8/12/2019 Uct Tipos de Columna
1/80
Green Earth
page 4
2012
S O L I D P H A S E E X T R A C T I O N
P R O D U C T S C A T A L O GU C T
800.541.0559 www.unitedchem.com
F O R E N S I C S
8/12/2019 Uct Tipos de Columna
2/80
2
www.unitedchem
.com
F O R E N S I C S C L I N I C A L V E T E R I N A R Y P H A R M A C E U T I C A L
UCT is a respected leader in the drug testing,pharmaceutical, clinical, environmental and
agricultural industries. Our wide range of
highly reproducible solid phase extraction
columns allow the chromatographer a
consistent extraction technique, and our
expertise in silane manufacturing allows a
greater control of the chemical processes
involved in producing our high quality
bonded phase. We manufacture our complete
product line of bonded phases. We manufacture
our complete product line of bonded silicasorbents, packaged in a variety of formats,
including SPE columns, 96 & 48 well plates,
universal cartridges and micro centrifuge tubes.
We also offer a variety of SPE accessories
including derivatizing reagents, GC liners,
and manifolds.
Since our beginnings in 1986, UCT has
evolved into a major competitor in the
field of silica based solid phase extraction
technology. Our commitment to ensuring
the satisfaction of our customers is accom-
plished by delivering on our promises:
top-quality, dependable solid phase
extraction products, and unmatched
technical support.
F O R E N S I C S
8/12/2019 Uct Tipos de Columna
3/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CUSTOMER SERVICE
PRICES AND TERMSOur prices are subject to change without notice. The price
in effect when we receive your order will apply. All prices
are in US Dollars and are F.O.B. Lewistown, PA 17044.
Terms of payment are net 30 days.
MINIMUM ORDERS
We welcome all orders, therefore, we do not have a mini-
mum order requirement. When ordering, please include
your purchase order number, complete Ship To and
Bill To address, catalog number, quantity, and descrip-
tion of product(s). Also include your name and a phone
number where you can be reached should we have anyquestions concerning your order.
Custom items will be evaluated on an individual basis; quantity
requirements may be necessary.
SHIPMENTS
Normal processing is within 24-48 hours after receipt
of an order. Unless special shipping requests have
been made, our trained staff will send all orders by
UPS Ground service. The appropriate shipping charges
(freight & insurance costs) will be added to the invoice,
unless otherwise instructed by the customer.
SPECIAL PRICING
We offer special pricing for volume purchases and stand-
ing orders. Please call a sales representative for more
information on special pricing qualifications.
RETURN POLICYOur Quality Manager will handle all returns. Before return-
ing merchandise, please call to obtain a return authorization
number from your sales representative. We will need
to know the reason for the return, date of purchase,
purchase order number and invoice number in order
to issue a return authorization number. Returned mer-
chandise must be received before a credit can be issued.
Returns will not be accepted after 90 days. A restocking
fee of 25% of the price paid, or a minimum of $25.00
(whichever is greater) will be charged on all returns.
WARRANTYAll products manufactured by UCT are guaranteed against
defects in materials and workmanship for a period of 90
days after shipment. UCT will replace any items that
prove to be defective during this time period.
The exclusive remedy requires the end user to first ad-
vise UCT of the defective product by phone or in writing.
Secondly, the defective product must be returned within
30 days after proper approval from our Quality Manager.
All returns must indicate the purchase order number, the
lot number and the shipping date. UCTs total liability is
limited to the replacement cost of UCT products.
This warranty does not apply to damage resulting
from misuse.
Placing An Order
Phone: 215.781.9255 800.541.0559Fax: 215.785.1226
UCT, Inc.2731 Bartram Rd.Bristol, PA 19007
Technical Support
Phone: 215.781.9255 800.385.3153Fax: 215.785.1226Email: [email protected]: forums.unitedchem.comWeb: www.unitedchem.com
8/12/2019 Uct Tipos de Columna
4/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
4
www.unitedchem
.com
A G R E E N E R E A R T H
Here at UCT, Inc. we are making an effort to keep the planet cleaner
and greener for everyone. It is our belief that we must act now topreserve our environment for future generations to come.
Organizations we support:
Arbor Day Foundation
Audubon Society
Sierra Club
8/12/2019 Uct Tipos de Columna
5/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
TABLE OF CONTENTS
Solid Phase Extraction Phases .....................................................................................................................................................6-8
Reservoirs for Bonded Phase Extractions ......................................................................................................................................9
Use of Bonded Phases for Sample Preparation ...........................................................................................................................10
CLEAN SCREEN Sample Prep Products .................................................................................................................................11-19
Mechanism of CLEAN SCREEN ....................................................................................................................................12
Copolymeric Bonded Phases for Drug Abuse Testing .................................................................................................13
CLEAN SCREEN XCELTM ............................................................................................................................................14-15
CLEAN SCREEN FAStTM ...................................................................................................................................................16
CLEAN SCREENETG / BNZ and CLEAN-THRUTIPS.................................................................................................17
CLEAN SCREEN RSV ................................................................................................................................................18-20
XtrackT HIGH-FLOW Bonded Phases .....................................................................................................................................21-22
CLEAN-UP
Solid Phase Extraction Phases.............................................................................................................................23-46 Hydrophobic Extraction Columns .............. ................ ............... ................ ............... ............... ................ ............... ...24-27
Hydrophilic Extraction Columns .............. ............... ................ ............... ............... ................ ............... ................ .....28-32
Ion Exchange Extraction Columns ................ ............... ................ ............... ................ ............... ............... ................ 33-40
A Map to Optimizing Sample Clean Up using Solid Phase Extraction ..................................................................36-37
Copolymeric Extraction Columns ................ ............... ................ ............... ................ ............... ............... ................ 41-45
Covalent Extraction Columns .............. ............... ............... ................ ............... ................ ............... ................ ............... 46
STYRE SCREEN Polymeric Resin Extraction Columns .........................................................................................................47-48
Benzenesulfonic Acid + C18, Polystyrene Divinylbenzene, Ion Exchange,
Reverse Phase, Carboxylic Acid and Quaternary Amine .............................................................................................48
Method Development Kits ........................................................................................................................................................49-50
Non-Polar Phases Endcapped, Polar Phases, Copolymeric Phases,
Ion Exchange Phases, Toxicology Phases and Pharmaceutical Phases .................................................................... 50
SELECTRASORBTM Bulk Sorbents ............................................................................................................................................51-53
Reservoirs...................................................................................................................................................................................54-55
Frits ..................................................................................................................................................................................................56
Solid Phase Extraction Accessories ..............................................................................................................................................57
Positive Pressure Manifold ......................................................................................................................................................58-59
Universal Vacuum Manifold and Accessories ........................................................................................................................60-61
Vacuum Manifold and Accessories ......................................................................................................................................... 62-63
SELECTRA-SIL Derivatizing Reagents ....................................................................................................................................64-68
GC Liners ....................................................................................................................................................................................69-70
SELECTRA HPLC Columns ...........................................................................................................................................................71
96 Deep Well Plates ...................................................................................................................................................................72-74
48 Deep Well Plates ...................................................................................................................................................................75-77
Flash Chromatography Columns .............................................................................................................................................78-79
8/12/2019 Uct Tipos de Columna
6/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
6
www.unitedchem
.com
SOLID PHASE EXTRACTION PHASES
8/12/2019 Uct Tipos de Columna
7/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
REVERSE PHASE (HYDROPHOBIC)
SORBENT SORBENT CODE STRUCTUREC2 ethyl C02 -SiCH
2CH
3
C3 propyl C03 -Si-(CH2)
2CH
3
C4 n-butyl Cn4 -Si-(CH2)
3CH
3
Ci4 isobutyl Ci4 -Si-CH2CH(CH
3)
2
Ct4 tertiary butyl Ct4 -Si-C(CH3
)3C5 pentyl C05 -Si-(CH
2)
4CH
3
C6 hexyl C06 -Si-(CH2)
5CH
3
C7 heptyl C07 -Si-(CH2)
6CH
3
C8 octyl C08 -Si-(CH2)
7CH
3
C10 decyl C10 -Si-(CH2)
9CH
3
C12 dodecyl C12 -Si-(CH2)
11CH
3
C18 octadecyl C18 -Si-(CH2)
17CH
3
C20 eicosyl C20 -Si-(CH2)
19CH
3
C30 tricontyl C30 -Si-(CH2)
29CH
3
Cyclohexyl CYH1 -SiPhenyl PHY1 -Si
NORMAL PHASE (HYDROPHILIC)
Silica SIL1 -SiOHDiol DOL1 -Si-(CH
2)
3OCH
2CHOHCH
2OH
Cyanopropyl CNP1 -Si-(CH2)
3CN
Florisil FLSAlumina, Acidic ALAAlumina, Neutral ALNAlumina, Basic ALBCarbon CARB
ION EXCHANGE
Anion pkaAminopropyl (1 amine) NAX1 -Si-(CH
2)
3NH
2 9.8
N-2 Aminoethyl (1 & 2 amine) PSA1 -Si-(CH2)
3NH(CH
2)
2NH
2 10.1, 10.9
Diethylamino (3 amine) DAX1 -Si-(CH2)
3N(CH
2CH
3)
2 10.6
Quaternary Amine Chloride QAX1 -Si-(CH2)
3N+(CH
3)
3 Cl- always charged
Quaternary Amine Hydroxide CHQAX1 -Si-(CH2)
3N+(CH
3)
3 OH always charged
Quaternary Amine Acetate CAQAX1 -Si-(CH2)
3N+(CH
3)
3 CH
3COO always charged
Quaternary Amine Formate CFQAX1 -Si-(CH2)
3N+(CH
3)
3 HCOO always charged
Polyimine PAX -Si-(CH2)
3-R-[NHCH
2CH
2]
X
CationCarboxylic Acid CCX1 -Si-CH
2COOH 4.8
Propylsulfonic Acid PCX1 -Si-(CH2)
3SO
3H
8/12/2019 Uct Tipos de Columna
8/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
8
www.unitedchem
.com
FUNCTIONALIZED SILICA-BASED PHASES
REVERSE PHASE (HYDROPHOBIC)
SORBENT % ORGANIC EXCHANGE (meq/g)C2 ethyl 6.60 N/AC3 propyl 7.60 N/AC4 n-butyl 8.50 N/ACi4 isobutyl 8.80 N/ACt4 tertiary butyl 8.50 N/AC5 pentyl 9.50 N/AC6 hexyl 11.00 N/AC7 heptyl 11.00 N/AC8 octyl 11.10 N/AC10 decyl 15.70 N/AC12 dodecyl not tested N/AC18 octadecyl 21.70 N/AC20 eicosyl 24.30 N/AC30 tricontyl 26.00 N/ACyclohexyl 11.60 N/APhenyl 11.00 N/A
NORMAL PHASE (HYDROPHILIC)
Silica N/A N/ADiol 8.00 N/A
Cyanopropyl 6.90 N/AFlorisil N/A N/AAlumina, Acidic N/A N/AAlumina, Neutral N/A N/AAlumina, Basic N/A N/ACarbon N/A N/A
ION EXCHANGE
AnionAminopropyl (1 amine) 6.65 0.310N-2 Aminoethyl (1 & 2 amine) 9.70 0.320Diethylamino (3 amine) 8.40 0.280Quaternary Amine Chloride 8.40 0.250Quaternary Amine Hydroxide 8.40 0.250
Quaternary Amine Acetate 8.40 0.250Quaternary Amine Formate 8.40 0.250Polyimine 13.5 0.250
CationCarboxylic Acid 9.10 0.170Propylsulfonic Acid 7.10 0.180Benzenesulfonic Acid 11.00 0.320Benzenesulfonic Acid High Load 15.00 0.650Triacetic Acid 7.61 Anion 0.17 / Cation .06
COPOLYMERIC (MULTIFUNCTIONAL PHASES)
Aminopropyl + C8 12.3 0.163Quaternary Amine + C8 13.60 0.160Carboxylic Acid + C8 1 2.50 0.105Propylsulfonic Acid + C8 14.62 0.114Benzenesulfonic Acid + C8 12.30 0.072Cyanopropyl + C8 14.60 0.163Cyclohexyl + C8 N/A N/A
COVALENT PHASES
Epoxy N/A N/AAldehyde N/A N/AIsocyanate 7.1 N/AThiopropyl 6.50 N/A
8/12/2019 Uct Tipos de Columna
9/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
Chemistries are offered on these particles sizes...
Small Particle (5-20 m)
Intermediate Particle (25-40 m)
Standard Particle (40-60 m)*
Large Particle (125-210 m)
and are available in the following formats
Stated Volume (mL) Tube Configuration Bed Diameter (mm) Sorbent Mass (mg)
1 Cylindrical 5.5 50-200
3 Cylindrical 8.5 50-1000
6 Cylindrical 12.5 200-200010 Expanded 8.5 50-1000
15 Cylindrical 15.5 500-2000
25 Cylindrical 20 500-5000
75 Cylindrical 27.5 1000-10000
150 Cylindrical 38.0 10000-70000
RESERVOIRS FOR BONDED PHASE EXTRACTIONS
8/12/2019 Uct Tipos de Columna
10/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
10
www.unitedchem
.com
USE OF BONDED PHASES FOR SAMPLE P REPARATION
CONDITIONING, SOLVATION (WETTING)
Columns are shipped dry, but those with hydrophobiccharacter need to be solvated in order to interact efficientlyand reproducibly with aqueous matrices. Sample capacity isseverely reduced on a dry column.
At low vacuum ( - 3 in. Hg) add 1.5 mL of methanol or aceto-nitrile per 100 mg of sorbent to the sample preparation col-umn. Release the vacuum or begin flushing immediately uponcompletion. The more air which passes through the columnbefore sample loading, the less solvated the sorbent will be.
Apply deionized or distilled water to remove excess solventwhich will interfere with hydrophobic binding. Use 1 mL H2Oper 100 mg sorbent. Momentary high vacuum (5 to 8 in. Hg)may be necessary to restart flow. At - 2.5 in. Hg the column
will resist air displacement (vacuum may be left on withoutdrying the sorbent). If the sorbent is accidentally dried, resol-vate and reflush.
When using ion exchange columns, apply 1mL of buffer tothe column after flushing to ensure that the sorbent pH isoptimal for the sorbent analyte interaction desired. Whereion exchange interactions are involved, follow guidelinesconcerning pKa, pH and ionic binding. Use the same vacuumguidelines as described for flushing.
When doing work where a very clean baseline is requiredwashing the column with the elution solvent as part of theconditioning step will improve the LOD and LOQ.
SAMPLE PREPARATION AND APPLICATION
Mechanisms may be hydrophobic, polar, or ionic. Add internalstandard to the sample if quantitation is desired. Optimizesample application by removing particulates if necessary(centrifugation or filtering) and/or diluting viscous matriceswith water or buffer to ensure proper pH for desired inter-actions. The analyte and sorbent should be uncharged foroptimum hydrophobic retention. On ion exchange sorbents,analytes must be oppositely charged to the sorbent [anions(-) on anion exchange sorbents (+); cations (+) on cation ex-change sorbents (-)]. During sample application, the analytebinds by displacing a counterion on the sorbent.
Apply sample at a rate of 1mL/min. Again, a momentary
increase in vacuum may be needed to initiatesample flow.
WASHING THE SORBENT AND ELUTING
Ideal washing removes as many interferences as possiblewhile retaining the analyte(s). Ideal elution recovers 100% ofthe analyte while leaving behind interferences. Make certainyour column is dry when changing between aqueous solu-tions and organic solvents.
HYDROPHOBIC AND POLAR ANALYTES
The best approach towards using these types of sorbents isto search for a solvent mixture which will wash the most inter-ferences from the sorbent without loss of analyte. Note thatwash pH may greatly affect cleanup and/or recovery. Keepanalyte and sorbent pKa in mind if applicable. Elute with thestrongest organic solvent, or by raising the percentage oforganic, possibly in combination with a pH change to disrupt
binding.
ION EXCHANGE
Ionic bonds are strong enough to allow the analyte to remainbound while interferences are washed away with high per-centages (up to 100%) of polar or nonpolar organic solvents.The pH will also affect sample cleanup. Remember to remain2 pH units from the relevant pKa of your analyte and sorbent,both of which need to remain charged for ionic retention.Elute with aqueous buffers containing a stronger counterionthan your analyte (classic ion exchange) or by changing pH todisrupt the ionic attraction. Make sure the elution solvent hasenough organic character to overcome any adsorption due to
the packing material.
COPOLYMERIC EXCHANGE
For ionically bound analytes, use washes of high organicstrength to remove interferences retained by hydrophobic(solvent strength dependent) interactions. If your analyte isalso capable of hydrophobic binding, remove polar interfer-ences ionically similar to your analyte by using aqueous orweak aqueous/organic washes while disrupting ionic (pH andionic strength dependent) binding. Elute by simultaneouslydisrupting ionic and hydrophobic interactions
8/12/2019 Uct Tipos de Columna
11/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN SCREEN SAMPLE PREP PRODUCTS
8/12/2019 Uct Tipos de Columna
12/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
12
www.unitedchem
.com
MECHANISM OF CLEAN SCREEN
Sample ApplicationWhen a sample is loaded onto the column at pH 6, many carboxylicacid functionalities present in the sample are ionized. This creates arepulsion between the column and many sample borne interferences,thereby reducing the likelihood of their adsorbing onto the column.At this pH, ibuprofen & barbiturates are not i onized and arehydrophobically adsorbed on to the column (figure 1). At the sametime, drugs with amine functionalities such as cocaine andphencyclidine adsorb on to the column via both hydrophobic andionic attraction (figure 1).
The column can then be washed with water or weak aqueousbuffers at or below pH 6 without risking loss of the analytes. Afterdrying the column, it is possible to elute the hydrophobically boundanalytes using solvents of minimal polarity such as methylenechloride or a hexane/ethyl acetate mixture (figure 2). Cationic
analytes will remain bound to the column. Many compounds ofintermediate polarity and potential interferences will also remainon the column. The majority of these potential interferences canbe removed by using a methanol wash.
Cationic analytes bound to the column can be eluted after anotherdrying step. The drying steps are necessary to remove water whichwould have prevented the water immiscible elution solvents fromoptimally interacting with the analytes (figure 3).
To elute the cationic analytes, an organic solution with a high pHshould be used. A methylene chlorideisopropanol-ammoniumhydroxide mixture will simultaneously disrupt these ionic
interactions and successfully elute the desired compound (figure 4).
NH+
PCP
SO3
CnH2n+1
NH+
PCPSO3
CnH2n+1
H2O
NH+
PCP
IBUPROFEN
SO3
CnH2n+1
H
CH3 O
figure
Dry Column
Elution 2
NH+
PCP
IBUPROFEN
SO3
CnH2n+1
CH C OH
CH3 O
Elution 1
figure 2
figure 3
figure 4
CH C O
8/12/2019 Uct Tipos de Columna
13/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN SCREEN
COPOLYMERIC BONDED PHASES FOR DRUG ABUSE TESTIN
Analytical demand for more efficient, robust and clean extraction of drugs from biological matrices led to thedevelopment of CLEAN SCREENsorbents. Since 1986, CLEAN SCREENhas led the industry with dependableand reproducible Solid Phase Extraction products and applications. CLEAN SCREENphases are true copolymericsorbents that contain hydrophobic and ion exchange functional groups uniquely polymerized to a silica substrate.The design and quality of CLEAN SCREENprovides superior sample clean up, recovery and reproducibility.
Mixed mode separations allow maximum selectivity for extraction of acids, neutrals and bases. This selectivitymakes CLEAN SCREENideal for both screening and confirmation analysis for virtually all drug categories.CLEAN SCREENcolumns are used extensively by forensic and clinical chemists including:
Note:If performing extractions out of viscous matrices such as tissue or horse urine, turn to our XtrackT section,where high-flow/gravity flow columns are found. The DAU CLEAN SCREEN sorbent as well as other phasesare available in this larger particle size.
Post Mortem Investigations Criminal Investigations Urine Drug Testing Athletic Drug Testing
Racing Laboratories Therapeutic Drug Monitoring Medical Drug Screening
CLEAN SCREENDAU
column is copolymerized on arigid, purified silica gel support.The two functional groupsinclude a reverse phase, and anion exchanger, benzenesulfonicacid. This column is commonlyused for analyzing a wide rangeof drugs of abuse, includingacidic, basic & neutral drugs.
Application:Dual functionalityfor weak bases and hydrophobiccompounds.
CLEAN SCREENTHC
column is copolymerized on arigid, purified silica gel support.The two functional groups includea reverse phase, and an ionexchanger, primary amine.This column is commonlyusedfor analyzing THC andits metabolites.
Application:Dual functionality for
acids and hydrophobic compounds.
DAU
Part Number without Part Number with Sorbent Amount/ Unit per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
CSDAU131 CCDAU131 130mg/1mL 100CSDAU133 CCDAU133 130mg/3mL 50CSDAU203 CCDAU203 200mg/3mL 50CSDAU303 CCDAU303 300mg/3mL 50CSDAU503 CCDAU503 500mg/3mL 50CSDAU206 CCDAU206 200mg/6mL 50CSDAU506 CCDAU506 500mg/6mL 50CSDAU1M6 CCDAU1M6 1g/6mL 30ZSDAU005 ZCDAU005 50mg/10mL 50ZSDAU013 ZCDAU013 130mg/10mL 50ZSDAU020 ZCDAU020 200mg/10mL 50CSDAU515 CCDAU515 500mg/15mL 5
% Organic Loading: 12.30 Exchange Capacity (meq/g): 0.072
THC
Part Number without Part Number with Sorbent Amount/ Unit per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
CSTHC131 CCTHC131 130mg/1mL 100CSTHC203 CCTHC203 200mg/3mL 50CSTHC303 CCTHC303 300mg/3mL 50CSTHC503 CCTHC503 500mg/3mL 50CSTHC206 CCTHC206 200mg/6mL 50CSTHC506 CCTHC506 500mg/6mL 50CSTHC1M6 CCTHC1M6 1g/6mL 30ZSTHC013 ZCTHC013 130mg/10mL 50ZSTHC020 ZCTHC020 200mg/10mL 50
% Organic Loading:12.3 Exchange Capacity (meq/g):0.163
GHB
Part Number without Part Number with Unit per
Clean-ThruTips Clean-ThruTips Pack
CSGHB203 200mg/3mL 50ZSGHB020 200mg/10mL 50
The small polar nature of the molecule and the lack of a UV chromatophore complicate the chromatographic andspectrophotometricanalysis of GHB. Chemically, GHB is unstable and readily forms Gamma-butyrolactone when heated inacid conditions. Most analytical methods are based upon the interconversion to the lactone and chemical derivatization to
form the TMS derivative. This column is for the extraction of free GHB.
8/12/2019 Uct Tipos de Columna
14/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
14
www.unitedchem
.com
CLEAN SCREEN XCELTM 2 STEP EXTRACTION COLUMN
8/12/2019 Uct Tipos de Columna
15/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN SCREEN XCEL
SOLID PHASE EXTRACTION COLUMNS
CLEAN SCREEN XCEL I
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CSXCE111 130mg/1mL 100CSXCE103 130mg/3mL 50CSXCE106 130mg/6mL 50ZSXCE010 130mg/10mL 50
CLEAN SCREEN XCEL II
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CSXCE211 130mg/1mL 100CSXCE2103 130mg/3mL 50CSXCE2106 130mg/6mL 50ZSXCE2010 130mg/10mL 50
CLEAN SCREEN XCEL
SOLID PHASE EXTRACTION WELL PLATES
96 DEEP WELL PLATES
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
WSH96XCE11 130mg 1
48 DEEP WELL PLATES
Part Number Sorbent Amount/ Unit per Tube Volume Pack
WSH48XCE11 130mg 1
CLEAN SCREEN XCEL I:
The Xcel I column will extract awide array of basic drugs includingbenzodiazepines and opiates.
CLEAN SCREEN XCEL II:
The Xcel II column is designed solely forthe extraction of the THC metabolite.
The Clean Screen Xcel solid phase extraction columns are a line of sample prep phases designedto reduce the number of steps in the extraction, and therefore the amount of time and solventnecessary to complete the sample cleanup. Further advantages include reduced sample size andimproved cleanliness and recovery.
8/12/2019 Uct Tipos de Columna
16/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
16
www.unitedchem
.com
CLEAN SCREEN FASt
SOLID PHASE EXTRACTION COLUMNS
CLEAN SCREEN FAStTM
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CSFAS203 200mg/3mL 50
ZSFAS020 200mg/10mL 50
Benefits: Eliminate the centrifuge and sample transfer steps Lower costs by decreasing turn-around time Reducing instrument and LC column maintenance
CLEAN SCREEN FAStTMemploys a process that uses positive pressure and a solid phase sorbent bed builtwith small pore frits to quickly and efficiently prepare samples for LC/MS analysis. This method eliminatesthe timely centrifugation, reduces matrix suppression effects and removes particulates greater than ~ 1m.Samples can be diluted at a ratio as low as 1:1, which is `useful for analytes at very low concentrations.
A FASTER AND CLEANER SPE
ALTERNATIVE TO
DILUTE AND SHOOT
8/12/2019 Uct Tipos de Columna
17/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-THRU TIPS
CLEAN SCREEN
ETHYL GLUCURONIDE SOLID PHASE EXTRACTION COLUMN
BENZODIAZEPINE SOLID PHASE EXTRACTION COLUMN
ETG
Part Number without Part Number with Sorbent Amount/ Unit per
Clean-Thru
Tips Clean-Thru
Tips Tube Volume PackCSETG203 CCETG203 200mg/3mL 50ZSETG040 ZCETG040 400mg/10mL 50
BNZ
Part Number without Part Number with Sorbent Amount/ Unit per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
CSBNZ203 CCBNZ203 200mg/3mL 50CSBNZ206 _______ 200mg/6mL 50ZSBNZ020 _______ 200mg/10mL 50ZSBNZ030 _______ 300mg/10mL 50
CLEAN-THRUTIPS
Part Number Description Unit per bag
CLTTP050 TIP 50
CLEAN SCREENETG
columns are available exclusivelyfrom UCT for analysis by GC/MS &LC/MS. This 3mL column contains aproprietary carbon packingmaterial for the extraction andconcentration of ethyl glucuronide.
CLEAN SCREENBNZ
columns are available forbenzodiazepine extractions,with specific focus on7-amino benzodiazepines.
CLEAN-THRU tips provides the first solid phase extraction cartridge system that eliminates sample carry overfrom the vacuum manifold lid. The technology was pioneered by our research scientists & consists of one of ourSPE columns with a disposable tip that attaches to the end of each column. Columns available in the CLEAN-THRU configuration are found in the CLEAN SCREENsections. This system was designed in order to meet the strictrequirements that the Substance Abuse and Mental Health Services Administration certification has placed on labo-ratories to address the problem of cross contamination between samples. CLEANTHRUtips provide a completely
disposable system that eliminates any contact between the sample, wash solvents and the extraction apparatus.The continuous passage of the sample through the system provides a direct, accurate route to waste or collectionvessels. As each extraction is completed, the column and tip are discarded as a unit. CLEAN-THRUeliminates yourconcerns about sample residue remaining in the extraction system.U CLEAN SCREENsorbent as well as otherphases are available in this larger particle size.
StandardConfiguration
UCTs Column with
a CLEAN-THRUTip
8/12/2019 Uct Tipos de Columna
18/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
18
www.unitedchem
.com
Advantages of CLEAN SCREENRSV:
75% Reduction in total liquid volumes
Lower cost per extraction
Faster extraction times
Less disposal cost
Increased automated throughput
50% Reduction in eluate volume
Faster dry down times
Reduced exposure to organic solvents
Superior flow characteristics
Less flow restriction from matrix proteins
or particulates
More reliable for automated processing
High capacity
Greater linear range
CLEAN SCREENRSV REDUCED SOLVENT VOLUME EXTRACTION COLUMNS
Reduced Solvent Volume extraction columns are micro bed packed columns whichoffer the advantages of disc technology yet maintain the proven track record of ourconventional SPE columns. Reduced Solvent Volume columns use 75% less solvent thantraditional packed columns. Less solvent means faster extractions, higher throughputand less waste disposal, which translates into significant savings in both time andmoney. Results demonstrate that therapeutic and abused drugs in urine and bloodmatrices can be extracted with cleanliness, high recoveries and consistent reproducibilityby using the Reduced Solvent Volume extraction column.
8/12/2019 Uct Tipos de Columna
19/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CAPACITY
SOLVENT REDUCTION
RECOVERY
CLEAN SCREEN RSV
REDUCED SOLVENT VOLUME EXTRACTION COLUMNS
91% (n=4) for 1000 ng/mL90% (n=2) for 3000 ng/mL
91% (n=4) for 4000 ng/mL
No analyte breakthrough at 1000 ng/mL
and less than 0.2% analyte breakthrough
at 3000 and 4000 ng/mL levels.
Linear Ranges Exhibiting
Greater than
85% Recoveries in Urine
THC-COOH Standards (ng/mL) in spiked urine
Analyte
AbsoluteRecovery
Concentration(ng/mL)
100908070605040302010
0
200018001600140012001000
800600400200
0
1000 3000 4000
Highest Concentration Tested
Lowest Concentration Tested
Analyte Solvent Usage Solvent Usage % Solvent
Clean-ThruTips Reduced Solvent Traditional Packed Reduction
Volume SPE columns columns
Barbiturates 4.25 mL 18 mL 76%Benzoylecgonine 4.65 mL 19 mL 76%THC-COOH 4.85 mL 16.4 mL 73%Phencyclidine 5.15 mL 19 mL 70%
Butalbital
Amobarbital
Pentobarbital
Secobarbital
Phenobarbital
Benzoylecgonine
Phencyclidine
THC-COO
H
2ng/mL25ng/mL
8/12/2019 Uct Tipos de Columna
20/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
20
www.unitedchem
.com
CLEAN UP RSV
REDUCED SOLVENT VOLUME EXTRACTION COLUMNS
CLEAN SCREEN RSV
REDUCED SOLVENT VOLUME EXTRACTION COLUMNS
COPOLYMERIC EXTRACTION FOR ALL DRUG CLASSES
Part Number without Part Number with Sorbent Amount/ Unit per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
CSDAUA83 CCDAUA83 80mg/3mL 50ZSDAUA08 ZCDAUA08 80mg/10mL 50
SPECIFICALLY FOR THC & THCA EXTRACTION
Part Number without Part Number with Sorbent Amount/ Unit per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
CSTHCA83 CCTHCA83 80mg/3mL 50ZSTHCA08 ZCTHCA08 80mg/10mL 50
C18, OCTADECYL TRI-FUNCTIONAL
Part Number Part Number Sorbent Amount/ Unit per Pack
Endcapped Unendcapped Tube Volume Pack
CEC18A083 CUC18A083 80mg/3mL 50
CLEAN SCREENDAU
Column is copolymerized on a rigid,
purified silica gel support. The twofunctional groups include a reverse phaseand an ion exchanger, benzenesulfonicacid. This column is commonly used foranalyzing a wide range of drugs of abuse,including acidic, basic and neutral drugs.
CLEAN SCREENTHC
Column is copolymerized on a rigid,purified silica gel support. The twofunctional groups include a reversephase, and an ion exchanger, quaternary
amine. This column is commonly usedfor analyzing THC and its metabolites.
% Organic Loading:21.70Application:
Removes hydrophobic impurities,de-salting and purificationof hydrophobic compounds.
8/12/2019 Uct Tipos de Columna
21/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
XtrackT HIGH-FLOW SOLID PHASE EXTRACTION COLUMNS
8/12/2019 Uct Tipos de Columna
22/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
22
www.unitedchem
.com
XtrackT
HIGH-FLOW BONDED PHASES
Viscous sample matrices are frequently resistant to flow through standard solid phase columns. Increased particlesize enhances flow characteristics allowing ease of sample application and analysis. XtrackTcolumns are designedto give uniform flow for even the most viscous samples including equine urine, post mortem blood and tissues,meconium, amniotic fluid, milk, etc.
XtrackTalso functions as a gravity flow column for most blood and urine samples. A single column providesextraction for a broad spectrum of compounds with selective elution of acid neutrals, steroids and bases XtrackTyields very clean extractions and excellent recoveries without need for additional liquid clean up steps. XtrackT is available in hydrophobic, hydrophilic, ion exchange, and copolymeric phases, including the CLEAN SCREENDAU sorbent. XtrackTis recommended for any chemist challenged by viscous sample matrices, or those desiringgravity flow capacity.
Upon request, we can also provide any of our CLEAN-UPsorbents in this large particle size.
Advantages of XtrackT
Resists plugging Improved flow of all sample types Gravity flow of most samples
Broad spectrum extractions Very clean extractions High recoveries Reproducibility
GRAVITY-FLOW CLEAN SCREENDAU COLUMNS
Part Number with Part Number without Sorbent Amount/ Unit per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
XRDAH203 XCDAH203 200mg/3mL 50XRDAH303 XCDAH303 300mg/3mL 50XRDAH503 XCDAH503 500mg/3mL 50XRDAH206 XCDAH206 200g/6mL 50XRDAH506 XCDAH506 500g/6mL 50XRDAH20Z XCDAH20Z 200mg/10mL 50XRDAH50Z XCDAH50Z 500mg/10mL 50XRDAH515 XCDAH515 500mg/15mL 50XRDAHM15 XCDAHM15 1g/15mL 30
CLEAN SCREENDAU
Column is copolymerized on a rigid,purified silica gel support. The twofunctional groups include a reversephase, and an ion exchanger ,benzenesulfonic acid. This column iscommonly used for analyzing a widerange of drugs of abuse, includingacidic, basic & neutral drugs.
HIGH-FLOW CLEAN-UPENDCAPPED C18 COLUMNS
Part Number with Part Number without Sorbent Amount/ Unit
per
Clean-ThruTips Clean-ThruTips Tube Volume Pack
XRODHM06 XCODHM06 1g/6mL 30XRODH515 XCODH515 500mg/15mL 50
% Organic Loading: 21.70
Application:
Removes hydrophobic impurities,de-salting and purification ofhydrophobic compounds.
HIGH-FLOW CLEAN-UPPROPYLSULFONIC ACID COLUMNS
Part Number with Part Number without Sorbent Amount/ Unit perClean-ThruTips Clean-ThruTips Tube Volume Pack
XRPCH50Z XCPCH50Z 500mg/10mL 50
% Organic Loading: 9.70
Application:
Scavenger for amines,alcohols and other nucleophiles
High-Flow also available in:
Carboxylic Acid, Aminoethyl, Benzenesulfonic Acid and Quaternary Amine
8/12/2019 Uct Tipos de Columna
23/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
HYDROPHOBIC HYDROPHILIC ION EXCHANGE
CLEAN-UP SOLID PHASE EXTRACTION COLUMNS
8/12/2019 Uct Tipos de Columna
24/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
24
www.unitedchem
.com
CLEAN-UP
HYDROPHOBIC EXTRACTION COLUMNS
This sorbent is composed of a silica backbone bonded
with hydrocarbon chains. It is used to extract compounds
which exhibit non-polar or neutral characteristics out of
complex matrices. The C18 phase is the most widely used
for non-polar interactions because of its nonselective nature;
C18 will extract a large number of compounds with differing
chemical properties. To enchance selectivity, UCT offers a
wide range of hydrophobic sorbents. Multiple chain configu-
rations are available for some Endcapped or unendcapped
sorbents are available for all chain lengths.
Analytes* Washes Elutions
alkanes aqueous, non-polar
alkenes usually with to
aromatics some polar polar organic
neutral compounds organic solvent
*typical compounds which can be extracted using hydrophobic columns
Silica Backbone
Hydrocarbon Chain
O
Si
O
Si
O
Si
O
Si
O Si (CH2)17 CH3
CH3(CH2)SiO
OSi(CH3
)3
OSi(CH3)3
OSi(CH3)3
OSi(CH3)3
17
Example of a Hydrophobic Phase
8/12/2019 Uct Tipos de Columna
25/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
HYDROPHOBIC EXTRACTION COLUMNS
MECHANISM OF HYDROPHOBIC BONDING
Compounds are retained by non-polar interactionsfrom polar solvents or matrix environments. They are
bound by dispersion forces / van der Waals forces.Elution, or disruption of the non-polar interactionsis achieved by solvents or solvent mixtures with suf-ficient non-polar character. Some polar solvents, suchas acetonitrile have enough non-polar characteristicsto disrupt nonpolar binding to cause elution of acompound from the sorbent. Methanol can be usedas well, although it should be noted that it will takeoff both polar and nonpolar analytes of interest andinterferences.
UNENDCAPPED VS. ENDCAPPED
Bonded phases are manufactured by the reactionof organosilanes with activated silica. During thepolymerization reaction of carbon chains to the silicabackbone, a very stable silyl ether linkage forms. Ourunendcapped columns allow hydroxyl sites to remain,thus making these columns slightly hydrophilic.
In order to decrease this slight polarity, thesehydroxyl sites are deactivated. Proprietary bondingtechniques ensure that these sites are 100% reacted,leading to a complete endcapping. Because thereare no hydroxyl sites left, our endcapped columnsare more hydrophobic than our unendcapped columns.
Sorbent
Compound
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
H
C
H
O
O
O
SiSi
Si
OSi(CH3)3
OSi(CH3)3
O
O
O
SiSi
Si
OH
OH
Unendcapped Structure Endcapped Structure
Example of a Hydrophobic Bonding
Sorbent Structure
C2 ethyl -SiCH2CH3
C3 propyl -Si-(CH2)
2CH
3
C4 n-butyl -Si-(CH2)
3CH
3
Ci4 isobutyl -Si-CH2CH(CH
3)
2
Ct4 tertiary butyl -Si-C(CH3)3
C5 pentyl -Si-(CH2)
4CH
3
C6 hexyl -Si-(CH2)
5CH
3
C7 heptyl -Si-(CH2)
6CH
3
C8 octyl -Si-(CH2)
7CH
3
C10 decyl -Si-(CH2)
9CH
3
C12 dodecyl -Si-(CH2)
11CH
3
C18 octadecyl -Si-(CH2)
17CH
3
C20 eicosyl -Si-(CH2)
19CH
3
C30 tricontyl -Si-(CH2)
29CH
3
Cyclohexyl -Si
Phenyl -Si
Hydrophobic Sorbents & Structures
8/12/2019 Uct Tipos de Columna
26/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
26
www.unitedchem
.com
CLEAN-UP
HYDROPHOBIC EXTRACTION COLUMNS
C8, OCTYL
Part Number Part Number Sorbent Amount/ Unit per
Endcapped Unendcapped Tube Volume Pack
CEC081L1 CUC081L1 50mg/1mL 100CEC08111 CUC08111 100mg/1mL 100CEC08113 CUC08113 100mg/3mL 50CEC08123 CUC08123 200mg/3mL 50CEC08153 CUC08153 500mg/3mL 50CEC08156 CUC08156 500mg/6mL 50CEC081M6 CUC081M6 1g/6mL 30CEC0811Z CUC0811Z 100mg/10mL 50CEC0812Z CUC0812Z 200mg/10mL 50CEC0812M15 CUC0812M15 2g/15mL 20CEC0815M25 CUC0815M25 5g/25mL 20
C18, OCTADECYL
Part Number Part Number Sorbent Amount/ Unit per
Endcapped Unendcapped Tube Volume Pack
CEC181L1 CUC181L1 50mg/1ml 100CEC18111 CUC18111 100mg/1ml 100CEC18113 CUC18113 100mg/3ml 50CEC18123 CUC18123 200mg/3ml 50CEC18153 CUC18153 500mg/3ml 50CEC18156 CUC18156 500mg/6ml 50CEC181M6 CUC181M6 1g/6ml 30CEC1811Z CUC1811Z 100mg/10ml 50CEC1812Z CUC1812Z 200mg/10ml 50CEC1815Z CUC1815Z 500mg/10ml 50CEC1812M15 CUC1812M15 2g/15ml 20CEC1815M25 CUC1815M25 5g/25ml 20
CEC18110M375 CUC18110M75 10g/75ml 10
C30, TRICONTYL
Part Number Part Number Sorbent Amount/ Unit per
Endcapped Unendcapped Tube Volume Pack
CEC30111 CUC30111 100mg/1mL 100CEC30123 CUC30123 200mg/3mL 50CEC30156 CUC30156 500mg/6mL 50CEC301M6 CUC301M6 1g/6mL 30
% Organic Loading: 11.10
Application:
Removes large or morehydrophobic compounds.
% Organic Loading: 8.50
Application:
Removes large or morehydrophobic compounds.
% Organic Loading: 26.00
Application:
Removes large or morehydrophobic compounds.
8/12/2019 Uct Tipos de Columna
27/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
HYDROPHOBIC EXTRACTION COLUMNS
CYCLOHEXYL
Part Number Part Number Sorbent Amount/ Unit per
Endcapped Unendcapped Tube Volume Pack
CECYH123 CUCYH123 200mg/3mL 50CECYH153 CUCYH153 500mg/3mL 50CECYH1M6 CUCYH1M6 1g/6mL 30CECYH12M15 CUCYH12M15 2g/15mL 20
PHENYL
Part Number Part Number Sorbent Amount/ Unit per
Endcapped Unendcapped Tube Volume Pack
CEPHY1L1 CUPHY1L1 50mg/1mL 100
CEPHY111 CUPHY111 100mg/1mL 100CEPHY123 CUPHY123 200mg/3mL 50CEPHY153 CUPHY153 500mg/3mL 50CEPHY156 CUPHY156 500mg/6mL 50CEPHY1M6 CUPHY1M6 1g/6mL 30CEPHY12Z CUPHY12Z 200mg/10mL 50
% Organic Loading: 11.60
Application:
Scavenger for phenolic compounds.
% Organic Loading: 11.0
Application:
Scavenger for polar compounds.
OTHER REVERSE PHASES AVAILABLE:
C2 ethyl
C3 propyl
C4 n-butyl
Ci4 isobutyl
Ct4 tertiary butyl
C5 pentyl
C6 hexyl
C7 heptyl
C10 decyl
C12 dodecyl
C20 eicosyl
8/12/2019 Uct Tipos de Columna
28/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
28
www.unitedchem
.com
This sorbent is composed of a
silica backbone bonded with
carbon chains containing polar
functional groups. Groups which
will possess such polarity include
amines, hydroxyls and carbonyls.
Analytes* Washes Elutions
R-OH, R-SH non-polar organic polar organic
R-NH2, solvents solvent
R2-NH, ie: hexane/ethyl acetate (80:20) usually with
R3-N methylene chloride some aqueous
CLEAN-UP HYDROPHILIC NORMAL PHASE EXTRACTION COLUMNS
O
Si
O
Si
OSi
O
Si
O Si (CH2)3 CH2O CH 2CH
OH OH OH
OH
O Si (CH2)3 CH2O CH 2CH
OH OH OH
OH
Silica BackboneHydrophilic Chain
Example of a Hydrophilic Phase
8/12/2019 Uct Tipos de Columna
29/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
Mechanism of
Hydrophilic Bonding
Compounds are retained on hydrophilic
sorbents through polar interactions including
hydrogen bonding, pi-pi or dipole-dipole
interaction. These types of interactions
occur when a distribution of electrons
between individual atoms in functional
groups is unequal, causing negative and
positive polarity. Compounds typically
extracted on a hydrophilic column include
analytes which have polar groups,
including amines, hydroxyls and carbonyls.
Elution is performed by strong polar solvents.
Sorbent Structure
Silica -SiOH
Diol -Si-(CH2)
3OCH
3CHOHCH
2OH
Cyanopropyl -Si-(CH2)
3CN
Note: If un-ionized, ion exchange sorbents can be used as hydrophilic (polar) sorbents.
Hydrophilic
Sorbents & Structures
Example of Hydrophilic Bonding
HO
CH2
OH
CH
HO C CH2
O
CH2Compound
Sorbent
+
+
-
-
CLEAN-UP HYDROPHILIC NORMAL PHASE EXTRACTION COLUMNS
8/12/2019 Uct Tipos de Columna
30/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
30
www.unitedchem
.com
CLEAN-UP
HYDROPHILIC EXTRACTION COLUMNS
UNBONDED SILICA (ACID WASHED)
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUSIL111 100mg/1mL 100CUSIL123 200mg/3mL 50CUSIL153 500mg/3mL 50CUSIL156 500mg/6mL 50CUSIL1M6 1g/6mL 30CUSIL11Z 100mg/10mL 50CUSIL12M15 2g/15mL 20CUSIL15M25 5g/25mL 20CUSIL110M75 10g/75mL 10
PHARMA-SIL
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume PackPHSIL1L1 50mg/1mL 100PHSIL123 200mg/3mL 50PHSIL156 500mg/6mL 50PHSIL1M6 1g/6mL 30PHSIL12M15 2g/15mL 20PHSIL15M25 5g/25mL 20
HIGH SURFACE ACTIVITY SILICA
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
HSSIL153 500mg/3mL 50HSSIL156 500mg/6mL 50
FLORISIL
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUFLS111 100mg/1mL 100CUFLS123 200mg/3mL 50CUFLS153 500mg/3mL 50
CUFLS156 500mg/6mL 50CUFLS1M6 1g/6mL 30CUFLS11Z 100mg/10mL 50CUFLS12Z 200mg/10mL 50CUFLS15Z 500mg/10mL 50CUFLS12M15 2g/15mL 20CUFLS15M25 5g/25mL 20CUFLS110M75 10g/75mL 10
40-63 m Particle Size60A Pore Size
500m2/g Surface Area
Application:
Removes hydrophilic (polar)impurities, purification ofhydrophilic (polar) compounds.
40-63 m Particle Size
60A Pore Size525m2/g Surface Area
Application:
Removes hydrophilic (polar)impurities, purification ofhydrophilic (polar) compounds.
40-63 m Particle Size60A Pore Size547m2/g Surface Area
Application:
Removes hydrophilic (polar)impurities, purification ofhydrophilic (polar) compounds.
% Organic Loading: N/A
Application:
Removes polar-type compounds.
Florisil
products aremanufactured byU.S. Silica, Co.
8/12/2019 Uct Tipos de Columna
31/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
HYDROPHILIC EXTRACTION COLUMNS
ALUMINA, ACIDIC
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUALA111 100mg/1mL 100CUALA123 200mg/3mL 50CUALA153 500mg/3mL 50CUALA156 500mg/6mL 50CUALA1M6 1g/6mL 30CUALA12M15 2g/15mL 20
ALUMINA, BASICPart Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUALB123 200mg/3mL 50CUALB153 500mg/3mL 50CUALB156 500mg/6mL 50CUALB1M6 1g/6mL 30CUALB12Z 200mg/10mL 50CUALB15Z 500mg/10mL 50CUALB12M15 2g/15mL 20CUALB15M25 5g/25mL 20CUALB110M75 10g/75mL 10
ALUMINA, NEUTRAL
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUALN1L1 50mg/1mL 100CUALN111 100mg/1mL 100CUALN123 200mg/3mL 50CUALN153 500mg/3mL 50CUALN156 500mg/6mL 50CUALN1M6 1g/6mL 30CUALN12Z 200mg/10mL 50CUALN15Z 500mg/10mL 50CUALN12M15 2g/15mL 20
CUALN15M25 5g/25mL 20
% Organic Loading: N/A
Application:Removes polar-type compounds.
% Organic Loading: N/A
Application:
Removes polar-type compounds.
% Organic Loading: N/A
Application:
Removes polar-type compounds.
8/12/2019 Uct Tipos de Columna
32/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
32
www.unitedchem
.com
% Organic Loading: 6.90
Application:Removes steroid-type compounds.
% Organic Loading: 8.00
Application:
Removes hydrophilic (polar)impurities, purification ofhydrophilic (polar) compounds.
CLEAN-UP
HYDROPHILIC EXTRACTION COLUMNS
CN, CYANOPROPYL
Part Number Part Number Sorbent Amount/ Unit per
Endcapped Unendcapped Tube Volume Pack
CECNP1L1 CUCNP1L1 50mg/1mL 100CECNP111 CUCNP111 100mg/1mL 100CECNP123 CUCNP123 200mg/3mL 50CECNP153 CUCNP153 500mg/3mL 50CECNP156 CUCNP156 500mg/6mL 50CECNP1M6 CUCNP1M6 1g/6mL 30CECNP12M15 CUCNP12M15 2g/15mL 20CECNP110M75 CUCNP110M75 10g/75mL 10
DIOL
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUDOL111 100mg/1mL 100CUDOL123 200mg/3mL 50CUDOL153 500mg/3mL 50CUDOL156 500mg/6mL 50CUDOL15Z 500mg/10mL 50CUDOL12M15 2g/15mL 20
CARBON-GRAPHITIZED NON-POROUS, 120/400 MESH
Part Number Sorbent Amount/ Unit per
Endcapped Tube Volume Pack
CUCARB1L1 50mg/1mL 100CUCARB111 100mg/1mL 100CUCARB123 200mg/3mL 50CUCARB153 500mg/3mL 50CUCARB156 500mg/6mL 50CUCARB1M6 1g/6mL 30
CLEAN-UP Carbon
Application:
Carbon supports have been used toisolate extremely polar organic com-pounds. They work by a hydrophobicmechanism with a high surface areaand ion exchange. This interaction canhappen in a wide range of polar andnon-polar solvents.
8/12/2019 Uct Tipos de Columna
33/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
UThis sorbent is composed of a silica back-bone bonded with a carbon chain terminatedby a negatively or positively charged functionalgroup. Ion exchange interactions occur be-tween a sorbent that carries a charge anda compound of opposite charge.
This electrostatic interaction is reversibleby neutralizing the sorbent and /or analyte.Ion exchange bonds can also be disruptedby introduction of a counter ion to com-pete with the analyte for binding sites onthe sorbent.
Anions Cations
Organic solvent or aqueous
buffer at pH that allows the ion
to remain charged AND/OR at
a low ionic strength AND/OR
at a weak concentration.
Organic solvent or aqueous
buffer at pH that would neutralize
the ion AND/OR at a high
ionic strength AND/OR at a
strong concentration.
Si
O
Si
O
Si
O Si (CH2)2
OH
OH
SO3
SO3
O
Si
O
O Si (CH2)
OH
2
OH
Silica Backbone
Anion Exchanger
Example of a Cation Exchange Phase
Example of a Anion Exchange Phase
O
Si
O
Si
O
Si
O
Si
O Si (CH2)3 NH+
3
NH +3(CH2)3SiO
OH
OH
OH
OH
Silica Backbone
Anion Exchanger
CLEAN-UP ION EXCHANGE EXTRACTION COLUMNS
)
8/12/2019 Uct Tipos de Columna
34/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
34
www.unitedchem
.com
MECHANISM OF ION EXCHANGE BONDING
Compounds are retained on the sorbent through ionic bonds. Therefore, it is essential that the sorbent and theanalyte to be extracted are charged. Generally, the number of molecules with charged cationic groups increasesat pH values below the molecules pKa value. The number of molecules with charged anionic groups decreases atpH values below the molecules pKa value. To ensure 99% or more ionization, the pH should be at least two pHunits below the pKa of the cation and two pH units above the pKa of the anion. Elution occurs by using a solventto raise the pH above the pKa of the cationic group or to lower the pH below the pKa of the anion to disruptretention. At this point, the sorbent or compound will be neutralized.
ION EXCHANGE SORBENTS & STRUCTURE
Sorbent Structure pKa
Anion Exchangers
Aminopropyl (1 amine) -Si-(CH2)3NH
3+ 9.8
N-2 Aminoethyl (1 & 2 amine) -Si-(CH2)3NH
2+(CH
2)2NH
3+ 10.1, 10.9
Diethylamino (3 amine) -Si-(CH2)3NH+(CH
2CH
3)2 10.6
Quaternary Amine Chloride -Si-(CH2)3N+(CH
3)
3 Cl- always charged
Quaternary Amine Hydroxide -Si-(CH2)3N+(CH
3)
3 OH always charged
Quaternary Amine Acetate -Si-(CH2)3N+(CH
3)
3 CH
3COO always charged
Quaternary Amine Formate -Si-(CH2
)3
N+(CH3
)3
HCOO always chargedPolyimine -Si-(CH
2)3R-[NHCH
2CH
2]
X
Cation Exchangers
Carboxylic Acid -Si-CH2COOH 4.8
Propylsulfonic Acid -Si-(CH2)3SO
3H
8/12/2019 Uct Tipos de Columna
35/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
ANION EXTRACTION COLUMNS
AMINOPROPYL
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUNAX1L1 50mg/1mL 100CUNAX111 100mg/1mL 100CUNAX123 200mg/3mL 50CUNAX153 500mg/3mL 50CUNAX156 500mg/6mL 50CUNAX1M6 1g/6mL 30CUNAX11Z 100mg/10mL 50CUNAX12Z 200mg/10mL 50CUNAX15Z 500mg/10mL 50CUNAX12M15 2g/15mL 20CUNAX15M25 5g/25mL 20CUNAX110M75 10g/75mL 10
PSA ( N-2 AMINOETHYL )
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUPSA1L1 50mg/1mL 100CUPSA111 100mg/1mL 100CUPSA123 200mg/3mL 50CUPSA153 500mg/3mL 50CUPSA156 500mg/6mL 50CUPSA1M6 1g/6mL 30CUPSA11Z 100mg/10mL 50CUPSA110M75 10g/75mL 10
DIETHYLAMINO
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUDAX111 100mg/1mL 100CUDAX123 200mg/3mL 50CUDAX153 500mg/3mL 50CUDAX156 500mg/6mL 50CUDAX12M15 2g/15mL 20CUDAX15M25 5g/25mL 20
% Organic Loading: 6.65
Exchange Capacity (meq/g): 0.310
Application:
Scavenger for acids, cycliccompounds, cholesterols, and otherlipid-type and compounds.
% Organic Loading: 9.70
Exchange Capacity (meq/g): 0.320
Application:
Scavenger for acids, cycliccompounds, cholesterols, and otherlipid-type and compounds.
% Organic Loading: 9.70
Exchange Capacity (meq/g): 0.320
Application:
Scavenger for acids, cycliccompounds, cholesterols, and otherlipid-type and compounds.
8/12/2019 Uct Tipos de Columna
36/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
36
www.unitedchem
.com
MOLECULAR
ANALYTE CHARGELOG P > 3 LOG P < 2
CN, NH2,PSA,
PHENYL,PI
GAS
SAMPLE
SOLIDLIQUID
LIPIDS, OIL ADDITIVES, CARBOHYDRATES,PHENOLS, OIL SOLUBLE VITAMINS,
PESTICIDES, HERBICIDES
DRUGS OF ABUSE, PHARMACEUTICALS,TDM, PESTICIDES
PARTITION COEFFICIENT
Po/w= (Coil /Cwater) EQUILIBRIUM. OIL IS USUALLY OCTANOL
LOG P SCALE
HYDROPHILIC (POLAR) < 1
MODERATELY POLAR 1-2
HYDROPHOBIC (NONPOLAR) > 2
TYPICAL APPLICATIONS:
+ -
CARBON SILICA GELFLUOROSIL
DIOL
LOG P < 1
NON POLAR, REVERSE PHASE EXTRACTION
AQUEOUS, BIOLOGICAL FLUIDS, BUFFERS
HYDROPHOBIC, LOG P > 2.5
INVOLVES VANDERWALLS/DISPERSION FORCES
INTERACTION BETWEEN SORBENT CH BONDS AND
ANALYTE CH BONDS
PROTONATED/NEUTRAL STATE,
AROMATICS, ALKYLCHAINS
POLAR OR NORMAL PHASE EXTRACTION
NON POLAR ORGANIC
HYDROPHILIC, LOG P < 2.5
INVOLVES HYDROGEN BONDING
AND DIPOLE/DIPOLE INTERACTIONS
AMINES, HYDROXYLS, CARBONYLS,
AROMATIC RINGS, HETERO ATOMS (O, S,
N, P)
NATUREOF SAMPLE
TYPES
OF FUNCTIONAL
GROUPS
BONDING
MECHANISM
NATURE
OF ANALYTE
MATRIX
TYPE
OF EXTRACTION
ANALYTE
CONFIGURATION
ANALYTE
POLARITY
SORBENT
TYPE
NON POLAR TO MODERATELY POLAR SOLVENTSWATER/ACETONITRILE/METHANOL MIXES
MEDIUM TO HIGH POLARITY SOLVENTSMETHANOL/ETHYL ACETATE/ACETONE/THF
MIXES
ELUTION SOLVENT:
A MAP TO OPTIMIZING SAMPLE CLEAN UP
C8- C30PS/DVD
C2- C7CYCLOHEXYL
ALUMINA
8/12/2019 Uct Tipos de Columna
37/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
ANION EXTRACTION COLUMNS
QUATERNARY AMINE WITH CHLORIDE COUNTER ION
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUQAX1L1 50mg/1mL 100CUQAX111 100mg/1mL 100CUQAX123 200mg/3mL 50CUQAX153 500mg/3mL 50CUQAX156 500mg/6mL 50CUQAX1M6 1g/6mL 30CUQAX12Z 200mg/10mL 50CUQAX15Z 500mg/10mL 50CUQAX12M15 2g/15mL 20
QUATERNARY AMINE WITH ACETATE COUNTER ION
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CAQAX111 100mg/1mL 100CAQAX123 200mg/3mL 50CAQAX1M6 1g/6mL 30CAQAX15M25 5g/25mL 20
QUATERNARY AMINE WITH HYDROXIDE COUNTER ION
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CHQAX1L1 50mg/1mL 100CHQAX111 100mg/1mL 100
CHQAX123 200mg/3mL 50CHQAX153 500mg/3mL 50CHQAX156 500mg/6mL 50CHQAX1M6 1g/6mL 30CHQAX12Z 200mg/10mL 50CHQAX12M15 2g/15mL 20CHQAX15M25 5g/25mL 20
POLYIMINE
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUPAX123 200mg/3mL 50CUPAX153 500mg/3mL 50CUPAX1M6 1g/6mL 30
% Organic Loading: 8.40
Exchange Capacity (meq/g): 0.250
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles.
% Organic Loading: 8.40
Exchange Capacity (meq/g): 0.250
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe acetate counter ion.
% Organic Loading: 8.40
Exchange Capacity (meq/g): 0.250
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe hydroxide counter ion.
% Organic Loading: 13.5
Exchange Capacity (meq/g): 0.88
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and otherelectrophiles.
ANY CHARGED COMPOUNDS
IONIC
2 BCX, PCX &COPOLYMERS
CATIONSANIONS
STRONG STRONG
QAX &COPOLYMERS
CCX, TAX, &COPOLYMERS
MUST BE ABLE TO BE CHARGED
AQUEOUS OR ORGANIC
ION EXCHANGE
INVOLVES IONIC EXCHANGE
ACIDS AND BASESANIONS: PHOSPHATES, CARBOXYLIC ACIDS,
SULFONIC ACIDSCATIONS: AMINES, PURINES, PYRIMIDINES
NATUREOF SAMPLE
TYPES
OF FUNCTIONAL
GROUPS
BONDING
MECHANISM
NATURE
OF ANALYTE
MATRIX
TYPE
OF EXTRACTION
ANALYTE
CONFIGURATION
ANALYTEPOLARITY
SORBENT
TYPE
BRISTOL, PA 19007
800-385-3153
www.unitedchem.com
2731 BARTRAM ROAD
UCT, Inc.
ACIDIC ELUTIONSOLVENT
BASIC ELUTIONSOLVENT
USING SOLID PHASE EXTRACTION
TYPICAL APPLICATIONS:
ELUTION SOLVENT:
NH , DEAE,PSA, PI, &
COPOLYMERS
WEAK WEAK
8/12/2019 Uct Tipos de Columna
38/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
38
www.unitedchem
.com
CLEAN-UP
ANION EXTRACTION COLUMNS
QUATERNARY AMINE WITH CHLORIDE COUNTER ION
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUQAX1L1 50mg/1mL 100CUQAX111 100mg/1mL 100CUQAX123 200mg/3mL 50CUQAX153 500mg/3mL 50CUQAX156 500mg/6mL 50CUQAX1M6 1g/6mL 30CUQAX12Z 200mg/10mL 50CUQAX15Z 500mg/10mL 50CUQAX12M15 2g/15mL 20
QUATERNARY AMINE WITH ACETATE COUNTER ION
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CAQAX111 100mg/1mL 100CAQAX123 200mg/3mL 50CAQAX1M6 1g/6mL 30CAQAX15M25 5g/25mL 20
QUATERNARY AMINE WITH HYDROXIDE COUNTER ION
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CHQAX1L1 50mg/1mL 100CHQAX111 100mg/1mL 100
CHQAX123 200mg/3mL 50CHQAX153 500mg/3mL 50CHQAX156 500mg/6mL 50CHQAX1M6 1g/6mL 30CHQAX12Z 200mg/10mL 50CHQAX12M15 2g/15mL 20CHQAX15M25 5g/25mL 20
POLYIMINE
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUPAX123 200mg/3mL 50CUPAX153 500mg/3mL 50CUPAX1M6 1g/6mL 30
% Organic Loading: 8.40
Exchange Capacity (meq/g): 0.250
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles.
% Organic Loading: 8.40
Exchange Capacity (meq/g): 0.250
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe acetate counter ion.
% Organic Loading: 8.40
Exchange Capacity (meq/g): 0.250
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and weakelectrophiles. Useful when chargeon ion being removed is weaker thanthe hydroxide counter ion.
% Organic Loading: 13.5
Exchange Capacity (meq/g): 0.88
Application:
Scavenger for acids and sulfonylchlorides, isocyanates and otherelectrophiles.
8/12/2019 Uct Tipos de Columna
39/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
CATION EXTRACTION COLUMNS
CARBOXYLIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUCCX1L1 50mg/1mL 100CUCCX111 100mg/1mL 100CUCCX123 200mg/3mL 50CUCCX153 500mg/3mL 50CUCCX156 500mg/6mL 50CUCCX1M6 1g/6mL 30CUCCX12Z 200mg/10mL 50CUCCX12M15 2g/15mL 20CUCCX15M25 5g/25mL 20
PROPYLSULFONIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUPCX111 100mg/1mL 100CUPCX123 200mg/3mL 50CUPCX153 500mg/3mL 50CUPCX156 500mg/6mL 50CUPCX12Z 200mg/10mL 50CUPCX15Z 500mg/10mL 50
TRIACETIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUTAX111 100mg/1mL 100CUTAX123 200mg/3mL 50CUTAX153 500mg/3mL 50CUTAX156 500mg/6mL 50CUTAX1M6 1g/6mL 30CUTAX110M75 10g/75mL 10
% Organic Loading: 9.10
Exchange Capacity (meq/g): 0.170
Application:
Scavenger for strong anions(Quaternary amines or metals)
% Organic Loading: 7.10
Exchange Capacity (meq/g): 0.180
Application:
Scavenger for amines, alcohols andother nucleophiles.
% Organic Loading: 7.61
Exchange Capacity: Anion: 0.17 Cation: 0.06Application:
Chelator for metal ions. i.e. tin palladium copper ruthinium chromium
nickel
8/12/2019 Uct Tipos de Columna
40/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
40
www.unitedchem
.com
CLEAN-UP
CATION EXTRACTION COLUMNS
BENZENESULFONIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUBCX1L1 50mg/1mL 100CUBCX111 100mg/1mL 100CUBCX123 200mg/3mL 50CUBCX153 500mg/3mL 50CUBCX156 500mg/6mL 50CUBCX1M6 1g/6mL 30CUBCX11Z 100mg/10mL 50CUBCX12Z 200mg/10mL 50CUBCX15Z 500mg/10mL 50CUBCX12M15 2g/15mL 20CUBCX110M75 10g/75mL 10
BENZENESULFONIC ACID - HIGH LOAD
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUBCX1HL1L1 50mg/1mL 100CUBCX1HL11 100mg/1mL 100CUBCX1HL23 200mg/3mL 50CUBCX1HL53 500mg/3mL 50CUBCX1HL56 500mg/6mL 50CUBCX1HLM6 1g/6mL 30CUBCX1HL1Z 100mg/10mL 50CUBCX1HL2Z 200mg/10mL 50CUBCX1HL5Z 500mg/10mL 50CUBCX1HL2M15 2g/15mL 20CUBCX1HL5M25 5g/25mL 20CUBCX1HL10M75 10g/75mL 10
% Organic Loading: 11.00
Exchange Capacity (meq/g): 0.320
Application:
Scavenger for amines, alcohols andother nucleophiles.
% Organic Loading: 11.00
Exchange Capacity (meq/g): 0.320
Application:
Scavenger for amines, alcohols andother nucleophiles.
8/12/2019 Uct Tipos de Columna
41/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
This sorbent is composed of a silica back-
bone with two types of functional chains
attached - an ion exchanger or polar chain
and a hydrophobic carbon chain. Our
copolymeric phases are produced in a way
to allow for equal parts of each functional
group to attach to the silica backbone. Thiscopolymerization technique yields reproducible
bonded phases and unique copolymeric
chemistries which allow the controlled use
of mixed mode separation mechanisms.This
type of dual chemistry is beneficial especially
when one is looking for both a neutral &
charged compound. This is common when a
neutral parent drug metabolizes & becomes
a charged compound.
Cations/Anions
Alkanes
Alkenes
Aromatics
1) Aqueous to disrupt
hydrophilic interactions.
2) Methanol to disrupt residualhydrophobic and hydrophilicinterferences.
1) Organic, possibly with some
aqueous to elute hydrophobicallybound analytes.
2) Aqueous buffer with a pH thatwould neutralize ionically boundanalytes or an aqueous with highionic strength or a solvent with acounter ion that would bindto sorbent.
CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS
Example of a Copolymeric Phase
Silica Backbone
Hydrophobic Chain
Ion Exchanger
O
Si
O
Si
O
Si
O
Si
O Si (CH2)3 NH+3
OH
OH
CnH2n+1Si
OH
OH
O
Analytes Washes Elutions
8/12/2019 Uct Tipos de Columna
42/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
42
www.unitedchem
.com
CLEAN-UP
COPOLYMERIC EXTRACTION COLUMNS
COPOLYMERIC SORBENTS & STRUCTURES
Sorbent Structure* pKa
Benzenesulfonic Acid (BCX2) -Si-(CH2)
2 SO
3H always charged
Strong Cation Exchange Column
Propylsulfonic Acid (PCX2) -Si-(CH2)
3SO
3H < 1
Strong Cation Exchange Column
Carboxylic Acid (CCX2) -Si-(CH2)
2COOH 4.8
Weak Cation Exchange Column
Quaternary amine (QAX2) -Si-(CH2)
3N+(CH
3)
3 always charged
Strong Anion Exchange Column
Aminopropyl (NAX2) -Si-(CH2)
3NH
3 + 9.8
Weak Anion Exchange Column
Cyanopropyl (CNP2) -Si-(CH2)3CNHydrophilic Exchange Column
Cyclohexyl (CYH2) -Si-(CH2)
Hydrophobic Exchange Column
*Each copolymeric sorbent also contains a carbon chain approximately equal to a C8 chain
MECHANISM OF COPOLYMERIC BONDING
Using a sample composed of a theoretical neutral parentdrug and its charged (acidic) metabolite, it is appliedat a pH of 6 (figure 1). At this pH, many amine groups are
positively charged. Since the column is also positivelycharged, compounds with this chemistry (cations)are repelled. Depending on the pKa of the metabolite,carboxylic acid groups may be negatively charged, allowingthe metabolite to bond to the positively charged sorbent.Since the column also possesses a hydrophobic chain,the neutral parent drug also bonds to the column.
Water or a weak aqueous buffer (pH6) washes awayhydrophilically bound interferences (figure 2). The columnis then dried, careful to free the column of any residualaqueous phase that would interfere with elution.
Example of Copolymeric Bonding
(CH2)n NH3+ CO
O
SorbentCompounds
8/12/2019 Uct Tipos de Columna
43/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP COPOLYMERIC EXTRACTION COLUMNS
Sample Applicationgure 1
Column Washgure 2
Elution 1gure 3
The hydrophobicallybound neutral parentdrug is eluted with asolvent of minimalpolarity, such ashexane/ethyl acetate -80:20.
Elution 2gure 4
The final elutionemploys an acid toneutralize the chargeof acidic analytes. Ionicinteraction is released,and analytes are elutedin an appropriate solventmixture.
NH3+
CO
O
O
NH3+
NH+
NH+
O
-
Sorbent
Sorbent
Sorbent
Metabolite
Aqueous Buffer
Parent Drug
NH3+
CO
O
O
NH3
SH
HO
OH
+
-Sorbent
Sorbent
Sorbent
Metabolite
Aqueous Buffer
Hydrophilically
Bound
CompoundsParent Drug
NH3+
CO
O
NH3+
O
-
Sorbent
Sorbent
Sorbent
Parent Drug
Hexane / Ethyl
Acetate
80:20
NH3+
CHO
O
NH3+
Sorbent
Sorbent
Sorbent
Metabolite
Strong Acidic
Solution
pH 2-3
Anion Exchange Sorbent Cation Exchange Sorbent
Goal pH Goal pH
WASH
Elution
Percent of Compound in Ionic State
Functionality Ionization
To promote
bonding between
sorbent and analyte
pH units away from pKa
2 < pKa 1 < pKa at pKa 1 > pKa 2 > pKa
Acid Anionic (-) 1 9 50 91 99
Base Cationic (+) 99 91 50 9 1
To disrupt
bonding between
sorbent and analyte
To promote
bonding between
sorbent and analyte
To promote
bonding between
sorbent and analyte
> analyte pKa
or
< sorbent pKa
> analyte pKa
or
< sorbent pKa
> analyte pKa
or
< sorbent pKa
> analyte pKa
or
< sorbent pKa
8/12/2019 Uct Tipos de Columna
44/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
44
www.unitedchem
.com
CLEAN-UP
COPOLYMERIC EXTRACTION COLUMNS
HYDROPHOBIC PLUS CYCLOHEXYL
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUCYH256 500mg/6mL 50CUCYH2M6 1g/6mL 30
HYDROPHOBIC PLUS PROPYLSULFONIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUPCX2L1 50mg/1mL 100CUPCX211 100mg/1mL 100CUPCX223 200mg/3mL 50CUPCX256 500mg/6mL 50CUPCX22Z 200mg/10mL 50
HYDROPHOBIC PLUS CARBOXYLIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUCCX2L1 50mg/1mL 100CUCCX211 100mg/1mL 100CUCCX223 200mg/3mL 50CUCCX256 500mg/6mL 50
HYDROPHOBIC PLUS BENZENESULFONIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUBCX2L1 50mg/1mL 100CUBCX211 100mg/1mL 100CUBCX223 200mg/3mL 50CUBCX253 500mg/3mL 50CUBCX256 500mg/6mL 50CUBCX2M6 1g/6mL 30CUBCX21Z 100mg/10mL 50CUBCX22Z 200mg/10mL 50CUBCX25Z 500mg/10mL 50
OCTADECYL PLUS BENZENESULFONIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUBCX35Z 500mg/10mL 50CUBCX32M15 2g/15mL 20
% Organic Loading: N/A
Exchange Capacity (meq/g): N/A
Application:
Dual functionality for phenols andhydrophobic compounds.
% Organic Loading: 14.62
Exchange Capacity (meq/g): 0.114
Application:
Dual functionality for weak basesand hydrophobic compounds.
% Organic Loading: 12.50
Exchange Capacity (meq/g): 0.105
Application:
Dual functionality for strong base andhydrophobic compounds.
% Organic Loading: 12.30
Exchange Capacity (meq/g): 0.072
Application:
Dual functionality for strong base andhydrophobic compounds.
% Organic Loading: 12.30
Exchange Capacity (meq/g): 0.072
Application:
Dual functionality for strong base andhydrophobic compounds.
8/12/2019 Uct Tipos de Columna
45/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
CLEAN-UP
COPOLYMERIC EXTRACTION COLUMNS
HYDROPHOBIC PLUS QUATERNARY AMINE
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUQAX2L1 50mg/1mL 100CUQAX211 100mg/1mL 100CUQAX223 200mg/3mL 50CUQAX253 500mg/3mL 50CUQAX256 500mg/6mL 50CUQAX2M6 1g/6mL 30CUQAX21Z 100mg/10mL 50CUQAX22Z 200mg/10mL 50CUQAX25Z 500mg/10mL 50
% Organic Loading: 13.60
Exchange Capacity (meq/g): 0.160
Application:
Dual functionality for weak acids andhydrophobic compounds.
HYDROPHOBIC PLUS AMINOPROPYL
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUNAX2L1 50mg/1mL 100CUNAX211 100mg/1mL 100CUNAX223 200mg/3mL 50CUNAX253 500mg/3mL 50CUNAX256 500mg/6mL 50CUNAX2M6 1g/6mL 30CUNAX21Z 100mg/10mL 50CUNAX22Z 200mg/10mL 50CUNAX25Z 500mg/10mL 50CUNAX22M15 2g/15mL 20CUNAX25M25 5g/25mL 20CUNAX210M75 10g/75mL 10
% Organic Loading: 12.3
Exchange Capacity (meq/g): 0.163
Application:
Dual functionality for strong acidsand hydrophobic compounds.
8/12/2019 Uct Tipos de Columna
46/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
46
www.unitedchem
.com
CLEAN-UP
COVALENT EXTRACTION COLUMNS
COVALENT
Covalent sorbent has aldehyde functional groups that are bound to the silicabackbone by a hydrocarbon chain. The aldehyde group will react selectively
with compounds containing a primary amine. A formal bond is createdbetween the stationary support and the primary amine containing material.
MECHANISM OF COVALENT BONDING
The primary amine in the sample performs a nucleophilic attack on the aldehydefunctional group attached to the support. This results in a Schiff base, with theamine immobilized on the stationary support. This chemistry can be utilized tobind proteins, such as antibodies, to the support, allowing highly specific extractions.
THIOPROPYL
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
CUTHX1L1 50mg/1mL 100CUTHX111 100mg/1mL 100CUTHX123 200mg/3mL 50CUTHX153 500mg/3mL 50CUTHX156 500mg/6mL 50CUTHX1M6 1g/6mL 30CUTHX11Z 100mg/10mL 50CUTHX12Z 200mg/10mL 50CUTHX15Z 500mg/10mL 50CUTHX12M15 2g/15mL 20CUTHX15M25 5g/25mL 20CUTHX110M75 10g/75mL 10
% Organic Loading: 6.50
Application:
Scavenger for alkylating agents,alcohols and amines.
8/12/2019 Uct Tipos de Columna
47/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
STYRE SCREEN POLYMERIC RESIN EXTRACTION COLUMNS
8/12/2019 Uct Tipos de Columna
48/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
48
www.unitedchem
.com
STYRE SCREEN
POLYMERIC RESIN EXTRACTION COLUMNS
Styre Screenextraction columns contain an ultra clean, highly cross-linked styrene and divinylbenzene copolymersorbent that is functionalized with both a reverse phase, hydrophobic component and a strong cation exchanger.High and reproducible recoveries for acidic, neutral and basic compounds are achievable with a single column. TheStyre Screenparticles have an average particle size of 30 microns and a very high analyte capacity making them idealfor standard solid phase extraction applications. The increased analyte capacity means that less sorbent bed mass isneeded which results in faster flow rates and less solvent use. Higher throughput and less solvent waste disposal
translate into significant savings in both time and money. In addition, no conditioning steps are required for mostdrugs of abuse applications.
DBX - BENZENESULFONIC ACID + C18
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
SSDBX031 30mg/1mL 100SSDBX033 30mg/3mL 50SSDBX056 50mg/6mL 50
DVB - POLYSTYRENE DIVINYLBENZENE
Part Number Sorbent Amount/ Unit per Tube Volume Pack
SSDVB031 30mg/1mL 100SSDVB033 30mg/3mL 50SSDVB056 50mg/6mL 50
BCX - ION EXCHANGE
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
SSBCX031 30mg/1mL 100SSBCX033 30mg/3mL 50SSBCX056 50mg/6mL 50
C18 - REVERSE PHASE
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
SSC18031 30mg/1mL 100SSC18033 30mg/3mL 50SSC18056 50mg/6mL 50
CCX - CARBOXYLIC ACID
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
SSCCX031 30mg/1mL 100SSCCX033 30mg/3mL 50SSCCX056 50mg/6mL 50
QAX - QUATERNARY AMINE
Part Number Sorbent Amount/ Unit per
Tube Volume Pack
SSQAX031 30mg/1mL 100SSQAX033 30mg/3mL 50SSQAX056 50mg/6mL 50
Application:
Dual functionality for weak acidsand hydrophobic compounds.
Application:N/A
Application:
Scavenger for amines, alcoholsand other nucleophiles.
Application:
Removes hydrophobic impurities,de-salting and purification ofhydrophobic compounds.
Application:
Scavenger for strong anions.(Quaternary amines or metals)
Application:
Removes large or morehydrophobic compounds.
8/12/2019 Uct Tipos de Columna
49/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
SOLID PHASE EXTRACTION COLUMN METHOD DEVELOPMENT KITS
UCT understands that the optimum
sorbent for any given separation
cannot always be chosen empirically
and that the cost of purchasing
individual sorbents for screening
purposes can be prohibitive. We offer
the following kits at prices designed
to reduce the cost of assay development:
Non-Polar Phases
Polar Phases
Ion Exchange Phases
Copolymeric Phases Pharmaceutical Phases
Toxicology Phases
8/12/2019 Uct Tipos de Columna
50/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
50
www.unitedchem
.com
SOLID PHASE EXTRACTION COLUMN
METHOD DEVELOPMENT KITS
NON-POLAR PHASES, ENDCAPPED
Part Number: MDK-NPE-I Total Number of Tubes: 140 7 packages / 10 each
CEC18111 CECn4111 CEC08111CECYH111 CEPHY111CEC02111 CEC30111
POLAR PHASES
Part Number: MDK-PU-I Total Number of Tubes: 80 8 packages / 10 each
CEC02111 CUDOL111 CUPSA111CUCNP111 CUSIL111 CUDAX111CECNP111 CUNAX111
COPOLYMERICPart Number: MDK-PU/1EX-11 Total Number of Tubes: 60 6 packages / 10 each
CUCNP211 CUCCX211CUNAX211 CUPCX211CUQAX211 CUBCX211
ION EXCHANGE PHASES
Part Number: MDK-IEX-I Total Number of Tubes: 70 7 packages / 10 each
CUNAX111 CUCCX111
CUPSA111 CUPCX111CUDAX111 CUBCX111CUQAX111
TOXICOLOGY PHASES
Part Number: MDK-TOX-111 Total Number of Tubes: 40 8 packages / 5 each
CSDAU203 CEC02123CSTHC203 CEC08123CUSIL123 CEC18123CECNP123 CUC18123
PHARMACEUTICAL PHASES
Part Number: MDK-PHM-111 Total Number of Tubes: 40 8 packages / 5 each
CEC08123 CEC18123CUBCX223 CUCCX123CUNAX223 CUQAX123CEC02123 CUCNP123
This kit contains ten 100 mg/1 ml tubes of each of seven non-polar phaseswhich include the endcapped hydrophobic phases, for C2, C4, C8, C18 and C30along with cyclohexyl (CYH), and phenyl (PHY) phases.
This kit contains ten 100 mg/1 ml tubes of each of eight phases with polarcharacteristics, including C2, cyanopropyl (CNP), diol (DOL), silica (SIL),primary amine (aminopropyl; NAX), secondary amine (aminoethyl; PSA),tertiary amine (diethylamino; DAX).
This kit contains ten 100 mg/1 ml tubes of each of six mixed mode phases containing anon-polar and polar or ion exchange functionality. The non-polar functionality in each casehas an effective chain length of a C8. The polar or ion exchange functionality of each phaseconsists of one of the following: cyanopropyl (CNP2), primary amine (aminopropyl; NAX2),quaternary amine (QAX2), carboxylic acid (CCX2), propylsulfonic acid (PCX2), andbenzenesulfonic acid (BCX2).
This kit contains ten 100 mg/1 mL tubes of each of seven ion exchange phases including
primary amine (aminopropyl; NAX), secondary amine (aminoethyl; PSA), tertiary amine(diethylamino; DAX), quaternary amine (QAX), carboxylic acid (CCX), propylsulfonic acid(PCX), and benzenesulfonic acid (BCX) phases.
This kit contains five 200 mg/3 ml tubes of each of eight phases. The two standard phasesused for drugs of abuse testing are CSDAU and CSTHC. Other phases commonly used intoxicology are the polar and non-polar phases. The polar phases are unbonded silica andcyanopropyl (CNP); the non-polar phases are endcapped, C2, C8, and both endcapped andunendcapped C18.
This kit contains five 200 mg/3 ml tubes of each of eight phases that are most often selectedfor pharmaceutical applications. Two copolymeric phases having the dual functionalities ofnon-polar C8 and either benzenesulfonic acid (BCX) or aminopropyl (NAX). The remainingcolumns are phases which include three endcapped, hydrophobic phases (C2, C8, C18), twoion exchange phases (CCX, QAX), and one polar phase (CNP).
8/12/2019 Uct Tipos de Columna
51/80U C
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
SELECTRASORB
BULK SORBENTS PACKING MATERIAL
COPOLYMERIC BONDED PHASESFOR DRUG ABUSE TESTING
Description Part Number Sizes
CSDAU
CSDAU00X 10g CSDAU00C 100g CSDAU00K 1kg
CSTHC
CSTHC00X 10g CSTHC00C 100g CSTHC00K 1kg
8/12/2019 Uct Tipos de Columna
52/80U C T
UCT | 2731 Bartram Rd | Bristol, PA 19007 | USAP.800.385.3153 | 215.781.9255 | F.215.785.1226
www.unitedchem.com
52
www.unitedchem
.com
SELECTRASORB
BULK SORBENTS
HYDROPHOBIC
Description Part Number Part Number Sizes
Endcapped Unendcapped
C8, OCTYL
CEC0800X CUC0800X 10g CEC0800C CUC0800C 100g CEC0800K CUC0800K 1kg
C18, OCTADECYL
Tri-Functional
CEC1800X CUC1800X 10g CEC1800C CUC1800C 100g CEC1800K CUC1800K 1kg
HYDROPHILIC
Description Part Number Part Number Sizes
Endcapped Unendcapped
CN,CYANOPROPYL
CECNP00X CUCNP00X 10g CECNP00C CUCNP00C 100g CECNP00K CUCNP00K 1kg
UNBONDED SILICA
40-63 m CUSIL00X 10g CUSIL00C 100g CUSIL00K 1kg
PHARMA-SIL
40-63 m PHSIL00X 10g PHSIL00C 100g PHSIL00K 1kgHigh Surface Activity Silica
40-63 m HSSIL00X 10g HSSIL00C 100g HSSIL00K 1kg
DIOL
CUDOL00X 10g CUDOL00C 100g CUDOL00K 1kg
FLORISIL
CUFLS00X 10g CUFLS00C 100g CUFLS00K 1kg
ALUMINA, ACIDIC
CUALA00X 10g CUALA00C 100g CUALA00K 1kg
ALUMINA, BASIC
CUALB00X 10g CUALB00C 100g CUALB00K 1kg
ALUMINA, NEUTRAL
CUALN00X 10g CUALN00C 100g CUALN00K 1kg
COPOLYMERIC
Description Part Number Part Number Sizes
Endcapped Unendcapped
BENZENESULFONIC
ACID + C8
CUBCX20X 10g CUBCX20C 100g CUBCX20K 1kg
CARBOXYLIC
ACID + C8
CUCCX20X 10g CUCCX20C 100g CUCCX20K 1kg
QUATERNARY
ACID +