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Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1 , Axel Tiessen 2 , Felipe Ascencio 1 , Carlos Angulo 1 , and Gracia Gómez-Anduro 1* 1 Centro de Investigaciones Biológicas del Noroeste (CIBNOR) Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23096, México Email: [email protected] ; [email protected] ; [email protected] 2 Departamento de Ingeniería Genética, CINVESTAV, Unidad Irapuato, Km. 9.6 Libramiento Norte, 36821 Irapuato, México Email: [email protected] * Corresponding author: Gracia Gómez-Anduro Email address: [email protected] Supplementary Material 2 Bioinformatics analysis of β-Glucosidases promoters. The sequences of the putative promoter region (2000 pb upstream start codon) of pZmBGlu2, 5, 7, 12. Showing the transcription factors binding sites. Where one is the most distant position and 2000 is the nearest to the start codon. Each analysis was performed using
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Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

Dec 16, 2015

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Page 1: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas1, Axel Tiessen2, Felipe Ascencio1, Carlos Angulo1, and Gracia Gómez-Anduro1*

1 Centro de Investigaciones Biológicas del Noroeste (CIBNOR) Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23096, México Email: [email protected]; [email protected]; [email protected] Departamento de Ingeniería Genética, CINVESTAV, Unidad Irapuato, Km. 9.6 Libramiento Norte, 36821 Irapuato, México Email: [email protected]*Corresponding author: Gracia Gómez-Anduro Email address: [email protected]

Supplementary Material 2Bioinformatics analysis of β-Glucosidases promoters. The sequences of the putative promoter region (2000 pb upstream start codon) of pZmBGlu2, 5, 7, 12. Showing the transcription factors binding sites. Where one is the most distant position and 2000 is the nearest to the start codon. Each analysis was performed using databases for maize, in a database reported by (Chang et al. 2008).

Page 2: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

All cis Elements

Page 3: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

-300 CORE (PLACE)

pZmBGlu2

pZmBGlu12

Page 4: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

ABI4 (PLACE)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 5: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

ANAERO1CONSENSUS (PLACE)

pZmBGlu2

pZmBGlu5

pZmBGlu7

Page 6: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

ANAERO2CONSENSUS (PLACE)

pZmBGlu2

Page 7: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

pZmBGlu2

ANAERO3CONSENSUS (PLACE)

Page 8: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

C1MOTIFZMBZ2 (PLACE)

Page 9: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

DOFCOREZM (PLACE)

Page 10: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

DRE1COREZMRAB17 (PLACE)

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 11: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

DRE2COREZMRAB17 (PLACE)

pZmBGlu5

Page 12: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

DRECRTCOREAT (PLACE)

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 13: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

INTERLOWER (PLACE)

pZmBGlu5

pZmBGlu7

pZmBGlu12

pZmBGlu2

Page 14: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

MYBPZM (PLACE)

pZmBGlu2

pZmBGlu24

pZmBGlu7

Page 15: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

POLASIG3 (PLACE)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 16: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

QELEMENTZM13 (PLACE)

pZmBGlu2

pZmBGlu5

pZmBGlu12

Page 17: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

pZmBGlu7

TATAPVRNALEU (PLACE)

Page 18: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

Dof1/MNB1A (JASPER)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Dof1/MNB1A (TRANSFAC)

Page 19: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Dof2 (JASPER)

Dof2 (TRANSFAC)

Page 20: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

Dof3 (JASPER)

Dof3 (TRANSFAC)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 21: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

PBF (JASPER)

PBF (TRANSFAC)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 22: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

O2 (TRANSFAC)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12

Page 23: Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen.

P (TRANSFAC)

pZmBGlu2

pZmBGlu5

pZmBGlu7

pZmBGlu12