Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populations as inferred from complete mtDNA genome sequences Steven M Carr Terra Nova Genomics, Inc. & Dept of Biology, Memorial University of Newfoundland St John's NL, Canada 2 nd International Conference on Oceanography Las Vegas NV, 21 July 2014
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Genomic Biotech for Environmental Monitoring: Phylogeography & connectivity among marine populations
as inferred from complete mtDNA genome sequences
Steven M CarrTerra Nova Genomics, Inc. &
Dept of Biology, Memorial University of NewfoundlandSt John's NL, Canada
2nd International Conference on OceanographyLas Vegas NV, 21 July 2014
Two Genomes
nucDNA genome2,850,000,000 bp
20,500 genes
mtDNA genome~17,000 bp38 genes
DNA Sequencing on a MicroarrayComplete mtDNA genome in one experiment
[ SM Carr et al. 2008. Comp Biochem Physiol D 3:1-11 ]
Molecular Velcro: Multi-species ArkChip(Three fish, three mammals, one bird: 115,984 bp)
Information content of DNA microarrays:7 species X 80 individuals / spp X 16.5 Kbp @ = 9.2 Mbp
Atlantic Cod(Gadus morhua)
Newfoundland Caribou(Rangifer tarandus)
Harp Seals(Pagophilus groenlandicus)
Atlantic Wolffish(Anarhicas lupus)
Harp Seal pupping & breeding grounds
Gulf
Front
GreenlandSea
WhiteSea
Genomic Phylogeography of Pagophilus16.5 Kbp @ from 83 Harp seals