Protocol #11 2ACh10 ug/kg decrease in heart rate (bpm) Decrease
in heart rate (bpm)Baseline 110 108.3Artery 8.! 8".#$ein 8#.
83.3Protocol #2 No Atropine Estimated [Atropine] (M)0 1ACh (ug/kg)
Decrease in heart rate (bpm)0 (Baseline) 00.1 10.30.! 1.80." 33.11
#%.%! &.8" 8&.310 %".#!0 %&."0 %&.100
%&.Atropine Estimated [Atropine] (M)2 mg/kg 0.0001# 1ACh
(ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 !0.! #0." 1!1
1%! 3!."" "310 &!!0 8""0 %3100 %3Atropine Estimated [Atropine]
(M)5 mg/kg 0.0003" 1ACh (ug/kg) Decrease in heart rate (bpm)0
(Baseline) 00.1 .!0.! &.30." 13.!1 !0.3! 3!.8" ".10 &.!!0
8%.&"0 %.!100 %.!Atropine Estimated [Atropine] (M)10 mg/kg
0.000% 1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1
10.! 1.0." &.31 11."! 1&.&" !.110 #0.&!0 "".!"0
".&100 %.8Atropine Estimated [Atropine] (M)20 mg/kg 0.00138
1ACh (ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 1.80.!
!."0." #.11 .8! 1!."" !0.810 33.3!0 #&.%"0 8.&100
&"Protocol #3No Tubocurarine1ACh (ug/kg) Decrease in heart rate
(bpm)0 (Baseline) 00.1 &.80.! 1#.10." !%.%1 #&.!! ".""
8".#10 %#.#!0 %&"0 %&100 %&Tubocurarine2 mg/kg 1ACh
(ug/kg) Decrease in heart rate (bpm)0 (Baseline) 00.1 100.!
1&.%0." 33.11 #%.3! 8.&" 8%.&10 %%.!!0 101.3"0 101.3100
101.3Protocol #4No Neostigmine1ACh (ug/kg) Decrease in heart rate
(bpm)0 (Baseline) 00.1 #.!0.! ".80." 1#.11 !#.! 3%.8" #10
&%.!!0 8&"0 %0.!100 8%.1Neostigmine0.1 mg/kg 1ACh (ug/kg)
Decrease in heart rate (bpm)0 (Baseline) 00.1 1."0.! 1."0." ".!1
18.8! !8.8" ""10 "."!0 &0.8"0 &0.!100 &0.83Decrease in
heart rate (bpm)108.883.%83.%2 3Decrease in heart rate (bpm)
Decrease in heart rate (bpm)0 0#.1 ".!10.# %.%!0.8 !1.83".#
!%.&"! "!.3&" .183.3 83.#8&." 88&." 88&." 82
3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0 0! !.#
#10.& 101%.3 1&3!.1 !%"".& "!&!.% 883. 8!8".&
8!8&.% 812 3Decrease in heart rate (bpm) Decrease in heart rate
(bpm)0 0! 1."! 3.1.! .!10.# 11.%18.& 1%.!3".# 3".&"0 "!.3.
&.3&" &.1&" &.12 3Decrease in heart rate (bpm)
Decrease in heart rate (bpm)0 0# 0" 0.! !.18.3 .#1!." 8."!&.1
1%.!3%. 3#.!".! #%.!&0.8 8."&" &!.82 3Decrease in heart
rate (bpm) Decrease in heart rate (bpm)0 0! 01 0! 03 3 "11 818 1030
18#" 3""3 #12 3Decrease in heart rate (bpm) Decrease in heart rate
(bpm)0 0#.1 #.&10.# &.81. 1&.1#1. !8".! #".1&%.1
8.%%3.& 8#.3100 %1."100 %!.!100 %!.!2 3Decrease in heart rate
(bpm) Decrease in heart rate (bpm)0 0#.1 .!10.# %.3!!.% 18.3&."
31.1&0.8 #8.!&& &3.8%." 88.1%".8 %.%%&.% %.%100
%.%2 3Decrease in heart rate (bpm) Decrease in heart rate (bpm)0
0#.! #.&.3 8.318.8 1.1!%.! !8#8 ##.&0.% 8.%8&." 8!.%8%.
%0.&%1.& %!.!%3.8 %!.!2 3Decrease in heart rate (bpm)
Decrease in heart rate (bpm)0 0#.! 0."8.# .&1#. 13."!!.%
!.%3".# #0.%0.# "8!." %.#!." &3!." &3#. &3Decrease in
heart rate (bpm)Averae !td Dev n !td Error Acet"lcholine into
Arter"8".1& 1.1&18"%! 3 0.!#%83.%333333333 0."0#0&0%% 3
0.3&"#&Decrease in heart rate (bpm)Averae !td Dev n !td
Error0 0 3 0."333333333 3.3080&0%38# 3 1.%0%%1"1!.3&
3.8#&"1001&3 3 !.!!130%!".!333333333 .8310""313 3
3.%#3%1!338.3333333333 10.#1#"&31!3 3 .01!8""&.3&
%.03&&&11" 3 ".!1&38""&8.8 &.3%"&!&
3 #.!"&%3388&.3& .%"&!"0#1#& 3
#.01&&0#%0.3& .308%88!%&1 3 3.#!#%1%0.3&
.308%88!%&1 3 3.#!#%1%0.3& .308%88!%&1 3
3.#!#%1Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0 3
0! 0 3 0#.8 1.38"#0#1 3 0.810.% 1.01#88%1"" 3 0."8"%#"18.#333333333
1.!"0333!88% 3 0.&!1880331.! 1.%1"&!##01 3 1.100##"3."&
1.%13%83!%3 3 1.10"03%&0.%& !.083!00!13 3
1."0"%1#383."333333333 1."01110%%% 3 0.8&8.%
"."%&3!0&8&& 3 3.!311#&8&.3
.0!!#"&%& 3 3.#&&0&&Decrease in heart rate
(bpm)Averae !td Dev n !td Error0 0 3 03.!333333333 !."813#331%" 3
1.#%033%3#.1333333333 !.&%&0!!!!1 3 1.1#81"8."333333333
#.0#1#"188#3 3 !.33333331#.! ".33"&!8!88 3 3.080"8##!3."&
8.000!08330 3 #.18%!!##!."& 1!.1"#1#8811 3
&.01&!011"%." 1#."08!&3"01& 3
8.3&3""."333333333 13.13%3810103 3 &."80!"!8!.#333333333
11.%3#%!##8 3 .8%0"3%8!.#333333333 11.%3#%!##8 3 .8%0"3%Decrease in
heart rate (bpm)Averae !td Dev n !td Error0 0 3 01.& !.081"%%%"
3 1.!018"0#!.! !.""3#!%0& 3 1.#!!3".! !.#3&%!1! 3
1."8!1%!8.&333333333 !.""3 3 1.#881011!.% #.130!"03&% 3
!.3331!!#.1333333333 #.301""0108! 3 !.#83"01138.1&
3.#&8%8#"!! 3 !.008"%!&"3."333333333 3.&8"%388%&! 3
!.18"81!88.3333333333 !.""#081&00 3 1.#"%%8&!."333333333
!.10!3!01 3 1."0&0!0Decrease in heart rate (bpm)Averae !td Dev
n !td Error0 0 3 01.!& 1.101"1#10%" 3 0.3"%"%"1.1&
1.!"830"&3%! 3 0.&!#83!!.0333333333 !.0"0!03!#! 3
1.1838"##.!& !.1%3%310!!% 3 1.!&&.8333333333
#.0&!!3%0& 3 !.3"11!!&13.!& .%#!&1! 3
3.8#%#!1!0.#333333333 11.83%0"%#&3 3 .83"!8#331.%&
1".0#&0"0"& 3 8.8&!1%!#%."& 1&.30&8%800#" 3
%.%%!&1%".3333333333 1&.!#33"!08" 3 %.%""#"3Decrease in
heart rate (bpm)Averae !td Dev n !td Error0 0 3 0"."333333333
1.%8"&8!8011 3 1.1##%!!10.&& 3.1"%#83#" 3
1.8!&8&0!1.! &."38"&"03! 3 #.3"!3%#38.%333333333
%.8&38&1!#3" 3 ".&008!!"". 10.!13!!&183 3
".8%0%!&&.8 8.3!#383"3 3 #.80&!81%0.8 ".#003"#0% 3
3.!"!&%.1& #.31083%0"!1 3 !.#888#1%.# 3.%3##311# 3
!.!&1"33%.# 3.%3##311#Decrease in heart rate (bpm)Averae !td
Dev n !td Error0 0 3 0.&& !.%%0"#0#!3 3
1.&!"8%#1!."333333333 #.800%%&1# 3 !.&0!0"8!#.8&
&.##&3&0%00& 3 #.!%%3%.3 %.!3!""11101 3
".330#1"%!."& 1!.#81!"33# 3 &.!088880.1 8.#8"8&00%# 3
#.8%%31%&%!.!& .0#"108!1"! 3 3.#%01##%%8 !.%103!##!! 3
1.80!&&8%8.& !.30"1!"18% 3 1.331"%%.# !.!0"30%111 3
1.30"1181Decrease in heart rate (bpm)Averae !td Dev n !td Error0 0
3 0#.3& 0.!88&"13# 3 0.1&.8 1.3!!8&"""" 3
0.&3&!1.3333333333 !.3"8&1%#!& 3
1.31&&%%!&.!& !.380&08%1 3
1.3&&"%8##.1333333333 #.11%8&0"#81 3
!.3&808#&.%333333333 3.""011&3% 3 !.0#%1!83.!
#.1"81!#"&&3 3 !.#00%#38%.1 1.% 3 1.0%%""%1.3&
1.0#083!%%%& 3 0.00%!"!%1.& !.38%"0!%1 3
1.3&%13"Decrease in heart rate (bpm)Averae !td Dev n !td Error0
0 3 0!.0& 1.%13%83!%3 3 1.10"03%"."333333333 3."%#%038003 3
!.0&""18&11.1 ".13%003 3 !.%&0#1!!!.8&
#.0"010!8&%# 3 !.3383!83".0333333333 .0"83!&0! 3
3.#%&&&&1"&.8 !.&0""#%8"1& 3
1."!0#%%".8 3.#"%&8&&8# 3 1.%%%8#8.&& "."3#8!%1
3 3.1%&0#&!8."& ".#3&!1!" 3 3.13%1&8%%.#&
#.3""838%%3 3 !."1#8##8Acet"lcholine into Arter"
8".1&Acetylch'line int' 83.%3333Carbach'l int' Art
8%.3Carbach'l int' (e %0.&0 0 0 0 0 00.1 ."33333 ! 3.!33333
1.& 1.!&0.! 1!.3& #.8 #.133333 !.! 1.1&0." !".!3333
10.% 8."33333 ".! !.0333331 38.33333 18.#3333 1#.! 8.&33333
#.!&! "&.3& 31.! !3."& 1!.% &.833333" &8.8
"3."& #!."& !#.13333 13.!&10 8&.3&
&0.%& "%." 38.1& !0.#3333!0 %0.3& 83."3333 ."3333
"3."3333 31.%&"0 %0.3& 8.% 8!.#3333 8.33333 #%."&100
%0.3& 8&.3 8!.#3333 &!."3333 ".3333380828486889092Efect
on Heart Rate from Chemical Injection into Cat ModelChemical
Injected and WhereDecrease in Heart Rate (bpm)0 0 0 0 00.1 "."33333
.&& 10!.8& 10".1&0.! 10.&& 1!."3333
%&.#3333 %%.#33330." !1.! !#.8& 8#." 8&.!1 38.%3333
3%.3 %."& &1.!! "". !."& "1."& "!.%" &&.8
80.1 !%."3333 30."10 %0.8 %!.!& !0.03333 18.33330 10 20 30 40
50 60 70 80 90 1000102030405060708090100Atropine on Heart Rate
Response to Acetylcholine0.0 mg/kg Atropine0.5 mg/kg Atropine1
mg/kg Atropine2 mg/kg Atropine5 mg/kg AtropineAcetylcholine
(!"#!)Decrease in Heart Rate (bpm)0 20 40 60 80 100
120020406080100120$bocrarine on Heart Rate Response With
Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and 5
mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg
T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate
(bpm)80828486889092Efect on Heart Rate from Chemical Injection into
Cat ModelChemical Injected and WhereDecrease in Heart Rate (bpm)!0
%.1& %8 1.& 1#.!3333"0 %.# %8.& 1&.&&
1#.%3333100 %.# %%.# 1%.&& 1&.&33330 0 0 0 00.1
#.3& !.0& 10!.1& 81.&0.! .8 "."33333 %#.&
&8.!33330." 1.33333 11.1 80.%3333 &1.#1 !&.!&
!!.8& ".% 0.#3333! ##.13333 3".03333 #8.0& #.&&"
&.%3333 "&.8 !.0& !8.%333310 83.! ".8 1&.%
1%.33333!0 8%.1 8.&& 1".!& 1&.!3333"0 %1.3&
8."& !!.83333 !1.#100 %1.& %.#& 31.3333 30.%&0 20
40 60 80 100 120020406080100120$bocrarine on Heart Rate Response
With Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine and
5 mg/kg T!ocrarine"ar!achol Alone"ar!achol and 5 mg/kg
T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in Hearth Rate
(bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate
Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine
and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate
(bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate
Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine
and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate
(bpm)decrease in heart rate (bpm)80828486889092Efect on Heart Rate
from Chemical Injection into Cat ModelChemical Injected and
WhereDecrease in Heart Rate (bpm)0 10 20 30 40 50 60 70 80 90
1000102030405060708090100Atropine on Heart Rate Response to
Acetylcholine0.0 mg/kg Atropine0.5 mg/kg Atropine1 mg/kg Atropine2
mg/kg Atropine5 mg/kg AtropineAcetylcholine (!"#!)Decrease in Heart
Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on Heart
Rate Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol
and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in
Hearth Rate (bpm)80828486889092Efect on Heart Rate from Chemical
Injection into Cat ModelChemical Injected and WhereDecrease in
Heart Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on
Heart Rate Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol
and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in
Hearth Rate (bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on
Hearth Rate Response With Acetylcholine or
CarbacholAcetylcholineAcetylcholine and 5 mg/kg
#eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate
(bpm)0 20 40 60 80 100 120020406080100120%eosti!mine on Hearth Rate
Response With Acetylcholine or CarbacholAcetylcholineAcetylcholine
and 5 mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Decrease in Heart Rate
(bpm)Tubocurarine0 20 40 60 80 100 120020406080100120$bocrarine on
Heart Rate Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol
and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in
Hearth Rate (bpm)0 20 40 60 80 100 120020406080100120$bocrarine on
Heart Rate Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and 5 mg/kg T!ocrarine"ar!achol Alone"ar!achol
and 5 mg/kg T!ocrarineAcetylcholine"Carbachol (!"#!)Decrease in
Hearth Rate (bpm)Protocol #11 2ACh10 ug/kg increase in tensi'n (g)
increase in tensi'n (g)Baseline 0 0Artery 0.& 0.#$ein 0
0Protocol #2 No tubocurarine1ACh (ug/kg) increase in tensi'n (g)0
(Baseline) 0.00.1 !.%0.! #.30." &.%1 1#.3! !".0" #".010 1.#!0
&&.1"0 8#.3100 8%.3tubocurarine0.5 mg/kg 1ACh (ug/kg)
increase in tensi'n (g)0 (Baseline) 00.1 00.! #."0." 31 .1! 8.3"
!!10 3.#!0 "0"0 &.#100 &3."tubocurarine1 mg/kg 1ACh (ug/kg)
increase in tensi'n (g)0 (Baseline) 00.1 0.&0.! 1.80." !.81
#.3! &.8" 18.110 3".!!0 3%.""0 "%.1100 .%tubocurarine2 mg/kg
1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 1."0.! 1."0."
3.11 %.!! 1#." !.%10 ##.!0 "%.!"0 &0.8100 &!.3tubocurarine5
mg/kg 1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 00.1 00.!
00." 11 #.!! !.8" #.!10 %!0 1#."0 !%.!100 #1.&Protocol #3No
Atropine1ACh (ug/kg) increase in tensi'n (g)0 (Baseline) 0.00.1
!."0.! #.0." %.31 1.0! !8."" #%.810 ".8!0 &%.#"0 8#.3100
88.8Atropine )h'uld ha$e n' e**ect5 mg/kg 1ACh (ug/kg) increase in
tensi'n (g)0 (Baseline) 00.1 !.80.! #.30." 8.%1 1.&! !8.""
"0.!10 .!!0 &%.#"0 8&."100 88.Protocol #4No Neostigmine1ACh
(ug/kg) increase in tensi'n (g)0 (Baseline) 0.00.1 !.80.! ".30."
10.31 1%.! 33.1" "".!10 &0."!0 8!.%"0 8%.&100
%0.0Neostigmine0.1 mg/kg 1ACh (ug/kg) increase in tensi'n (g)0
(Baseline) 0.00.1 .00.! 10.&0." !0.1 3%.1! "3.#" &0.810
8!.!0 8#.3"0 8&."100 8%.03increase in tensi'n (g)00.%02
3increase in tensi'n (g) increase in tensi'n (g)0 0!.1 1.13."
3.#&. ".&13.% 13.8!" !".3##.# #!."1.8 !.1&".&
&".%&%.! 83.%8.8 8".12 3increase in tensi'n (g) increase in
tensi'n (g)0 00 1.#1.# 1.#3." !.8&. #.%10.# 10.#!!.! !!.%3".#
38.!"!.1 "0.&%.# 8.1.3 &0.82 3increase in tensi'n (g)
increase in tensi'n (g)0 01.# 1.&1. 1.#!.1 !.1#.% 3.8.!
&.31".3 1#.!!.# !8.###.# 38.#".! ".18.1 .12 3increase in
tensi'n (g) increase in tensi'n (g)0.0 00.0 0.&0.0 !.1!.1
!.8!.8 3."%.& &.#1!." %.&1&.# 1&.#!".&
!8."#3.1 #".8"#.! "!.82 3increase in tensi'n (g) increase in
tensi'n (g)0 00 00.& 00.& !.13." 3."!.8 ".%%.&
1.&10.# !!.%13.! !3.!" "#3".3 0.#2 3increase in tensi'n (g)
increase in tensi'n (g)0 0!.1 !.13." 3.".% &.313.% 1#.!!#.3
!#.%##.# ##.31.8 1.&".0# &".18".# 8.!88.% 88.%2 3increase
in tensi'n (g) increase in tensi'n (g)0 0!.1 !.13." 3.8&.
&.313.% 13.8!#.3 !3.%##.# ##.31.1 !.3&".& &."8".#
8&.%%0.3 8%.32 3increase in tensi'n (g) increase in tensi'n
(g)0 01.# 1.&3." !.#.% .!11.8 11.1!1." 1%#0.3 3&.&"%.3
"3.&!.% &0.%8#.& 8388.% 8.%2 3increase in tensi'n (g)
increase in tensi'n (g)0 03." 3.1.! "."13.% 11.1!1." !3."33.3
3#.3"0.& "3.3&!.% ".#&%.! &&.!81.3 81.&8"
8!#ncrease in tension ()Averae !td Dev n !td Error00.&
0.!"111#&8 3 0.1#"!%0 0 3 0#ncrease in tension ()Averae !td Dev
n !td Error0 0 3 0!.0333333333 0.%018#%%"0 3
0."!08333.&333333333 0.#%3!88!8! 3 0.!8#8001&.0&
1.1%303"3##" 3 0.88&%%31# 0.!#"&"1311 3 0.1"!&"!"!".1
0.1&3!0"0808 3 0.1#3.%& 1.30"11813 3
0.&"3"1031.&& 0.3"1188#"8# 3
0.!0!&"88&.!333333333 0.&"&18&&&%# 3
0.#3&1!8!.#& !.830&&10!% 3 1.3%%8&.0&
!.11!0!"03 3 1.!1%"#ncrease in tension ()Averae !td Dev n !td
Error0 0 3 00.#& 0.808!%03&% 3 0.#&!.#333333333
1.&8%&8"83#" 3 1.03333333.1 0.30"""1!&" 3 0.!081.!
1.3"!&%!"8 3 0.&810!"%.& 1.!1!#3"""3 3 0.&!!.3&
0.#&!"81"! 3 0.!&!8#"13.& 1.#18%1%&&% 3
0.81%!13&"0.%333333333 1.0%!&!! 3 0.1&3#!8.3
1.01#88%1"" 3 0."8"%#"&!.8& 1.833%3%!%38 3
1.0"88!"3#ncrease in tension ()Averae !td Dev n !td Error0 0 3
01.!& 0."13101#3% 3 0.!%!&311. 0.! 3
0.11"#&01!.3333333333 0.#0#1#"188# 3 0.!333333#.3333333333
0.""0&"&0"#& 3 0.31&%&%&&.1
0.818"3"!&&! 3 0.#&!"811".8& !.01080#1"0% 3
1.10%38330 #.130!"03&% 3 !.3331!#0.&& 3.1%#!"%"" 3
1.8##!10!"&.1333333333 1.&03%1&0""% 3
0.%83&"&&.0333333333 1.00##"%1# 3 0."8118"#ncrease in
tension ()Averae !td Dev n !td Error0 0 3 00.&333333333
0.&"0"""3#%% 3 0.#3333331.! 1.081"38! 3 0.!##%%8!.&
0."13101#3% 3 0.!%!&31".1& 3."10#0""8 3
!.0!&"310."& 3.&"0&8 3 !.1!31"!81.3&
%.!!8%3%%8%& 3 ".3!8331!.#& 1".&03%!&3!! 3
%.0&3&.8 18."8"&!! 3 10.&30#8"3.!333333333
1".!&!%08"8 3 8.81&8#81"%.&&
10.8&&33"&83 3 .!&%8"1#ncrease in tension ()Averae
!td Dev n !td Error0 0 3 00 0 3 00.!333333333 0.#0#1#"188# 3
0.!3333331.!& 0.&3&111#&% 3
0.#!""&1"3.&333333333 0.#0#1#"188# 3 0.!3333333.8333333333
1.&8%&8"83#" 3 1.033333310.! .!#%8!0#31 3 3.1&08%11#.1
&."3103%#"" 3 #.#18"!11&.1333333333 ".#38&"&3% 3
3.!"8#%3!3.0& 1".&!0""810& 3 %.0#8!"#".8
13.0#!!!#3"& 3 &."3011#ncrease in tension ()Averae !td Dev
n !td Error0 0 3 0 0!.!333333333 0.!30%#010&& 3 0.1333333
0.13.8& 0.3"08"!%1 3 0.3& 0.!&.8333333333 1.!8"8!0101"
3 0.!38 0."1#.& 1.13"&81%! 3 0.""% 1!".% !.!&1"33383 3
1.311#8&& !#.1& 3.1#%"!01# 3 1.818% "3.0& !.3%!#%!
3 1.3&88" 10&."133333333 !."0010## 3 1.##3#3&3 !08".3
0.%"3%3%!01# 3 0.""0&"&1 "088.8& 0.0"&&3"0!%
100#ncrease in tension ()Averae !td Dev n !td Error0 0 3
0!.3333333333 0.#0#1#"188# 3 0.!3333333.8& 0.#0#1#"188# 3
0.!333333&.%333333333 0.8"0#%00"#8 3 0.#%1030&1#.8
1.#!0&&33 3 0.%"0#38"!"."& !."#8!01%80" 3
1.#&1!0"1#.3 3.3&&8%1"08 3 1.%"0!13&3.! !.#"83!"! 3
1."3%#80#&&.! 1.%#&%!!33# 3 1.1!3%818.%333333333
1.3#!88!#&1% 3 0.&&"3138%.# 0.8"##003" 3
0.#%3!883#ncrease in tension ()Averae !td Dev n !td Error0 0 3
01.%& 0.&3&111#&% 3 0.#!""&1"3.&333333333
1.##01!&"0# 3 0.8#"!#8!&.8 !.1%31&1!1%% 3
1.!!!81#.1& #.&18#038#% 3 !.&!#1&1!#."333333333
&."!3"18803 3 #.3#3&0""##.# %.##!%881" 3
".#"1%111.1333333333 8."%&8&%"!8 3 #.%3%81#&"."&
.#!%100"0&3 3 3.&118#!%8".8 3.#8!81#%"3" 3 !.01080#!88.
1."&1!33# 3 0.%0&3&&!1.83%3&1.0!11#%#ncrease in
tension ()Averae !td Dev n !td Error0 0 3 0#.! 1."&1!33# 3
0.%0&3&&!&.#& !.8!1%3&88# 3 1.!%!#1".!
#.881"%80%% 3 !.8183%!!8.0333333333 %.30#3##81 3
"."33&!3#0.3333333333 11.3!&1013!3 3
."3%&0&8"8.!& 10.%31&"801 3
.311#"3#&3.333333333 8.!3#1&&!%3 3
#.%&8&3!"80.!333333333 3.10"313% 3 !.113&11883."
3.#%8&031# 3 !.0033308".3333333333 3."1188#"8#3 3
!.0!&"8&"!.1&3#"!1.!"131&3Base 0Acetylch'li
0.&Acetylch'li 0Carbach'l i &0.0Carbach'l i 0.00 0 0 0 0
00.1 !.033333 0.#& 1.!& 0.&33333 00.! 3.&33333
!.#33333 1. 1.! 0.!333330." &.0& 3.1 !.333333 !.&
1.!&1 1# .! #.333333 ".1& 3.&33333! !".1 %.&
&.1 10."& 3.833333" #3.%& !!.3& 1".8& 1.3&
10.!10 1.&& 3.& 30 !.#& 1#.1!0 &.!3333 "0.%3333
#0.&& 3&.8 1&.13333"0 8!.#& 8.3 "&.13333
"3.!3333 3.0&100 8&.0& &!.8& &.03333
"%.&& #".80 0 0 0!.!33333 !.333333 0.810313
!.######3.8& 3.8& 1.##"0!8 3.#""""&.833333 &.%33333
!.8%"!&3 .0#####1#.& 1#.8 ".3#"!1 10.3333!".% !"."&
%."!38"# 1&.%3333#.1& #.3 1&."1"" 31.1"""0 10 20 30 40
50 60 70 80 90 1000102030405060708090$bocrarine on eletal Mscle
Response to Acetylcholine0.0 mg/kg T!ocrarine0.5 mg/kg T!ocrarine1
mg/kg T!ocrarine2 mg/kg T!ocrarine5 mg/kg T!ocrarineAcetylcholine
(!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90
1000102030405060708090Atropine on eletal Mscle Response With
Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine
and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg
AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)3.0& 3.! !#.&!308 #!.""""&."1333 &&.!
3!."133& "1."3&&88".3 8.%3333 #".#"131
"8.1&88.8& 8%.# !.%8"%1 "%.!"""acetylch'line by itsel*and
in the presence '* atr'pine and e**ect '* carbach'l by itsel* and
in the presence '*atr'pineAC+, AC+ -e' car!acho0.1 neo$tig0 0 0 0
00.1 1.%& #.! 4.2 4.80.! 3.&33333 &.#& 8.4 8.60."
&.8 1".! 17.3 17.41 1#.1& !8.03333 28.8 29.2! !#."3333
#0.33333 43.3 44.6" ##.# "8.!& 65.1 60.110 1.13333 &3.3333
72.1 72.7!0 &"."& 80.!3333 79.3 78.4"0 8".8 83." 81.0
81.1100 88. 8".33333 83.8 80.40 10 20 30 40 50 60 70 80 90
1000102030405060708090%eosti!mine on eletal Mscle Response With
Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5
mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0
10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal
Mscle Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5
mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine
on eletal Mscle Response With Acetylcholine or
CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg
#eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)Acetylcholine into Artery "ar!achol into
Artery01020304050607080Efect on eletal Mscle from Chemical
Injection into Cat ModelChemical Injected and WhereIncrease in
$ention (!)0 10 20 30 40 50 60 70 80 90
1000102030405060708090$bocrarine on eletal Mscle Response to
Acetylcholine0.0 mg/kg T!ocrarine0.5 mg/kg T!ocrarine1 mg/kg
T!ocrarine2 mg/kg T!ocrarine5 mg/kg T!ocrarineAcetylcholine
(!"#!)Increase in $ension (!)0 10 20 30 40 50 60 70 80 90
1000102030405060708090Atropine on eletal Mscle Response With
Acetylcholine or CarbacholAcetylcholine AloneAcetylcholine
and5mg/kg Atropine"ar!achol Alone"ar!achol and 5 mg/kg
AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)Acetylcholine into Artery "ar!achol into
Artery01020304050607080Efect on eletal Mscle from Chemical
Injection into Cat ModelChemical Injected and WhereIncrease in
$ention (!)0 10 20 30 40 50 60 70 80 90
1000102030405060708090%eosti!mine on eletal Mscle Response With
Acetylcholine or CarbacholAcetylcholine AloneAcetlycholine and 5
mg/kg #eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0
10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal
Mscle Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5
mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine
on eletal Mscle Response With Acetylcholine or
CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg
#eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0
10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal
Mscle Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5
mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine
on eletal Mscle Response With Acetylcholine or
CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg
#eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension (!)0
10 20 30 40 50 60 70 80 90 1000102030405060708090Atropine on eletal
Mscle Response With Acetylcholine or CarbacholAcetylcholine
AloneAcetylcholine and5mg/kg Atropine"ar!achol Alone"ar!achol and 5
mg/kg AtropineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)0 10 20 30 40 50 60 70 80 90 1000102030405060708090%eosti!mine
on eletal Mscle Response With Acetylcholine or
CarbacholAcetylcholine AloneAcetlycholine and 5 mg/kg
#eo$tigmine"ar!achol Alone"ar!achol and 5 mg/kg
#eo$tigmineAcetylcholine"Carbachol (!"#!)Increase in $ension
(!)