METABOLIZAM UGLJIKOHIDRATA METABOLIČKI PUTEVI
Oct 24, 2014
METABOLIZAM UGLJIKOHIDRATA
METABOLIČKI PUTEVI
Shematski prikazkatabolizma
Složeni metaboliti kao što suugljikohidrati, masti i proteinirazgrađuju se prvo do namonomerne jedinice (većinomglukozu, aminokiseline, masnekiseline i glicerol), a zatim doacetil-CoA koji ulazi u cikluslimunske kiseline.
Lactose intolerance
Fate of Galactose and Fructose
LIVER
fructose
galactose
fructose
galactose
glucose glucose
BLOOD
Absorption of glucose from the blood:
Facilitated diffusionGlucose Transporters (GLUT)
GLUT-1 through GLUT-5
TRANSPORT GLUKOZE U STANICU
1. OLAKŠANI TRANSPORT- uz transportere GLUT1-5, čiju aktivnost povećava INZULIN- npr. GLUT-4 je prisutan u adipoznom tkivu i skeletnim mišićima, a GLUT-1 u eritrocitima
- konc. Glu izvan stanice jepovećana
Glucose Uptake by Musclesand Fat Cells
TRANSPORT GLUKOZE U STANICU
2. KOTRANSPORT
-“protiv” koncentracijskog gradijenta- uz istovremeni transport Na+
-odvija se u intestinalnim epitelnim stanicama, te u bubrežnim tubulima
Metabolički putevi ugljikohidrata
• GLIKOLIZA• GLUKONEOGENEZA• CIKLUS PENZOZA FOSFATA
– oksidativni put– neoksidativni put
• GLIKOGENOLIZA• GLIKOGENEZA
GLIKOLIZA
Oksidativna razgradnja glukoze-6-fosfata do
piruvata
GLIKOLIZA
•AEROBNA GLIKOLIZA(glukoza-piruvat-acetil CoA-CLK-RL-ATP)
•ANAEROBNA GLIKOLIZA(glukoza-piruvat-laktat)
Heksokinaza
Pregled ključnih reakcija i enzima glikolize i glukoneogeneze
Glukoza-6-fosfataza
Fruktokinaza
Piruvat kinaza
Fruktoza-1,6-bisfosfataza
Fosfoenol-piruvat karboksikinaza
Piruvat-karboksilaza
AEROBIC ANAEROBIC
glucose
2 pyruvate
2 acetyl CoA
TCA
glucose
2 pyruvate 2 lactate
Minor Source of ATP
Minor Source of ATP
Major Source of ATP
ONLY Source of ATP
1. Traps glucose inside cells
2. Priming reaction:
Glucose + Pi Glucose-6-P + H2O +3300
ATP + H2O ADP + Pi -8000
Glucose + ATP Glucose-6-P + ADP -4700
3. Irreversible-4700
COO
C
CH3
O2 pyruvate
2 CoASH
2 CO2
2 NAD+
2 NADH + H+
H3C C S
O
CoA2
acetyl CoA
2 NADH + H+
2 NAD+
lactate dehydrogenase
pyruvate dehydrogenase
COO
CH
CH3
2 OH
lactate
1112
Why Lactate is Formed?
glyceraldehyde-3-phosphate
glyceraldehyde-3-P dehydrogenase
1,3-bisphosphoglycerate
ADP
ATP
3-phosphoglycerate
2-phosphoglycerate
phosphoenolpyruvate
pyruvate
ADP
ATP
NAD+
NADH + H+ETS(aerobic conditions only)
Under anaerobicconditions the ETSdoesn’t work
lactatelactate dehydrogenase
Zašto nastaje laktat
U anaerobnim uvjetima ne djeluje respiratorni lanac
Cori Cycle
Exercising musclesalso generate lactate
1
2
3
4
IV. CARBOHYDRATE METABOLISMA. Digestion and absorptionB. Glycolysis
1. General characteristics2. Individual reactions3. Lactic dehydrogenase4. Pyruvate oxidation5. Energetics
COO
C
CH3
O2 pyruvate
2 CoASH
2 CO2
2 NAD+
2 NADH + H+
H3C C S
O
CoA2
acetyl CoA
2 NADH + H+
2 NAD+
lactate dehydrogenase
pyruvate dehydrogenase
COO
CH
CH3
2 OH
lactate
1112
Pyruvate Oxidation
H3C COO
Opyruvatedehydrogenase
NAD+ NADH + H+
H3C SCoA
O
+ CO2
CoASH
Pyruvate Dehydrogenase is a multienzyme complex(like alpha ketoglutarate dehydrogenase)
Consists of 3 enzymes
Requires 5 coenzymes: TPP, coenzyme A,
lipoic acid, FAD and NAD+
Regulation of PyruvateDehydrogenase
pyruvate
pyruvate
glucose
CYTOSOL
MITOCHONDRIA
pyruvate dehydrogenase
acetyl CoA
OAA citrate
CO2
ATP
ADP + Pi
pyruvatecarboxylase
Fatty Acids
TCA
Stimulated by ADP
Inhibited by:
ATP, acetyl CoA andexcess fatty acids
Stimulated byAcetyl CoA
Energetics of GlycolysisAEROBIC ANAEROBIC
glucose
2 pyruvate
2 acetyl CoA
TCA
glucose
2 pyruvate 2 lactate
2 ATP
2 NADHETS
6 ATP
2 ATP- 2 ATP
2 NADHETS
6 ATP
4 ATP(SLP) 4 ATP
12 ATP x 2 = 24 ATP
-2 ATP
NADH
NAD+
4 ATP 4 ATP
NET 38 ATP
2 ATP NET
GLUKONEOGENEZA
SINTEZA GLUKOZE IZ SPOJEVA KOJI NISU UGLJIKOHIDRATI
Glucose
ATP
ADP
glucose-6-P
H2O
Pi
fructose-6-P
ATP
ADP H2O
Pi
ftuctose-1,6-bisphosphate
dihydroxyacetone-P
glyceraldehyde-3-P
2 Pi
2 Pi
2 1,3-bisphosphoglycerate
2 ATP
2 ADP
2 ATP
2 ADP
2 3-phosphoglycerate
2 2-phosphoglycerate
2 phosphoenolpyruvate
2 pyruvate
2 oxaloacetate
2 ADP
2 ATP
2 ATP
2 ADP + 2Pi
2 GTP
2 GDP
2 lactatelactate dehydrogenase
2 NAD+
2 NADH
1
2
3
4
1
2
3
4
Pyruvatecarboxylase
PEP carboxy- kinase
Fructose-1,6-bisphosphatase
Glucose-6-phosphatase
1. Piruvat karboksilaza
2. Fosfoenol-piruvat karboksikinaza
2 KLJUČNA ENZIMA:
•PIRUVATKARBOKSILAZA
•PEP (FOSFOENOLPIRUVAT) KARBOKSIKINAZA
Cori Cycle
Exercising musclesalso generate lactate
1
2
3
4
CIKLUS PENTOZA FOSFATA
(PPP,CPF)
Overview of the PentosePhosphate Pathway
glucose-6- P
glucose
OXIDATIVE PHASE
2 NADP+ 2 NADPH
fatty acid synthesis
steroid synthesis
glutathione reduction
ribulose-5- P + CO2
INTERCONVERSION PHASE
glyceraldehyde-3- P
Oxidatively decarboxylatesglucose-6-P to ribulose-5-P
Converts ribulose-5-P back toglucose-6-P
METABOLIZAM GLIKOGENA
1. GLIKOGENEZA
SINTEZA GLIKOGENA
(UTP)
2 Pi
GLIKOGENOLIZA
RAZGRADNJA GLIKOGENA
Glycogenolysis
H2O
The Fate of Glucose-6-phosphate
Glucose-1- P Glucose-6- Pphosphoglucomutase
H2O
Pi glucose Blood
Glycolysis inmuscles forenergy
LIVER
MUSCLES
(glucose-6-phosphatase)
Summary of GlycogenMetabolism
*
* Glucose-6-phosphatase
(immediately after a meal)
(2 to 3 hours following a meal)
No glucose-6-phosphatase
Glucagon binds to receptors in the LIVER ONLY.
Epinephrine binds to receptors primarily in theMUSCLES but also in LIVER.
GTP displacesGDP on the subunit of the Gprotein
GTP on the subunit ishydrolyzed