TU/e eindhoven university of technology /department of chemical engineering and chemistry oftware and Internet Resourc for Capillary Electrophoresi Jetse Reijenga 1 * and Hian Kee Lee 2 indhoven University of Technology, Netherlan 2 National University of Singapore title titel
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TU/e eindhoven university of technology / department of chemical engineering and chemistry Software and Internet Resources for Capillary Electrophoresis.
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TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
Software and Internet Resources for Capillary Electrophoresis
Jetse Reijenga1* and Hian Kee Lee2
1 Eindhoven University of Technology, Netherlands2 National University of Singapore
titletitel
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
• Communication• CE literature search• Software for parameter estimation• Software for simulation• Data analysis software• Internet information resources
content
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
E-mail discussion list: [email protected] 480 CE users, 1-25 e-mails/week
Subscribe by sending to [email protected] the message:SUBSCRIBE CZE-ITP firstname lastname
• joint 20 MB web for exp results (with e.g. F2S.COM)• virtual 50 MB drive on internet (e.g. FreeDrive)• e-mail• Chat (e.g. ICQ)• Internet telephony (e.g. DialPad)• Webcam (e.g. Netmeeting)
• http:// and @ broaden our horizon for cooperation• An estimated value is better than no value• Visualization as 1st step in method development• The library is a virtual one• There are no borders for information exchange
Conclusions
Conclusions
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
The Use of Fluorosurfactant FC-129 in Micellar Electrokinetic Capillary
ChromatographyRonny de Ridder1,2, Francesco Damin1, Jetse Reijenga2 and Marcella Chiari1
1. Institute of Biocatalysis and Molecular Recognition, C.N.R., Milano, Italy
2. Laboratory of Instrumental Analysis, Eindhoven University of Technology,The Netherlands
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
FC-129
N-ethyl-N-[(heptadecafluorooctyl)sulfonyl]glycine, K salt
[CAS 2991-51-7], Aldrich (Steinheim, Germany)
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
CMCFC-129 = 0.5 mM
CMCSDS = 8 mM
CMC determination
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
Experimental
Spectra-Phoresis 1000TM
(Thermo Separation Products, Freemont, CA, USA)
Fused silica, 50m ID, 375 m OD Lt=400 mm, Ld=320 mm, V=20 kVUV detection @ 200 nmInjection 5.17.103 N/m2 pressure, 4 s BGE: 12.5 mM Sodium tetraborate pH 9.9
TU/eeindhoven university of
technology
/department of chemical engineering and chemistry
Results EOF and Micelle Mobility
EOFmobility
MICELLEmobility
Migrationwindow
Mean sd Mean sd15 mM SDS 63.1 0.6 -39.9 0.8 2.720 mM SDS 65.8 -40.4 2.615 mM FC-129 60.3 0.0 -38.6 0.8 2.820 mM FC-129 59.1 0.7 -39.4 0.6 3.0