URINE SEDIMENTS DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSIS FATIMA COLLEGE OF MEDICINE.

URINE SEDIMENTSURINE SEDIMENTS

DEPARTMENT OF PATHOLOGY DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSISAND LABORATORY DIAGNOSIS

FATIMA COLLEGE OF FATIMA COLLEGE OF MEDICINEMEDICINE


RED BLOOD CELLS. UNSTAINED, X400.


RED BLOOD CELLS. STERNHEIMER STAIN, X400.


WHITE BLOOD CELLS. UNSTAINED, X400.


WHITE BLOOD CELLS. STERNHEIMER STAIN, X400.


SQUAMOUS EPITHELIAL CELLS (SUPERFICIAL TYPE). CYTOLASM IS THIN AND WRINKLED. CELL PERIPHERY

IS FOLDED. UNSTAINED, X400.


SQUAMOUS EPITHELIAL CELLS (SUPERFICIAL TYPE). STAINABILITY OF THE CELLS ARE GOOD. THE NUCLEI ARE SMALL AND CONDENSED. STERNHEIMER STAIN,

X400.


SQUAMOUS EPITHELIAL CELLS (INTERMEDIATE TYPE). THE CYTOPLASM IS THICKER THAN THAT OF

SUPERFICIAL TYPE CELLS. CELL PERIPHERY IS CLEAN AND ROUND. UNSTAINED, X400.


SQUAMOUS EPITHELIAL CELLS (INTERMEDIATE TYPE). STAINABILITY IS GENERALLY POOR. THE CELLS STAIN

LIGHT PINK. STERNHEIMER STAIN, X400.


SQUAMOUS EPITHELIAL CELLS (DEEP LAYER TYPE). THE CYTOPLASM IS THICK AND HOMOGENOUS. CELL

SHAPE IS MOSTLY SPHERICAL. UNSTAINED, X400.


SQUAMOUS EPITHELIAL CELLS (DEEP LAYER TYPE). STAINABILITY IS GENERALLY POOR. STERNHEIMER

STAIN, X400.


TRANSITIONAL EPITHELIAL CELLS (SUPERFICIAL TYPE). THEY HAVE THICKER CYTOPLASM. THE

PERIPHERAL IS CRESCENT- SHAPED, POLYGONAL AND ANGULAR. UNSTAINED, X400


TRANSITIONAL EPITHELIAL CELLS (SUPERFICIAL TYPE). THE SURFACE STRUCTURE IS ROUGH. THE

CELLS HAVE GENERALLY HAVE 2 OR 3 NUCLEI AND THEY ARE LARGE. STERNHEIMER STAIN, X400.


TRANSITIONAL EPITHELIAL CELLS (INTERMEDIATE TYPE). THE CYTOPLASM SHOWS A YELLOWISH TONE. SURFACE STRUCTURE OF THE CELLS IS ROUGH. THE

PERIPHERAL IS ANGULAR. UNSTAINED X400.


TRANSITIONAL EPITHELIAL CELLS (INTERMEDIATE TYPE). STAINABILITY IS GOOD, RESULTING IN A PINK

VIOLET STAIN. STERNHEIMER STAIN X400.


TRANSITIONAL EPITHELIAL CELLS (DEEP LAYER TYPE). THE CYTOPLASM IS GENERALLY THINNER THAN IN DEEP LAYER

TYPE SQUAMOUS EPITHELIAL CELLS. THE PERIPHERY OF THE CELLS IS ANGULAR. THE UNSTAINED CYTOPLASM SHOWS A

YELLOWISH TONE. UNSTAINED, X400.


TRANSITIONAL EPITHELIAL CELLS (DEEP LAYER TYPE). STAINABILITY IS GOOD. STERNHEIMER STAIN, X400.


RENAL TUBULAR EPITHELIAL CELLS. THE LARGE AND IRREGULAR CELLS ARE RENAL TUBULAR EPITHELIAL

CELLS. THE NUCLEI ARE SMALL AND UNEVENLY LOCATED. UNSTAINED, X400.


RENAL TUBULAR EPITHELIAL CELLS. STAINABILITY IS GOOD. THE PERIPHERAL STRUCTURE IS INDENTED.

STERNHEIMER STAIN, X400.


OVAL FAT BODY. THE FAT GRANULES SHOW A YELLOWISH BROWN GLOSS OR DARK

BROWN GLOSS. UNSTAINED, X400.


OVAL FAT BODY. FAT GRANULES STAIN RED. SUDAN III STAIN, X400


OVAL FAT BODY. THE SO-CALLED MALTESE CROSS DOUBLE REFRACTIVE IMAGE IS OBSERVED. POLARIZING MICROSCOPE

IMAGE, X400.


HYALINE CAST. NO INCLUSION IS SEEN IN THE CAST. UNSTAINED, X400.


HYALINE CAST. NO INCLUSION IS SEEN IN THE CAST. STERNHEIMER STAIN, X400.


HYALINE CAST. CHARACTERIZED BY A SMALL QUANTITY OF FINE GRANULES

INCLUDED IN THE CAST. STERNHEIMER STAIN, X400.


EPITHELIAL CELL CAST. MANY RENAL TUBULAR EPITHELIAL CELLS ARE SEEN IN

THE CAST. UNSTAINED, X400.


EPITHELIAL CELL CAST. MANY RENAL TUBULAR EPITHELIAL CELLS COMPLETELY

FILL THE CAST. STERNHEIMER STAIN, X400.


GRANULAR CAST. THE CAST CONTAINS MANY COARSE GRANULES. UNSTAINED,

X400.


GRANULAR CAST. ROUGH GRANULES ACCOUNT FOR MOST OF THE CAST.

STERNHEIMER-MALBIN STAIN, X400.


WAXY CAST (BROAD CAST). THE CAST SHOW A CHARACTERISTIC FISSURE. THE CAST IS

VERY BROAD WHEN COMPARED WITH SURROUNDING CELLS. UNSTAINED, X400.


WAXY CAST. THE CAST ALSO HAS A FISSURE AND WAXY SURFACE, BUT THE WIDTH IS NOT SO GREAT. STERNHEIMER

STAIN, X400.


FATTY CAST. FATTY GRANULES ARE TIGHTLY PACKED IN THE CAST. UNSTAINED, X400.


FATTY CAST. FATTY GRANULES DO NOT STAIN, BUT THE CAST AS A WHOLE, STAIN ORANGE-YELLOW COLOR. STERNHEIMER

STAIN, X400.


FATTY CAST. FATTY GRANULES STAIN ORANGE RED COLOR. SUDAN III STAIN,

X400.


RBC CAST. THE CAST SHOW A YELLOW-BROWN COLOR REFLECTING THE

HEMOGLOBIN PIGMENT. UNSTAINED, X400.


RBC CAST. RBC’S INCLUDED IN THE CAST STAIN LIGHT RED AND CAN BE CLEARLY DISTINGUISHED. STERNHEIMER STAIN,

X400.


WBC CAST. THIS CAST CONTAINS MANY WBC’S. UNSTAINED, X400.


WBC CAST. THIS CAST CONTAINS MANY WBC’S INCLUDING SEGMENTED

NEUTROPHIL. STERNHEIMER STAIN, X400.


BACTERIA. MANY ROD-SHAPED BACTERIA ARE OBSERVED. WBC’S ARE ALSO PRESENT. THIS SPECIMEN IS REPORTED AS “BACTERIA

(+++)”.


YEAST. THE SHAPES ADOPTED BY YEAST ARE OVOID, GRAINY AND BUDDING. THIS

SPECIMEN IS REPORTED AS “YEAST (+++)”.


TRICHOMONAS. FLAGELLATED TRICHOMONAS WHICH MOVE ACTIVELY ARE

SEEN. THEY ARE GLOSSY AND A LITTLE LARGER THAN WBC. THIS SPECIMEN IS REPORTED AS “TRICHOMONAS (+++)”.


CALCIUM OXALATE CRYSTALS. COLORLESS, REGULAR OCTAHEDRAL. PH 6.0.

UNSTAINED, X400.


CALCIUM OXALATE CRYSTALS. BISCUIT-SHAPED. THEY MAY BE CONFUSED WITH RED BLOOD CELLS. PH 6.0, UNSTAINED,

X400.


URIC ACID CRYSTAL. THEY APPEAR YELLOW-BROWN IN COLOR. IN THIS EXAMPLE, LARGE

LENS-SHAPED CRYSTALS OVERLAP EACH OTHER. PH 6.0, UNSTAINED, X400.


URIC ACID CRYSTAL. NOTICE THE YELLOW COLOR AND VARIETY OF SHAPES. X400.


URIC ACID CRYSTAL, POLARIZED. X400.


CALCIUM PHOSPHATE CRYSTAL. THIS IS WHITISH GRAY, IRREGULAR PLATE FORM.

PH 8.0, UNSTAINED, X400.


TRIPLE PHOSPHATE CRYSTAL. COLORLESS CRYSTALS CHARACTERIZED BY STRONG

BIREFRINGENCE AND ENVELOPE OR COFFIN FORMS. PH 7.5, UNSTAINED, X400.


BILIRUBIN CRYSTALS. REDDISH BROWN, SHORT NEEDLE-SHAPED CRYSTALS

FORMING BUNDLES. PH 6.5, UNSTAINED, X400.


CHOLESTEROL CRYSTALS. COLORLESS FLAT PLATES WITH NOTCHED EDGES. A CASE OF

CYSTIC NEPHROPATHY. UNSTAINED.


CYSTINE CRYSTALS. HEXAGONAL PLATES. PH 5.5, UNSTAINED, X400.


AMMONIUM BIURATE CRYSTALS. NOTICE THE “THORNY APPLE” APPEARANCE, X400.


TYROSINE CRYSTALS. YELLOW COLOR SUGGESTS LIVER DISEASE. X400.


SULFA CRYSTALS. ALSO SEEN ARE WBC’S AND BACTERIA SUGGESTING A UTI. X400.


AMPICILLIN CRYSTALS. BUNDLES OF CRYSTALS ARE SEEN AFTER

REFRIGERATION. X400.


STARCH GRANULES. NOTICE THE REFRACTILITY. X400.


NORMAL SPERMATOZOA. X400.


TRANSITIONAL CELL CARCINOMA CELLS. THE CELLS APPEAR IN MASSES AND ARE

MULTI-LAYERED. UNSTAINED, X400.


TRANSITIONAL CELL CARCINOMA CELLS. HIGH N/C RATIO, PROMINENT

HYPERCHROMASIA AND ANISOKARYOSIS ARE SHOWN. A CASE OF URINARY BLADDER

CA. STERNHEIMER STAIN. X400.


ADENOCARCINOMA CELLS. HIGH N/C RATIO, ENLARGED NUCLEOLI AND ANISOKARYOSIS

ARE SHOWN. A CASE OF MAMMARY CA WITH METASTASIS TO KIDNEY, STERNHEIMER

STAIN, X400.


SQUAMOUS CELL CARCINOMA CELLS. STRANGE SHAPES, NUCLEAR ENLARGEMENT,

HYPERCHROMASIA AND ANISOKARYOSIS ARE SHOWN. A CASE OF CONTAMINATED URINE WITH SQUAMOUS CELL CARCINOMA OF THE UTERINE

CERVIX. STERNHEIMER STAIN, X400.

THANK YOU!!!THANK YOU!!!

URINE SEDIMENTS DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSIS FATIMA COLLEGE OF MEDICINE.

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