.

Parameter

Parameters measured including the sublethal effectsltoxicity symptoms

I r 2. Observations:

Detail

Were raw data included?

Other observations, if any

11. RESULTS AND DISCUSSIONS

A. MORTALITY:

Details

Mortality and weights of the worms as replicate and group mean weights of worms were assessed on days 7 and 14. Behavioral abnormalities and pathological signs were observed daily.

-Mortality -Weight of surviving earthworms (weight at days 0,7, and 14) -Sub-Lethal Effects (behavior and pathological signs)

Yes, number of mortalities per replicate, average worm weight for each replicate on day 0, 7, 14

None

Remarks I

Criteria

Recommended observation intervals are days 7, 14,221, and 28.

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

The test is usually not acaeptable i f more than 20% of control earthworms die or the total mean weight of control earthworms lose 20% or more of body weight.

After 14 days of exposure, mortality was 3% in the solvent control, and was 8,0,3,3, and 0% in the nominal 95, 171,309,556, and 1000 mg aikg dw soil treatment groups, respectively. The study author reported a 14- day LCso value of >I000 mg aikg dw soil.

Page 7 of 15


rable 4: Effect of Triflurali

Nominal Concentrations (mg ailkg soil)

Solvent Control

NOAEC

LOAEC

LC50

Reference chemical % mortality: LCSo (95% confidence limits)

Not reported 11

R Technical on Mortality of Eisenia foetida

Observation period

>lo00 mg ai/kg dw soil II 46 (44-50) mgkg

Day 7

46 (44-50) mgkg

Not reported

No Dead

1

2

0

1

1

0

Day 14

B. SUB-LETHAL TOXICITY ENDPOINTS:

% mortality

3

5

0

3

3

0

No Dead

1

3

0

1

1

0

The study author analyzed mean worm weights at day 7 and 14 with Williams' test and reported replicate mean values. Mean % weight loss was -21% in the negative control, and was -1 1, l , 2 ,7 , and 9% in the nominal 95, 17 1,309, 556, and 1000 mg ai/kg dw soil treatment groups, respectively.

% mortality

3

8

0

3

3

0

Test organisms appeared to be in good health throughout the study.

Page 8 of 15

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on Earthworms PMRA Submission #: (............ 1 EPA MRID #: 47807009

Table 5: Sub-lethal Effect of Trifluralin Technical on Ehenia foetida. Average weights were used

C. REPORTED STATISTICS:

Nominal Concentrations (mg ailkg soil)

Control

95

171

309

556

1000

NOAEC

LOAEC

EC5o

Mortality endpoints were visually determined due to only an 8% effect at the highest test level. A qne-way analysis of variance was carried out on the mean bodyweight per replicate at Day 7 and Day 14. Thie mean bodyweight at Day 0 was included as a covariate in the analyses as this improved precision (covarihte efficiency> 100%). The analysis was followed by Williams' test (Williams 197 1, Williams 1972) for contrasting increasing dose levels of the compound with a zero dose control. Analyses were performed using Genstat 5.3.2.

The study author reported: "Mean bodyweights increased in all groups over Days 0 to 7, and decreased in all groups, apart frob the control, over Days 7 to 14. On Days 7 and 14 mean bodyweights were lower in all treated grouljs than in the control.

Observation period

Mean bodyweights at Days 7 and 14 were analysed statistically. At Day 7, bodyweights were significantly reduced in comparison with the control at 171 mglkg dry soil (p<O.05), and at 309, 556 and 1000 mglkg dry soil (p<0.01). At Day 14, mean bodyweights were significantly reduced in comparison with the control in all groups treated with trifluralin technical (p<0.01)."

Reference chemical % mortality: LC50 (95% confidence limits)

Page 9 of 15

Day 0

< 17 1 mg aikg dw soil

Not reported

Not reported

weight

363

3 64

368

3 64

3 65

3 64

At 7 days : 46 (44-50) mgtkg

Day 7

% loss

NIA

NIA

NIA

NIA

NIA

NIA

At 14 day: 46 (44-50) mgkg

weight

440

433

417

404

395

377

Day 14

% gain (day 0 to day 7)

2 1

19

13

11

8

4

weight

43 9

404

3 64

356

340

333

% gain (day 7 to day 14)

0

-7

-13

-12

-14

-12

% gain (day 0 to

I day 14) I

2 1

11

- 1

I -2 I

-7

-9

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on Earthworms PMRA Submission #: { ............ EPA MRID #: 47807009

D. VERIFICATION OF STATISTICAL RESULTS:

Statistical Method: All analyses were conducted using the nominal concentrations. The study author did not include a negative control in this study; therefore, the reviewer conducted all analyses using the solvent control.

Mortality did not reach 50%, therefore the LCso value was visually determined as greater than the highest test concentration (21000 mg ailkg dw soil). The NOAEC based on mortality was visually determined at 1000 mg ailkg dw soil.

The % change in weight also did not reach 50%, therefore the IC50 value was also visually determined (>I000 mg aikg-dw soil). The reviewer tested the percent weight gain data (from day 0 to day 14) for normality using the Shapiro-Wilks tests and for homogeneity of variance using the Bartlett's tests via Toxstat 3.5 statistical software. The data met the assumptions of ANOVA, thus the NOAEC value was determined using the parametric Williams' tests via Toxstat statistical software. All analyses were conducted using the nominal concentrations.

Williams' test detected a significant difference (p<0.05) at every test level.

Mortality LC50: >I000 mg ailkg dw soil 95% C.I.: NIA NOAEC: 1000 mg ailkg dw soil

Sublethal (weight gain) IC50: >I000 mg ailkg dw soil 95% C.I.: NIA NOAEC: <95 mg ailkg dw soil; based on % weight gain


E. STUDY DEFICIENCIES:

Only a solvent control was tested with the treatment groups in this study, which is in accordance with OECD guidelines. However, OPPTS guidelines suggest that a negative control be tested along with a solvent control to determine whether the solvent might have had an undue effect on the test results. In the solvent control group, worm body weight had a 21% promotion of growth based on weights. The reviewer could not determine if the solvent had confounding effects on the experimental results.

F. REVIEWER'S COMMENTS:

The reviewer's and study author's results were in complete agreement.

No OPPTS guidance exists for a 14-day acute earthworm toxicity test, thereby making this a non-guideline test. Therefore, the reviewer used the guidance for a 28-day sub-chronic test to note deviations in acclimation and environmental conditions.

The % organic carbon content of the soil was not reported.

Only the pH of the soil before treatment was reported; OPPTS guidelines suggest that daily pH values be reported.

The concentrations of the test mixtures were not analyzed; OPPTS guidelines suggest that test concentrations be measured daily.

Page 10 of 15

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on Earthworms PMRA Submission #: { ............ 1 EPA MRlD #: 47807009

The concentration of the solvent used for testing was not reported.

The pretest health of the earthworms was not reported.

The study author did not report quarantining and observing the earthworms; OPPTS guidelines suggdst this should occur for at least 14 days prior to testing.

Acclimation conditions were not reported in the study; the only mention of acclimation is in the stltdy protocol, where the study author reports that the worms will be acclimated to the soil for at least 24 hours.

The experiment was conducted from December 9 to 23,1998.

G. CONCLUSIONS:

This study is scientifically sound and classified as a Supplemental non-guideline study (although it follows OECD 207, EPA does not have a guideline for a 14-day earthworm test). Percent survival was not affected, yielding NOAEC and values of 1000 and >I000 mg ailkg dw soil, respectively. Body weight gain was affected in all test levels, yielding NOAEC and ICso values of <95 and >lo00 mg aikg dw soil, respectively.

Mortality LC50: >I000 mg aiikg dw soil 95% C.I.: NIA NOAEC: 1000 mg ai/kg dw soil

Sublethal (weight gain) ICsO: >I000 mg ailkg dw soil 95% C.I.: NIA NOAEC: <95 mg ailkg dw soil; based on % weight gain


111. REFERENCES:

Berkson, J. (1944) Application of the logistic function to bio-assay. J. Amer. Statist. Assoc. 39,357-365.

Williams, D.A. (1986) Interval Estimation of the median Lethal Dose. Biometries, 42, 641-645.

Page 11 of 15


APPENDIX I. OUTPUT OF REVIEWER'S STATISTICAL VERIFICATION:

Trifluralin & earthworm 14-day % weight gain; mg ai/kg File: 7009w Transform: NO TRANSFORMATION

Chi-square test for normality: actual and expected frequencies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

INTERVAL <-I. 5 -1.5 to <-0.5 -0.5 to 0.5 20.5 to 1.5 >1.5

EXPECTED 1.608 5.808 OBSERVED 0 8

Calculated Chi-square goodness of fit test statistic = 6.3102 Table Chi-Square value (alpha = 0.01) = 13.277

Data PASS normality test. Continue analysis.


Shapiro Wilks test for normality . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Critical W (P = 0.05) (n = 24) = 0.916 Critical W (P = 0.01) (n = 24) = 0.884 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Data PASS normality test at P=0.01 level. Continue analysis.


Hartley test for homogeneity of variance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Calculated H statistic (max ~ar/min Var) = 59.19 Closest, conservative, Table H statistic = 184.0 (alpha = 0.01)

Used for Table H ==> R ( # groups) = 6, df ( # reps-1) = 3 Actual values ==> R ( # groups) = 6, df ( # avg reps-1) = 3.00

Data PASS homogeneity test. Continue analysis.

NOTE: This test requires equal replicate sizes. If they are unequal but do not differ greatly, the Hartley test may still be used as an approximate test (average df are used).

Page 12 of 15

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on


Bartletts test for homogeneity of variance - - - - - _ _ ~ _ - - _ _ _ _ _ ~ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Calculated B statistic = 10.99 Table Chi-square value = 15.09 (alpha = 0.01) Table Chi-square value = 11.07 (alpha = 0.05)

Average df used in calculation ==> df (avg n - 1) = 3.00 Used for Chi-square table value ==> df (#groups-1) = 5 - - - - _ _ _ - _ _ _ _ _ _ _ _ ~ _ _ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Data PASS homogeneity test at 0.01 level. Continue analysis.

NOTE: If groups have .unequal replicate sizes the average replicate size is used to calculate the B statistic (see above).

Title: Trifluralin & earthworm 14-day % weight gain; mg ai/kg File: 7009-WT .TXT Transf o m : NO TRANSFORMATION

ANOVA Table - - - - - - - - _ _ _ - - - - _ _ _ _ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

SOURCE DF SS MS F - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Between 5 2688.8333 537.7667 22.2780

Within (Error) 18 434.5000 24.1389 _ _ _ ~ _ - - - - - _ _ _ _ _ ~ _ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Total 23 3123.3333 _ _ _ _ _ _ _ _ _ - ~ _ _ _ _ _ _ _ _ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

(p-value = 0.0000)

Critical F = 4.2479 (alpha = 0.01, df = 5,181 = 2.7729 (alpha = 0.05, df = 5,181

Since F > Critical F REJECT Ho: All equal (alpha = 0.05)

Title: Trifluralin & earthworm 14-day % weight gain; mg ai/kg File : 7009-WT .TXT Transform: NO TRANSFORMATION

Dunnett's Test - TABLE 1 OF 2 Ho:Control<Treatment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05 - - - - - . . . . . . . . . . . . . . . . . . . . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 Sol control 21.0000 21.0000 2 9 5 11.2500 11.2500 2.8065 * 3 171 -1.0000 -1.0000 6.3326 * 4 309 -2.5000 -2.5000 6.7643 * 5 556 -7.0000 -7.0000 8.0596 * 6 1000 -8.7500 -8.7500 8.5633 *

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05, df = 5,181

Page 13 of 15


Title: Trifluralin & earthworm 14-day % weight gain; mg ai/kg File: 7009-WT .TXT Transform: NO TRANSFORMATION

Dunnett's Test - TABLE 2 OF 2 Ho:Control<Treatment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

NUM OF MIN SIG DIFF % OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL - - - - - . . . . . . . . . . . . . . . . . . . . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 Sol control 4 2 95 4 8.3726 39.9 9.7500 3 171 4 8.3726 39.9 22.0000 4 3 0 9 4 8.3726 39.9 23.5000 5 556 4 8.3726 39.9 28.0000 6 1000 4 8.3726 39.9 29.7500

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Title: Trifluralin & earthworm 14-day % weight gain; mg ai/kg File : 7009JT .TXT Transform: NO TRANSFORMATION

William's Test - TABLE 1 OF 2 Ho: ControlcTreatment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

ORIGINAL TRANSFORMED ISOTONIZED GROUP IDENTIFICATION N MEAN MEAN MEAN - - - - - - . . . . . . . . . . . . . . . . . . . . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

1 Sol control 4 21.0000 21.0000 21.0000 2 95 4 11.2500 11.2500 11.2500 3 171 4 -1.0000 -1.0000 -1.0000 4 309 4 -2.5000 -2.5000 -2.5000 5 556 4 -7.0000 -7.0000 -7.0000 6 1000 4 -8.7500 -8.7500 -8.7500

Page 14 of 15

Data Evaluation Report on the Acute Toxicity Effects of Trifluralin Technical on

Title: Trifluralin & earthworm 14-day % weight gain; mg ai/kg File: 7009-WT .TXT Transform: NO TRANSFORMATION

William's Test - TABLE 2 OF 2 Ho: Control<Treatment _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

COMPARED CALC . SIG TABLE DEGREES OF IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM USqD

_ _ _ _ _ _ _ - _ _ _ _ _ _ _ _ _ _ - _ _ _ _ _ _ - _ _ _ _ _ _ _ _ _ _ _ _ - - - - - _ _ _ _ - - - - - - - _ - _ _ - - - - - - - - - - _ - Sol control 21.0000

95 11.2500 2.8065 * 1.7300 k= 1, v=18 171 -1.0000 6.3326 * 1.8200 k= 2, v=18 309 -2.5000 6.7643 x 1.8500 k= 3, v=18 556 -7.0000 8.0596 * 1.8600 k= 4, v=18 1000 -8.7500 8.5633 x 1.8700 k= 5, v=&8

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US EPA-Pesticides;Trifluralin | US EPA ARCHIVE DOCUMENT · CAS name 2,6-Dinitro-N,N-dipropyl-4-(trifluoromethyl)benzena~e CAS NO. 1582-09-8 Synonyms Primary Reviewer: Moncie Wright

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