UNIVERSITY OF WISCONSIN- · Web viewUNIVERSITY OF WISCONSIN-MADISON SPECIFICATION UW-MDN-06-B2-2015 GENERAL PURPOSE CLEAN AIR BIOLOGICAL SAFETY CABINET CLASS II, TYPE B2 – 100%...

UW-MDN-06-B2-2015 PAGE: 1Clean Air and Biosafety Cabinet SpecificationsUniversity of Wisconsin-UW

UNIVERSITY OF WISCONSIN-MADISONSPECIFICATION UW-MDN-06-B2-2015

GENERAL PURPOSE CLEAN AIR BIOLOGICAL SAFETY CABINETCLASS II, TYPE B2 – 100% Exhaust-BENCH MODEL w/HARD CONNECTION

ESTIMATED AIR FLOW REQUIREMENTS

Four Foot Model: 675 cfm @ 1.9” WCSix Foot Model: 1000 cfm @ 2.3” WC

SPECIFICATION OUTLINE

1.0 SCOPE OBJECTIVE, CLASSIFICATION, INNOVATIVE DESIGN AND DEFINITIONS1.1 Scope1.2 Objective

1.2.1 Noncompliance1.3 Classification

1.3.1 Sizes1.3.2 Style

1.4 Innovative Design1.5 Definitions

2.0 APPLICABLE DOCUMENTS2.1 Specifications and Standards

International StandardsNational StandardsFederal SpecificationsFederal StandardsMilitary SpecificationsMilitary StandardsPerformance Test Methods

2.2 Other PublicationsAir Moving and Conditioning Association (AMCA)American National Standards Institute, Inc. (ANSI)American Society of Mechanical Engineers (ASME)Illuminating Engineering Society (IES)National Electrical Code (NEC)Underwriter's Laboratories (UL)United States Atomic Energy Commission (AEC)Canadian Standards Association (CSA)Institute of Environmental Sciences and Technology (IEST)

3.0 REQUIREMENTS3.1 Bid Submittal Requirements

3.1.1 Motor and Fan Graphs3.1.1.1 Motor Performance Graph(s)3.1.1.2 Fan/Motor Performance Graph(s)

3.1.2 Fabrication Drawings and Performance Envelope3.1.3 Submitted Bids3.1.4 Selected Supplier3.1.5 Field Fan/Motor Performance Test3.1.6 Field Internal Plenum Pressure Test3.1.7 Field Specification Compliance Inspection

3.2 General Description3.2.1 Complete Unit3.2.2 Independent Unit3.2.3 Window Edge Protection3.2.5 Work Area3.2.6 Work Zone Perforated Exhaust Grills3.2.7 Scavenger Slots3.2.8 Drain Spillage Trough3.2.9 Exhaust Filter Protector3.2.10 Reinforced Panels3.2.11 Construction and Fabrication Requirements

Class II B2 100% Exhaust


3.2.11.1 Strength and Rigidity Requirement3.2.11.2 Ease of Cleaning

3.2.11.2.3 Gliders3.2.11.2.3.2 Channel Stand

3.2.11.2.4 Optional Adapter Rails for Laboratory Bench3.2.11.3 Dimensional Tolerances

3.2.12 Fabrication Welding Requirements3.2.13 Services in Air Passages3.2.14 Service Control Center

3.3 Dimensions3.3.1 Overall Dimensions3.3.2 Inner Dimensions of Work Area

3.4 Assembly3.4.1 Door Way Requirement3.4.2 Bolted Cabinet Sections3.4.3 Cabinet Sash Requirements

3.4.3.3.3 Sliding Window Exhaust Slots3.4.3.3.4 Sash Handles

3.4.3.3.4.5 Sash Alarm 3.4.3.4 Unobstructed Work Zone Interior Access Requirement

3.4.4 Operational Sash Height

3.5 Materials and Components3.5.1 Corrosion and Moisture Resistant Requirement3.5.2 Sheet Metal and Finishes

3.5.2.4 Carbon Steel Finish3.5.2.4.1.1 Finish Color: White

3.5.2.4.2 Puncture and Tear Requirement3.5.2.4.3 Finish Texture Required: Flat

3.5.2.5 Supply Air Grill (SAG) and Air Curtain3.5.2.6 External Air Balancing Damper3.5.2.7 Exhaust Transition and Airflow Monitor

3.5.3 Glass3.5.4 Filters3.5.5 Gaskets, Seals and Clamps3.5.6 Fan(s)3.5.7 Electrical Components

3.5.7.1 Electrical Requirements – General3.5.7.2 Main Power 3.5.7.3 Motor3.5.7.4 Motor Controller3.5.7.7 Controls

3.5.8 Plumbing3.5.8.1 Work Area Trough Drain Pipe Assembly3.5.8.2 Aerosol Sampling Port Assembly3.5.8.3 Side Wall Hose Cock Assembly

3.5.8.3.3 Petcock Labels3.5.9 Pressure Gauges and Cabinet Operator Warning Systems3.5.10 Leak Tight Damper in Exhaust Transition

3.6 Lighting and Germicidal Radiance

3.7 Airflow3.7.1 Downflow Velocity3.7.2 Face Velocity

3.7.2.1 8 Inch Access Opening to Workspace3.7.7 Secondary Face Velocity Test

3.8 Temperature Rise3.9 Noise3.10 Vibration3.11 Leak Tight Construction3.12 Labels

3.12.1 Restricted Substances Label3.12.2 Manufacturer Label3.12.3 Decontamination Signage3.12.4 Field Certification Plate and Recertification Interval3.12.5 NSF49 Seal3.12.7 Initial Field Certification (IFC) Label



3.12.8 Ground Fault Circuit Interrupter (GFCI) Label3.12.9 Sash Location Arrows3.12.10 Operator Instruction Label

3.13 Operations Manual and Service Video3.14 Pressure Gauges, Blower Interlock, and Cabinet Operator Warning Systems3.15 Parts List and Required Parts Discount3.16 Options and Requirements3.17 Drawings

4.0 QUALITY ASSURANCE PROVISIONS4.1 Qualifications

4.1.1 Procedure for Obtaining Qualification4.1.2 Prototype Tests

4.2 Production Quality Control Tests4.2.1 Pressure Decay/Soap Bubble Test4.2.2 HEPA Filter Leak Test4.2.3 Down flow Velocity and Volume4.2.4 Inflow Velocity and Volume4.2.5 Temperature Rise4.2.6 Lighting Intensity4.2.7 Noise Level4.2.8 Vibration4.2.9 UV Intensity Test4.2.10 Electrical Leakage and Ground Circuit Resistance and Polarity4.2.11 Airflow Smoke Patterns Test

5.0 PREPARATION FOR DELIVERY5.1 Packaging5.2 Packing

5.2.5 Building Address and Room Number Label

6.0 NOTES6.1 Guarantee

6.1.1 Limited Warrantee6.2 Qualification6.3 Inspection and Certification of Cabinet Delivered FOB Site

6.3.1 Notification of Delivery6.3.1.1 Building Address & Room Number-required6.3.1.2 Direct Delivery Option to the Laboratory Room

6.3.2 Dock Inspection6.3.2.3 Extreme Needs Situation on Damaged Goods

6.3.3 Certification Tests6.3.4 Penalties for Noncompliance

6.4 Fabrication and Delivery Time6.4.1 Inspection and Certification of Cabinet Delivered FOB Laboratory

APPENDIX AGRAPH 1: Example Motor Performance Graph (Paragraph 3.1.1.1)

GRAPH 2: Example Fan Motor Performance Graph (Paragraph 3.1.1.2)

APPENDIX BFIGURE 1: Example Dimensions (Taken from Paragraph 3.3)



UNIVERSITY OF WISCONSIN-MADISONSPECIFICATION UW-MDN-06-B2-2010

GENERAL PURPOSE CLEAN AIR BIOLOGICAL SAFETY CABINETCLASS II, TYPE B2 - NEGATIVE EXTERIOR PLENUM – BENCH MODEL w HARD CONNECTION

ESTIMATED AIR FLOW REQUIREMENTS

Four Foot Model: 675-cfm @ 1.9” WCSix Foot Model: 1000-cfm @ 2.3”WC

1.0 SCOPE, OBJECTIVE, CLASSIFICATION, INNOVATIVE DESIGN, DEFINITIONS

1.1 Scope This performance and hardware specification covers biological safety cabinets designed with internal fan/motor systems in which all the airflow is discharged to the building exhaust system. Integral with the cabinet fan/motor system is a high efficiency particulate air filtration system designed with efficiency such that pathogen free air is produced in the work zone and simultaneously discharged to a building exhaust system. The use of small quantities of toxic chemicals and isotopes with known vapor pressure at room temperature are also allowed in a Class II, Type B2-negative exterior plenum-hard connection biological safety cabinet without prior approval from the University Agent or Bio-safety Officer. The cabinet shall be referred herein as Class II, Type B2-Hard Connection and is designed to be left in the running position in a constant volume building exhaust system to maintain the space air pressure relation and to minimize the amount of exhaust CFMs discharged and save energy. If the building automation can turn off/down the exhaust airflow to the cabinet when the cabinet is not in use as determined by the cabinet operator and control interface or when the sash is closed (Energy Saving Mode) and still maintain the required space pressure, the requirement to leave the cabinet turned on 24/7 is waived. The cabinet manufacturer shall be contacted by the controls contractor to ensure that the necessary interlocks are available, compatible, and installed on the cabinet control microprocessor.

1.2 Objective The objective of this document is to provide University specifications for the procurement of Class II, Type B2-Hard Connection safety cabinets designed with a contaminated negative pressure plenum, which is contiguous to the ambient environment via a sealed metal barrier and saves exhaust energy by minimizing conditioned air requirements. The contaminated metal positive pressure plenum shall be interior to and surrounded by a negative metal pressure plenum to insure that any leakage from the positive plenum can be drawn back into the cabinet without contamination of the exterior atmosphere surrounding the cabinet. Such a cabinet will be referred to as a Class II, Type B2-Hard Connection. In addition to conforming to these minimum specifications, all cabinets offered for purchase will conform to NSF Standard 49 and display a current valid NSF-49 seal of certification unless special fabrication requirements by the University prevent compliance with this standard. In which case, the manufacturer may be requested to provide equivalent or more extensive NSF-49 Standard testing at the factory.

1.2.1 Noncompliance Cabinets which have been selected from the competitive bidding process and delivered to the

University shall conform to the specifications and standards cited in this document. In the event that the delivered cabinet does not conform to these requirements, the manufacturer shall be contacted by the University Agent to arrange for rectification of any identified non-compliance problem either in performance and/or construction specification. If the non-compliance problem cannot be resolved in 10 working days following receipt of notice, it will be the responsibility of the supplier to bear expense to re-crate and remove the equipment from the University property within the following 10 working day period. Unless prior arrangements are made, at the end of the second 10-day period the University, at its option, may have a moving and storage agency remove the equipment from the campus and store the cabinet at the manufacturer's expense.

1.3 Classification The Class II, Type B2-Hard Connection safety cabinet(s) covered by the specification shall be the following sizes:

1.3.1 Sizes

1.3.1.1 Four foot cabinet

1.3.1.2 Six foot cabinet

1.3.2 Style The Class II, Type B2-Hard Connection safety cabinet(s) covered by the specification may be specified in either a bench model or bench model with channel stand.

1.3.2.1 Bench unit is a free standing unit which can rest on a properly supported counter top or channel stand assembly. The blower motor assembly is above the work zone.

1.3.2.1.1 The channel stand supporting the cabinet static load to the floor shall be designed with at least a 1.5 safety factor with respect to the overbearing design load. The cabinet and channel stand shall be tip resistant as defined by NSF 49 Standard.



1.3.2.1.2 A bench unit may be located on a laboratory bench provided that the planned location has been reviewed and approved by a registered engineer. The supplier of the cabinet shall have optional bench top under support rails to protect and distribute the over bearing load to the receiving counter top.

1.4 Innovative Design It is the intent of this specification to limit the purchase of Class II, Type B2-Hard Connection cabinets to units with a demonstrated record of safety, performance and maintainability at the time of this writing. The University recognizes that these specifications may limit new technology development and is prepared to review all designs, which would be outside the boundaries of the specifications as currently written. If a selected supplier chooses to revise a biological safety cabinet during the duration of the contract and the University is convinced that an innovative design is acceptable via impartial testing results, supplier testing results or engineering review, a written variance to these specifications may be granted and the specifications revised. Therefore, a selected supplier contemplating changes in the safety cabinets which will conflict with the specifications as written is invited to submit documentation to the University for Paragraph 1.4, “Innovative Design” consideration. The changes in cabinet design will be reviewed and if an approval is issued, fabrication for those cabinets may be commenced. Until approval is granted, the specifications as written remain enforceable.

1.4.1 Any conflict with the specifications contained herein during the competitive bidding process, using innovative design(s) shall be identified by the supplier per each subsection of the specification with an explanation as to why the innovative design(s) is justified in terms of better safety, performance, testing and maintainability. The University may review said changes but expeditious approval within the bidding process period cannot be guaranteed. Each bidder is encouraged to adhere to the specifications contained herein to be considered for competitive selection.

1.4.2 The cabinet design shall include ergonomic considerations in the use, maintenance and certification of biological safety cabinets. Those manufacturers that display the greatest level of human engineering mechanics, conformance with this specification and conservative economic product shall be considered in the bidding process.

1.5 Definitions

1.5.1 NSF, National Sanitation Foundation

1.5.2 RPM, revolutions per minute

1.5.3 VAC, voltage - alternating current

1.5.4 CFM, cubic feet per minute

1.5.5 WC, water column

1.5.6 QT, total fan airflow

1.5.7 R, filter resistance. Filter resistance is the pressure drop in inches water column across a HEPA filter

1.5.8 ID, inside diameter

1.5.9 OD, outside diameter

1.5.10 Fail Safe Mechanism, a mechanical device that requires operator assistance to place the mechanism into service or to disconnect the mechanism from service

1.5.11 PAO-4; the default aerosol challenge and test agent of choice

1.5.11.1 DOP, dioctylphthalate, acceptable alternative aerosol challenge agent, agent of second choice

1.5.11.2 Mineral Oil, acceptable alternative aerosol challenge agent, agent of third choice

1.5.12 AMCA, Air Moving and Conditioning Association

1.5.13 LFPM, linear feet per minute

1.5.13.1 SLFPM, Standard linear feet per minute, corrected to 29.92” Hg, 70 degrees F

1.5.13.2 ALFPM, Actual linear feet per minute, corrected to actual density and temperature



1.5.14 PEP, Positive Exterior Plenum shall be defined as any plenum, which has been designed to be maintained at a pressure greater than a surrounding exterior atmosphere in order to insure the satisfactory operation of the biological safety cabinet

1.5.15 NEP, Negative Exterior Plenum shall be defined as any plenum, which has been designed to be maintained at a pressure less than a surrounding exterior atmosphere in order to insure the satisfactory operation of the biological safety cabinet

1.5.16 NPT, National pipe taper

1.5.17 FILTER VELOCITY, the air velocity measured in the media section of the HEPA filter, in a plane projection parallel with and four inches perpendicular from the media. The perimeter of the plane projection shall be offset at least three inches from any filter media obstructions

1.5.18 AVERAGE DOWN FLOW VELOCITY, the air velocity associated with cabinets designed with a validated design velocity distribution in the work zone. For cabinets designed with multiple velocity distributions in the work zone average down flow velocity is the row average with work zone hangers and UV Lamp removed

1.5.19 HEPA, high efficiency particulate air filter, a throw-away extended-media dry-type filter in a rigid frame having a maximum clean filter pressure drop of 1.0 inch water gauge at a rated airflow capacity. Each filter shall bear a filter label with the designated filter efficiency, airflow and static pressure drop. The label shall include the factory name and address, filter model and filter serial number. Approved Alternate: This specification recognizes 3.0-inch deep HEPA filters for work zone application in the BSC

1.5.19.1 ULPA, ultra-low penetration air filter, a throw away, extended medium, dry type filter in a rigid frame, having a minimum particle collection efficiency of 99.999% (i.e. a maximum particle penetration of 0.001%) for particles in the range of 0.1 to 0.2 micron when tested in accordance with the methods as outlined in IEST-RP-CC007

1.5.20 SUPPLIER, the supplier of the biological safety cabinet. Supplier is the biological safety cabinet contractor. Job site building contractors must provide only UW specification grade biological safety cabinets, alternates will be rejected. Please contact the University Agent for assistance at 608-262-1809.

1.5.21 Metal Double Wall Construction: The rear wall and side walls of the work zone are double metal with negative air plenums in between. The inner wall is contiguous with the work zone and the outer most wall is on the exterior side of the cabinet

1.5.22 Closure Panel, the barrier between a positive or negative pressure plenum in the cabinet and the ambient environment or negative pressure zone inside the cabinet. Exterior closure panels are referred to as reinforced panels which have been provided with turned edges to increase stiffness and reduce fastener spacing. Interior closure panels are flat panels with very little additional stiffness and generally have welded studs and fasteners in a highly repetitive sequence. Dress panels are cosmetic to cover maintenance areas of the cabinet. These panels are held in position with metal tabs using gravity assist or limited number of fasteners.

1.5.23 HP, horsepower

1.5.24 LASKIN NOZZLE, see National Sanitation Foundation Standard No. 49

1.5.25 Performance Envelope, the range of airflow velocities (intake versus down flow) within which a biological safety cabinet will pass the latest edition in effect of NSF-49-2002e microbiological aerosol tests for product protection and personnel protection.

1.5.26 Barometric Pressure, BP, Local air pressure as inches mercury

1.5.27 Port, a resealable mechanical penetration into the cabinet interior to provide a service entrance for aerosol testing, pressure measurement, damper control or decontamination agent

1.5.28 University Agent, One designated individual with an engineering degree, formal training in the certification of biological safety cabinets, recommended Active Professional Engineering Registration, and recent listing as an Accredited NSF Field Certifier.

1.5.29 Hard Connection, An exhaust transition that is designed to be sealed to the cabinet and to the building exhaust system

1.5.30 SF, square foot



1.5.31 Gel Seal, A flexible seal between the filter frame and the filter mating surface. Gel seal, also known commercially as “Blue Gel”, is non-flowing, non-hardening, and is applied in either direction vertically. The gel is silicone base and is applied by the filter manufacturer. Gel seal has been evaluated at UW and has demonstrated that the physical properties do not change during the service life of the filter. Silicone base gel is the only acceptable gel for biological safety cabinet application. Polyurethane gel is prohibited.

2.0 APPLICABLE DOCUMENTS

2.1 Specifications and Standards The following documents, of the issues in effect on the date of the invitation for bids or requests for proposal, form a part of this specification to the extent specified herein:

International Standards

ISO 14644 -1 Clean rooms and Associated Controlled Environments

National Standards

National Sanitation Standard Number 49, the latest edition in effect

National Sanitation Standard-Biosafety Cabinet LISTING, the latest edition in effect

Federal Specifications

J-C-145 Cable, Power, Electrical and Wire, Electrical; (Weather Resistant)

W-C-00596 Connector, Plug, Electrical; Connector Receptacle, Electrical

W-S-00896 Switch, Toggle

W-S-893 Switch, Toggle, and Mounting Strap, (Interchangeable)

CC-M-636 Motor, Alternating current, (Fractional Horsepower)

DD-G-1403 Glass, Plate (Float), Sheet, Figured, and Spandrel (Heat Strengthened and Fully Tempered)

QQ-S-698 Steel, Sheet and Strip, Low carbon

QQ-S-766 Steel Plates, Sheets, and Strip-corrosion Resisting

TT-C-490 Cleaning Methods and Pretreatment of Ferrous Surfaces for Organic Coatings

TT-C-535 Coating, Epoxy, Two Component for Interior and Exterior Use of Metal, Concrete and Masonry

TT-C-001224 Coating System, Epoxy, Glaze for Interior Surfaces

TT-C-001227 Coating System, Polyurethane Glaze for Interior Surfaces

PPP-B-601 Boxes, Wood, Cleated plywood

PPP-B-621 Boxes, Wood, Nailed and Lock corner

PPP-B-640 Boxes, Fiberboard, Corrugated, Triple wall

PPP-C-650 Crates, Wood, Open and covered

PPP-C-843 Cushioning Material, Cellulose

PPP-T-60 Tape, Packaging, Waterproof

Federal Standards

Federal Standard No. 102, Preservation, Packaging and Packing Levels

Federal Standard No. 123, Marking for Domestic Shipment

Military Specifications

MIL-C-104 Crates, Wood; Lumber and Plywood Sheathed Nailed and Bolted



MIL-C-132 Crates, Wood, Open; Maximum Capacity 2,500 pounds

MIL-C-3774 Crates, Wood, Open; 12,000 and 16,000 Pound Capacity

MIL-F-51079B Filters, Particulate, High Efficiency, Fire Resistant, Biological Use

MIL-L-10547 Liners, Case and Sheet Overwrap, Water-vaporproof or Waterproof, Flexible

MIL-P-116 Preservation, Methods of

MIL-R-3065 Rubber, Fabricated Products - Gaskets, Synthetic Rubber

MIL-S-8802 Sealing Compound, Temperature resistant, Aircraft High Adhesion

Military Standards

MIL-STD-282 Filter Units, protective clothing, gas mask components and related products

Performance Test Methods

ISO 14644-1 AND 2 – Cleanrooms and Associated Controlled Environments

2.2 Other Publications

Air Moving and Conditioning Association (AMCA)

AMCA 99-76 Standards Handbook

AMCA 210-74 Test Code for Air Moving Devices

AMCA AS-2406-66 Fans, Designation of Direction of Rotation and Discharge

AMCA 211-80 Fans, Labeling Requirements

AMCA 401-66 Classifications for Spark Resistant Construction

American National Standards Institute, Inc. (ANSI)

A11.1-120083 Industrial Lighting RP-7

N101.1-120082 Efficiency Testing of Air Cleaning Systems Containing Devices for Removal of Particles

S1.2-1962 (R120081) Method for the Physical Measurement of Sound

S1.4-120081 Specification for Sound Level Meters

S2.2-1959 (R2011) Methods for the Calibration of Shock and Vibration Pick-ups

Z97.1-2009 Performance Specifications and Methods of Test for Safety Glazing Material Used in Buildings

American Society Mechanical Engineers (ASME)

AG-1 Code on Nuclear Air and Gas Treatment

Illuminating Engineering Society (IES)

IES Lighting Handbook

National Electrical Code (NEC)

Electrical Component and Wiring Specification

National Electrical Manufacturer's Association (NEMA)

Underwriters Laboratories (UL)

UL-817 Cord Sets and Power Supply Cords

UL-62 Flexible Cord and Fixture Wire



UL-586 High Efficiency Particulate Air Filter Unit

UL-900 Safety Standards for Air filter Units

UL-2701-1 Electrical Safety and Integrity

United States Atomic Energy Commission (AEC)

ORNL-NSIC-65 (1/70) Design, Construction, and Testing of High Efficiency Air Filtration Systems for Nuclear Applications

Canadian Standards Association (CSA)

C22.2 No. 151-1979 Laboratory Equipment

CAN/CSA-Z316.3-M87 Biological Containment Cabinets: Installation and Field Testing

Institute of Environmental Sciences and Technology (IEST)

IEST-RP-CC-001.5 HEPA and ULPA Filters

IEST-RP-CC-007.2 Testing ULPA Filters

IEST-RP-CC-013.3 Calibration Procedures and Guidelines for Selecting Equipment Used in Testing Cleanrooms and Other Controlled Environments

3.0 REQUIREMENTS

3.1 Bid Submittal Requirements The following requirements apply to cabinets bid on an individual basis. For cabinets procured via a purchasing contract, the selected manufacturer need only comply with Sections 3.1.1, 3.1.2 and 3.1.3 for the initial bid; unless the cabinet fan/motor system is changed during the contract period. In this event, revised motor and fan graphs shall be resubmitted and approved by the University Agent prior to construction of any modified cabinet as represented in the original contract. Sections 3.1.4, 3.1.5 and 3.1.6 shall apply to all cabinets received by the University.

3.1.1 Motor and Fan Graphs Detailed motor and fan graphs for each safety cabinet shall be submitted with each bid to the Purchasing Department. The graphs shall include sufficient detail to permit the University to determine if the selected fan/motor combination will comply with requirements in Section 3 of this specification. Sample graphs detailing the recommended format are included in Appendix A, Graphs 1 and 2. ACCEPTABLE ALTERNATIVE: As an alternative to the graphs included in Appendix A, graphs and data tables can be provided in an alternate format given that the graphs/data tables detail the following:

1. Motor manufacturer2. Motor model, horse power, and capacitor size3. BSC model in which the motor is to be installed, including appropriate details such as sash height,

presence or lack of prefilters, etc.4. Operational airflow data in CFMs associated with required air velocities detailed in Paragraph 3.75. Initial fan static pressure using clean filters and associated motor RPMs and amperage6. Fan static pressure, associated motor RPMs, motor amperage, representative airflows in CFMs, and

change in total airflow detailing 50%, 100%, and 200% filter loadingThis requirement shall be fulfilled by actual testing of the biological safety cabinet offered for purchase or testing of a prototype model of identical design and filters.

3.1.1.1 Motor Performance Graph(s) Submitted bid(s) shall contain a motor performance graph selected by the supplier for the equipment to be used in the cabinet(s) proposed under bid. Each graph shall list the motor manufacturer, model number, horsepower and capacitor size. The ordinate of each graph shall contain units for motor RPM. The abscissa of each graph shall contain units for torque, voltage and amperes with applicable multipliers. At least three curves shall appear on the graph:

1. Motor torque versus RPM.2. Motor voltage versus RPM.3. Motor amperes versus RPM.

3.1.1.2 Fan/Motor Performance Graph(s) Submitted bid(s) shall contain fan/motor performance graph(s) for the equipment selected by the supplier to be used in the cabinet(s) proposed under bid. Each graph shall detail the fan manufacturer, fan model number, motor manufacturer and motor model number. The ordinate of each graph shall contain units for static pressure in inches water gauge. The abscissa of each graph shall contain units for cubic feet per minute of air at standard conditions (70 degrees Fahrenheit, sea level datum). The following elements shall appear on each graph:



3.1.1.2.1 A series of constant fan wheel speed curves in RPMs.

3.1.1.2.2 A series of constant fan wheel torque curves in ounce feet.

3.1.1.2.3 A discrete fan/motor torque curve with the motor speed control(s) at full line voltage - 115 VAC. The curve points shall be obtained from the motor performance graph.

3.1.1.2.4 The fan design curve shall be such that the loaded and unloaded system resistance curves intersect the fan characteristic curve in the negative sloped portion of the fan curve. A Fan/motor designed to operate at the specified airflows on either the broad or flat portion of the fan curve - i.e., positive slope - may be rejected depending on how the performance characteristics of the exhaust and supply air velocities are effected when the filters have been loaded in accordance with specification 3.1.1.2.5.5.

3.1.1.2.5 A discrete fan/motor torque curve with the motor speed control adjusted to the operational airflow in CFMs associated with required air velocities detailed under Paragraph 3.7. The curve points for reduced voltage operation may be approximated from the following formula: torque 1/torque 2 = (voltage 1/voltage 2)2 or with actual motor performance data. The manufacturer shall indicate on the fan/motor performance graph how the curve points for reduced voltage operation have been derived and submit motor performance data for reduced voltage operation if used as curve points.

3.1.1.2.5.1 The curve generated under Paragraph 3.1.1.2.5 shall detail a representative initial fan static pressure using clean filters and a representative operational motor voltage.

3.1.1.2.5.2 The curve generated under Paragraph 3.1.1.2.5 shall detail the fan static pressure associated with a 100 percent increase in filter static pressure. A 100 percent increase in filter static pressure shall be determined by multiplying the clean filter static pressure, exhaust or supply filter static pressure, whichever is greater, by 2.0. The static pressure across each filter is that pressure necessary to develop the design mass filter airflow rate per filter so that the air velocity requirements specified in Paragraph 3.7 are achieved.

3.1.1.2.5.3 The curve generated under Paragraph 3.1.1.2.5 shall detail the fan airflow rate associated with a 10 percent drop in operational total airflow rate in CFMs.

3.1.1.2.5.4 The manufacturer shall detail on the fan/motor performance graph a representative initial filter pressure drop at the total fan airflow associated with average down flow and face velocities required under Paragraph 3.7. This requirement shall be fulfilled by actual testing of the biological safety cabinet offered for purchase or testing of a prototype biological safety cabinet with clean HEPA filters with resistance and flow characteristics which are within ± 5 percent of those HEPA filters offered in the production biological safety cabinet. The total airflow in CFM, the motor speed in RPM and filter static pressure in inches W.C. at this set point shall be stated. This detail shall appear on the fan/motor performance graph using the following format:

Clean Filters with Cabinet Air Velocities at Factory Set PointQT CFM, Motor Speed RPMs, Filter R "W.C.

3.1.1.2.5.5 With the supply and exhaust filters evenly loaded to produce a 10 percent reduction in the clean filter total airflow delivery detailed in Paragraph 3.1.1.2.5.4 the manufacturer shall detail the resultant airflow in CFM, motor speed in RPMs and filter static pressure in inches W.C. This requirement shall be fulfilled by actual testing of the biological safety cabinet offered for purchase or testing of a prototype model of identical design and filters. The test results shall appear on the fan/motor performance graph using the following format:

Loaded Filters with 10percent Reduction in Total Airflow QT = CFM, Motor Speed RPMs, Filter R "W.C.

3.1.2 Fabrication Drawings and Performance Envelope

3.1.2.1 Fabrication Drawings One set of detailed fabrication drawings shall be submitted with each bid to the University Purchasing Department. Drawings shall include sufficient detail to permit the University of Wisconsin to determine if all physical construction, mechanical, electrical and dimensional requirements in Section 3 have been included in the fabrication plans. Sample figures with dimensional requirements are included in Appendix B, Figure 1.

3.1.2.2 Performance Envelope Graph The submitted bid shall contain one performance envelope graph detailing the cabinet's ability to effectively handle biological aerosols under multiple velocity conditions.



The graph data shall provide a statistical performance envelope graph plotting intake air velocity in feet per minute on the ordinate of the graph and supply air velocity in feet per minute on the abscissa.

3.1.2.2.1 At least 20 data points shall be displayed on this graph.

3.1.2.2.2 The vendor's model number shall be displayed on this graph.

3.1.2.2.3 The graph shall show passing results of the NSF-49 Personnel Protection test at set point and at cabinet airflow velocities -15 fpm (±3 fpm) inflow and +15 fpm (±3 fpm) down flow from set point. The graph shall also show points where the cabinet fails.

3.1.2.2.4 The graph shall show passing results of the NSF-49 Product Protection test at set point and at cabinet airflow velocities +15 fpm (±3 fpm) inflow and -15 fpm (±3 fpm) down flow from set point. The graph shall also show points where the cabinet fails

3.1.2.2.5 The graph shall detail the cabinet set point velocity, defined as the midpoint velocity for intake and the midpoint velocity for down flow. The NSF-49 velocity ranges for down flow and intake shall also be labeled on the graph. For zone supply flow biological safety cabinets submit average row velocities.

3.1.2.2.6 A statistical average and upper and lower ninety-five percent confidence limits shall be displayed on the graph.

3.1.2.2.7 The data shall be reviewed by the University to insure that the biological safety cabinet under bid can safely perform under an array of air velocity conditions.

3.1.2.2.8 Failure to submit performance envelope data as called for in Paragraph 3.1.2 shall result in bid rejection.

3.1.3 Submitted Bids Submitted bids will be reviewed within 60 days of receipt and the successful bidder notified accordingly by the University.

3.1.3.1 Any bid, which does not comply with the specifications and performance standards contained herein, may be rejected.

3.1.3.2 Any bid which does not format requested data within the requirements of Paragraph 3.1 shall be rejected.

3.1.3.3 Any bid containing proposed fan/motor systems sized and rated such that the operational air delivery required under Paragraph 3.5.6.3 cannot be obtained shall be rejected.

3.1.4 Selected Supplier The supplier selected to construct a biological safety cabinet shall submit one set of detailed final fabrication drawings to the University. The drawings shall include sufficient detail to permit the University to determine if all physical construction, mechanical, electrical and dimensional requirements in Section 3 have been included in the fabrication plans. Electronic drawing submittal shall be the preferred method of submission in order to comply with this requirement.

3.1.4.1 Any optional equipment specified by the University for bidding shall be detailed on the final fabrication drawings.

3.1.4.2 The selected vendor shall commence cabinet fabrication after receiving written and/or verbal approval to proceed from UW Environment, Health, and Safety – Engineering Technical Services.

3.1.4.3 Once approval for final fabrication drawings has been given, the University shall permit no variance in the construction without prior approval. Contact the University Agent for questions regarding variance.

3.1.5 Field Fan/Motor Performance Test The cabinet(s) selected for purchase may be subjected to a field fan/motor performance test during performance testing of the cabinet. If this test demonstrates a failure of the fan/motor system to comply with the operational air delivery required under Paragraph 3.5.6.3, the cabinet manufacturer shall be contacted to arrange for replacement of the cabinet or purchase cancellation. Those cabinets returned to the supplier shall be at the supplier's expense (FOB BSC field location). Arrangements and costs for packaging of the cabinet for shipment will be the responsibility of the supplier.

3.1.6 Field Internal Plenum Pressure Test The cabinet(s) selected for purchase may be subjected to a field internal positive pressure plenum test in addition to other performance testing of the cabinet. If this test demonstrates a failure of the plenum to comply with the allowable leakage rate required under Paragraph 3.5.6.6.1, the cabinet manufacturer shall be contacted in order to arrange for repair, replacement or cancellation.



3.1.7 Field Specification Compliance Inspection The cabinet(s) selected for purchase may be subjected to a field compliance inspection to determine whether the manufacturer has complied with the construction specifications contained herein. The cabinet may be completely dismantled and any conflicts with the construction brought to the attention of the supplier, as detailed in Paragraph 1.2.1.

3.2 General Description

3.2.1 Complete Unit: The cabinet shall be a complete unit, free standing, fabricated with materials of construction as required and contain all items of equipment as necessary to be functional and to operate fully within the intent of this document.

3.2.1.1 The cabinet may be either a bench with supporting channel stand (floor standing unit) or bench top (unit which may be mounted on a laboratory bench after approval has been issued). Specific requirements for the particular cabinet under bid will indicate whether a bench with support frame or bench model is required. Bench top location shall be pre-approved by a registered engineer.

3.2.1.2 Metal double wall construction: The cabinet liner shall be surrounded by metal double wall construction on the rear and left and right work zone sidewalls. The inner section of the metal double wall shall serve as a negative air plenum and service area for plumbing and electrical services. Single wall construction is prohibited in these areas.

3.2.1.3 One Cable Port Required: The right sidewall shall be provided with a sealable and cleanable port to allow external wiring and tubing to pass into and out of the safety cabinet work zone without having to enter under the sliding sash. A second cable port on the left sidewall may be specified as an option.

3.2.2 Independent Unit: The cabinet, when connected to external source of electrical power, building exhaust system, and building automation control, if necessary, shall be completely independent of all other sources for successful operation and performance. The owner shall provide an exhaust ventilation system design for 675 CFM for the four foot cabinet and 1000 CFM for the six foot cabinet. The exhaust ventilation shall be capable of providing at least 1.9” water column for the four foot cabinet and at least 2.3” water column for the six foot cabinet with loaded filter conditions.

3.2.3 Window Edge Protection: The working face of the unit shall be a panel of safety plate glass or tempered glass with exposed edges contained in a polished stainless channel. The lower edge of the window shall be enclosed in a 24-gauge stainless steel channel, satin finish number 3. The use of window bumpers are not considered an alternative to having the upper window channel. The supplier shall provide two upper frame bumpers and upper 24 gauge stainless window strip and Teflon gliders to prevent window breakage upon lifting to the end of travel. Lower window stops/bumpers and rail channels shall also be provided to prevent window breakage upon lowering. If the cabinet is supplied with a UV lamp assembly, the interlock switch shall open the switch when the viewscreen is not fully closed.

3.2.4 Directional Air: The cabinet shall consist of a partially enclosed workspace into which air, which has been filtered by HEPA filter(s), is supplied in a near unidirectional vertical manner downward through the workspace, with an air curtain located behind the sash. Laboratory air will be drawn into the cabinet work access opening and be removed by a front horizontal perforated intake grill integral to the work tray. No intake air will pass directly onto the cabinet stainless steel work tray. Scavenger exhaust slots shall be provided on the left and right vertical airfoils that form the entrance to the work access opening and along the upper section of the sash.

3.2.5 Work Area: The work area shall consist of the following items going from front to back: the work access opening (Paragraph 3.4.4), the horizontal airfoil/arm rest, left and right vertical airfoils with scavenger slots, the perforated stainless steel sheet area (Paragraph 3.2.6) which is integral to the removable 16-gauge solid stainless steel work tray, the perforated stainless steel sheet air exhaust area located in the rear wall or rear work area. The free open area of the front and back perforated areas shall divide the supply airflow (Paragraph 3.7.4) and permit the supply air to pass through, with a minimum amount of turbulence, into the negative pressure plenum which is connected to the suction side of the building exhaust fan. The top lip of the armrest shall be at least one inch above the removable solid stainless steel work tray. The removable work tray shall be the full length of the workspace from wall to wall (Paragraph 3.3.2). It shall extend from the front armrest to the rear wall. It shall be flat and level. The solid stainless steel removable work tray shall have a perimeter lip of 0.625-inch (+0", -0.125"), and the remainder of the solid stainless steel work tray shall be depressed 0.75 inch (+0.125", -0"). The flatness shall be parallel to the plane of the base of the cabinet ± 0.0625 inch at all points. The discharge side of the supply filter shall be provided with a stainless steel diffuser, (Paragraphs 3.5.2.5 and 3.7.3).

3.2.6 Work zone Perforated Exhaust Grills: Front and rear work zone air exhaust grills: A front perforated stainless steel sheet area shall be located in the area between the removable horizontal airfoil/arm rest and the solid work tray in the work area. The front exhaust air grill shall be integrated into the removable worksurface. Air shall leave the workspace via the front perforated area and via a rear perforated opening extending the full length of the workspace in the rear wall just above the work tray or rear section of the



horizontal work area. The air removed via the front and rear perforated areas shall comply with Paragraph 3.7.4.

3.2.7 Scavenger Slots: The left and right sides of the work zone intake shall be provided with at least 10 - ½” x 3/16” punched slots per side. The slots shall be located directly in the corners of the airfoil and extend at least half an inch on both sides. Scavenger slots shall be connected to exhaust plenum by sheet metal construction. Approved Alternative: Scavenger slots may be located on the interior walls of the work zone, but no more than 0.5 inch in from the vertical entry plane.

3.2.8 Drain Spillage Trough: A stainless steel perimeter drain spillage trough shall be located below the work surface arranged as to catch any spillage dropping below the working area. The outer perimeter dimensions of the trough shall be at least the same perimeter dimensions of the work tray and front perforated area combined. With the solid work tray/front perforated area removed plus removable support cross members and rear wall perforated sheet, all areas of the trough shall be accessible for cleaning. The trough corner construction shall have at least 0.125-inch radius corners. The supporting structure shall be at least 2.50 inches above the floor of the trough. The drain spillage trough shall accommodate a minimum of five liters and be liquid tight.

3.2.8.1 A stainless steel, metal or wire guard shall be provided over the open horizontal airflow area that forms the rear and sidewall wall inlet plenum and is located within six inches of the work zone. The guard shall have uniform openings no greater than one square inch. Approved Alternative: The guard can have openings no wider than 0.5 inches and no longer than 8 inches.

3.2.8.2 The edges of the guard shall be tear and puncture resistant to laboratory gloves. Field experience has demonstrated that this area of the cabinet requires periodic cleaning and will catch paper that must be removed by gloved hands.

3.2.9 Exhaust Filter Protector: Exhaust filter shall contain a fixed protective screen on the downstream side.

3.2.9.1 Cabinet exhaust damper: Each cabinet shall be provided with a high integrity (air tight) shutoff damper in order to completely isolate the cabinet from the building exhaust ventilation system. The damper shall be stainless steel, cleanable, and have no sharp edges that could harm certifiers or maintenance personnel. The damper shall pass the glove tear test, Paragraph 3.5.2.4.2. The damper shall be gasketed and provided with each cabinet. The damper diameter shall be 12 inches ±0.125 and provided with a flex sleeve and stainless hose rings to connect it to the house exhaust system.

3.2.9.2 The interior side of the exhaust HEPA filter frame shall be contiguous to the cabinet negative pressure plenum.

3.2.9.3 Exhaust filter removal shall be designed to be removed from the front of the biological safety cabinet access opening.

3.2.9.4 The exhaust filter face must be completely accessible. Clamp assemblies which protrude across the filter face shall not be allowed.

3.2.10 Reinforced Panels: Reinforced/closure panels shall be provided to allow front access to the supply and exhaust filter, as applicable and front access to the fan/motor. The fan plenum closure panel shall have a gasket as detailed in Paragraph 3.5.5.4 and shall conform to leak tight construction in Paragraph 3.11.

3.2.10.1 Top, front, or side closure panels shall be provided for access to sash balance mechanism, utility plumbing lines, and electrical components.

3.2.10.2 All closure panels to the filters and fan/motor shall be designed so that each panel can be removed from the cabinet without obstruction from adjacent closure panels and associated fasteners. Dress panels are exempt from this requirement if less than 6 fasteners are used to secure the panel. Dress panels shall allow access into non-contaminated areas of the cabinet structure to perform routine mechanical, electrical, or plumbing service, which is ancillary to the cabinet fan, filters, and air passages.

3.2.10.2.1 When replacing the supply filter through a front access panel, a minimum horizontal clearance of 0.25 inches and vertical clearance of 2 inches between any frame member of the cabinet and the outside vertical and horizontal dimensions of the filter frame shall be provided.

3.2.10.2.2 When replacing an exhaust filter with a removable work zone tray, a minimum lateral clearance of 0.25 inches front to back and side to side with any cabinet member shall be maintained to facilitate certifier safety in removing filters.

3.2.10.3 All front mounted closure panels using fastener screws and seal rings shall be designed to be mounted into final attachment position and stay in place independently, before fasteners are applied, to reduce



the risk of fall accidents. Approved Alternate: If a reinforced front closure panel is provided, a ledge to rest upon during installation is acceptable. Closure panels that do not fit uniformly onto the mounting fasteners are a fall hazard. The supplier shall provide sheet metal fasteners on all access panels to ensure structural rigidity against bending failure on removal and to maintain uniform gasket pressure on installation.

3.2.10.3.1 Fasteners for attaching reinforced service panels shall be ¼-20 hex head zinc plated steel screw and flat seal washer. The seal washer shall have a rubber interior seal designed to pass the NSF-49 cabinet leak test. Standard flat panel construction, if used in air passages, shall be welded stud construction using stainless studs and fasteners at 3.5 inch centers.

3.2.10.3.2 Reinforced service panel screws shall not contact any filter surfaces.

3.2.10.3.3 Each reinforced service panel shall be provided with a neoprene gasket between the panel and cabinet frame.

3.2.10.4 The cabinet exhaust filter assembly shall be surrounded by or directly connected to a negative pressure plenum. The negative plenum shall be designed to leak tight construction as defined in Paragraph 3.11.

3.2.10.5 Supply Filter Access Panel: The supply filter access panel shall be front access and provided with at least a ½”- 1” turned edge, welded at the corners. The panel shall be secured in place with evenly spaced metal fasteners using a minimum fastener size ¼ x 20 using plated steel bolts and sealing washer.

3.2.10.5.1 The supply filter access panel gasket shall be at least ½ inch x ¼ inch neoprene permanently bonded to the inlet flange of the supply filter plenum or reinforced access panel flanges.

3.2.10.6 Exhaust Filter Access Panel: If an access panel is provided, the interior panel shall be held in position with stainless steel welded studs at maximum seven inch spacing. The bolting unit will be stainless steel 11/32-inch nut, lock washer and flat washer. The panel shall compress against a ¾- inch gasket attached to the plenum entrance flange.

3.2.10.6.1 Approved Alternative: If the exhaust filter frame is surrounded by a negative air plenum, the upstream and downstream face shall be provided with a filter gasket to connect the filter to the blower plenum. The filter manufacturer shall install the gasket.

3.2.11 Construction and Fabrication Requirements

3.2.11.1 Strength and Rigidity Requirement: The cabinet shall be constructed with the strength and rigidity to meet noise and vibration level, and glove tear resistance specified in Paragraphs 3.9, 3.10, and 3.5.2.4.2. The liner shall be one-piece stainless steel construction for the rear and sidewalls using 16 gauge stainless steel, with metal double wall construction, integral radius rear corners with non-glare stain No. 4 metal finish to diminish reflection, and integral exhaust slots above the sash and at the operator entrance to the work zone. A 16 gauge stainless steel work surface with rounded corners for cleaning and attached integral front intake grill shall be provided along with a recessed work trough for liquid spills. The internal metal ducts shall be 16 gauge steel, external reinforced panels shall be 16 gauge steel. Dress panels shall be 18 gauge steel. Multiple panel construction attached with fasteners and sealant bonding for the work zone is prohibited. Option: The supplier shall offer an all stainless steel construction if called for in the purchase request.

3.2.11.1.1 Bypass armrest with pad is required.

3.2.11.1.2 Footrest is optional. The footrest shall be adjustable.

3.2.11.2 Ease of Cleaning, Fasteners and seal, Gliders, Support Frame, and Adapter Rails: The cabinet shall be constructed in a manner making it easy to clean. The cabinet shall be provided with removable filter diffusers, lift out work trays and support struts, and cabinet base spillage drain. Cracks or crevices, which provide reservoirs for access of contamination, shall not be accepted. The two rear vertical corners in the work zone shall radii corners with a minimum radius of 0.4375 inch. The liner in the work zone shall be one-piece stainless steel construction with joints continuously welded and buffed.

3.2.11.2.1 All interior nuts, bolts, machine screws, stud screws, stud bolts, etc., shall be stainless steel. Exterior screws used to seal the reinforced panels shall be stainless steel with zinc plated screws as the alternate. All fasteners associated with the stainless steel liner shall be stainless steel and smooth. Fasteners in the work zone shall be glove tear resistant. This shall include fasteners for the window sash assembly. If welded stud bolt construction is used, spacing should be no more than 3.5 inches center to center. The removable rigid panels used in the



contaminated zones shall be designed with dual integral ½ -2-inch ninety degree turned lips which have been formed and welded at the corners and the metal de-burred, polished, painted and gasket. Rigid panel construction will have reduced fastener spacing to 14-inch center to facilitate access using 1/4x20 bolts, washers and seal rings. The holes in the service panels and closure plates and sections of the cabinet shall be sized to prevent panel hole from contacting the screw fasteners during assembly of the panel or assembly. The method in mounting the multi-section cabinets together shall be by the use of sealable o-ring bolts or by the use of electric welded stainless steel studs. The bolting unit shall be at least 11/32-inch stainless steel hex or cap nut; 0.125-inch ID stainless steel split lock washer. Where the stud protrudes through to the outside of the cabinet the same construction applies with the exception that only stainless cap nuts can be used to protect against the exposed studs. Approved Alternative: If the upper and lower sections of the cabinet consisting of the filter plenums and blower are permanently joined by continuous piece of fabricated metal liner or by continuous welded seams, electric welded stud requirement is waived.

3.2.11.2.1.1 The holes in the service panels, closure plates and separable sections of the cabinet shall be at least 10 percent larger in diameter than the stud diameters. All exterior panels and fasteners shall be fabricated to facilitate required leak tight construction and be easily removed without need of extraordinary measures because of miss-aligned holes and studs. Miss-aligned stud/hole is construction poor workmanship and will result in either a 1.2.1 notification or corrective repairs as covered in paragraph 6.3.4.

3.2.11.2.1.1.1 Cable Port The right work zone side shall be provided with a cable port to allow for tubes and power cords installed by the user so that the sash can be completely closed. Option: A second cable port on the left side wall is optional.

3.2.11.2.1.3 Phillips head screws and plastic molded fasters shall not be allowed. Use of said construction will result in a 1.2.1 notification.

3.2.11.2.1.4 Cabinets provided with non-metal interior positive pressure plenums are prohibited and will result in a 1.2.1 notification.

3.2.11.2.2 All machine screws and clamping mechanisms used to secure access panels or internal plenums shall be designed to apply torque to compress the panel gaskets in accordance with Paragraph 3.5.5.3.1. In the event that more than one machine screw fails per closure plate either in the slot head, shaft or threads in affecting the necessary compression torque, the manufacturer will be notified in accordance with Paragraph 1.2.1.

3.2.11.2.3 Gliders: The Class II, Type B2 – Hard Connection safety cabinets with channel stands shall have adjustable gliders installed in the base of the cabinet. The gliders shall be heavy enough to support the weight of the cabinet during shipment, moving and set-up at the point of use. The gliders shall have at least 0.50-inch threaded shafts with stainless steel bottom on glider with nylon cap.

3.2.11.2.3.1 Glider construction shall be stainless steel, adjustable height and the glider foot shall be rounded to reduce floor tile damage on movement. Glider feet with sharp corners at the floor contact point that damage the floor will result in a 1.2.1 notification and the supplier required to replace the parts. The supplier must pay close attention to glider construction since many floors in biocontainment facilities are now being provided with soft floor coverings.

3.2.11.2.3.2 Channel Stand: Bench with support frame unit: The support frame shall be provided with telescoping legs to allow the cabinet to be used in a sit-down, stand-up position and midway between these positions. The elevation of the work surface shall be either 30 inches (±1.0 inch) in the lower position or 36 inches (±1.0 inch) in the standing position. Specific bid requirements may specify different work surface elevations. The supplier will provide optional holes in the supporting legs to achieve the desired work zone heights outside the 30 to 36 inch range if requested in individual purchase.

3.2.11.2.3.2.1 The supplier shall design all channel stands to a safety factor of at least 1.5 for vertical overbearing load.

3.2.11.2.3.2.2 The channel stand shall be bolted to the cabinet.

3.2.11.2.4 Optional Adapter Rails for Laboratory Bench Location: If the cabinet is to be placed on an owner supplied laboratory bench, the manufacturer may be requested to provide adapters to distribute the cabinet vertical load onto the bench. Point loads shall be avoided in order to reduce the risk of counter top failure and fall accidents.



3.2.11.2.5 No optional laboratory lift shall be permitted.

3.2.11.2.6 No optional wheels, push handles and bumpers shall be permitted.

3.2.11.3 Dimensional Tolerances

3.2.11.3.1 Linear dimensions shall be within ± 0.0625 inch of the specified dimension.

3.2.11.3.2 The square-ness of the cabinet shall have the overall outside and inside surface perpendicular within the following limits: the maximum offset from 90 degrees shall be 0.0625 inch or 0.03125 in/ft, whichever is greater.

3.2.11.3.3 The flatness of the outside surfaces, which can be mated to other equipment and/or furniture, shall be flat within 0.125 inch maximum offset in 96 inches.

3.2.12 Fabrication Welding Requirements

3.2.12.1 Prior to welding, work shall be degreased, buffed, and brushed as necessary to remove all dirt, scale corrosion, dust, grease, oil, water or other foreign material. All buffing shall be done with a stainless steel brush. In no case shall a carbon steel brush be used.

3.2.12.2 Weld surfaces as deposited shall be smooth and uniform in appearance without abrupt changes in contour.

3.2.12.3 Weld metal and base metal shall be free of splatter due to too high a welding current, too large an electrode, wrong electrode angle, etc., when using the base metal consumable electrode with inert gas shielding for the welding method.

3.2.12.4 Weld surfaces, root or face, shall not show oxidation. Oxidation, as here defined, shall mean granulation (sugary) or scaling of the metal that cannot be removed or restored to a bright metal surface by wire brushing. Heat discoloration or blackening by flux residue is not considered oxidation.

3.2.12.5 A crevice on any weld surface in any weld joining (other than between "skip" welds on lap joints) is not acceptable. (A crevice is defined as a space or depression, which will retain liquid, by capillary attraction, generally having a depth greater than its smallest surface dimension.)

3.2.12.6 Arc burns on the base material are not acceptable. (Arc burns are defined as scars left where the welding arc has been struck on or dragged across the base metal surface.)

3.2.12.7 Welds shall be entirely free of evidence of lack of fusion or cold lap, pinholes, cracks and weld craters, and shall be free of porosity, slag inclusion, and other imperfections as, in the opinion of the University, is obtainable by exercise of the high skills of the art.

3.2.12.8 Suck-back on the root face of welds made from one side only shall not exceed five percent of the base metal thickness. Suck-back, as here defined, shall mean a minor depression in the center of the weld-metal surface, as measured from the base metal surface, caused by metal solidification, shrinkage and/or action of gravitational forces on the molten metal.

3.2.12.9 All welds welded from one side only (except lap joints) shall have l00 percent penetration with melt-through. For butt joints, the weld metal on the front surface shall in no place be lower than the adjacent base metal surfaces; for butt, corner and tee joints, the weld metal on the back side shall in no place show a depression lower than the adjacent base metal thickness. Weld metal protrusion on the backside of welds made from one side shall not exceed 20 percent of the adjacent base metal thickness or 0.09375 inch, whichever is less. Joints detailed to be welded from one side only may, at the option of the Seller, be welded from both sides to obtain 100 percent penetration, provided that no warping in the sections being joined is caused thereby. All tolerance requirements shall be met as previously mentioned in Paragraph 3.2.11.3.

3.2.12.10 All welds shall develop the full strength of the lighter of the sections being joined, except where only a "tack" or "seal" weld is required.

3.2.12.11 All interior work area welds shall be finished flush with adjacent surfaces and shall be no rougher than adjacent surfaces. Exterior weld areas shall be ground smooth and flush with adjacent surfaces where necessary to provide gasket-seating surfaces. Finishes made for this or other reasons shall be no rougher than the finish on adjacent surfaces. All interior air plenum welds shall be finished smooth to within ±0.01 inches.



3.2.12.12 The method of choice for permanent joints in the sheet metal comprising the boundaries of the air passages will be continuous welds. Permanent joints in the sheet metal comprising the boundaries of the air passages made by tack welding and sealant bonding are optional provided that permanent joints can conform to leak tight construction, Paragraph 3.11.

3.2.12.13 Only the following two welding methods may be used for welding of stainless steel. The method chosen shall take into account all other requirements of this Specification.

3.2.12.13.1 Tungsten non-consumable electrode with inert gas shielding, using direct current and straight polarity (work positive).This method is commonly known as "TIG" or "HELIARC."

3.2.12.13.2 Base metal consumable electrode with inert gas shielding, using direct current and straight polarity (work positive). This method is commonly known as "MIG" or, when straight polarity is used, "SIGMA."

3.2.13 Services in Air Passages

3.2.13.1 Wires, tubing, cables, conduits, pipes, etc. shall not be located or routed within the air passages of the cabinet with the exception of fan electrical connections, rear wall UV lamp connections, tubing associated with aerosol ports, and any tubing necessary for airflow and/or pressure measurement.

3.2.13.2 Exception construction shall have gas tight connectors installed between the interior and exterior sections of the cabinet.

3.2.13.3 Exception construction shall be routed in plenums and low velocity air passages. Routing in sections of the air passages with design air velocities in excess of 1000 LFPM shall not be allowed.

3.2.13.4 Exception construction shall be designed to minimize the build-up of contamination. Wiring to the fan motor shall either be via a power cord to a sealed junction box or by insulated spade connectors located near the fan(s). Individual wires from the control center to the fan(s) shall not be allowed. The connection shall be located no more than one foot from the fan/motor assembly. The wiring from the junction box or spade connectors to the fan motor shall be insulated cable or individual shielded wires sealed in an insulated tube. Wiring inside air plenums shall be secured against movement using plastic ties and tie downs or welded studs and bolted wire clamps.

3.2.14 Service Control Center: Light Canopy

3.2.14.1 All electrical components that service the motor, lamps, and outlets shall be located in a removable, uncontaminated electrical compartment. Accessories, lamp ballasts, fluorescent lamps, pressure gauges, switches, relays, motor controller(s), motor capacitor, warning alarms, etc. shall be housed in a service control center located on the front of the cabinet. The service control center shall be in a separate, ventilated compartment, which is connected to the main structure of the cabinet. On all units, the service control center connections to the cabinet interior shall be fitted with male/female plugs for disconnection, without having to enter a contaminated area, to facilitate moving and servicing of the cabinet. All of the penetrations into the cabinet air plenum systems shall meet the leak tightness requirements, Paragraph 3.11. The service control center shall be front access with no less than two stainless steel fasteners as the attachment unit. Wires, tubing, cables, conduits, pipes, etc. shall not be located or routed within the air passages of the cabinet, except as detailed in sections 3.2.13. The service control center shall be electrically and mechanically demountable to allow for rapid cabinet servicing. A cleanable, low voltage membrane touch pad shall be used to allow operators access to the cabinet functions.

3.2.14.2 The service control center, when in the operating position, shall be designed so that no objects can be placed on the canopy housing and remain there by gravity. The canopy shall not have the appearance of being a shelf.

3.2.14.3 If the control center contains non-electronic ballasts, the compartment shall be provided with at least a 40 percent open area to facilitate heat removal from ballasts and motor controllers for non-electronic ballasts. The build-up of temperatures above 150 degrees Fahrenheit inside the control center, six inches from any ballast during continuous operation of the fluorescent or ultraviolet lamps and cabinet motor(s) at an ambient temperature of 70 degrees Fahrenheit, shall result in corrective repairs as detailed in paragraph 6.3.4.

3.2.14.4 If the service control center drops open following the removal of any fasteners, the service control center shall be provided with a left and right removable cable support in the open position to secure the assembly during service activities and allow easy removal of the service control center.

3.2.14.5 The service control center wiring shall be organized systematically using terminal blocks and insulated C clip wiring terminals. All loose wiring shall be secured using plastic ties and tie downs. All



removable electrical components shall be mechanically attached. Soldered connections are prohibited on removable connections.

3.2.14.6 Service Control Center Access: The service control center should be provided with a perforated metal cover over the wiring section, fluorescent lamp assemblies excluded. This is to protect electrical components from damage during filter and blower repair and to prevent laboratory materials from accessing the interior and causing an electrical hazard. A cover shall be provided over the VFD and shall extend above and below the main chassis that the remaining cabinet wiring is attached to. Approved Alternative: If the top of the control center is recessed by protected dress panels the protective metal cover is waived.

3.2.14.7 The service control center shall be accessible using one inch thumb screws with a pilot end. No tools shall be required, except during shipment to secure the control center from shipping damages.

3.2.14.8 Wiring Harness Protection: Required Protection of Wiring harnesses from the service center into

Bulkhead connectors: Wiring penetrations shall be double insulated to protect individual wires from shipping and flexing of the harness during repeated openings of the canopy. Sharp edges from sheet metal shall be protected from cutting wiring in all wiring harnesses.

3.2.14.9 The cabinet power cord shall be directly connected to the service control center and secured with at least one faster on the top assembly panel using a plastic C-clip. The power cord shall be 14 feet in length and be equipped with a hospital grade plug.

3.2.14.10 PC board low voltage shall be provided with touch pad controls for the fluorescent, blower, UV and receptacle. Rocker switches are prohibited. The PC board shall have pilot lamp display on all touch pad low voltage switches and LED lamps on the PC board to trouble shoot the board itself for fluorescent, blower, UV and receptacle. There shall be a plug in diagram next to the PC board detailing the wiring harness plug in areas on the board. The board shall be attached using welded studs or tack on plastic stays.

3.2.14.11 Touch pad switches shall be raised to facilitate user ergonomics. Touch pad switches shall operate smoothly and require less than 0.5 pound force to activate or deactivate the fluorescent lamp, blower, UV or receptacle. Touch pad switches shall be easily cleaned and not be affected by decontamination chemical agents commonly used in biological research.

3.2.15 The exhaust connection for the four and six foot cabinets used to connect to the house exhaust system shall be a bolted rectangular exhaust transition provided with a 12-inch exhaust damper (4, 6 foot models) and exhaust cover panel. The exhaust transition shall be no more than 16 inches (+2”,-2”) in height, which includes the damper.

3.3 Dimensions

3.3.1 Overall Dimensions: Overall dimensions shall include all protrusions through and/or from the outside walls of the cabinet such as pipe nipples, side door assemblies, electrical connection boxes, cabinet seams, etc. Sample figures with dimension requirements are included in Appendix B.

3.3.1.1 Four foot, Class II, Type B2-Hard Connection

3.3.1.1.1 Overall width 54 inches (±1")

3.3.1.1.2 Overall Depth 34 inches (±1")

3.3.1.1.3 Overall Height - Bench with support frame, exhaust transition 92 inches (-4", +5")

3.3.1.1.4 Overall Height - Bench with exhaust transition 62 inches (±1")

3.3.1.2 Six foot, Class II, Type B2-Hard Connection

3.3.1.2.1 Overall Length 78 inches (±1")

3.3.1.2 2 Overall Depth 34 inches (±1")

3.3.1.2.3 Overall Height - Bench with support frame, exhaust transition 92 inches (-4", +5")

3.3.1.2.4 Overall Height - Bench with exhaust transition 62 inches (±1")

3.3.2 Inner Dimensions of Work Area

3.3.2.1 Four foot, Class II, Type B2 –Hard Connection



3.3.2.1.1 Inside wall to inside wall width shall be 46 inches, (±1")

3.3.2.1.2 Outer side wall to inner side wall width shall be no more than 4”

3.3.2.1.3 Depth (front edge of airfoil to back wall) shall be 25 inches (±1")

3.3.2.1.4 Height (work surface to diffuser) shall be 28 inches (±1")

3.3.2.2 Six foot, Class II, Type B2-Hard Connection

3.3.2.2.1 Inside wall to inside wall length shall be 70 inches, (±1")

3.3.2.2.2 Outer side wall to inner side wall width shall be no more than 4”

3.3.2.2.3 Depth (front edge of airfoil to back wall) shall be 25 inches (±1")

3.3.2.2.4 Height (work surface to diffuser) shall be 28 inches (±1")

3.4 Assembly

3.4.1 Door Way Requirement: The cabinet shall be capable of being moved through a doorway nominally 6'7" high, 2'11" wide using only equipment commonly available for furniture moving such as jacks and dollies, and it shall not be turned over on end. Cabinets assembled from upper and lower sections connected by joints with gaskets shall be acceptable. Cabinets having a separate supporting base shall be acceptable and the supporting base shall be included in the overall height listed in Paragraphs 3.3.1.1.4 and 3.3.1.2.4.

3.4.2 Bolted Cabinet Sections: For those cabinets with upper and lower sections of two or more sections, cabinets shall be bolted together to form a structural unit. Screw connection is prohibited. The supporting frame is considered the bolted unit in this specification.

3.4.3 Cabinet Sash Requirements: The bench and bench with channel stand cabinet window shall be in a metal shielded frame with gasket and slide mechanism. The window upper and lower horizontal edges shall be mounted in a 24 gauge type 304 stainless steel channel to provide protection from the loading and unloading of work materials and for certifier safety. Side edges shall be protected in a stainless metal channel which is secured to the glass permanently using adhesive. The glass shall be sealed into the frame with a flexible material to prevent window breakage. The horizontal width of the window frame, channel and glass thickness shall not exceed 0.35 inch. Approved alternative: the left and right bottom corners of the safety glass may be exposed provided the corners are half inch radius construction and beveled with no sharp edges. The exposed glass edges shall not be used as a stop against a metal assembly with rubber stops.

3.4.3.1 The window assembly shall have positive safety supports to hold the window open during loading and unloading.

3.4.3.2 The method for raising and lowering of the window frame shall be with the assistance of counter balance mechanisms located on the left and right side of the window frame. The mechanisms shall apply sufficient force to maintain the window frame in an open position and hold the window in the desired stop position. Approved alternative: the supplier shall provide a counter balance assembly using counter balance weights, with adjustable cables to ensure that the window remains level.

3.4.3.2.1 Upper and lower sash stops shall be provided with rubber bumpers to protect the sash assembly from an impact load of 10 pounds per side in either the raised or lowered position without any visible distortion in the stop assemblies or the related fasteners. Rubber stops shall contact the metal window frame, not glass or metal edges. All metal slides shall be protected from metal to metal contact with Teflon strips applied on all contact surfaces.

3.4.3.2.2 The manufacturer shall run window sash open and close cycle studies of at least 20,000 cycles to verify the integrity of the counter balance mechanisms for the application.

3.4.3.2.3 The supplier shall provide a sash mechanism to facilitate raising and lowering the sash with one hand. This is necessary because cabinet operators will use one hand to raise the sash while holding a research object with the other. Therefore the supplier shall provide a window assembly that can be raised and lowered with one hand within an offset of +/-10 inches from the centerline of the sash.

3.4.3.3 The slide mechanism shall consist of a flexible cable, stainless fasteners and lifters with stainless steel rails to allow the window to be raised and lowered with no more than 0.125 inch of lateral or transverse movement between the window frame and rails. Essentially the window shall not rack more than 0.375



inches from left to right upon raising and lowering. Racking is a manufacturer defect that will result in a 1.2.1 notification since the condition of racking generally results in window breakage as the operator applies more pressure in order to lift and close the sash. It is understood that if the sash is found out of adjustment that the university agent shall determine whether this is a manufacturing problem or maintenance. The cables should fasten to a lateral frame member of the window with stainless steel hooks to facilitate maintenance. Each lifting cable shall be provided with a counter balance mechanism designed to allow the window to be passively raised and lowered. If the window moves actively against or with gravitational forces, the manufacturer will be contacted to arrange for replacement parts to be installed at the manufacturer's expense, or a 1.2.1 notification will be made.

3.4.3.3.1 Lower Sash Stop: the cabinet shall be provided with two lower window stops and rubber bumpers. The bumpers shall contact only the metal frame of the sash.

3.4.3.3.2 The sliding window frame shall be provided with upper linear scavenger slots to prevent the ingress of room air into the clean zone

3.4.3.3.3 Sliding Window Exhaust Slots: The interior upper side of the sliding window frame shall be maintained in a negative pressure condition relative to the exterior atmosphere of the cabinet. This shall be accomplished with at least two ventilation slots per side on both the left and right sides of the window. The vents shall directly vent into the negative pressure plenum of the cabinet. The vents shall be punched and be tear proof to laboratory gloves, Paragraph 3.5.2.4.2.

3.4.3.3.4 Sash Handles: Two stainless steel handles shall be provided the lower outside edge of the window frame at the tri-sectional midpoints of the lateral window frame member. Window handles shall be permanently bonded to the window glass using adhesive or welded. In the event that the handles fail within a five year period from the date of manufacture, the manufacturer shall provide replacement parts and adhesive at no charge.

3.4.3.3.4.1 Each handle shall extend away from the outer lower edge of the window frame at least one inch.

3.4.3.3.4.2 The portion of each handle that projects away from the window frame shall be at least 14 gauge polished stainless steel with all corners and edges beveled to at least 0.0625 inch radius in both vertical and horizontal corners.

3.4.3.3.4.3 No holes and/or slots shall be allowed in the handle used by a cabinet operator to raise and lower the window, except for the attachment fasteners.

3.4.3.3.4.4 If the handles are attached with studs, cap nuts, split lock washers and flat washers shall be fabricated such that all exterior surfaces have no metal burrs.

3.4.3.3.4.5 SASH ALARM The operational height of the sliding window shall be set by the supplier with an audible warning device installed to sound when the window is raised above or lowered below the operating height. The alarm shall shut off when the sash is at the lowest point of travel (closed). The noise level of the alarm shall not exceed 80 db-A with a background sound level of less than 55-dbA. The sash alarm switch shall be low voltage 24V or less to reduce the risk of electric shock hazard to certifiers and operators.

3.4.3.3.4.6 Energy Saving Mode: The cabinet shall have an energy saving mode which shall be activated automatically when the view screen is placed in the closed position. When the cabinet is in the energy saving mode, the lights shall be off, the motor shall operate at a reduced speed, and the total cabinet current draw shall be no more than 2.0 amps. The cabinet manufacturer shall provide passing results of testing the cabinet to the NSF 49 Standard for both the Personnel and Product Protection with the cabinet view screen closed and the cabinet in energy saving mode. The three inch cylinder required in the NSF test may be omitted because the view screen has to be closed.

3.4.3.3.4.7 Option - UV Lamp Assembly: In order to protect laboratory personnel from reflected UV radiance, the cabinet window shall be capable of being lowered to the closed position with the cabinet blower left on for work zone sterility maintenance. The supplier shall insure that the cabinet blower cannot overheat in this operating configuration by allowing bypass air around the window and armrest with the lab air temperature not to exceed 80 degree F.

3.4.3.3.4.7.1 UV Interlock: The manufacturer shall provide a UV lamp interlock, which will prohibit the UV lamp from operating unless the sash is fully closed.

3.4.3.3.5 The window and frame shall be flat within 0.125-inch maximum offset in 70 inches. Windows, which do not conform to this requirement, will be replaced at the manufacturer's expense.



3.4.3.4 Unobstructed Work zone Interior Access Requirement: With the window in the raised position, access to the interior of the safety cabinet shall be unobstructed by cross members.

3.4.3.5 With the window frame removed, the mating face of the cabinet, which forms the frame that the window frame seals against, shall be flat (0.125-inch offset in 80 inches) and smooth so that a plastic shield can be taped in place temporarily to close the front opening of the cabinet during the leak tightness test, Paragraph 4.2.1, and for decontamination. An unobstructed frame clear space of at least 1.0-inch is recommended; a 0.75-inch absolute minimum shall be provided around the entire perimeter of the cabinet face with the window frame removed.

3.4.4 Operational Sash Height: The work access opening height shall be 8 inches (±0.25 inches) and a maximum open height of 20 inches ±1 inch for equipment loading and cleaning.

3.4.4.1 The removable solid work tray surface height from floor shall be 33 (±3) inches.

3.4.4.2 Work zone elevation shall be adjustable if cabinet base is provided: On bench cabinets, removable solid work tray surface height from floor shall be 33 inches.

3.4.4.3 The sash shall be slanted at an angle of 10 degrees from vertical ±2 degrees.

3.4.4.4 Air Curtain: The area directly behind the sash shall be provided with a velocity air wash which is at least twice the air velocity of the work zone to act as an air barrier to cleanse materials entering and leaving the work zone. The width of the air wash shall be determined by the manufacturer based on performance envelope studies.

3.4.4.5 Option: Microscope: The supplier shall offer a custom microscope sash option. This may entail retesting a safety cabinet performance for personnel and product protection. In certain instances a hinged view screen may be required.

3.5 Materials and Components

3.5.1 Corrosion and Moisture Resistant Requirement: All cabinet surfaces, interior and exterior shall be constructed of, or finished with, materials which are corrosion, flame and moisture resistant. This shall include all components of the cabinet, including but not limited to, motor(s), wiring, and other electrical components. These materials shall not deteriorate under exposure to liquid or vapor phase decontaminates such as formaldehyde, alcohol, chlorine dioxide iodophors, peracetic acid, halogenated phenols and hypochlorite solutions or exposure to ultraviolet radiance.

3.5.1.1 Option: Hydrogen Peroxide: The University shall reserve the right to specify cabinet construction to be used with hydrogen peroxide as a decontamination agent. The supplier at the time of request for purchase shall be notified that this option is being exercised and the supplier will then supply a cost estimate to comply with this additional requirement. This is a REMOTE possibility and therefore has not been included as a base bid optional request. The cost estimate shall be provided to the UW Purchasing agent for evaluation in conjunction with the University Agent and/or Biosafety Department.

3.5.2 Sheet Metal and Finishes

3.5.2.1 The thickness of metals used for cabinet work zone liner construction shall not be less than U.S.S. Gauge No. 16. The work surface construction shall not be less than U.S.S. Gauge No. 16. The exterior cabinet construction shall not be less than U.S.S. Gauge No.16 gauge steel on rigid/reinforced panel construction and 18 gauge on dress panels. Dress panels shall have positive gravity assist tabs or fasteners to prevent panels from harming operators or certifiers as a fall hazard.

3.5.2.1.1 Flexible or non-rigid positive pressure plenums are prohibited.

3.5.2.1.2 The supply filter plenum shall be designed to provide uniform airflow to the supply filter within a ten percent tolerance or less between row readings. The greatest care shall be used to select the interior design of the plenum using computational fluid dynamics, CFD, to determine the most efficient airflow path with the minimum energy requirement. Furthermore, the roughness of the plenum surfaces must be smooth and free of fabrication debris. Fabrication debris shall be defined as detached particles such as metal filings and grit greater than 0.0625 inch in size.

3.5.2.2 All cabinet interior work surfaces, including the drain trough assembly, shall be fabricated with corrosion resistant 16 gauge stainless steel that shall conform to Federal Specification QQ-S-766, Class 304, No. 4 satin finish. The window frame shall be stainless steel with at least a No. 4 satin finish. Raised surfaces on the liner walls as determined by inspection of the University Agent are a manufacturing defect. The manufacturer shall be informed to correct this on future models and if the



raised surfaces continue in later delivered models, the manufacturer shall be requested to replace the surface under examination or replace the cabinet.

3.5.2.2.1 The interior vertical walls of the stainless steel work zone shall be fabricated from a single sheet of stainless steel with punched scavenger slots at the work zone vertical walls, at the zone behind the sash, and for the frontal service entrance to the UV lamp sockets.

3.5.2.2.2 The right and left sides of the work zone intake shall be provided with at least ten scavenger slots per side to reduce the turbulence as the air stream turns and enters the front intake zone.

3.5.2.2.3 The right and left rear vertical corners of the work zone shall be provided with at least 0.4375-inch (±0.0625) radii corners.

3.5.2.3 Steel sheet used in the cabinet exterior fabrication shall be prime grade, cold rolled carbon steel. Carbon steel sheet shall be used where Paragraph 3.5.2.2 does not apply.

3.5.2.4 Carbon Steel Finish Before painting, surfaces shall be cleaned of dirt, oil, and grease. The carbon steel shall be given a phosphate coating treatment in accordance with Federal Specification TT-C-490. All welded studs shall be covered with rubber caps to protect the stud threads from over spray. Failure to cover the threads will result in a 1.2.1 notification and possible field repairs being carried out under the specified conditions of paragraph 6.3.4.

3.5.2.4.1 Painted Surfaces: Epoxy powder coated finish – painted permawhite shall be a uniform satin luster white or approved alternate. Prime and finish coats shall be applied by spraying or dipping, and baked after each coat for a minimum of 15 minutes at 300 degrees Fahrenheit. A total thickness of not less than one mil shall be achieved. Concealed surfaces or hollow metal sections shall be protected by the finish previously specified, by a suitable method, after welding, but before assembly. Epoxy coatings can be used to coat all carbon steel surfaces and they shall conform to TT-C-001224 and shall be a uniform satin luster white. A polyurethane coating can be used to coat all carbon steel surfaces and it shall conform to TT-C-001227 and shall be a uniform satin luster white. All carbon steel surfaces shall be painted and stainless surfaces and accessories protected from over spray. Painted over spray on cabinet surfaces shall result in a 1.2.1 notification and paragraph 6.3.4 will be exercised to remove this paint if possible.

3.5.2.4.1.1 Finish Color: White The cabinet will be painted white unless for individual purchase the color is specified at a pre-agreed on additional cost. The supplier shall provide the UW Purchasing Department a cost estimate for the color requested in the individual order.

3.5.2.4.1.2 Approved Alternate: Perma White powder coat is accepted under the terms of the contract.

3.5.2.4.2 Puncture and Tear Requirement: All exterior metal plates, window sash handles and frames, all interior work zone metal surfaces, all metal diffusers, the metal spill trough, all interior metal surfaces within six inches of each HEPA filter, and the fan/motor assembly and compartment shall have no exposed metal edges, seams or corners that will puncture or tear a nitrile examining glove when advanced across these surfaces with a maximum force not to exceed one pound per square inch of glove surface area. The nitrile glove cited in this requirement shall be Fisher Scientific brand, number 19-050-550C, marketed by Fisher Scientific 2000 Park Lane Drive, Pittsburgh, Pa 1-800-772-6733. Puncture and tears will be deemed as poor workmanship and result in a 1.2.1 notification and field repair as specified in paragraph 6.4.3. More than five areas in the cabinet having tear and puncture will result in cabinet rejection and request for a replacement.

3.5.2.4.3 Finish Texture Required: FLAT All exterior surfaces shall have a flat painted application. A flat finish surface shall be defined as a continuous surface in either the horizontal or vertical plane with no surface distortion in excess of ±.001 inch. Paint runs from either over spray or paint purposely designed with an irregular surface shall be a cause for rejection of the cabinet and result in a 1.2.1 notification.

3.5.2.4.4 Option: Reinforced Work Surface: The manufacturer shall offer a reinforced work surface designed for increments of 100 pound loading up to 300 pounds.

3.5.2.5 Supply Air Grill (SAG) and Air Curtain

3.5.2.5.1 The SAG shall be stainless steel and constructed in accordance with specifications cited in Paragraph 3.7.3. Fasteners used to hold the grill in place shall be tear resistant per Paragraph 3.5.2.4.2.

3.5.2.5.2 The SAG shall be mounted approximately 2.0 to 4.0 inches below the filter media. The SAG shall



fit flush to the left and right liner sidewalls and rear wall.

3.5.2.5.3 Air Curtain: The front SAG edge shall be turned to allow for an air curtain on the interior side of the sash to control ingress of room particulates during operator movement into and out of the cabinet work zone.

3.5.2.5.4 The SAG shall be easily removed from the attachment points with holes in the SAG at least 40 percent larger than the fastener shaft to facilitate SAG installation. SAG removal that results in damage to the cabinet sidewalls or filter will result in a 1.2.1 notification as a manufacturing defect. The manufacturer will be requested to supply a new SAG, fasteners, re-drill thumb screw holes or replace the safety cabinet.

3.5.2.5.5 No open cell foam shall be allowed on the SAG or within the vicinity of the cabinet work zone (work surface to downstream face of the supply HEPA filter).

3.5.2.5.6 The space between the SAG and HEPA filter shall be a smooth, stainless side wall contiguous with the filter gasket. No cavities or structural reinforcement in this critical area shall be allowed, due to the necessity for field filter repairs and prevention of contamination.

3.5.2.5.7 All edges on the SAG shall be smooth and will not tear or puncture a laboratory nitrile glove as detailed in paragraph 3.5.2.4.2.

3.5.2.6 External Air Balancing Damper

3.5.2.6.1 The cabinet shall contain an external leak tight air balancing damper located after the uncontaminated exhaust pressure plenum, just above the exhaust outlet collar. The damper shall be adjustable with a locking device and have a large rotating arm for field maintenance activities.

3.5.2.6.2 The damper assembly shall be constructed of stainless steel, have an o-ring seal, and pass a leak test.

3.5.2.6.3 The damper assembly shall be of the same finish as the interior liner. Non-stainless steel dampers are prohibited and will result in a 1.2.1 notification. The damper assembly shall be smooth and pass the tear resistance test per 3.5.2.4.2.

3.5.2.7 External Transition and Airflow Monitor

3.5.2.7.1 Manufacturer shall supply a hard connected transition with a 12 inch round exhaust collar. The external leak tight damper assembly shall be 12 inch diameter (±0.25). Smaller sizes shall be considered on an individual basis; however the 12 inch diameter damper shall be standard.

3.5.2.7.2 Manufacturer shall supply stainless hose rings and reinforced flex duct to allow contractors to connect the damper assembly to the cabinet and to the house exhaust duct without the use of fasteners which can damage the damper o-ring seal. The manufacturer shall provide written instructions and drawing with each cabinet to explain to cabinet installers not to use fasteners on the stainless damper assembly and to use the o-rings and flex seal. If the manufacturer uses companion angle rings and gasket to connect the upstream isolation damper to the exhaust transition, hose rings shall be used on the downstream section of the damper assembly.

3.5.2.7.3 Airflow Monitor and Interlock: An airflow monitor is required on a Class II Type B2 cabinet. The airflow monitor shall be interlocked to the cabinet blower switch and not allow operation of the cabinet blower unless the building exhaust has been proven. Further, the interlock shall shut off the cabinet blower and turn on an alarm if the building exhaust decreases 20% or more, or fails completely. The airflow monitor shall be field adjustable.

3.5.3 Glass

3.5.3.1 All windows shall be 0.25 inch laminated safety plate glass in conformance with PPG's "Duo-Lite" or equal or 0.25-inch tempered glass in conformance with Federal Specification DD-G-1403. All glass shall conform to ANSI Z97.1-2009.

3.5.3.2 Sash Breakage: With the sash in the lower position, the manufacturer shall warrantee the sash against breakage for up to three inches static pressure. If this cannot be warranted then a sign shall be installed warning operators not to close the sash and the UV lamp lower switch relocated to that position that allows airflow and maintains the lab air balance.

3.5.3.3 Exposed glass edges are prohibited except at corners. All exposed corners shall be 0.375 inch to 0.50 inch radius to prevent sharp exposed corners. All edges shall be metal clad, glass edges beveled and corners rounded to at least 0.375 inch radius.



3.5.3.4 Sash glass shall not exceed ¼” thickness.

3.5.4 Filters

3.5.4.1 High Efficiency Particulate Air (HEPA) filters zero probed, 99.99 percent efficiency, shall be required for both supply and exhaust air systems of all cabinets. Refer to USAEC, ORNL-NSIC-65 or AG-1 Code on Nuclear Air and Gas Treatment. The University may require higher efficiency filters ULPA (greater than 99.99 percent) on an individual procurement basis.

3.5.4.1.1 Compliance with Paragraph 3.5.4.4 is compliance with paragraph 3.5.4.1. All filters shall be at least 99.99% rated by scan test.

3.5.4.2 The cabinet shall be designed such that all HEPA filters can be replaced without any additional attachments or assemblies permanently affixed to the HEPA filter frames. Failure to conform to this requirement shall result in a 1.2.1 notification.

3.5.4.3 All supply and exhaust filters should be high efficiency, fire resistant, particulate air filters, in conformance with MIL-F-51079B, Type 1C with fire retardant treated plywood or aluminum frames, self-extinguishing type adhesives and sealant, and glass fiber media; no filter shall have factory test patching greater than one percent of the open face area. Alternatively refer to AG-1 Code on Nuclear Air and Gas Treatment. Filter sizes not listed in MIL-F-51079B shall be acceptable. Paragraph 3.4.7 of the MIL-F-51079B covering the environmental testing shall not apply.

3.5.4.3.1 Compliance with Paragraph 3.5.4.4 is compliance with paragraph 3.5.4.3. All filters shall be made with at least fire retardant materials.

3.5.4.4 All supply and exhaust filters shall conform to IEST Standard IEST-RP-CC-001.5 Each filter shall be construction Grade 3 or 4, performance Type C or J. A label shall be affixed to each filter frame detailing testing requirements, ratings, manufacturer’s test results, date tested, and name of the factory test agent. The test results shall indicate DOP leak rate, airflow in CFM and associated pressure drop across the filter during testing.

3.5.4.5 Filter mounting(s) tolerances shall be for openings up to 20 inches: +0.0625, -0 inch, and for openings over 20 inches: +0.125, -0 inch. The squareness of the filter mountings shall have diagonals within 0.0625 inch total allowance. Width of the gasket seating area shall be 0.75 inch (+0.25, -0 inch).

3.5.4.5.1 Filter mountings, which provide a knife-edge seal between the face of the filter and the sealing edge of the frame are prohibited and will result in purchase cancellation unless the gel seal is used on filters located beneath the worksurface. If gel seal is used, it shall be silicone base, “Blue Gel”, the integrity of which as defined by the aerosol leak test (0.01% scan or 0.005% probe) for the life of the filter. Other gel seals are prohibited. This includes polyurethane.

3.5.4.6 HEPA filters mounted in such a manner that the total weight of the filter(s) is supported by the clamping mechanisms shall not be acceptable. Exhaust HEPA filters may be mounted in a manner such that the total weight of the filter(s) can be supported by the clamping mechanism provided that the clamps are designed to carry the filter weight and uniformly compress the filter gasket(s) with at least 10 pounds force per lineal inch of gasket material.

3.5.4.7 The factory certification report shall detail the filter manufacturer, filter model, filter rated airflow and filter static pressure at the design airflow and the filter serial number and dimensions of each filter.

. 3.5.5 Gaskets, Seals and Clamps

3.5.5.1 HEPA filter seals using fluid seal systems are not acceptable and will result in a 1.2.1 notification.

3.5.5.2 The HEPA filter gasket materials shall be cellular sheet or molded rubber or closed cell expanded neoprene gasket materials.

3.5.5.2.1 Constant Pressure Clamps: Only clamps with internal springs to provide a constant load to the clamping frame shall be allowed. Approved Alternative: If a telescoping steel plenum is provided, a screw clamp system which evenly distributes the clamping force to the filter frames shall be allowed.

3.5.5.2.2 All HEPA filters shall be clamped to the cabinet frame. Clamps shall be designed with sufficient rigidity to compress the filter gasket material. The method of choice for filter clamps will be constant pressure spring type filter clamps used to clamp supply and exhaust HEPA filters in place. The clamping mechanism should be designed to apply a minimum pressure necessary to uniformly compress the gasket depth 50%.



3.5.5.2.3 The supplier shall provide clearances inside the cabinet filter plenums to access the clamps for filter service. In accessible clamps will result in a 1.2.1 notification.

3.5.5.3 Unless specified herein, neoprene rubber gasketing, 25 to 45 Durometer, with vulcanized or sealed joints shall be used in all non-welded cabinet joints, seams, and on the facing of all air access panels of the cabinet which form a barrier to the contaminated air plenums. The structural strength of the joints and connections of the service panels shall be independent of the seal produced by the gasket.

3.5.5.3.1 Gaskets shall have a minimum width of 0.50 inch and be a minimum of 0.25 inch thick on all joints and panels, which form a barrier between contaminated areas of the cabinet and ambient environment. Gaskets shall deform at least 50% percent in thickness from the perpendicular force applied from the fastening unit. Filter gaskets shall be by the filter manufacturer.

3.5.5.3.2 The use of open cell gaskets and/or sheeting shall not be acceptable in the air passages of the cabinet plenum systems.

3.5.5.4 All joints or assemblies made with sealant bonding in non-contaminated areas shall be made by one of the following methods. The structural strength of the joints or assemblies shall be independent of sealant.

3.5.5.4.1 Two part accelerated synthetic rubber (polysulfide type) sealing compound, temperature resistant, high adhesion aircraft specification grade, MIL-S-8802 or PR-1422 Class B-2, Products Research Company or equal shall be acceptable.

3.5.5.4.2 One part silicone base sealant compound, such as Dow Corning RTV 732 Adhesive Sealant, Dow Corning RTV 781 Building Sealant or equal shall be acceptable.

3.5.5.5 All joints formed using fasteners and gaskets and made to conform to leak tight construction (Paragraph 3.11) shall not contain sealant bonding on the fasteners. Sealant bonding on the fasteners shall be deemed a fabrication defect and result in a 2.1.1 notice of noncompliance.

3.5.6 Fan(s)

3.5.6.1 The cabinet shall have no more than one motor driven fan system for supply air. The fan/motor set shall rest by gravity on the cabinet attaching plenum. Fan/motor set suspended by attachment assemblies are prohibited. Fan/motor suspended by bonded rubber springs are prohibited and considered a fall and maintenance hazard. If the fan being turned off and the sash closed results in reverse fan flow or laboratory over pressurization, an accessory sign shall be provided in the manual informing operators not to routinely close the sash if the building ventilation is run 24/7. The sign shall be 1/2 inch Arial letters and read: Close the sash to stop only in emergency conditions. When operations are completed, decontaminate the area and lower the sash to two inch open.”

3.5.6.1.1 No motor driven fan system shall be provided to move the exhaust air.

3.5.6.2 The fan shall be labeled in accordance with Bulletin 211 of the Air Moving and Conditioning Association. Manufacturer's name and nameplate data shall be labeled on each fan scroll. Fan housing and blades shall be fabricated of or protected with corrosion resistant materials to withstand normal laboratory or chemical fumes and as specified in Paragraph 3.5.1.

3.5.6.3 Total fan delivery shall fall off no more than 10 percent as a result of 100 percent increase in the pressure drop across the filters or filter static pressure increase of 0.3-inches water column whichever is greater. The supplier shall provide a graph and data table of the test results for the automatic airflow compensation for filter loading from 0 to 200 percent. No manual adjustment shall be required to compensate for filter loading.

3.5.6.4 The fan shall be direct connected forward curved centrifugal fan in conformance with AMCA standards.

3.5.6.5 The fan installed shall be balanced within the vibration limits of Section 3.10.

3.5.6.6 The fan/motor assembly shall be isolated from the cabinet by means of a vibration isolation system such as motor struts and/or bonded rubber rings. Air connections to the fan should have a gasket interface to reduce vibration transmission. Flexible plenums, including those made out of fabric, are prohibited. Attachment screws and associated metal materials shall be stainless steel.

3.5.6.6.1 The fan/connection to the cabinet shall be such that air leakage is less than 0.5 percent of the total airflow. The fan connection to the filter plenum shall be with one slide rail from the fan scroll to the plenum using one clamp bolt. All fasteners should be stainless steel.



3.5.6.6.2 The cabinet fan/motor may be separately isolated from the fan scroll, provided that rubber isolators are used directly between the motor attachment assembly and the fan scroll. Under no circumstances shall the rubber isolators be used to vertically isolate the fan motor from the fan wheel and scroll. All blowers shall rest on the cabinet interior such that the blower can be unbolted without having to support the blower during removal. Suspended blowers will result in purchase cancellation.

3.5.6.6.3 No flexible fan sleeve can be used in place of a sealed metal positive pressure filter plenum.

3.5.6.7 The fan wheel shall contain provisions for attaching a wheel puller in the event that the fan and motor must be separated for maintenance.

3.5.6.8 The fan wheel and scroll shall be designed to be separated without the need to cut welds in the scroll.

3.5.6.9 The fan assembly shall rest by gravity on the cabinet sub-assembly. Fan assemblies, which have been suspended from an upper panel are prohibited and will result in purchase cancellation.

3.5.6.10 The fan scroll when installed shall not contact the exterior cabinet metal walls. A minimum peripheral clear space of 0.375 inch shall be maintained.

3.5.6.11 The fan wheel shall be balanced to meet the vibration requirements in this specification.

3.5.7 Electrical Components

3.5.7.1 Electrical Requirements - General

3.5.7.1.1 The cabinet electrical system, when connected to the owner supplied 115VAC/20Ampere all outlet, shall have a ground circuit resistance not to exceed 0.15 ohms, a “no fault” electrical leakage current tested to 3500 micro-amps single fault per UL61010-1, correct wiring polarity and ground fault circuit interrupter that will trip at 5.0 milli-amperes. If these minimal electrical conditions cannot be achieved, the manufacturer shall be contacted and the components repaired or replaced. This shall include all components contained under paragraph 3.5.7.

3.5.7.1.2 No polychlorinated biphenyls (PCBs) shall be allowed in any electrical component.

3.5.7.1.3 Cable and wire shall conform to Federal Specification Cable Power, Electrical and Wire (Weather Resistant), J-C-145.

3.5.7.1.4 Receptacles and plugs shall conform to Federal Specification W-C-00596.

3.5.7.1.5 All electrical components and wiring shall conform to the latest edition of the National Electrical Code and the National Electrical Manufacturer’s Association (NEMA). The entire cabinet shall be listed as certified by the Underwriters Laboratories or the Canadian Standards Association.

3.5.7.1.6 Separate metal parts of the cabinet shall be interconnected (jumpered) and grounded to the common ground with the electrical components. These interconnections shall be made on the inside of the cabinet.

3.5.7.1.7 Electrical parts shall not be located in the air passage from the work surface to the filters, except the fan motor, one junction box containing only permanently connected conductors, wiring for the optional UV lamp assembly, and necessary wiring and cables.

3.5.7.1.8 Cabinet Wiring Diagram location: Two up-to-date wiring diagrams showing connection of all electrical components shall be provided. One diagram permanently attached to the cabinet at the service control center and the other provided in the service manual. Approved alternative: If a dress panel is used to cover the blower plenum access panel, the permanent wiring diagram may be attached to a removable dress panel.

3.5.7.2 Main Power

3.5.7.2.1 Service plugs to electrically disconnect the Service Control Center: Male/female plastic plugs shall be provided to separate the service control center from the cabinet body. On the side of the plug going to the cabinet body shall be provided with a clamp assembly to secure the plug wiring harness. The wiring harness itself shall be provided with a stress relief mechanism to allow wiring to easily be secured back into the service control center when reattached to the cabinet body. The wiring harness shall not rub against the window, contact sharp sheet metal assemblies which can degrade insulation, or prevent closure of the service center assembly.



3.5.7.2.2 The cabinet flexible power cord shall be a three wire with ground wire for single phase power unless otherwise specified, and shall be sized in accordance with the National Electrical Code for the specified load. It shall be labeled in accordance with UL Standard(s) UL-817 and/or UL-62. The power cord should directly connect to the service control center and not pass through contaminated plenums.

3.5.7.2.3 Main Power Cord shall be number 12 gauge cord with a NEMA 5-20P cap plug. The exterior portion of the cord shall be at least 14 feet in length. The cap and cord shall be of monolithic construction. Separate cord cap plugs are prohibited. The cord shall be secured to the cabinet frame to maintain the cord in position and not obstruct other components of the cabinet.

3.5.7.2.4 The main power shall exit the top of the cabinet on the operator right side.

3.5.7.3 Motor

3.5.7.3.1 The motor used in the fan system shall be designed that the motor winding temperature shall not exceed a temperature of 105 degrees centigrade in a maximum ambient temperature of 48 degrees centigrade (120 degrees Fahrenheit) under any maximum load condition. The thermal protector shall not trip at 115 percent of the rated voltage under maximum load and ambient temperature conditions. The motor shall be rated for 24 hour continuous operation. Nuisance trips shall not be allowed. The motor shall conform to Federal Specification CC-M-636.

3.5.7.3.1.1 Motor capacitor shall contain no derivatives of polychlorinated biphenyls (PCBs).

3.5.7.3.1.2 Motor Electrical Service Entrance: The wiring to the fan motor should be provided with insulated removable electrical connectors to allow measurement of the operating motor voltage.

3.5.7.3.2 The motor manufacturer should rate the motor for a continuous running time of 50,000 hours (5.7 years).

3.5.7.3.3 The motor shall be sealed (not shielded) bearing construction.

3.5.7.3.4 The motor shall be sized to operate the fan at airflow sufficient to maintain airflow and face velocity in accordance with Paragraph 3.7 with a total fan delivery requirement as specified in Paragraph 3.5.6.3.

3.5.7.3.5 Electrical Requirements: Motor shall be equal to or less than 0.5 HP and shall be 230 volts, 3-phase, 60 hertz AC. Dual rated voltage motors shall not be acceptable. Full load is defined as that condition associated with the fan operating at a static pressure condition necessary to overcome at least a 100 percent increase in filter static pressure with up to a maximum of a 10 percent drop in total airflow in CFM, Paragraph 3.5.6.3.

3.5.7.3.6 The motor performance curve should be provided with each cabinet in the service manual with a format as detailed in Paragraph 3.1.1. Motor performance data shall be provided in the initial bid.

3.5.7.4 Motor Controller

3.5.7.4.1 Motor controllers shall be an adjustable variable frequency drive (VFD). The VFD shall be 115V AC single phase input 230V, three phase output capable of running a ½ hp motor. The VFD shall be programmed with an algorithm that automatically adjusts the motor control frequency/speed to compensate for changes in filter loading.

3.5.7.4.2 Motor controllers shall have a provision for reduced power operation when the cabinet sash is closed.

3.5.7.4.3 Motor controller switches/adjustment knobs shall be readily accessible if the motor controller can be adjusted manually by certifiers, under the direction of technical service representatives.

3.5.7.4.4 A cover shall be provided for the motor controller/VFD to protect it during repair work. The cover shall be vented and extend above and below the main chassis that the remaining wiring is attached to.

3.5.7.5 Lighting

3.5.7.5.1 Ballasts shall be UL and/or CSA approved. Each unit shall be designed to satisfactorily start and operate the type of fluorescent lamp in the particular fixture and shall meet the current practice and requirements of the "Certified Ballast Manufacturer." Ballast design voltage shall suit the circuit voltage from which the fixtures are to be operated. Two lamp ballasts for operation of rapid



start types of fluorescent lamps may be series sequence type. Ballasts shall be securely fastened in place with the mounting surface of the ballast making a complete contact with the surface of the ballast mounting plate of the fixture as practical.

3.5.7.5.2 No ballast(s) shall contain any derivative of polychlorinated biphenyls (PCBs).

3.5.7.5.3 Electromagnetic ballasts are prohibited in front mounted canopy control assemblies unless the assembly has at least 25 percent open area for ventilation.

3.5.7.6 Duplex Receptacles

3.5.7.6.1 A 115 volt, 60 hertz, AC, three prong convenience duplex receptacle for grounded plugs shall be provided on each side of the cabinet sidewalls (left and right) just above the work surface. Work tray removal and average user reach capabilities shall be considered. The receptacles as located shall cause minimal disruption of the airflow pattern and allow at least a 2.0 inch vertical clearance between the uppermost section of the work tray and the lower edge of the faceplate to the receptacle. All duplex outlets shall be Hubbell No. 5262 specification grade or equivalent. All receptacles shall be protected with a ground fault circuit interrupter system as defined in paragraph 3.5.7.6.2. One of the receptacles shall be a GFCI which will feed the second duplex receptacle on 4 and 6 foot cabinets. The GFCI should have a single waterproof cover and the standard duplex outlet shall have dual independent covers. Both outlets shall have a circuit breaker or fuse inside the service control center.

3.5.7.6.2 Ground Fault Circuit Interrupter (GFCI) A ground fault circuit interrupter shall be provided on one duplex outlet circuit and be designed for a maximum threshold leakage of five milli-amperes. The circuit shall be protected with a circuit breaker or fuse, which is separate from the protection device for the motor, lights, and control circuit, and shall comply with the National Electrical Code.

3.5.7.7 Controls

3.5.7.7.1 Switches shall conform to Federal Specifications W-S-893 and W-S-00896.

3.5.7.7.2 Touch Pad switches shall be low voltage membrane touch pad.

3.5.7.7.3 Touch pad switches shall be raised to facilitate user ergonomics. Touch pad switches shall operate smoothly and require little force to activate or deactivate the fluorescent lamp, blower, UV and receptacle. Touch pad switches shall be easily cleaned and not be affected by decontamination chemical agents commonly used in biological research.

3.5.7.7.4 PC Board low voltage shall be provided with touch pad controls for the fluorescent, blower, UV and receptacle. Rocker switches are prohibited. The PC board shall have pilot lamp display on all touch pad low voltage switches and LED lamps on the PC board to trouble shoot the board itself for the fluorescent, blower, UV, receptacle, and sash position sensor operations. There shall be a ladder schematic next to the PC board detailing the wiring harness plug in areas on the board. The board shall be attached using welded studs or tack on plastic stays.

3.5.8 Plumbing

3.5.8.1 Work Area Trough Drain Pipe Assembly

3.5.8.1.1 A stainless steel drainpipe shall be connected to the lowest point in the perimeter drain spillage trough, which shall be piped to a minimum 0.375 inch female NPT stainless steel ball valve located on the front left side of the cabinet. The ball valve and ball valve connection shall conform to leak tight construction, Paragraph 3.11. This shall be provided on all UW cabinets.

3.5.8.1.2 The handle of the drain valve and/or an appropriate area next to the drain valve shall be permanently marked DRAIN VALVE. The open and closed positions of the drain valve shall be permanently marked on the cabinet next to the drain valve or marked on the handle of the valve itself.

3.5.8.1.3 The drain handle, in the closed position, shall point away from the cabinet operator and be horizontal.

3.5.8.1.4 The welded stainless pipe nipple to connect the drain valve shall be located such that the installation of the valve assembly axle does not contact the tubular frame assembly or any surfaces of the cabinet. Valves striking the cabinet surfaces or installed such that the handle protrudes into the knee space is a safety hazard and the manufacturer shall receive a 1.2.1 notification requiring that the handle assembly and welded nipple be relocated to a location that does not interfere with cabinet operators or cause damage to cabinet surfaces.



3.5.8.2 Aerosol Sampling Port Assembly

3.5.8.2.1 An external aerosol port connected to the internal positive pressure plenum shall be provided upstream of the HEPA filter(s) for aerosol testing.

3.5.8.2.2 The port shall be located on the upper horizontal metal plate of the cabinet. The port shall be a welded coupling, 0.375-inch NPT female pipe size. The coupling shall be continuous welded to the cabinet metal plate. A 0.375 inch-NPT male pipe plug shall be used to seal the port entrance. The pipe plug and welded seam shall conform to leak tightness requirements as detailed in Paragraph 3.11. The pipe plug shall be stainless steel and the threads wrapped in Teflon tape.

3.5.8.2.3 The port connection to the internal positive pressure plenum shall be hard connected using either a welded coupling connection or a bulkhead connection. Flexible connectors to the positive pressure plenum are prohibited. The interconnection between the exterior connector and positive pressure plenum may either be flexible tubing such as tygon or pipe. Flexible tubing shall be secured to the connectors with clamps.

3.5.8.2.4 The port connection into the internal positive pressure plenum shall be located such that a uniformly mixed air sample can be withdrawn for testing. Uniformly mixed, shall mean that sample which produces no more than a 10 percent variance in 100 percent aerosol measurement when the aerosol is introduced into the cabinet work zone.

3.5.8.2.5 A warning label shall be prominently displayed near the plug advising that the cabinet be decontaminated prior to removing the plug.

3.5.8.3 Side Wall Hose Cock Assembly

3.5.8.3.1 Petcock Requirement: Four 0.375 inch IPS stainless steel nipples or couplings with inside pipe threads shall be installed and sealed into the end panels in the cabinet liner, two per panel. One panel shall be provided with two petcocks, marked gas and vacuum, and tapered hose connections. Internal piping for the petcocks shall be copper. Optional: Two optional petcock connections on the operator left side shall not be provided unless specified.

3.5.8.3.2 Connections in the sidewalls shall be located toward the rear of the work area and be stacked one above and recessed behind the other with the top connection approximately 4 to 12 inches off of the work surface.

3.5.8.3.3 Petcock Labels The lower hose connections shall be at least two inches above the uppermost section of the work tray to facilitate ease in removal of the work tray. The upper petcock assembly shall be marked for gas and the lower petcock assembly marked for vacuum.

3.5.8.3.4 The two unused couplings in the remaining left side wall shall be capped with permanent stainless steel pipe caps on the interior side and temporary exterior pipe caps (such plastic pipe plugs) on the exterior.

3.5.8.3.5 Petcocks shall be internally piped to the rear section of the lower horizontal panel of the work zone.

3.5.8.3.6 All exterior pipe connections for the petcocks shall be 3/8-inch brass pipe female with tube connectors.

3.5.8.3.7 Option: Supplier shall offer remote controlled valve option to all petcocks.

3.5.9 Pressure Gauges and Cabinet Operator Warning Systems

3.5.9.1 Pressure Gauges: Each Class II Type B2 cabinet shall be equipped with two front mounted diaphragm operated, mechanical differential pressure gauges rated for ±5 percent full scale accuracy. The gauges shall measure the differential pressure from the laboratory to the supply HEPA filter plenum and the exhaust HEPA filter negative pressure plenum. The gauge scale shall read at least 200 percent of the initial plenum pressure.

3.5.9.1.2 The HEPA filter gauges shall be a four inch diameter gauge as manufactured by Dwyer Instruments, Michigan City, Indiana or equivalent.

3.5.9.1.3 The gauges shall conform to Paragraph 3.11.

3.5.9.1.4 The gauges shall be mounted in a location that does not require gauge disassembly in order to remove HEPA filters.



3.5.9.1.5 The gauges shall have a front adjustable screw to adjust the needle to zero without disassembly of the gauge or removal of any cabinet assemblies to access this adjustment screw.

3.5.9.2 Airflow Monitoring System: Each Class II Type B2 cabinet shall be equipped with an airflow monitoring system downstream of the exhaust HEPA filter. The airflow monitoring system shall be connected to the cabinet operator warning system. The warning device shall be located in the front of the cabinet and be designed to alert cabinet operators when the mass exhaust airflow has dropped to or below 20 percent of the set point exhaust airflow for certification. The power source for the airflow monitor shall be located inside the service control center. The cabinet internal supply blower shall be interlocked to the airflow warning system and automatically turn off the supply blower when the system is in alarm condition. The airflow warning system shall not be operated from the cabinet blower switch and will remain on continuously. The airflow warning system shall be field calibrated by the certifier.

3.5.10 Leak Tight Damper in Exhaust Transition

3.5.10.1 Each cabinet shall be provided with a 12 inch diameter airtight damper to completely isolate the cabinet from the building exhaust system. The damper shall be a stainless steel butterfly assembly mounted in a seamless duct. The mating surface between the damper and duct shall be a continuous rubber ring. The damper shall be gasketed and provided with each cabinet. The damper shall be 12 inches in diameter.

3.5.10.2 The damper shall be operated with a handle which is connected to the pivoting shaft of the damper blade.

3.5.10.3 The end connectors of the damper shaft shall be gas tight and the damper arm shall be vertical when open, ±10 degrees.

3.5.10.4 All metal parts of the damper assembly shall be stainless steel.

3.5.10.5 The damper assembly shall conform to leak tight construction, Paragraph 3.11.

3.5.10.6 The damper assembly shall have a 0.5 inch diameter hole located on the front side of the damper assembly for testing and aerosol injection. The hole shall be finished smooth and be provided with a sealable plug.

3.5.10.7 The damper handle shall be capable of being locked into place by University of Wisconsin – Madison staff. This will ensure users are not tampering with the location of the damper and affecting airflow

3.6 Lighting and Germicidal Radiance

3.6.1 Light intensities shall average no less than 60 fc (foot-candles) on the work surface, where background light levels average a maximum of 15 fc. Test procedure shall follow NSF/ANSI 49-2012 listed procedure.

3.6.2 The light source shall be obscured from direct view of the operator and will not create any eye strain from glare on the sash glass.

3.6.3 The fluorescent lamps shall be cool white, rapid start type. Lamps shall be located outside the workspace and their reflection shall not interfere with visibility through the window.

3.6.3.1 The fluorescent lamp assembly shall be front access and located exterior to the work zone.

3.6.4 Option: Germicidal Lamp: A germicidal ultraviolet lamp is optional; the assembly shall be attached to the rear interior wall of the work zone. The lamp shall be located below the supply diffuser and not interfere with the installation and removal of the diffuser. The lamp shall be a low-pressure mercury vapor type, which emits approximately 90 percent of the non-ionizing radiance at 253.7 nanometers. The lamp, when tested, shall be designed to produce at least irradiance on the work surface of the cabinet of 100 µw/cm2 with no individual reading of less than 40 µw/cm2 in any area of the work surface, exclusive of those areas which are four inches from the work zone sidewalls. All interior work zone components shall be UV resistant.

3.6.4.1 The lamp bulb shall be a low ozone bulb.

3.6.4.2 The UV lamp holders shall be removable from within the biological safety cabinet work zone. UV lamp holders and starters that require dismantling of air plenums for parts replacement are prohibited and will result in a 1.2.1 notification.

3.6.4.3 The UV bulb shall be fabricated from quartz glass (hard glass). Soft glass UV lamps are prohibited.



3.6.4.4 The UV bulb shall be designed to operate with the cabinet window in the fully lowered position. The enabling switch logic will preclude UV operation in any other sash position to protect the operator from reflected UV radiance.

3.6.4.5 The manufacturer shall provide a method to shut off the UV lamp 15 minutes after the UV system has been activated. This can be done by time control relay or by a programmable circuit with touch pad LED panel. The operation time of the UV lamp shall be programmable in 15 minute or 1 hour increments through the control panel.

3.6.4.6 Test Protocol: UV; Take four measurements along the centerline of the work surface spaced 12 inches from the side walls. Allow the lamp to operate for one minute before taking readings. The minimum average reading shall be at least 100 µw/cm2.

3.7 Airflow

3.7.1 Down Flow: The downward airflow velocity through the cross section of the unobstructed work area shall be maintained at a level which has been verified by the manufacturer by performance envelope testing. This requirement shall have an average that must fall within ±5 percent of the design velocity. Any individual reading shall fall within ±20 percent of the design velocity as measured in the design horizontal plane. Listed NSF-49 downflow readings may be substituted for this paragraph and the NSF grid followed, however the manufacturer shall specify the grid horizontal and vertical locations in an affixed laminated label. If cabinet fixtures must be removed to measure the downflow velocity, the label will provide instructions to this effect.

3.7.1.1 The unobstructed work area shall be defined as the area formed by a horizontal plane projection with a perimeter boundary four inches from the work zone sidewalls, rear wall, and sash. NSF-49 grids may be substituted for this section.

3.7.2 Face Velocity

3.7.2.1 8 Inch Access Opening to Workspace The supply air through the front work access opening shall have a face velocity of 105 LFPM, ±5 LFPM. The face velocity shall be determined in accordance with Paragraph 4.2.4.

3.7.2.2 Achievement of the down flow and face velocities detailed in Paragraphs 3.7.1 and 3.7.2 shall be through the application of sound engineering practice in the design of the inlet airfoil, internal positive pressure plenum, fan discharge outlet to the positive pressure plenum, balancing damper, selection of fan/motor equipment, sizing of filters, and design of the supply air diffuser. Once the cabinet face velocity and down flow velocity have been determined, the performance of the selected fan/motor system shall conform to Paragraph 3.5.6.3 within the chosen face velocity limits and required down flow velocity limits according to test procedures in NSF49, Test Method. Under no circumstance will biological containment within a range of face velocities negate compliance with Paragraph 3.5.6.3.

3.7.3 Turbulence caused by filter sealing materials, diffusers, etc. shall not result in an upward vertical component or refluxing of airflow in the workspace. Solid phase CO2 in water, smoke pencils, MSA smoke sticks, and/or synthetic fog shall be used to check each operational cabinet for an upward vertical component or refluxing of airflow in the workspace due to obstructions. Refluxing of airflow caused by filter media case seals, filter cases, and filter frame supports shall be eliminated by use of a perforated stainless steel supply air diffuser (SAG) that is fabricated with close tolerance to the cabinet side walls, less than 1/8 inch spacing. Titanium tetrachloride smoke and zinc chloride smoke bombs are prohibited.

3.7.4 The quantity of air leaving the work space through the rear perforated area shall be fixed by the supplier and shall not be adjustable.

3.7.5 The cabinet air diffusers, filtration systems, stainless damper, plenums and fan/motor systems which will produce the required air velocities shall be designed to produce near uniform and constant velocity distributions on the downstream side of the supply and exhaust filter systems under undisturbed atmospheric and electrical conditions.

3.7.5.1 There shall be no outward component of airflow in any part of the work access opening. Smoke released at any point in the plane of the work opening shall pass directly into the front perforated opening.

3.7.5.2 Individual readings of down flow air velocities shall not vary more than ±20 percent or ±16 FPM, whichever is greater, from the calculated zone or row average under clean filter conditions. For exhaust readings by direct measurement at the work zone using a mass flow meter, the variance shall be no more than 5 percent on at least 5 readings.

3.7.5.3 Any individual velocity reading should not vary more than ±20 percent during a 5 second time interval for that particular reading.



3.7.6 Aerosols released anywhere in the work space behind the front perforated opening shall not escape through the work access opening.

3.7.7 Secondary Face Velocity Test: Allowed: if direct measurement technique using a flow hood and secondary measurement using a discrete velocity traverse at the reduced sash opening of 3 inches produces a uniform difference, the manufacturer shall report the conversion/correction factor “CF” on the factory certification report and on the cabinet data label.

3.8 Temperature Rise

3.8.1 The design of the cabinet shall be such to limit the inherent temperature rise within the workspace to 15 degrees Fahrenheit above the ambient room temperature after four hours of continuous operation as described in Paragraph 4.2.5.

3.8.2 BTU Rejection: The supplier shall provide the BTU/hour heat rejection values for each cabinet covered under this specification taking into account heat exhausted from the safety cabinet itself.

3.9 Noise

3.9.1 The overall noise level 12 inches in front of the work opening of the cabinet and 15 inches above the plane of the work surface at the vertical centerline of the cabinet shall not exceed 67 dbA, at a reference sound pressure value of 20 micro-newtons per meter squared (re: 0.0002 dynes per centimeter squared) when measured with the background not over 57 dbA per Paragraph 4.2.7. In addition, there shall be no objectionable pure tone noise component present during the operation of the cabinet.

3.10 Vibration

3.10.1 The maximum displacement of the work tray of the cabinet shall not exceed 100 micro-inches, R.M.S., in any direction when the cabinet is operating at the nominal set point velocities, tested as per Paragraph 4.2.8.

3.10.2 The maximum field vibration limits for each centrifugal fan shall not exceed the following specified limits:

RPM MILS DEFLECTION

900 1.001200 0.751800 0.50

These limits shall apply to the top and side of the motor shaft/fan wheel connection and the fan scroll.

3.11 Leak Tight Construction

3.11.1 Exterior construction of all plenums including welds, gaskets and plenum penetration shall be leak tight as demonstrated by the soap bubble leak test or the alternative pressure decay test. This shall include welds, gaskets, and all plenum penetrations. Testing requirements are described in Paragraph 4.2.1, NSF-49 Annex A and Annex F.

3.11.2 Utility service outlets, as specified, and requiring penetrations through plenum walls or bulkheads, shall meet leak tightness test per Paragraph 4.2.1.

3.12 Labels

3.12.1 Restricted Substances Label: The cabinet shall have a permanently affixed (cemented) label with at least half inch high letters colored white on a red background. The label shall have the following phrase: "DO NOT USE TOXIC, EXPLOSIVE OR FLAMMABLE SUBSTANCES IN THIS CABINET. THE USE OF SMALL AMOUNTS OF CARCINOGENIC, TOXIC, OR RADIOACTIVE SUBSTANCES MUST BE APPROVED BY THE OFFICE OF BIOLOGICAL SAFETY." This label shall be affixed and centered directly above the viewing window.

3.12.2 Manufacturer Label: The manufacturer shall install a label permanently affixed to the front of the cabinet where it will be clearly visible. The label shall be located approximately five feet above the base of the cabinet and shall contain the following information:

(1) The supplier (manufacturer's) name and address

(2) Cabinet type: Class II, Type B2-Hard Connection

(3) The cabinet model number



(4) The cabinet serial number

(5) NSF-49 certified down flow rate in LFPM, and test grid dimensions. For zone flow see paragraph 3.12.2.1

(6) NSF-49 certified face velocity rate in LFPM; Primary and secondary methods and traverse locations

(7) Exhaust HEPA filter size, rating, and quantity

(8) Supply HEPA filter size, rating, and quantity

(9) Factory Certification/Manufacture Date

3.12.2.1 For cabinets designed with multiple velocity distributions in the work zone, the down flow rate shall appear as follows: (5) Certified supply filter airflow rate, LFPM.

3.12.3 Decontamination Signage: The cabinet shall have a permanently affixed white letters on red background label with the following phrase: "THIS CABINET MUST BE DECONTAMINATED PRIOR TO ENTERING OR SERVICING FILTER AREAS, THE FAN/MOTOR EQUIPMENT OR BEFORE MOVING." The label shall be located on the front of the cabinet using at least HALF-inch block lettering.

3.12.4 Field Certification Plate and Recertification Interval: The cabinet shall have a permanently affixed stainless steel, Class 304, No. 4 satin finish plate, minimum U.S.S. Gauge No. 26, 4 inches vertical by 5 inches horizontal. The blank label shall be located 50-60 inches above floor elevation. This plate shall be used to affix subsequent field certification labels.

3.12.5 NSF-49 Seal: The cabinet shall display a current valid NSF-49 seal of certification to be located on a frontal surface.

3.12.6 The manufacturer may include a second manufacturer's label, to be attached at a desired location, provided that this label is not affixed on areas that must be decontaminated routinely or that must be used to attach seals for gas decontamination. If a single label is used and must be located in an area routinely decontaminated such that the label may be covered with duct tape, the label shall be a permanent construction that shall not delaminate.

3.12.7 Initial Field Certification (IFC) Label

3.12.7.1 The cabinet shall have a manufacturer's IFC label affixed to the front horizontal and vertical center of the work zone sash, external side. The label shall be printed in at least 0.25 inch block letters and be removable after field certification.

3.12.7.2 The IFC label should have a white background with red letters and a red border frame.

3.12.7.3 The IFC label shall state: "Warning. This biological safety cabinet shall be field certified according to the latest edition of NSF 49, Field Performance Tests for Personnel, Environmental and Product Protection. This certification is a directive of the Institutional Biosafety Committee."

3.12.8 Ground Fault Circuit Interrupter (GFCI) Label

3.12.8.1 All electrical outlets provided on or inside the biological safety cabinet shall be labeled with ground fault circuit interrupter (GFCI) stickers.

3.12.9 Sash Location Arrows

3.12.9.1 Laminated sash arrows, with white markings on red background, shall be provided on the left and right side of the sash at operating height position.

3.12.10 Operator Instruction Label

3.12.10.1 The supplier shall affix an abbreviated set of operator instructions to the cabinet detailing instructions on the startup, use and shut down of the cabinet.

3.12.10.2 The operator instruction sign shall be plastic laminated and located 60 to 70 inches above floor elevation on the front panel of the cabinet.

3.13 Operations Manual and Service Video



3.13.1 One copy of the operations manual containing the following information shall be furnished with each biological safety cabinet. If an electronic version of this information is to be provided, instructions as to its location and how to access it shall be provided.

(a) Unpacking procedure, purpose, location within the laboratory, cabinet function and description including the proper use of the optional UV lamp

(b) Instructions for installing, operating and performing preventive maintenance on the cabinet

(c) Trouble shooting procedures

(d) List of service parts (identified by supplier's part number) and quantity required for preventive maintenance purposes

(e) HEPA/ULPA Filter - definition, chemical effects, filter life, and procedure for filter replacement

(f) Decontamination procedure

(g) An up to date wiring diagram of all electrical components shall be provided in the operations manual

(h) The fan/motor and motor performance curves should be provided with each cabinet as an attachment to the operations manual.

(i) A hard copy of the test results for all of the Production Quality Control Tests listed in Section 4.2 shall be provided with each cabinet. A copy of the test report shall also be included in the operations manual. The hard copy provided with the cabinet shall indicate that it is for UW-EH&S use only.

3.13.2 One copy of the manufacturer's operations manual for the prototype biological safety cabinet shall be submitted to the University Biological Safety Officer or University Agent.

3.13.3 Service Video: The manufacturer shall have available on request a video presentation of bio safety cabinet maintenance and operation.

3.14 Pressure Gauges, Blower Interlock, and Cabinet Operator Warning Systems, see 3.5.9

3.14.1 Each Class II Type B2-hard connected cabinet shall be equipped with two flush mounted, diaphragm operated, mechanical differential gauge rated for ± five percent full scale accuracy. The gauges shall monitor negative or positive plenum pressure relative to the atmospheric pressure surrounding the cabinet. The filter gauges shall be located in the service control center metal panel, one gauge labeled EXHAUST and one gauge labeled SUPPLY. The gauge scale shall read at least 200 percent of the initial plenum pressure.

3.14.2 The gauge shall be prominently displayed in the front of the cabinet.

3.14.3 The gauge assembly shall conform to paragraph 3.11.

3.14.4 The gauge shall have a front adjustable screw to adjust the needle to zero without disassembly of the gauge or removal of any biological safety cabinet assemblies to access this adjustment screw.

3.14.5 Sash Alarm: A failsafe mechanism shall be provide to alarm when the cabinet window is raised above or lowered below the safe operating height (8 inch) height. The alarm shall shut off when the sash is at the lowest point of travel (closed). The noise level of the alarm shall not exceed 80db-A when a background sound level of less than 55 db-A. The sash alarm switch shall be low voltage 24V or less to reduce the risk of electric shock hazard to certifiers and cabinet operators.

3.14.6 Airflow Monitor and Cabinet Blower Interlock: Each Class II Type B2-hard connected cabinet shall be provided with an automatic air flow monitoring system to alert cabinet operators by set point of a ventilation reduction in the owner supplied exhaust ventilation system. The airflow system shall have both audio and visual alarm mechanisms and be built into the service control center. The airflow monitor shall also serve as a blower interlock to turn off the cabinet internal blower in the event of a 20% or more reduction in airflow in the house exhaust system. Under normal conditions, the airflow monitor shall have a green lamp for normal; if airflow is deteriorating a yellow warning lamp; and red for low or no airflow condition. In red, the airflow monitor shall be programmed to activate an audio alarm. Audio shall should at or below 80 dbA and turn off the cabinet blower. The operator shall be able to turn off the audio alarm, but the visual alarm shall remain on. The interlock circuit shall be designed to prevent the safety cabinet blower from operating until the building exhaust air has been restored.

3.14.7 The airflow monitor shall activate when the cabinet blower switch has been turned on and the airflow monitor will turn off when the operator turns off the blower.



3.14.7.1 The airflow monitor shall be designed to be field adjustable by the cabinet certifier.

3.15 Parts List and Required Parts Discount

3.15.1 The supplier shall provide a parts list by part name and part number for the contract year. The parts list shall be provided in the Operator’s Manual.

3.15.2 Parts price lists should be updated every year prior to the new contract year (renewal years included). The parts list shall be provided to the University Purchasing Department and the University Agent in electronic format.

3.15.3 The supplier shall have available, parts for each cabinet sold under the contract for at least ten years from the last production date of the cabinet model. This shall include but not be limited to motors, ballasts, PC boards, wiring, etc. Replaceable parts such as filters should be available for 20 years from the date of BSC manufacture.

3.15.4 Replacement Parts and Labor: The supplier shall agree to provide warrantee parts for no cost and no shipping charge, FOB UW Environment, Health & Safety Loading Dock for six years starting from the date of delivery at the job site. UW-EH&S Engineering Technical Services, Biological Safety Cabinet Program and the University Agent shall have final authority to make the decision regarding warrantee adjustment and the supplier shall agree to this condition. In the event that any installer on the job, in the sole opinion of the University Agent, has damaged the safety cabinet, the installer shall be liable for replacement parts, shipment and the labor to install said parts and if necessary re-certification or replacement of any biological safety cabinet. Regardless of which person or contractor has actually caused the problem, the General Contractor shall have the final responsibility for payment of damages in the event a sub-contractor or any job employee cannot be proven to be the source of the damages.

3.15.5 Parts Discount: Required: The manufacturer or supplier shall agree to sell non-warranted parts under this contract for 25 percent off the retail price. Non-warranted parts will be warranted for (3) three years.

3.16 Options and Requirements

3.16.1 Required-UW: Bypass armrest with pad – Paragraph 3.2.11.1.1

3.16.2 Option: Adjustable Foot Rest – Paragraph 3.2.11.1.2

3.16.3 Option: Direct Delivery to the laboratory – Paragraph 6.3.1.2

3.16.4 Option: Adaptor rails for bench mounting – Paragraph 3.2.11.2.3.3

3.16.5 Option: Second cable sidewall port -left sidewall – Paragraph 3.2.11.2.1.1.1

3.16.6 Not an option: Automatic assisted lift

3.16.7 Option: UV lamp option-rear wall – Paragraph 3.6.4

3.16.8 Not an option: Animal: Wheels, bumpers and push handle

3.16.9 Option: Additional Petcocks – Paragraph 3.5.8.3.1

3.16.10 Option: Microscope adaptor-Special Request requiring a site visit by the manufacturer – Paragraph 3.4.4.5

3.16.11 Option: Remote valves for each petcock – Paragraph 3.5.8.3.7

3.16.12 Required: Airflow Monitor – Paragraph 3.14.6

3.16.13 Required: Leak Tight Damper – Paragraph 3.2.9.1, 3.2.15, 3.5.2.6, 3.5.2.7, 3.5.10

3.16.14 Option: Reinforced work surface – Paragraph 3.5.2.4.4

3.16.15 Option: All stainless construction – Paragraph 3.2.11.1

3.17 Drawings

3.17.1 Upon request, the supplier shall provide drawings of this workstation detailing the entire front face of this workstation.



3.17.2 Upon request the supplier shall provide a plan view of this work station

3.17.3 PDF files of same maybe sent as fulfilling this requirement; however the supplier shall provide “CAD” drawings if formally requested by the university agent.

3.17.4 Drawings shall be provided in CD or DVD diskettes and will be used in the evaluation of the product or for the design and location of this product within the University Laboratory.

4.0 QUALITY ASSURANCE PROVISIONS

4.1 Qualifications

4.1.1 Procedure for Obtaining Qualification Applicants submitting bids shall submit to the University of Wisconsin a statement on the applicant's willingness to furnish a Class II, Type B2-Hard Connection safety cabinet in conformance with this specification. The statement must also indicate that the equipment under bid conforms to all NSF 49 standards and will display a current NSF 49 seal. As proof of compliance with the above statements the applicant must agree to submit test results, if called upon, for a prototype model tested by NSF. If NSF has made corrections and/or modifications since the prototype tests these changes shall be listed and detailed along with the test results.

4.1.1.1 UL and NSF Seals In the event that an individual purchase calls for an option that invalidates the UL or NSF Seal, the supplier shall note this in the drawing submittal in half inch block letters directly on the drawing, black on white: This design if approved shall not be listed under UL and/or NSF seal because of user requirements.

4.1.2 Prototype Tests: The supplier shall conduct test procedures at the manufacturing facility for prototype models as outlined in the most current edition of NSF-49, Section 6-Performance and Annex A. If requested by the Purchasing Department, the test results shall be provided in written form for review by the University Agent. NSF testing results may also be submitted BUT FOR AN IDENTICAL MODEL PROVIDED UNDER THIS SPECIFICATION. ALTERNATE MODELS MAY BE REJECTED AND THE SUPPLIER GIVEN SIXTY DAYS TO EXECUTE THIS REQUIREMENT AND SUBMIT A COMPLETE TESTING REPORT TO THE PURCHASING DEPARTMENT.

4.2 Production Quality Control Tests Each production biological safety cabinet unit shall meet the following required tests performed by the supplier on the cabinet at the factory. One signed factory test report, hard copy, shall accompany the operations manual for that cabinet. Additional tests may be specified under specific specifications for a particular cabinet.

4.2.1 Pressure Decay/Soap Bubble Test NSF-49 ANNEX A

4.2.2 HEPA Filter Leak Test NSF-49 ANNEX A

4.2.3 Downflow Velocity and Volume NSF-49 ANNEX A

4.2.4 Inflow Velocity and Volume NSF-49 ANNEX A

4.2.4.1 Inflow Velocity determination shall be by NSF-49 Annex A, Test Method A.9.3.2 and A.9.3.4.2.

4.2.5 Temperature Rise Optional NSF-49-1992 ANNEX A

4.2.6 Lighting Intensity NSF-49 ANNEX A

4.2.7 Noise Level NSF-49 ANNEX A

4.2.8 Vibration NSF-49 ANNEX A

4.2.9 UV Intensity Test MDN-02-2010

4.2.10 Electrical Leakage, Ground Circuit Resistance and Polarity NSF-49-1992 ANNEX A

4.2.11 Airflow Smoke Patterns NSF-49 ANNEX A

5.0 PREPARATION FOR DELIVERY

5.1 Packaging

5.1.1 All indicators, gauges and dials shall be covered with cushioning material conforming to PPP-C-843 and the cushioning material shall be secured in place with the tape conforming to PPP-T-60, Type II, Class 1.



5.1.2 All switch boxes, outlets, connections and drain line openings shall be sealed with the tape specified in Paragraph 5.1.1.

5.1.3 Instruction books, leak tight damper assembly, a hard copy of the test report, and parts lists shall be packaged together and secured to the cabinet in a protected location, such as the work zone.

5.1.4 The work tray, sash, front perforated area, air and/or light diffuser, fluorescent light bulb(s), IV bar(s) and UV lamp(s) shall be secured in position by taping, tying, blocking or bracing to prevent movement during transit.

5.1.5 The transition collar from the exhaust filter plenum shall be covered to protect the exhaust filter from shipping damage.

5.1.6 The sash mechanism shall be secured to prevent window, cable, and pulley breakage in shipment.

5.2 Packing

5.2.1 Each Class II biological safety cabinet shall be packed in a container conforming to PPP-B-621, Class 2, style optional; or to PPP-B-601, overseas type; or PPP-B-640, Class 2, Grade A, Style optional. It shall be packed in a crate conforming to MIL-C-3744; nailed assembly, skid type base. The contents of the crate shall be blocked, braced and anchored in accordance with MIL-C-104, and optionally waterproofed with a shroud extending to the base of the crate in accordance with the appendix to MIL-C-132. Strapping shall be in accordance with the appendix to the applicable container specification. Approved Alternative: The cabinet shall be alternatively packed on a wooden skid rated for the shipping load, protected with cardboard walls and top with reinforcement members to face protect the front of the cabinet. The manufacturer shall be responsible for taping all moveable, unsecured cabinet components to ensure against movement during transport.

5.2.2 The shipping container shall be marked on all four sides with warning labels not to drop the contents.

5.2.3 The entire shipping container shall be wrapped in 6 mil construction grade plastic sheeting and or hardboard to protect against rain damage.

5.2.4 On Class II cabinets with a moveable window, the sliding assembly will be braced with wood and taped. No movement shall occur on transit.

5.2.5 Building Address and Room Number Requirement: The cabinet shall be tagged with the building address and room number of the campus laboratory that the unit is going to be installed.

6.0 NOTES

6.1 Guarantee The supplier guarantees the Class II safety cabinet against defective material, workmanship and performance for six years, said guarantee to run from the date of delivery. The supplier agrees to furnish, without cost to the University, replacement of all parts, which are found to be defective during the guarantee period. Cost of installation (labor and decontamination if necessary) of replacement parts and material shall be borne by the supplier, exclusive of transportation charges on returned parts.

6.1.1 Limited Warrantee The supplier warranties the Class II safety cabinet against defective parts for six years from the date of delivery.

6.1.2 Filters and lamp bulbs are perishable and may not be covered under 6.1.1 in the second through sixth years. The University Agent in conjunction with certification personnel will investigate filter and lamp parts to determine responsibility for replacement. The University will be the sole judge on requiring the guarantee on defective filters and lamp bulbs in the three-year period; the supplier shall be consulted.

6.1.3 Contractors: Cabinet filters pre-loaded with construction debris in excess of 25% of the reserve cabinet blower capacity may be replaced by order of the University Agent who has the final authority in determining whether construction debris has been allowed to enter the cabinet work zone and contaminate the filters. The general contractor shall be responsible for any labor or parts replacement costs incurred in the replacement of the cabinet HEPA filters.

6.1.4 Contractors: Cabinet filters damaged with construction debris or labor, may be replaced by order of the

University Agent who has the final authority in determining whether construction activities have resulted in damage to the cabinet. The general contractor shall be responsible for any labor or parts replacement costs incurred.

6.1.5 Replacement Parts Requirement: The manufacturer agrees to provide all parts at a 25% discount rate from retail cost. This shall include the certifier discount.



6.2 Qualification With respect to products requiring NSF 49 certification, awards will be made only for such products as have, prior to the bid opening date, been tested and approved for inclusion in the NSF 49 Products List, whether or not such products have actually been so listed by that date. The attention of suppliers is called to this requirement, and suppliers are urged to arrange to have products that they propose to offer to the University tested for qualification in order that they may be eligible to be awarded contracts or orders for the products covered by this specification. Information pertaining to qualification of products covered by this specification may be obtained from the University Agent, UW-Environment, Health & Safety Department, University of Wisconsin-Madison, 30 East Campus Mall, Madison, Wisconsin 53715.

6.3 Inspection and Certification of Cabinet Delivered FOB Site

6.3.1 Notification of Delivery Supplier shall notify the University of Wisconsin-Madison – Office of Biological Safety at 608-263-2037 or Engineering Technical Services, 30 East Campus Mall, Madison, Wisconsin 53715, telephone: (608)-262-1809, 10 days prior to date of expected delivery at designated FOB site Madison, Wisconsin.

6.3.1.1 Building Address and Room Number: Required: Attached to the safety cabinet underneath the packing plastic or written on cardboard shall be a paper label attached with a string. The label shall have the room number and building or address that the cabinet is being delivered to in order to assist university loading dock personnel, certifiers, and moving and transfer personnel in the final resting location of the safety cabinet. Current program needs for data management require that the location of the biological safety cabinet be identified by the manufacturer PRIOR to shipment.

6.3.1.2 Direct Delivery Option to the Laboratory: Alternative shipment: FOB Room: The supplier upon request shall arrange delivery of the safety cabinet to the lab space of the designated room at the University. The supplier shall therefore list as an option direct delivery cost to the laboratory and assume all delivery and moving liability.

6.3.2 Dock Inspection User/Purchaser will notify the UW Environment, Health & Safety – Engineering Technical Services group of the cabinet's arrival. Gross inspection will be made for punctures, breakage or other signs of damage to packaging material and/or cabinet. Notation will be made of defects observed. Supplier will be promptly notified of any defects or deficiencies in the cabinet. Following disassembly of outer packing and further inspection for damages, completeness of cabinet and options, authorization (by the UW-Environment, Health & Safety – Engineering Technical Services) will be given to the user to arrange for transfer to the designated laboratory. The user and the Engineering Technical Services/Office of Biological Safety will have previously arranged the site, utility proximity and other installation considerations for the cabinet.

† NOTE TO CUSTOMERS AND RECEIVING PERSONNEL: ALL UNIT SHIPMENTS ARE MONITORED DURING TRANSIT. UNIT SHIPMENTS CONTAIN A DROP (N) TELL IMPACT INDICATOR TO IDENTIFY IN TRANSIT SHIPMENT DAMAGE AND CONCEALED IN TRANSIT SHIPMENT DAMAGE. ALL SHIPPING CONTAINERS WILL BE CLEARLY MARKED.

6.3.2.1 Hidden Damage The University shall not be liable for hidden damage. It will be the responsibility of the supplier, shipper and/or moving and transfer agent to correct any hidden/undisclosed damages. The University shall notify the interested party(s) of any damage that is discovered. The cabinet supplier shall have the primary liability for resolving hidden damages with all parties concerned including replacing parts, the costs for labor or the cabinet itself.

6.3.2.2 Replacement Parts or Cabinet The cabinet is a custom made unit manufactured to controlled University specifications. Therefore if standard replacement parts are called for, the supplier shall have 14 days to ship the parts to the University. If custom made parts or the entire cabinet is damaged, the supplier has 60 days to replace these parts or cabinet.

6.3.2.3 Extreme Needs Situation on Damaged Goods In an extreme needs situation, the University reserves the option to replace the damaged goods with non-specification grade parts or entire cabinet assembly. In this extreme situation Standard supplier terms as listed in the supplier’s catalog will be honored. UW Purchasing will contact the supplier and authorize to proceed.

6.3.3 Certification Tests Upon installation, cabinet certification tests (paid by user) will be performed on the installed

cabinet by UW-Environment, Health & Safety – Engineering Technical Services. These tests will follow those outlined in NSF-49 Annex F, both in scope and method of performance. In addition, a filter load test to determine fulfillment of the "10/100" requirement as stipulated in Paragraph 3.5.6.3 may be made by the Engineering Technical Services group on selected cabinets if there is some question of the fan/motor performance graphs cited in Paragraph 3.1.1. Cabinet site tests will be compared to results of factory control tests, which accompany the operator's manual for the cabinet supplied.



6.3.3.1 The required initial certification has been mandated by the University Institutional Biosafety Committee for all biological safety cabinets, whether purchased for non-hazardous or bio-hazardous projects. The certification shall be performed by the UW-Environment, Health & Safety – Engineering Technical Services group under the direction of the University Agent or other personnel who are NSF 49 Accredited for field testing of biological safety cabinets. Each safety cabinet will be tested and certified.

6.3.3.2 Contact the UW-Environment, Health & Safety – Engineering Technical Services group at 608-262-1809 or [email protected] for certification and inspection service.

6.3.4 Penalties for Noncompliance Labor for minor corrections needed to achieve certification beyond one hour normally allotted will be charged to the supplier at the rate of $200.00 per hour. Failure of the cabinet to meet any of the tests specified (unless only minor correction suffices) or structural material requirements shall constitute grounds for rejection. The cabinet will be returned at the supplier's expense unless remedial action (including parts and labor) is provided within 30 days. This corrective action must be appropriate and satisfactory in the opinion of the Engineering Technical Services group and purchaser and enable the cabinet to pass the specified certification tests. At the option of the supplier corrective labor necessary to satisfy the performance can be performed by the Engineering Technical Services group at the rate stipulated above ($200.00/hour).

6.4 Fabrication and Delivery Time Once drawing approval is given on a cabinet, the supplier shall commence fabrication within thirty days. Shipment should be made within sixty days of drawing approval. The supplier shall complete an individual cabinet order no later than two months from the time the drawing approval is issued. TWO months from the time of approval to shipment from the supplier’s loading dock is considered the maximum time and shall not occur more than three times per twelve month period. The supplier shall contact the University Agent in order to clarify any conflicts in shipping and specification compliance to ensure acceptable delivery time.

6.4.1 Inspection and Certification of Cabinet Delivered FOB Laboratory: The University requests that the supplier prepare an optional cost for delivery FOB laboratory room. Shipping Inspection will be during certification in the final “AS IS” location.


mailto:[email protected]


APPENDIX AGRAPH 1

Example Motor Performance Graph (Specification 3.1.1.1)

MOTOR MANUFACTURER:MOTOR MODEL NUMBER:MOTOR HORSEPOWER: (HP)CAPACITOR SIZE: (MFD)

T= MOTOR TORQUE OUNCE FEETEC= VOLTAGE ACROSS CAPACITOR

T - OUNCE FEET x MULT. I= MOTOR AMPERESEC - x MULT. RPM= REVOLUTIONS PER MINUTEI - x MULT. MFD= MICROFARAD



APPENDIX AGRAPH 2

Example Fan Motor Performance Graph (Specification 3.1.1.2)

3.1.1.2.4.4 Clean filters with cabinet air velocities required under Paragraph 3.7.QT = CFM, Motor Speed = RPM, Filter Pressure Drop = IWC

3.1.1.2.4.5 Loaded filters to produce a 10 percent reduction in total airflow.QT = CFM, Motor Speed = RPM, Filter Pressure Drop = IWC

FAN MANUFACTURER: FAN MODEL NUMBER: MOTOR MANUFACTURER: MOTOR MODEL NUMBER:

OPERATIONAL TORQUE DEFINED BYTHE EXPRESSION:

T2 = T1/(EC1/EC2)e2T2 = OPERATIONAL TORQUE - OUNCE FEETT1 = TORQUE AT 115 VOLTS AC-OUNCE FEETEC1 = 115 VOLTS ACEC2 = OPERATIONAL VOLTAGE

QT=FAN AIRFLOW-CFM-STANDARD AIRSP=FAN STATIC PRESSURE-IWCIWC=INCHES WATER COLUMN

Required minimum load factor is 100% as reported in graph 3.5.6.3



APPENDIX BFIGURE 1

Bench Model - Class II, Type B2-Hard Connection (Taken from Paragraph 3.3)

NOT TO SCALE

DIMENSIONAL REQUIREMENTS IN INCHES

A(RANGE)

B(RANGE)

C(RANGE)

D(RANGE)

E(RANGE)

F(RANGE)

G(RANGE)

H(RANGE)

4 ft Bench 54(53-55)

34(33-35)

62(61-63)

46(45-47)

25(24-26)

28(27-29)

92(88 -97)

28(27-35)

6 ft Bench 78(77-79)

34(33-35)

62(61-63)

70(69-71)

25(24-26)

28(27-29)

92(88 -97)

28(27-35)


UNIVERSITY OF WISCONSIN- · Web viewUNIVERSITY OF WISCONSIN-MADISON SPECIFICATION UW-MDN-06-B2-2015 GENERAL PURPOSE CLEAN AIR BIOLOGICAL SAFETY CABINET CLASS II, TYPE B2 – 100%...

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