»õU÷hõì. - 162.241.27.72

wk 10

F–2587

B.Sc. DEGREE EXAMINATION, NOVEMBER 2019

First Semester

Biotechnology

BIOCHEMISTRY

(CBCS – 2017 onwards)

Time : 3 Hours Maximum : 75 Marks

Part A (10 2 = 20)

Answer all the questions.

1. Lactose.

»õU÷hõì.

2. Glycogen.

QøÍU÷Põáß.

3. Domain.

öhõø©ß.

4. Non-Essential amino acids.

Azv¯õÁ]¯ªÀ»õu Aª÷Úõ Aª»[PÒ.

5. Lipoproteins.

¼¨÷£õ¦μu[PÒ.

6. Fats.

öPõÊ¨¦PÒ.

Sub. Code 7BBT1C1

F–2587

2

Wk 107. mRNA.

G®.Bº.Gß.H.

8. Phosphodiester bond.

£õì÷£õøhö¯ìhº ¤øn¨¦.

9. Hydrolases.

øím÷μõ÷»ìPÒ.

10. Enzyme units.

ö|õv°ß A»SPÒ.

Part B (5 5 = 25)

Answer all the questions, choosing either (a) or (b).

11. (a) Write a short notes on structure of glucose.

SÐU÷Põêß Aø©¨¦ £ØÔ ]Ö SÔ¨¦ ÁøμP.

Or

(b) Write about the biological importance of the starch.

ìhõº]ß E°› •UQ¯zxÁ® £ØÔ GÊxP.

12. (a) Give a short notes on chemical properties of amino acids.

Aª÷Úõ Aª»[PÎß ÷Áv¨ £s¦PøÍ £ØÔ ]Ö

SÔ¨¦ ÁøμP.

Or

(b) Write a note on non-essential amino acids.

Azv¯Áõ]¯ªÀ»õu Aª÷Úõ Aª»[PÒ £ØÔ SÔ¨¦

ÁøμP.

F–2587

3

Wk 1013. (a) Outline the functions of Phospholipids.

£õì÷£õ¼¤miß ö\¯À£õkPøÍ GÊxP.

Or

(b) List out the functions of Glycolipids.

QøÍ÷Põ¼¤mêß ö\¯À£õkPøÍ £mi¯¼kP.

14. (a) Write a short notes on composition of DNA.

i.Gß.H Âß ÷\º©õÚ[PÒ ©ØÖ® Pmhø©¨¤øÚ

£ØÔ¯ ]Ö SÔ¨¦ ÁøμP.

Or

(b) Explain the structure and functions of tRNA.

i.Gß.H.Âß Aø©¨¦ ©ØÖ® ö\¯À£õmiøÚ Â›ÁõP

GÊxP.

15. (a) Outline the classification of enzymes.

ö|õvPÎß ÁøP¨£õmiøÚ Â›ÁõP GÊxP.

Or

(b) Write in brief about lock and key hypothesis.

§mk ©ØÖ® Âø\°ß P¸x÷PõÒ £ØÔ Â›ÁõP

GÊxP.

Part C (3 10 = 30)

Answer any three questions.

16. Write an essay about polysaccharides.

£õ¼\õUøμmPøÍ £ØÔ¯ Kº Pmkøμ GÊuÄ®.

17. Describe in detail about the levels of structural organisation of proteins.

¦μuzvß Pmhø©¨¤ß {ø»PøÍ £ØÔ ÂÍUSP.

F–2587

4

Wk 1018. Outline the structure and properties of fatty acids.

öPõÊ¨¦ Aª»[PÎß Aø©¨¦ ©ØÖ® Auß £s¦PøÍ

ÂÍUSP.

19. Explain in detail about Watson and crick model of DNA.

Áõm\ß ©ØÖ® Q›U Psk¤izu iGßH–Âß ‰»UTÖ £ØÔ

Â›ÁõP GÊxP.

20. List out the factors affecting the enzyme activity.

ö|õvPÎß ö\¯À£õmiøÚ £õvUS® PõμoPøÍ

£mi¯¼kP.

——————

Ws9

F–2588


Second Semester

Biotechnology

MICROBIOLOGY



Part A (10 2 = 20)


1. Mycoplasma

ø©U÷Põ¨Íõì©õ

2. Agricultural microbiology

÷ÁÍõs ~sq°›¯À

3. Robert Hooke

μõ£ºm íúU

4. Magnification

E¸¨ö£¸UP®

5. Lithotrophs

¼z÷uõm÷μõ¨ì

6. Capsalar staining

ö£õv²øÓ \õ¯÷©ØÓÀ

Sub. Code 7BBT2C1

F–2588

2

Ws9

7. Flagellam

Pø\°øÇ

8. Virion

Â›¯õß

9. Superbug

£°ÀÁõß Q¸ªPÒ

10. Bioleaching

£÷¯õ½a][

Part B (5 5 = 25)


11. (a) Outline the importance of Microbiology.

~sq°›¯¼ß •UQ¯zxÁzøu _¸UQ GÊxP.

Or

(b) Explain the different branches of Microbiology.

~sq°›¯¼ß £»ÁøP¯õÚ ¤›ÄPøÍ ÂÍUSP.

12. (a) Outline the parts of a microscope with a diagram.

~s÷nõUQ¯ß £õPzvøÚ Áøμ¨£h•hß _¸UQ

GÊxP.

Or

(b) Explain the applications of Dark-field microscope.

ÇÒ `Ì¢u ~s÷nõUQ°ß £¯ßPøÍ ÂÍUSP.

F–2588

3

Ws9

13. (a) Describe the cultivation strategy of Anaerobic organisms.

A÷Úõ÷μõ¤U ~sq°›PÎß EØ£zv

AqS•øÓø¯ ÂÍUSP.

Or

(b) Outline the characteristics of Autotrophs.

ußÞmh E°›°ß £s¤¯ÀPøÍ _¸UQ GÊxP.

14. (a) Explain the ultrastructure of a typical bacterial cell.

J¸ £õUj›¯õ ö\À¼ß ©õv› ~s Aø©¨¤øÚ

ÂÍUSP.

Or

(b) Outline the basic characteristics of fungi.

§gø\°ß Ai¨£øh £s¦PøÍ _¸UQ GÊxP.

15. (a) Write the importance of microorganisms present in water.

usp›À ÇUPUTi¯ |sq°›PÎß

•UQ¯zxÁzøu GÊxP.

Or

(b) Explain the types and properties of Archeabacteria.

BºQ°Uj›¯õÂß ÁøPPÒ ©ØÖ® £s¦PøÍ

ÂÍUSP.

Part C (3 10 = 30)


16. Describe the Bergey's classification of bacteria.

ö£ºUR]ß £õUj›¯À ÁøPPøÍ ÂÍUQ GÊxP.

F–2588

4

Ws9

17. Write an essay about Electron Microscope with special reference to scanning electron microscope.

G»Umμõß ~s÷nõUQ GßÝ® uø»¨¤À J¸ Pmkøμ

GÊv. AvÀ •UQ¯©õP ìPõÛ[ G»Umμõß

~s÷nõUQø¯ ÂÍUQ GÊuÄ®.

18. Differentiate the bacteria cell by using gram staining reagents.

Qμõ® {Ó÷©ÖuÀ ÷Ávö£õ¸ÒPÒ EuÂ ‰»®

£õUj›¯õUPøÍ ÷ÁÖ£kzx® •øÓPøÍ GÊxP.

19. Outline the structure and functions of cytoplasmic membrane.

E°μq ö©ß\ÆÂß Aø©¨¦ ©ØÖ® ö\¯À£õkPøÍ _¸UQ

GÊxP.

20. Write the importance and strategies used in the field of environmental biotechnology.

_ØÖa`ÇÀ E°›¯¼À øP¯õÍ¨£k®

•UQ¯zxÁzvøÚ²® AqS•øÓPøÍ²® GÊuÄ®

————————

WSS

F–2589


Third Semester

Biotechnology

CELL AND MOLECULAR BIOLOGY



Section A (10 2 = 20)


1. Define Cell.

ö\À £ØÔ ÂÁ›.

2. Explain about cell theory.

E°μqUöPõÒøP £ØÔ ÂÍUP® u¸P.

3. What is cell division?

ö\À ¤›Ä GßÓõÀ GßÚ?

4. What is cell synchrony?

ö\À Jzvø\Ä GßÓõÀ GßÚ?

5. Explain about golgi bodies.

PõÀQ £õiì £ØÔ ÂÍUSP.

6. Explain about Endoplasmic reticulum.

Gs÷hõ¤ÍõìªU öμiSÍ® £ØÔ ÂÍUSP.

Sub. Code 7BBT3C1

F–2589

2

WSS

7. What is replication?

¤μvö\´øP GßÓõÀ GßÚ?

8. Enzymes involved in DNA replication.

iGßH ¤μvö\´øP°À £[Sö£Ö® ö|õvPøÍ SÔ¨¤kP.

9. What is transcription?

£iö¯kzuÀ GßÓõÀ GßÚ?

10. What is translation?

¦μuEØ£zv GßÓõÀ GßÚ?

Section B (5 5 = 25)


11. (a) Give a detailed note on classification of cell types.

ö\À¼ß ÁøPPøÍ ÂÍUP©õP ÁøP¨£kzxP.

Or

(b) Explain about different levels of organisation of cell.

ö\À¼ß Aø©¨¤øÚ £ØÔ öÁÆ÷ÁÖ {ø»PÎÀ

ÂÁ›.

12. (a) Explain in detail about cell cycle.

ö\À _ÇØ] £ØÔ Â›ÁõP GÊxP.

Or

(b) Write a short note on cell synchrony and its applications.

ö\À Jzvø\Ä ©ØÖ® Auß £¯ß£õmiøÚ £ØÔ

ÂÍUSP.

F–2589

3

WSS

13. (a) Explain in detail about organization and functions of cytoskeletons.

ö\À \mhPzvß Aø©¨¦ ©ØÖ® ö\¯À£õmiøÚ

ÂÍUSP.

Or

(b) Give a note on organization and functions of nucleus.

P¸Âß Aø©¨¦ ©ØÖ® ö\¯À£õmiøÚ ÂÍUSP.

14. (a) Explain about central dogma of molecular biology.

‰»UTØÖ E°›¯¼ß ø©¯U÷Põm£õmiøÚ ÂÍUSP.

Or

(b) Give a note on enzymes and proteins involved in DNA replication.

©μ¦ CøÇ £iö¯kzu¼À £[Sö£ÓUTi¯ ö|õvPøÍ

£ØÔ²® ¦μu[PøÍ £ØÔ²® ÂÁ›.

15. (a) Explain in detail about mechanism of translation in prokaryotes.

¦÷μõ÷P›÷¯õmêÀ {PÊ® ¦μuEØ£zv°ß

ö£õÔ•øÓø¯ ÂÍUSP.

Or

(b) Explain in detail about mechanism of translation in eukaryotes.

³UP›÷¯õmhõPÎÀ {PÊ® ¦μuEØ£zv°ß

ö£õÔ•øÓø¯ ÂÍUSP.

F–2589

4

WSS

Section C (3 10 = 30)


16. Elaborate the structure of prokaryotic and eukaryotic cell.

¦÷μõ÷Pº÷¯õmiU ©ØÖ® ³UPõº÷¯õmiU ö\À Aø©¨ø£

£ØÔ ÂÁ›UPÄ®.

17. Explain in detail about mitotic and meiotic cell division.

ø©mhõêì ©ØÖ® ª¯õmiU ö\À ¤›ÂøÚ £ØÔ

ÂÍUP©õP ÂÁ›UPÄ®.

18. Explain about structure and functions of cell organelles.

ö\À ö£õ¸ÒPÎß Aø©¨¦ ©ØÖ® ö\¯À£õmiøÚ²® £ØÔ

ÂÁ›UPÄ®.

19. Write a essay on models of replication.

¤μvPÎß •øÓPøÍ £ØÔ Pmkøμ ÁøμP.

20. Give a detailed note on prokaryotic and eukaryotic transcription.

¦÷μõ÷Pº÷¯õmiU ©ØÖ® ³U÷Pº÷¯õmiU £iö¯k¨¦ £ØÔ

Â›ÁõP ÂÍUP® u¸P.

————————

WSS

F–2590


Fourth Semester

Biotechnology

PRINCIPLES OF GENETICS



Part A (10 2 = 20)


1. Genotype.

©μ£q Aø©¨¦.

2. Dihybrid cross.

Cμmøh¨ £s¦ CÚUP»¨¦.

3. Allosome.

£õÀ\õº¢u S÷μõ÷©õ÷\õ®.

4. Pleiotrophic genes.

¤Î÷¯õm÷μõ¤U ãßPÒ.

5. Translocation.

ãßPÒ Ch©õØÓ®.

6. Down’s syndrome.

hÄßì ]ßmöμõ®.

Sub. Code 7BBT4C1

F–2590

2

WSS

7. Haemophilia.

Cμzu JÊUS÷|õ´.

8. Natural selection.

C¯ØøP ÷uºÄ.

9. Lytic cycle.

]øuUS® _ÇØ].

10. Jumping genes.

uõÄQßÓ ãßPÒ.

Part B (5 5 = 25)


11. (a) Explain briefly about the law of dominance.

K[S® Âv°øÚ £ØÔ Â›ÁõP ÂÍUSP.

Or

(b) Write about the father of genetics.

©μ¤¯¼ß u¢øuø¯ £ØÔ GÊxP.

12. (a) Explain incomplete dominance with an example.

{øÓÄÓõ BÐø©ø¯ J¸ Euõμnzxhß ÂÍUSP.

Or

(b) What do you mean by sex linkage?

£õÀ CønÄ £ØÔ }Âº AÔÁÚ ¯õøÁ?

13. (a) Describe Euploidy with a suitable example.

³¨Íõíø¯ ö£õ¸zu©õÚ GkzxUPõmkhß

ÂÍUSP.

Or

F–2590

3

WSS

(b) Write short notes on chromosomal duplication.

S÷μõ÷©õ÷\õ® Cμmi¨£øhuÀ £ØÔ¯ ]Ö SÔ¨¦

ÁøμP.

14. (a) Explain sex-linked inheritance in human beings.

©ÛuS»zvÀ Põn¨£k® £õ÷»õk ¤øn¢u

£õμ®£›¯zøu ÂÍUSP.

Or

(b) Write an account on Pedigree analysis.

£μ®£øμ B´øÁ £ØÔ¯ SÔ¨¦ ÁøμP.

15. (a) Explain how transformation occur in Prokaryotes.

¨÷μõ÷Pº÷¯õmkPÒ ö\À¼À |øhö£Ö® {ø»©õØÓ®

G¨£i |hUQÓx Gß£uøÚ ÂÍUSP.

Or

(b) Describe Davis U tube experiment.

÷hÂì U SÇõ´ ÷\õuøÚø¯ ÂÍUSP.

Part C (3 10 = 30)


16. Explain Mendel’s law of segregation with a suitable example.

ö©sh¼ß uÛ¨£kzuÀ Âvø¯ uS¢u Euõμnzxhß

ÂÍUSP.

17. Write an account on co-dominance and its importance.

Cøn K[Sz ußø©ø¯²® Auß •UQ¯zxÁzøu²®

GÊuÄ®.

F–2590

4

WSS

18. Explain Aneuploidy with suitable examples.

©μ¦zv› GsoUøP ©õØÓzvøÚ uS¢u GkzxUPõmkhß

ÂÍUSP.

19. Write an essay about population genetics.

E°ºzöuõøP £õμ®£›¯Â¯À £ØÔ¯ Kº Pmkøμ GÊxP.

20. Outline the steps involved in lysogenic cycle.

ø»÷\õöáÛ _ÇØ]°À £[SöPõÒÐ® £iPøÍ _¸UQ

GÊx.

————————

Ws8

F–2591


Fifth Semester

Biotechnology

PRINCIPLES OF IMMUNOLOGY



Part A (10 2 = 20)


1. Blood transfusion

S¸v÷¯ØÓÀ

2. Bone marrow

G¾®¦ ©äøá

3. Antigens

BßiöáßPÒ

4. IgG

IgG

5. Agglutination

AUÐmi÷Úåß

6. Electrophoresis

ªß¤›øP

7. Macrophages

÷©U÷μõ÷£ãPÒ

Sub. Code 7BBT5C1

F–2591

2

Ws8

8. B – Cells

B – ö\ÀPÒ

9. DBT Vaccine

i¤i uk¨§]

10. Asthma

Bìx©õ

Part B (5 5 = 25)


11. (a) Explain the basic factors involved in innate immunity.

C¯ØøP Gvº¨¦\Uv°À £[S öPõÒÐ® Ai¨£øh

PõμoPøÍ ÂÍUSP.

Or

(b) Outline the scope of immunology.

÷|õö¯vº¨¤¯À £õhzvß ÷|õUPzvøÚ _¸UQ

GÊxP.

12. (a) Differentiate immunogenicity and antigenicity

C®•÷ÚõöáÛ]mi ©ØÖ® BßiöáÛ]mi°ß

÷ÁÖ£õkPÒ GÊxP.

Or

(b) Explain the salient features of Haptens.

íõ¨hßPÎß uÛ¨£s¦PøÍ ÂÍUSP.

F–2591

3

Ws8

13. (a) Outline the Principle of Immunodiffusion.

C®•÷Úõi¨³\Ûß ÷Põm£õiøÚ _¸UQ GÊxP.

Or

(b) Explain the details about ELISA.

G¼\õ £ØÔ¯ ö£¸SÔ¨¦ ÁøμP.

14. (a) What is complement and write its functions?

P®¤Îö©ßm GßÓõÀ GßÚ? ©ØÖ® AuÛß

ö\¯À£õkPÒ £ØÔ GÊxP.

Or

(b) Explain the class I and class II MHC molecules.

Q»õì I ©ØÖ® Q»õì II G®.Ga.] ‰»UTÖPøÍ

ÂÍUSP.

15. (a) Outline the types of Active Immunity.

BUiÆ ÷|õ´ Gvº¨¦ ÁøPPøÍ _¸UQ GÊxP.

Or

(b) Explain briefly the types of hypersensitivity.

EnºvÓß ªøP¨¦ ÁøPPøÍ _¸UQ GÊxP.

Part C (3 10 = 30)


16. Outline the functions of cells in the immune system.

÷|õö¯vº¨¦ ©sh»zvÀ EÒÍ ö\ÀPÎß ö\¯À£õkPøÍ

_¸UQ GÊxP.

17. Explain the structure and functions of Antibodies.

Bßi£õiPÎß Pmhø©¨¦ ©ØÖ® ö\¯À£õkPøÍ

ÂÍUSP.

F–2591

4

Ws8

18. Describe the principle and applications of Blotting techniques.

¤Íõmi[ öuõÈÀ~m£zvß öPõÒøP ©ØÖ® £¯ß£õkPøÍ

ÂÍUSP.

19. Explain cystolic and endocytic pathways.

]ìhõ¼U ©ØÖ® Gß÷hõø»miU £õøuPøÍ ÂÍUSP.

20. Write an essay about Passive Immunization.

ö\¯»ØÓ ÷|õ´zuk¨¦ •øÓ £ØÔ¯ J¸ Pmkøμ GÊxP.

————————

Wk8

F–2592


Fifth Semester

Biotechnology

MICROBIAL BIOTECHNOLOGY



Part A (10 2 = 20)

Answer all questions.

1. Transformation of gene.

©μ£q ©õØÓ®

2. Natural media

C¯ØøP FhP®

3. Aerator

PõØÖ KØÔ

4. Fermentation

ö|õvzuÀ

5. TLC

i.GÀ.]

6. Sonication

J¼ ‰»® ]øu¨¦

Sub. Code 7BBT5C2

F–2592

2

Wk87. Yeast

Dìm

8. Super bag

`¨£º £U

9. Yoghurt

u°º

10. Metabolites

ÁÍº]øu ©õØÓ¨ ö£õ¸mPÒ

Part B (5 5 = 25)

Answer all questions, Choosing Either (a) or (b).

11. (a) Write brief notes on secondary screening of micro organisms?

~sq°›PøÍ ÷uºÄ ö\´¯ EuÄ® Cμshõ® {ø»

÷uºÄ £ØÔ Â›ÁõP GÊxP.

Or

(b) Mention the role of rDNA technology for the improvements of strains.

i.Gß.H ©Ö÷\ºUøP öuõÈÀ~m£zvß ‰»®

~sq°›PøÍ GÆÁõÖ ÷©®£kzu»õ® £ØÔ

SÔ¨¤kP.

12. (a) Explain the principles of HEPA filters.

öí¨£õ ÁiPmi°ß uzxÁ® £ØÔ ÂÁ›.

Or

(b) Give an account on solid state fermentation.

vh{ø» ö|õvzuÀ £ØÔ SÔ¨¦ u¸P.

F–2592

3

Wk813. (a) Write brief notes on concentration of products.

ö\ÔÅmh¨£mh ö£õ¸mPøÍ¨ £ØÔ Â›ÁõP SÔ¨¦

GÊxP.

Or

(b) Point out the principles of shake flask fermentation.

Aø\²® SkøÁ ö|õvzuÀ uzxÁzvøÚ _mi Põmk.

14. (a) Describe any one method of gene cloning in yeast.

DìiÀ ãß S÷ÍõÛ[ ö\´²® HuõÁx J¸

öuõÈÀ~m£® £ØÔ ÂÁ›.

Or

(b) Explain the different types of bioremediation.

£÷¯õöμªi÷¯\Ûß ÷ÁÖ£mh {ø»PÒ £ØÔ ÂÁ›.

15. (a) How yeast biomass can be produced?

Dìm £÷¯õ©õì GÆÁõÖ EØ£zv ö\´¯¨£kQÓx.

Or

(b) Explain the methodology of Ethyl alcohol production.

GzvÀ BÀPíõÀ EØ£zv ö\´²® öuõÈÀ ~m£® £ØÔ

ÂÁ›.

Part C (3 10 = 30)


16. Write an essay on isolation of microorganisms.

~sq°›PøÍ uÛø©£kzxuÀ £ØÔ Pmkøμ GÊxP.

17. Explain the principles and applications of continuous fermentation.

öuõhº{ø» ö|õvzu¼ß uzxÁ® ©ØÖ® £¯ß£õkPÒ £ØÔ

ÂÁ›.

F–2592

4

Wk818. Explain in detail about the Down stream processing.

RÌ{ø» ö\¯»õUP® £ØÔ Â›ÁõP ÂÁ›.

19. Discuss about the management of waste by using pseudomonas – sp.

PÈÄ ÷©»õsø© ö\´ÁvÀ `÷hõ÷©õÚõì £[S £ØÔ

öu›ÂUP.

20. Give neat sketch on the production of penicilin.

ö£Û]¼ß EØ£zv £ØÔ öuÎÁõP SÔ¨¤kP.

——————

Wk 6

F–2594


Fifth Semester

Biotechnology

Elective — BIOPHYSICS



Part A (10 2 = 20)


1. Define RF value.

RF & Áøμ¯øÓ.

2. What is radio active isotope?

÷μi÷¯õ BUiÆ I÷\õ÷hõ¨ GßÓõÀ GßÚ?

3. Define half life.

Aøμ B²mPõ»® & Áøμ¯øÓ.

4. What is Agarose?

APõº GßÓõÀ GßÚ?

5. What is the principle of spectroscopy?

{Ó©õø»©õÛ°ß uzxÁ® £ØÔ GÊxP.

6. Define the term PAGE.

PAGE £ØÔ Áøμ¯øÓ.

Sub. Code 7BBTE1B

F–2594

2

Wk 67. IR spectroscopy.

APa]Á¨¦ {Ó©õø»©õÛ.

8. What is chromatogrpahy?

{μa\õÓÀ GßÓõÀ GßÚ?

9. PCR.

¤.].Bº.

10. What is the application of gene sequencing?

©μ£q Á›ø\°ß £¯ß£õkPÒ GßÚ?

Part B (5 5 = 25)


11. (a) Discuss the function of autoradiography.

uØPvº¨£vÄ &ß ö\¯À£õkPÒ £ØÔ ÂÁ›.

Or

(b) Point out the importance of radioisotope in biology.

Radioisotope ß E°›¯À •UQ¯zxÁ® £ØÔ _miU

PõmkP.

12. (a) Give a brief notes on UV spectroscopy.

¦Ó Fuõ {Ó©õø» Põmi £ØÔ _¸UP©õP SÔ¨¦ u¸P.

Or

(b) Write principle and application of NMR.

NMR øμß uzxÁ® ©ØÖ® £¯ß£õkPÒ £ØÔ B´P.

F–2594

3

Wk 613. (a) Describe the briefly about vertical gel

electrophoresis.

Vertical gel electrophoresis £ØÔ _¸UP©õP ÂÍUSP.

Or

(b) Discuss the principle and application of SDS-PAGE.

SDS-PAGE ß uzxÁ® ©ØÖ® £¯ß£õkPÒ £ØÔ

ÂÁ›.

14. (a) Write short notes on gel filteration chromatography.

TÌ© ÁiPmhÀ {Ó B´Â¯À £ØÔ _¸UPU SÔ¨¦

GÊxP.

Or

(b) Discuss briefly the principle and application of HPLC.

HPLC&ß uzxÁ® ©ØÖ® £¯ß£õkPÒ £ØÔ ÂÁ›UP.

15. (a) Write brief notes on Sanger method of sequencing.

Sanger •øÓ £ØÔ _¸UP©õP SÔ¨¦ GÊxP.

Or

(b) Give detail account on PCR.

PCR ø¯¨ £ØÔ Â›ÁõÚ öuõS¨¦ u¸P.

Part C (3 10 = 30)


16. Describe the principle, procedure and application of GM counter.

GM counter&ß uzxÁ®, ö\´•øÓ ©ØÖ® £¯ß£õkPÒ

£ØÔ ÂÍUSP.

F–2594

4

Wk 617. Explain the principle and application of Mass

Spectroscopy.

Aq¨ ö£õ¸sø© Aø»©õø»°ß uzxÁ® ©ØÖ®

£¯ß£õkPÒ £ØÔ ÂÍUSP.

18. Describe the working methodology and applications of Immuno electrophoresis.

Immuno electrophoresis &ß ö\¯À£k® •øÓ ©ØÖ®

£¯ß£õkPÒ £ØÔ ÂÁ›.

19. Give detail account on gas-liquid chromatography.

Gas-liquid chromatography £ØÔ Â›ÁõÚ öuõS¨¦ u¸P.

20. Explain detail account on ELISA.

G¼\õ £ØÔ Â›ÁõÚ ÂÍUP® u¸P.

————————

WSS

F–2596


Fifth Semester

Biotechnology

Elective — ECOLOGY AND EVOLUTION



Part A (10 2 = 20)


1. Define Piokilothermic.

÷£õÚQ÷»õöuºªU&I Áøμ¯ÖUPÄ®.

2. Write note on Carnivores.

FÝsoPÒ £ØÔ SÔ¨¦ GÊxP.

3. Give short note on Mutualism.

£›©õØÖ ÁõÌÄ £ØÔ ]Ö SÔ¨¦ GÊxP.

4. Classify the parasites with example.

Euõμn[PÐhß JmksoPøÍ ÁøP¨£kzuÄ®.

5. Define Lithosphere.

¼z÷uõì¤¯øμ Áøμ¯ÖUPÄ®.

6. Discuss about phagotrophs.

£õ÷Põm÷μõ¨ì £ØÔ ÂÁõvUPÄ®.

Sub. Code 7BBTE2B

F–2596

2

WSS

7. Phylogenetic patterns.

ø£÷»õöáöÚiU •øÓPÒ.

8. Write note on closed circulatory system.

‰i¯ Cμzu Kmhzvß SÔ¨¦ GÊxP.

9. What is Geographical isolation?

¦Â°¯À uÛø© GßÓõÀ GßÚ?

10. Write about Homogamy.

\©ÂÚ¨¦nºa] £ØÔ GÊxP.

Part B (5 5 = 25)


11. (a) Define the term ecology and describe about the significance of ecology.

`Ç¼¯ø» Áøμ¯ÖUPÄ® ©ØÖ® Auß •UQ¯zxÁ®

£ØÔ ÂÁ›UPÄ®.

Or

(b) Explain about the pond ecosystem with examples.

Euõμn[PÐhß SÍ® _ØÖa`ÇÀ Aø©¨¦ £ØÔ

ÂÍUS[PÒ.

12. (a) Explain Commensalism and Antagonism.

P®ö©ß\¼\® ©ØÖ® Ash÷PõÛ\®&I ÂÍUPÄ®.

Or

(b) Write about the intraspecific interactions.

]ØÔÚzuP CøhÂøÚPÒ £ØÔ GÊuÄ®.

F–2596

3

WSS

13. (a) List out the difference between Troposphere and Stratosphere.

AiöÁÎ¨£Sv ©ØÖ® AkUS©sh»zvß Cøh°À

EÒÍ ÷ÁÖ£õkPøÍ £mi¯¼k.

Or

(b) Describe the zones of marine ecosystem.

PhÀ `ÇÀ ©sh»[PÒ £ØÔ ÂÁ›UPÄ®.

14. (a) Explain about the geographical distribution of organisms.

E°›Ú[PÎß ¦Â°¯À £μÁÀ £ØÔ ÂÍUS[PÒ.

Or

(b) Write about the anatomical anomalies.

EhØTÔ¯À •μs£õkPÒ £ØÔ GÊxP.

15. (a) Write the difference between dimorphism and polymorphism.

øh©õº¤é® ©ØÖ® £õ¼©õº¤é® Cøh°À EÒÍ

÷ÁÖ£õkPøÍ GÊxP.

Or

(b) Discuss about the neutral mutation with example.

Euõμn[PÐhß ~²mμÀ ¤ÓÌÄ £ØÔ ÂÁõvUPÄ®.

Part C (3 10 = 30)


16. Describe the role of temperature as an important ecological factors.

J¸ •UQ¯ _ØÖa`ÇÀ PõμoPÍõP öÁ¨£{ø»°ß £[øP

ÂÁ›UPÄ®.

F–2596

4

WSS

17. Write about the factors regulating population size elaborately.

©UPÒöuõøP AÍøÁU Pmk¨£kzx® PõμoPøÍ¨ £ØÔ

Â›ÁõP GÊuÄ®.

18. What are biomes? And describe the different types of it.

E°›¯[PÒ GßÓõÀ GßÚ? ©ØÖ® AÁØÔß £À÷ÁÖ

ÁøPPøÍ ÂÁ›UPÄ®.

19. Explain the theory of Lamarck.

ö»®©õºU ÷Põm£õmøh ÂÍUS[PÒ.

20. Discuss about the distribution of species.

CÚ[PÒ Â{÷¯õP® £ØÔ ÂÁõvUPÄ®.

————————

»õU÷hõì. - 162.241.27.72

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