University Faculty Details Page on ... - Delhi University

www.du.ac.in Page 1

Title Prof./Dr./Mr./

Ms. Prof.

First Name Vijay K. Last Name

Chaudhary Photograph

Designation Professor

Department Biochemistry

Address (Campus)

University of Delhi South campus, New Delhi 110021

(Residence) 1GF, Type 5A Flats University of Delhi South campus, New Delhi 110021

Phone No (Campus)

91-11-24111955, ext 156

(Residence)optional 91-11-24115863

Mobile 91-9811800434

Fax 91-11-24115270

Email [email protected],

Web-Page

Education

Subject Institution Year Details

Ph.D Biochemistry University of Delhi 1983

M. Sc Biochemistry G.B.Pant University, Pantnagar, UT

1975

B. Sc Biochemistry Agra University 1973

Career Profile

Organisation / Institution Designation Duration Role

Department of Biochemistry, University of Delhi South campus, New Delhi (DBUDSC)

Professor 1996-date Teaching and Research

DBUDSC, New Delhi Professor and Head 1999-2002, 2011-2014

Teaching and Research

DBUDSC, New Delhi Head 1993-1996 Teaching and Research

DBUDSC, New Delhi Reader 1990-1996 Teaching and Research

Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, USA

Visiting Fellow and Visiting Associate

1985-1990 Research

Escorts Medical Centre, and N.I.T. Faridabad

Clinical Biochemist

1981-1985 Headed Biochemistry Laboratory

Research Interests / Specialization

Research interests- Novel Reagents and Test Devices for Diagnosis of Infectious Diseases (AIDS, Tuberculosis, Malaria and Typhoid), Directed Evolution of Antibodies and Therapeutic Proteins, Development of Phage display Vectors, Phage display based search of small molecule binders for Mycobacterial Proteome. Human antibodies. Specialization- Recombinant DNA technology, Monoclonal antibodies, Clinical Microbiology, Phage Display Technology, Antibody Engineering, High throughput genome-wide cloning, expression and Purification of Proteins, Production of Therapeutic Proteins.

Teaching Experience (Subjects/Courses Taught)

1990-Present - Recombinant DNA Technology, Functional Genomics, Antibody Engineering, DNA Sequencing, Expression systems and strategies for recombinant protein production.

Honors & Awards

University Faculty Details Page on DU Web-site

www.du.ac.in Page 2

Visitor’s Award for Innovation-2015 by the President of India at a special ceremony at Rashtrapati Bhawan on 4 February 2015

2014, Biotech Product and Process Development and Commercialization Award, Department of Biotechnology, Government of India.

Bachhawat Memorial Lecture award (2009) by National Academy of Sciences India.

2005, WIPO (World Intellectual Property Organization, Geneva) Gold Medal for “Best Invention of the Year 2004” for inventing “On-site Detection of HIV (AIDS).

2004, National Research Development Corporation (NRDC, DSIR, Ministry of Science & Technology, Government of India) award of Rs. 1,50,000/- for inventing “On-site Detection of HIV (AIDS)”

2002, Biotech Product and Process Development and Commercialization Award, Department of Biotechnology, Government of India.

1999, All India Biotech Association (AIBA) Award

1997,VASVIK Award for Biological Science and Technology

1991, the National Institutes of Health, USA (NIH) Director’s Award

Fellow of the National Academy of Sciences

Member, Guha Research Conference.

Publications (LAST FIVE YEARS)

Books / Monographs Year of Publication

Title Publisher Co-Author

2005

Phage Display: A molecular fashion show. Manuscript in book entitled "Phages: Their Role in Bacterial Pathogenesis and Biotechnology"

American Society of Microbiology (ASM)

Oppenheim, A Gupta.

In Indexed/ Peer Reviewed Journals Year of Publication

Title Journal Co-Author

2003 Whole-Blood Agglutination Assay For On-Site detection of Human Immunodeficiency Virus infection.

J.Clin. Micrbiol. Vol 41(7) Pg 2814-2821

Gupta A.

2003 High Density Functional Display of Proteins on Bacteriophage Lambda

J. Mol. Biol., Vol 334(2) Pg 241-254

Masanori Onda, Ira Pastan, Sankar Adhya

,

and Gupta A.

2005 Expression and purification of recombinant 38-kDa and Mtb81 antigens of Mycobacterium tuberculosis for application in serodiagnosis

Protein Expression Purification

40:169-176

Gupta A., Kulshreshta, A., Gupta, G., Verma, N., Kumari, S., Sharma, S.K., Anil K. Tyagi.

2005 Serogroup-reactive and type-specific detection of bluetongue virus antibodies using chicken scFvs in inhibition ELISAs.

J Virol Methods Vol 129(1) Pg 31-39

Fehrsen J, van Wyngaardt W, Mashau C, Potgieter AC, Gupta A., Jordaan FA, du Plessis DH

2005 Expression and purification of recombinant antigens of Mycobacterium tuberculosis for application in serodiagnosis.

Protein Expression Purification Vol 44 Pg75-85.

Abhishek Kulshreshtha, Nitin Verma, S. K. Sharma, Anil K. Tyagi and Gupta A.

2006 Bifunctional recombinant fusion proteins for rapid detection of antibodies to both HIV-1 and HIV-2 in whole blood.

BMC Biotechnology Vol 6 Pg 39

Gupta A.

2009 Directed evolution of an anti-human MAbs. 1(3):268- Gupta A.and Rajiv Bhat.

www.du.ac.in Page 3

2012 2012 2013 2014 2015 2015

red blood cell antibody. Mapping interactions of Chikungunya virus nonstructural proteins.

Intraviral protein interactions of Chandipura virus. A Novel Helper Phage Enabling Construction Of Genome-Scale ORF-Enriched Phage Display Libraries

Rapid restriction enzyme-free cloning of PCR products: a high-throughput method applicable for library construction

Host interactions of Chandipura virus

matrix protein.

Host-pathogen interactome analysis

of Chikungunya virus envelope

proteins E1 and E2.

80 Virus Res. Oct;169(1):231-6 Arch Virol. 2012 Oct; 157(10): 1949-57 PlosOne (In Press)

PLoS One, 9(10): e111538

Acta Trop.

149:27-31

Virus Genes.

50(2):200-9.

Sreejith R, Rana J, Dudha N, Kumar K, Gabrani R, Sharma SK, Gupta A, Vrati S, Gupta S. Kumar K, Rana J, Sreejith R, Gabrani R, Sharma SK, Gupta A, Gupta Amita Gupta, Nimisha Shrivastava, Payal Grover, Ajay Singh, Kapil Mathur, Vaishali Verma, Charanpreet Kaur V.K. Chaudhary , N. Shrivastava, P. Grover, V. Verma, C. Kaur, and Shilpi Das Rajasekharan S, Kumar K, Rana J, Gupta A, Chaudhary VK, Gupta S. Dudha N, Rana J, Rajasekharan S, Gabrani R, Gupta A, Chaudhary VK, Gupta S.

Articles

Conference Presentations

Total Publication Profile optional

Books Two

In Indexed/ Peer Reviewed Journals Seventy Five publications and Fourteen Indian and International patents

Articles

Conference Presentations Thirty

Public Service / University Service / Consulting Activity

Chairman, Interim Governing Council WUS Health Centre (IGC-WUSHC), WUS Health Centres, University of Delhi.

Member, Expert Committee on Tuberculosis, Department of Biotechnology, Government of India, New Delhi, 2006 to 2014.

Member, Task Force on DBT-Boost to University Interdisciplinary Life Sciences for Education and Research (DBT-BUILDER) Department of Biotechnology, Government of India, New

www.du.ac.in Page 4

Delhi, 2009 onwards.

Member, Committee for Innovative Young Biotechnologists Award, Department of Biotechnology, Government of India, New Delhi, 2005 to 2014.

Co-Chaiman, Task Force for Vaccines and Diagnostics in areas of health care, Department of Biotechnology, Government of India, New Delhi, 2005-2009.

Member, Task Force for Infectious Disease Biology, DBT, New Delhi, 2005-2009.

Member, Project Review Committee for Allergy, Biochemistry and Immunology, ICMR New Delhi. 2014-

Member Expert, Research Council of Indian Institute of Toxicology Research, Lucknow, 2006 -2009onwards.

Member, Board of Research Studies, Faculty of Inter Disciplinary and Applied Sciences, University of Delhi, 2008-2014.

Member, Governing Bogy, Technology-Based Incubator, University of Delhi South Campus, New Delhi, 110021. 2011-

Member of the Academic Committee, Central Drug Research Institute, Lucknow, 2005- 2008.

Member of the Academic Committee, Centre for Cellular and Molecular Biology, Hyderabad, 2008 2011.

Member of SAC, Institute of Pathology, Safdarjung Hospital, New Delhi, 2004-2008.

Member of SAC, NIRT/NJILOMD SAC, Chennai/Agra, 2011-2015.

University Representative on the Governing Body, Sri Aurobindo College, 2007-2009.2012-2013

University Representative on the Governing Body, Motilal Nehru College, New Delhi, 2010-2013.

University Representative on the Governing Body, Desh Bandhu College, New Delhi, 2013-2015.

University Representative on the Governing Body, Ram Lal Anand College, New Delhi, 2008-10, 2013-2015.

University Representative on the Governing Body, Aryabhatta college, New Delhi, 2014-2016

Chairman Building Committee, , Ram Lal Anand College & Aryabhatta college, 2013-2015/6

Member, Academic Council, University of Delhi, 1993-1996, 1999-2002, 2011-2014 (April 2015)

Member, University Court, University of Delhi, 1996 onwards.

Member Scientific Advisory Committee, M/s Yashraj Biotech, Navi Mumbai 2010-

Advisor, Cadila Pharmaceuticals Limited, Ahmedabad, 2007-2011.

Advisor, Century Pharmaceuticals Limited, Baroda, 2007-08.

Advisor, Ranbaxy Laboratories Limited, Delhi, 1991-1995, 2000-2003.

Member, Scientific Advisory Committee, SPAN Diagnostics, Surat, 2006- 2014

Professional Societies Memberships

Life member of Society of Biological Chemists (India). Life member of Association of Clinical Biochemists of India. Life member of Society of Biological Chemists (India). Life member of Indian Science Congress Association Life member of Association of Microbiologists of India.

Projects (Major Grants / Collaborations)

NMITLI Project "Development and production of a therapeutic monoclonal antibody against

eNAMPT, a novel inflammatory target" The total project cost is Rs.2299.13 lakh for project duration

of five years (March 2011-2016) with loan assistance of Rs. 2121.64 lakh to the industrial partner,

Gennova Biopharmaceuticals Limited (GBL), Pune and Rs.275 lacs to UDSC as grant-in-aid.

"Development of reagents for simple immunochemical tests for the detection of Chikungunya infection". DBT. Duration: 2014- 17; Amount: Rs.89 lacs

DNA sequencing facility at UDSC (Phase V) : DBT. Duration: 2014- 17; Amount: Rs.117.588 lacs

Other Details

Currently, the main focus of the laboratory is to identify Biomarkers, and develop reagents, which can be used for developing rapid but sensitive detection systems for the diagnosis of infectious diseases including HIV-AIDS, Tuberculosis, Chikungunya, Dengue, Typhoid and Malaria. For sensitive detection of antigens, monoclonal antibodies are produced by conventional hybridoma technology. The high affinity clones and non-completing pairs of MAbs are identified by pair-wise epitope mapping using surface plasmon Resonance (SPR, BIACore 3000). The affinity and folding characteristics of MAbs are improved by Phage display based mutagenesis and selection and recombinant antibody fragments are produced. For antibody detection, high quality proteins are

www.du.ac.in Page 5

produced following high throughput strategies for cloning, expression and purification. These reagents (high affinity MAbs and purified Proteins) are employed for developing point-of-care tests using various rapid assay formats with colloidal-Gold and fluorescence labeled Nano-particles. The group has interactions with Academic and Industrial R&D Centres involved in Diagnostics Research in India, and Finland. Some of the recently proposed/initiated programmes include:

Engineering of Insoluble/partially soluble antigens to produce soluble immunoreactive variants.

Directed Evolution of antibodies for antigen detection in tuberculosis.

Genome-wide search for new antigens to detect antibodies in serum and other fluids of tuberculosis patients.

Highly sensitive TRF based detection of antigen and antibodies.

On-the-spot detection of M. tuberculosis bacteria in sputum using fluorescently tagged antibodies.

Human antibody Libraries

Selected Awarded Patents:

Improved process for expression, purification and enhanced recovery of mycobacterial

recombinant proteins. Tyagi, Anil K., Chaudhary, Vijay K., Gupta, Amita and Kushrestha, Abhishek

(Indian Patent No IN263766), awarded 25th October 2014.

Lambda phage display system and The Process, Vijay Kumar Chaudhary, Amita Gupta, Sankar

Adhya, Ira H. Pastan, US 7,410,801, August12, 2008.

A process for the isolation and purification of protein p17 of HIV-1 subtype C. (No. 808/Del/2003)

A process for the isolation and purification of protein p24 of HIV-1 subtype C. (No. 1478/Del/99)

Target-specific, Cytotoxic, Recombinant Pseudomonas Exotoxin, Ira Pastan, Vijay K. Chaudhary,

David Fitzgerald, US Patent No.5, 705,163, January 6, 1998.

Monoclonal Antibodies (MAbs) against two coat proteins gIIIp and gVIIIp of filamentous phage M13

and a process for their preparation. Patent No.764/Del/94, dated 20 October 1997.

Technology Transferred and commercialized

The technology of production of “TBConfirm” test was transferred to M/s SPAN Diagnostics

Limited, Surat on 26th August 2011. The Drug Controller General of India has approved the

test for commercial production. The test should be available in the market very soon.

A rapid test to detect antibodies to HIV in a drop of blood. This technology was transferred to a

M/s Cadila Pharmaceuticals Limited Ahmedabad, that manufactures and markets it under the trade

name “NEVA-HIV” (Naked eye visible agglutination assay for HIV).

Monoclonal antibodies against major and minor coat proteins of bacteriophage M13 and were

licensed to M/s Pharmacia (now GE Health Care) and is available as Cat No. 27-9420-01

Publications of Professor Vijay K Chaudhary, Department of Biochemistry, Delhi University

1. Rajasekharan S, Kumar K, Rana J, Gupta A, Chaudhary VK, Gupta S. Host interactions of

Chandipura virus matrix protein. Acta Trop. 2015 Sep; 149:27-31. doi: 10.1016/j.actatropica.2015.04.027. Epub 2015 May 2.

2. Singh V, Kaur C, Chaudhary VK, Rao KV, Chatterjee S. M. tuberculosis Secretory Protein

ESAT-6 Induces Metabolic Flux Perturbations to Drive Foamy Macrophage Differentiation. Sci Rep. 2015 Aug 7;5:12906. doi: 10.1038/srep12906.

3. Dudha N, Rana J, Rajasekharan S, Gabrani R, Gupta A, Chaudhary VK, Gupta S. Host-

pathogen interactome analysis of Chikungunya virus envelope proteins E1 and E2. Virus Genes. 2015 Apr;50(2):200-9. doi: 10.1007/s11262-014-1161-x. Epub 2015 Jan 7.

4. Singh S, Dey B, Sachdeva KS, Kabra SK, Chopra KK, Chaudhary VK, Sharma P, Katoch

VM. Challenges in tuberculosis diagnosis and management: recommendations of the expert panel. J Lab Physicians. 2015 Jan-Jun;7(1):1-3. doi: 10.4103/0974-2727.154778.

5. Rana J, Rajasekharan S, Gulati S, Dudha N, Gupta A, Chaudhary VK, Gupta S. Network

mapping among the functional domains of Chikungunya virus nonstructural proteins. Proteins. 2014 Oct;82(10):2403-11. doi: 10.1002/prot.24602. Epub 2014 May 30.

6. Chaudhary VK, Shrivastava N, Verma V, Das S, Kaur C, Grover P, Gupta A. Rapid

restriction enzyme-free cloning of PCR products: a high-throughput method applicable for library construction. PLoS One. 2014 Oct 31;9(10):e111538. doi: 10.1371/journal.pone.0111538. eCollection 2014.

7. Dobhal S, Chaudhary VK, Singh A, Pandey D, Kumar A, Agrawal S. Expression of

recombinant antibody (single chain antibody fragment) in transgenic plant Nicotiana tabacum cv. Xanthi. Mol Biol Rep. 2013 Dec;40(12):7027-37. doi: 10.1007/s11033-013-2822-x. Epub 2013 Nov 12.

8. Kumar K, Rajasekharan S, Gulati S, Rana J, Gabrani R, Jain CK, Gupta A, Chaudhary VK,

Gupta S. Elucidating the interacting domains of chandipura virus nucleocapsid protein. Adv Virol. 2013;2013:594319. doi: 10.1155/2013/594319. Epub 2013 Oct 28.

9. Gupta A, Shrivastava N, Grover P, Singh A, Mathur K, Verma V, Kaur C, Chaudhary VK. A

novel helper phage enabling construction of genome-scale ORF-enriched phage display libraries. PLoS One. 2013 Sep 27;8(9):e75212. doi: 10.1371/journal.pone.0075212. eCollection 2013.

10. Kumar K, Rana J, Sreejith R, Gabrani R, Sharma SK, Gupta A, Chaudhary VK, Gupta S.

Intraviral protein interactions of Chandipura virus. Arch Virol. 2012 Oct;157(10):1949-57. Epub 2012 Jul 5.

11. Sreejith R, Rana J, Dudha N, Kumar K, Gabrani R, Sharma SK, Gupta A, Vrati S, Chaudhary

VK, Gupta S. Mapping interactions of Chikungunya virus nonstructural proteins. Virus Res. 2012 Oct;169(1):231-6. doi: 10.1016/j.virusres.2012.08.006. Epub 2012 Aug 19. PubMed

12. Gupta A, Chaudhary VK, Bhat R. Directed evolution of an anti-human red blood cell

antibody. MAbs. 2009 May-Jun;1(3):268-80.

13. Gupta A, Chaudhary VK. Bifunctional recombinant fusion proteins for rapid detection of antibodies to both HIV-1 and HIV-2 in whole blood. BMC Biotechnol. 2006 Sep 22; 6:39.

14. Kulshrestha A, Gupta A, Verma N, Sharma SK, Tyagi AK, Chaudhary VK. Expression and

purification of recombinant antigens of Mycobacterium tuberculosis for application in serodiagnosis. Protein Expr Purif. 2005 Nov;44(1):75-85.

15. Fehrsen J, van Wyngaardt W, Mashau C, Potgieter AC, Chaudhary VK, Gupta A, Jordaan FA, du Plessis DH. Serogroup-reactive and type-specific detection of bluetongue virus antibodies using chicken scFvs in inhibition ELISAs. J Virol Methods. 2005 Oct; 129(1):31-9.

16. Chaudhary VK, Kulshreshta A, Gupta G, Verma N, Kumari S, Sharma SK, Gupta A, Tyagi

AK. Expression and purification of recombinant 38-kDa and Mtb81 antigens of Mycobacterium tuberculosis for application in serodiagnosis. Protein Expr Purif. 2005 Mar;40(1):169-76.

17. Gupta A, Onda M, Pastan I, Adhya S, Chaudhary VK. High-density functional display of

proteins on bacteriophage lambda. J Mol Biol. 2003 Nov 21;334(2):241-54. PubMed PMID: 14607116.

18. Gupta A, Chaudhary VK. Whole-blood agglutination assay for on-site detection of human

immunodeficiency virus infection. J Clin Microbiol. 2003 Jul;41(7):2814-21.

19. Gupta A, Chaudhary VK. Expression, purification, and characterization of an anti-RBCFab-p24 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood. Protein Expr Purif. 2002 Oct; 26(1):162-70.

20. Gupta R, Singh BP, Sridhara S, Gaur SN, Kumar R, Chaudhary VK, Arora N. Allergenic

cross-reactivity of Curvularia lunata with other airborne fungal species. Allergy. 2002 Jul; 57(7):636-40.

21. Gupta R, Singh BP, Sridhara S, Gaur SN, Kumar R, Chaudhary VK, Arora N. Identification of

cross-reactive proteins amongst different Curvularia species. Int Arch Allergy Immunol. 2002 Jan;127(1):38-46.

22. Gupta A, Gupta S, Chaudhary VK. Recombinant fusion proteins for haemagglutination-

based rapid detection of antibodies to HIV in whole blood. J Immunol Methods. 2001 Oct 1; 256(1-2):121-40.

23. Gupta S, Arora K, Sampath A, Singh SS, Gupta A, Chaudhary VK. Mapping of hiv-1 Gag

epitopes recognized by polyclonal antibodies using gene-fragment phage display system. Prep Biochem Biotechnol. 2001 May;31(2):185-200.

24. Gupta S, Arora K, Gupta A, Chaudhary VK. Gag-derived proteins of HIV-1 isolates from

Indian patients: cloning, expression, and purification of p17 of B- and C-subtypes. Protein Expr Purif. 2001 Apr; 21 (3):378-85.

25. Gupta S, Arora K, Gupta A, Chaudhary VK. Gag-derived proteins of HIV-1 isolates from

Indian patients: cloning, expression, and purification of p24 of B- and C-subtypes. Protein Expr Purif. 2000 Aug;19 (3):321-8.

26. Gupta R, Singh BP, Sridhara S, Gaur SN, Chaudhary VK, Arora N. Allergens of Curvularia

lunata during cultivation in different media. J Allergy Clin Immunol. 1999 Oct;104 (4 Pt 1):857-62.

27. Gupta S, Arora K, Sampath A, Khurana S, Singh SS, Gupta A, Chaudhary VK. Simplified

gene-fragment phage display system for epitope mapping. Biotechniques. 1999 Aug;27(2):328-30, 332-4.

28. Sampath A, Abrol S, Chaudhary VK. Versatile vectors for direct cloning and ligation-

independent cloning of PCR-amplified fragments for surface display on filamentous bacteriophages. Gene. 1997 Apr 29;190(1):5-10.

29. Sharma M, Swain PK, Chopra AP, Chaudhary VK, Singh Y. Expression and purification of

anthrax toxin protective antigen from Escherichia coli. Protein Expr Purif. 1996 Feb;7(1):33-8.

30. Bhardwaj D, Singh SS, Abrol S, Chaudhary VK. Monoclonal antibodies against a minor and the major coat proteins of filamentous phage M13: their application in phage display. J Immunol Methods. 1995 Feb 27;179(2):165-75.

31. Kushwaha A, Chowdhury PS, Arora K, Abrol S, Chaudhary VK. Construction and

characterization of M13 bacteriophages displaying functional IgG-binding domains of staphylococcal protein A. Gene. 1994 Dec 30;151(1-2):45-51.

32. Abrol S, Sampath A, Arora K, Chaudhary VK. Construction and characterization of M13

bacteriophages displaying gp120 binding domains of human CD4. Indian J Biochem Biophys. 1994 Aug;31 (4):302-9.

33. Chowdhury PS, Kushwaha A, Abrol S, Chaudhary VK. An expression system for secretion

and purification of a genetically engineered thermostable chimera of protein A and alkaline phosphatase. Protein Expr Purif. 1994 Feb;5(1):89-95.

34. Kreitman RJ, Bailon P, Chaudhary VK, FitzGerald DJ, Pastan I. Recombinant immunotoxins

containing anti-Tac(Fv) and derivatives of Pseudomonas exotoxin produce complete regression in mice of an interleukin-2 receptor-expressing human carcinoma. Blood. 1994 Jan 15;83(2):426-34.

35. Abrol S, Chaudhary VK. Excess of PCR primers inhibit DNA cleavage by some restriction

endonucleases. Biotechniques. 1993 Oct;15(4):630, 632.

36. Kreitman RJ, Chaudhary VK, Waldmann TA, Hanchard B, Cranston B, FitzGerald DJ, Pastan I. Cytotoxic activities of recombinant immunotoxins composed of Pseudomonas toxin or diphtheria toxin toward lymphocytes from patients with adult T-cell leukemia. Leukemia. 1993 Apr;7 (4):553-62.

37. Kreitman RJ, Batra JK, Seetharam S, Chaudhary VK, FitzGerald DJ, Pastan I. Single-chain

immunotoxin fusions between anti-Tac and Pseudomonas exotoxin: relative importance of the two toxin disulfide bonds. Bioconjug Chem. 1993 Mar-Apr;4(2):112-20.

38. Batra JK, Kasturi S, Gallo MG, Voorman RL, Maio SM, Chaudhary VK, Pastan I. Insertion of

constant region domains of human IgG1 into CD4-PE40 increases its plasma half-life. Mol Immunol. 1993 Mar;30 (4):379-86.

39. Kreitman RJ, Chaudhary VK, Kozak RW, FitzGerald DJ, Waldman TA, Pastan I.

Recombinant toxins containing the variable domains of the anti-Tac monoclonal antibody to the interleukin-2 receptor kill malignant cells from patients with chronic lymphocytic leukemia. Blood. 1992 Nov 1;80 (9):2344-52.

40. Pastan I, Chaudhary V, FitzGerald DJ. Recombinant toxins as novel therapeutic agents.

Annu Rev Biochem. 1992;61:331-54. Review.

41. Kreitman RJ, Siegall CB, Chaudhary VK, FitzGerald DJ, Pastan I. Properties of chimeric toxins with two recognition domains: interleukin 6 and transforming growth factor alpha at different locations in Pseudomonas exotoxin. Bioconjug Chem. 1992 Jan-Feb;3(1):63-8.

42. Kreitman RJ, Chaudhary VK, Siegall CB, FitzGerald DJ, Pastan I. Rational design of a

chimeric toxin: an intramolecular location for the insertion of transforming growth factor alpha within Pseudomonas exotoxin as a targeting ligand. Bioconjug Chem. 1992 Jan-Feb;3(1):58-62.

43. FitzGerald D, Chaudhary VK, Kreitman RJ, Siegall CB, Pastan I. Generation of chimeric

toxins. Targeted Diagn Ther. 1992;7:447-62. Review. 44. Chaudhary VK, FitzGerald DJ, Pastan I. A proper amino terminus of diphtheria toxin is

important for cytotoxicity. Biochem Biophys Res Commun. 1991 Oct 31;180 (2):545-51.

45. Seetharam S, Chaudhary VK, FitzGerald D, Pastan I. Increased cytotoxic activity of Pseudomonas exotoxin and two chimeric toxins ending in KDEL. J Biol Chem. 1991 Sep 15;266(26):17376-81.

46. Singh Y, Klimpel KR, Quinn CP, Chaudhary VK, Leppla SH. The carboxyl-terminal end of

protective antigen is required for receptor binding and anthrax toxin activity. J Biol Chem. 1991 Aug 15;266(23):15493-7.

47. Batra JK, Fitzgerald DJ, Chaudhary VK, Pastan I. Single-chain immunotoxins directed at the

human transferrin receptor containing Pseudomonas exotoxin A or diphtheria toxin: anti-TFR(Fv)-PE40 and DT388-anti-TFR(Fv). Mol Cell Biol. 1991 Apr;11 (4):2200-5.

48. Chaudhary VK, Gallo MG, FitzGerald DJ, Pastan I. A recombinant single-chain immunotoxin

composed of anti-Tac variable regions and a truncated diphtheria toxin. Proc Natl Acad Sci U S A. 1990 Dec;87 (23):9491-4.

49. Ogata M, Chaudhary VK, Pastan I, FitzGerald DJ. Processing of Pseudomonas exotoxin by

a cellular protease results in the generation of a 37,000-Da toxin fragment that is translocated to the cytosol. J Biol Chem. 1990 Nov 25;265 (33):20678-85.

50. Kreitman RJ, Chaudhary VK, Waldmann T, Willingham MC, FitzGerald DJ, Pastan I. The

recombinant immunotoxin anti-Tac(Fv)-Pseudomonas exotoxin 40 is cytotoxic toward peripheral blood malignant cells from patients with adult T-cell leukemia. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8291-5.

51. Ashorn P, Moss B, Weinstein JN, Chaudhary VK, FitzGerald DJ, Pastan I, Berger EA.

Elimination of infectious human immunodeficiency virus from human T-cell cultures by synergistic action of CD4-Pseudomonas exotoxin and reverse transcriptase inhibitors. Proc Natl Acad Sci U S A. 1990 Nov;87 (22):8889-93.

52. Chaudhary VK, Jinno Y, Gallo MG, FitzGerald D, Pastan I. Mutagenesis of Pseudomonas

exotoxin in identification of sequences responsible for the animal toxicity. J Biol Chem. 1990 Sep 25;265 (27):16306-10.

53. Lorberboum-Galski H, Garsia RJ, Gately M, Brown PS, Clark RE, Waldmann TA, Chaudhary

VK, FitzGerald DJ, Pastan I. IL2-PE664Glu, a new chimeric protein cytotoxic to human-activated T lymphocytes. J Biol Chem. 1990 Sep 25;265 (27):16311-7.

54. Batra JK, FitzGerald D, Gately M, Chaudhary VK, Pastan I. Anti-Tac(Fv)-PE40, a single

chain antibody Pseudomonas fusion protein directed at interleukin 2 receptor bearing cells. J Biol Chem. 1990 Sep 5;265 (25):15198-202.

55. Batra JK, Chaudhary VK, FitzGerald D, Pastan I. TGF alpha-anti-Tac(Fv)-PE40: a

bifunctional toxin cytotoxic for cells with EGF or IL2 receptors. Biochem Biophys Res Commun. 1990 Aug 31;171 (1):1-6.

56. Tomasselli AG, Hui JO, Sawyer TK, Staples DJ, Bannow CA, Reardon IM, Chaudhary VK,

Fryling CM, Pastan I, Fitzgerald DJ, et al. Proteases from human immunodeficiency virus and avian myeloblastosis virus show distinct specificities in hydrolysis of multidomain protein substrates. J Virol. 1990 Jul;64(7):3157-61.

57. Berger EA, Chaudhary VK, Clouse KA, Jaraquemada D, Nicholas JA, Rubino KL, Fitzgerald

DJ, Pastan I, Moss B. Recombinant CD4-Pseudomonas exotoxin hybrid protein displays HIV-specific cytotoxicity without affecting MHC class II-dependent functions. AIDS Res Hum Retroviruses. 1990 Jun;6(6):795-804.

58. Chaudhary VK, Batra JK, Gallo MG, Willingham MC, FitzGerald DJ, Pastan I. A rapid

method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins. Proc Natl Acad Sci U S A. 1990 Feb;87 (3):1066-70. Erratum in: Proc Natl Acad Sci U S A 1990 Apr;87(8):3253.

59. Tomasselli AG, Hui JO, Sawyer TK, Staples DJ, FitzGerald DJ, Chaudhary VK, Pastan I,

Heinrikson RL. Interdomain hydrolysis of a truncated Pseudomonas exotoxin by the human immunodeficiency virus-1 protease. J Biol Chem. 1990 Jan 5;265(1):408-13.

60. Chaudhary VK, Jinno Y, FitzGerald D, Pastan I. Pseudomonas exotoxin contains a specific

sequence at the carboxyl terminus that is required for cytotoxicity. Proc Natl Acad Sci U S A. 1990 Jan;87(1):308-12.

61. Siegall CB, Xu YH, Chaudhary VK, Adhya S, Fitzgerald D, Pastan I. Cytotoxic activities of a

fusion protein comprised of TGF alpha and Pseudomonas exotoxin. FASEB J. 1989 Dec;3(14):2647-52.

62. Berger EA, Clouse KA, Chaudhary VK, Chakrabarti S, FitzGerald DJ, Pastan I, Moss B.

CD4-Pseudomonas exotoxin hybrid protein blocks the spread of human immunodeficiency virus infection in vitro and is active against cells expressing the envelope glycoproteins from diverse primate immunodeficiency retroviruses. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9539-43. PubMed PMID: 2480605; PubMed Central PMCID: PMC298532.

63. Singh Y, Chaudhary VK, Leppla SH. A deleted variant of Bacillus anthracis protective

antigen is non-toxic and blocks anthrax toxin action in vivo. J Biol Chem. 1989 Nov 15;264(32):19103-7.

64. Batra JK, Jinno Y, Chaudhary VK, Kondo T, Willingham MC, FitzGerald DJ, Pastan I.

Antitumor activity in mice of an immunotoxin made with anti-transferrin receptor and a recombinant form of Pseudomonas exotoxin. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8545-9. PubMed PMID: 2510169; PubMed Central PMCID: PMC298319.

65. Jinno Y, Ogata M, Chaudhary VK, Willingham MC, Adhya S, FitzGerald D, Pastan I. Domain

II mutants of Pseudomonas exotoxin deficient in translocation. J Biol Chem. 1989 Sep 25;264(27):15953-9.

66. Siegall CB, Chaudhary VK, FitzGerald DJ, Pastan I. Functional analysis of domains II, Ib,

and III of Pseudomonas exotoxin. J Biol Chem. 1989 Aug 25;264(24):14256-61.

67. Chaudhary VK, Queen C, Junghans RP, Waldmann TA, FitzGerald DJ, Pastan I. A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin. Nature. 1989 Jun 1;339(6223):394-7.

68. Ogata M, Chaudhary VK, FitzGerald DJ, Pastan I. Cytotoxic activity of a recombinant fusion

protein between interleukin 4 and Pseudomonas exotoxin. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4215-9.

69. Siegall CB, Chaudhary VK, FitzGerald DJ, Pastan I. Cytotoxic activity of an interleukin 6-

Pseudomonas exotoxin fusion protein on human myeloma cells. Proc Natl Acad Sci U S A. 1988 Dec;85 (24):9738-42.

70. Chaudhary VK, Mizukami T, Fuerst TR, FitzGerald DJ, Moss B, Pastan I, Berger EA.

Selective killing of HIV-infected cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein. Nature. 1988 Sep 22;335 (6188):369-72.

71. Singh Y, Chaudhary VK, Tyagi SR, Bhatnagar R, Misra UK. Hepatic phosphatidylcholine

synthesis in deficiency of lysine and threonine: effect of malathion. Experientia. 1988 Sep 15;44(9):758-9.

72. Jinno Y, Chaudhary VK, Kondo T, Adhya S, FitzGerald DJ, Pastan I. Mutational analysis of

domain I of Pseudomonas exotoxin. Mutations in domain I of Pseudomonas exotoxin which reduce cell binding and animal toxicity. J Biol Chem. 1988 Sep 15;263(26):13203-7.

73. Gallo MG, Chaudhary VK, Fitzgerald DJ, Willingham MC, Pastan I. Cloning and expression of the H chain V region of antibody OVB3 that reacts with human ovarian cancer. J Immunol. 1988 Aug 1;141(3):1034-40.

74. Kondo T, FitzGerald D, Chaudhary VK, Adhya S, Pastan I. Activity of immunotoxins

constructed with modified Pseudomonas exotoxin A lacking the cell recognition domain. J Biol Chem. 1988 Jul 5;263 (19):9470-5.

75. Singh Y, Chaudhary VK, Bhatnagar R, Misra UK. Mixed function oxidases in response to

quality and quantity of dietary protein. Biochem Int. 1988 Jul;17 (1):1-8.

76. Chaudhary VK, Xu YH, FitzGerald D, Adhya S, Pastan I. Role of domain II of Pseudomonas exotoxin in the secretion of proteins into the periplasm and medium by Escherichia coli. Proc Natl Acad Sci U S A. 1988 May;85(9):2939-43.

77. Chaudhary VK, FitzGerald DJ, Adhya S, Pastan I. Activity of a recombinant fusion protein

between transforming growth factor type alpha and Pseudomonas toxin. Proc Natl Acad Sci U S A. 1987 Jul;84 (13):4538-42.

78. Chauhan SS, Chaudhary VK, Narayan S, Misra UK. Cytotoxicity of inhaled coal fly ash in

rats. Environ Res. 1987 Jun;43(1):1-12.

79. Grover SK, Chaudhary VK, Srivastava KK, Misra UK. An improved method for the isolation of human serum retinol binding protein (S-RBP). Indian J Biochem Biophys. 1986 Aug; 23(4):223-6

80. Srivastava PK, Chaudhary VK, Chauhan SS, Srivastava VK, Misra UK. Biochemical and

pathological effects of fly ash on lung, liver, and blood of rats. Arch Environ Contam Toxicol. 1984 Jul;13 (4):441-52.

81. Goyal KC, Sharma YK, Chaudhary VK, Misra UK. An improved method for isolation of

retinol-binding protein from goat serum. Indian J Biochem Biophys. 1984 Feb;21(1):59-61.

82. Singh Y, Chaudhary VK, Tyagi SR, Misra UK. Inhibition of hepatic phosphatidylcholine synthesis by malathion in rats. Toxicol Lett. 1984 Feb;20(2):219-23..

83. Chaudhary VK, Misra UK. Serum retinol binding protein secretion through microtubules in

chicks. Int J Vitam Nutr Res. 1980;50(4):422-3..