M ODULE 93.M EDICAL V IROLOGY Dr. Kaveh Haratian Assistant professor Department of Microbiology and immunology . Faculty of Medicine Alborz University of Medical Sciences 1
MODULE 93.MEDICAL VIROLOGY
Dr. Kaveh Haratian
Assistant professor
Department of Microbiology and immunology.
Faculty of Medicine
Alborz University of Medical Sciences
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ADENOVIRUSES
Why so called?
First isolated from human adenoids.
They cause disease of :
Respiratory tract
Eyes
Digestive tract
Urinary tract
They give rise to an estimated :
5-10% of respiratory viral infections.
Main achievement of their study :
Discovery of mRNA splicing.3
PROPERTIES OF THE VIRUS
Icosahedral, non-enveloped, DNA viruses .
80 nm in diameter .
Isolated only from vertebrates .
Recognized genera :
Mastadenovirus : from mammals(humans)
Aviadenivirus : from birds
Atadenovirus : from birds, mammals , reptiles and
a marsupial
Siadenovirus : from reptiles and birds
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PROPERTIES OF THE VIRUS
51 serotypes of human adenoviruses are now
known ;fall into groups A – F .
Disease Predominant
serotypes
Infantile gastroenteritis 40 , 41
Upper respiratory tract infections 1,2,3,5,7,11
Lower respiratory tract infections 3,4,7,21
Pharyngoconjunctival fever 3,4,7
Epidemic keratoconjunctivitis 8,9,37
Acute haemorrhagic conjunctivitis 11
Acute haemorrhagic cyctitis 7,11,21,35
Genital ulcers, urethritis 2,19,37
Gastroenteritis and pneumonia 40, 41
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REMEMBER SOME AVIAN
The only reason for that is , they may
inadvertently found in eggs used for viral
vaccine production :
CELO : chick embryo lethal orphan virus
May cause cancer in animals
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MORPHOLOGY
Capsid formed from 252 capsomers,Icosahedral
Fiber projecting from each of 12 vertices.
Pentons and Hexons
Linear dsDNA, 36-38kbp(Baltimore class 1)
Inverted terminal repeats
A protein primer at each 5` terminus.
5 early transcription units(E1A,E1B,E2-E4)
3 delayed early(IX, IVa2, E2 late)
1 late that results to L1 – L5 mRNAs.
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REPLICATION CYCLE
Divided into 2 phases
Binding to cell via fibers
Main receptor is CAR(Coxackievirus B and
Adenovirus receptor).
Binding the penton base to integrins on the cell
surface.
Fiberless virions entry by endocytosis.
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CLINICAL AND PATHOLOGICAL ASPECTS
Endemic respiratory infections in children
Most children, early in life
Stuffy nose, cough
Older children : pharyngitis
Mainly type 2
In colder area: type 3, 7
Pneumonia in younger than 2 years
Mainly transmit via fecal – oral(cups , utensils)
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CLINICAL AND PATHOLOGICAL ASPECTS
Epidemic disease in military recruits
Upper and lower respiratory tracts
Type 4, 7
Because of crowding and stress
Lasts about 10 days
Pneumonitis is common.
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CLINICAL AND PATHOLOGICAL ASPECTS
Pharyngoconjunctival fever
Pharyngitis and conjunctivitis
Mainly in children and young adults
May associated with swimming pool(after 1
week)
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CLINICAL AND PATHOLOGICAL ASPECTS
Epidemic keratoconjunctivitis (shipyard
eye)
Type 8
Permanent corneal scars and impaired sight
Transmitted by medical staff using infected
solution or inadequately sterilized instruments:
tonometer
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CLINICAL AND PATHOLOGICAL ASPECTS
Gastrointestinal infections
Mainly in infants
Types 40, 41
Don’t grow in cell culture
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CLINICAL AND PATHOLOGICAL ASPECTS
Other syndroms
Intussusception in infants
Necrotizing enterocolitis
Haemorrhagic cystitis
Meningoencephalitis
Immunosuppressed patients(AIDS,
transplantation) :
Pneumonitis
Encephalitis
Fulminant hepatitis
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CORONAVIRUSES
Infects human, birds, other animals
Mainly affects :
Ciliary epithelium of trachea
Nasal mucosa
Alveolar cells of lungs
Severe Acute Respiratory Syndrome(SARS)
Middle-East Respiratory Syndrome(MERS)
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PROPERTIES OF THE CORONAVIRUSES
Order : Nidovirals, Family: Coronaviridae
3 genera :
Coronavirus
Torovirus
Arterivirus
3 serotypes of human Coronaviruses
15 serotypes of birds and animals
Pleomorphic , 100 – 160 nm
Club-shaped glycoprotein surface spikes(20 nm) :
Crown
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PROPERTIES OF THE CORONAVIRUSES
Helical nucleocapsid
3 envelope proteins:
Matrix(gp)
Protein S : spikes, neutralizan, receptor binding,
membrane fusion, HA activity
HE: role in virus exit(neuraminidase)
Genome : largest of all RNA viruses of human.
ssRNA+, 27-30 kb, infectious
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CLINICAL AND PATHOLOGICAL ASPECTS
2-10% of common cold(OC43, 229E)
Wheezing in asthmatic and chronic bronchitis in
adults
Incubation period : 3 days
Followed by :
Nasal discharge and malaise
Excretion of virus (1 week)
Little / no fever
Cough and sore throat is not common
Toroviruses : in diarrhea samples, cause
gastroenteritis 25
EPIDEMIOLOGY AND PATHOGENESIS
Occur in colder months
Re-infection is common
Poor immune response
Antigenic mutations
Epithelium in the upper respiratory tract
Inflammation , edema, exudation (for several
days)
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RHINOVIRUSES
Family : Picornaviridae
Small, non-enveloped, ssRNA+, 18-30nm
Icosahedral symmetry
More the 150 serotypes
Labil to acid conditions
Low temperature for growth(33 ◦C)
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CLINICAL AND EPIDEMIOLOGICAL
ASPECTS
Upper respiratory tracts
Spread by aerosol/hand contact/surface of cups
and plates
Incubation period: 2-3 days
Nasal congestion, sneezing, sore throat,
headache, cough
Fever is rare
Serious sequel in chronic bronchitis and
asthmatics
Children are more susceptible than adults.
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