Page 2
Tracheal Development
DT
LT
DB
Page 3
Dysfusion (Dys) is a bHLH-PAS Protein
bHLH PAS-1 PAS-2
Page 4
Dysfusion Is Expressed In Fusion Cells
DT
DB
LT
Btl-GAL4, UAS-lacZ Dys
Page 5
dys Mutants
dys
3
dys
2
bHLH PAS-1 PAS-2
Mab 2A12
WT dys
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How dys expression is regulated?
How Dys:Tgo regulates down-stream target genes?
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Dysfusion is Expressed in Mutiple Cell Types
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Identify dys Enhancer
Page 9
Identify dys Fusion Cell Enhancer
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How dys expression is regulated?
•Is dys auto-regulated by Dys:Tgo?
N283-GFP,dys/Tm3, twi-gal4, UAS-GFPC1.7-GFP, dys/TM3, twi-gal4, UAS-GFP
•Is dys regulated by esg?esg/cyo, twi-gal4, UAS-GFP; N283esg/cyo, twi-gal4, UAS-GFP; C1.7
•Identify other transcription factors binding sites in dys enhancer.
Identify dys fusion cells enhancer in C1.7 and G1.7
Ongoing……
Page 11
Summary of dys Function
N
TC GTG
members only (Protein trafficking)
Trachealess (Migration)
CG13196, E-cadherin (cell adhesion)
CG15252 (unknown function)
TgoDys
TgoDys
TgoDys
Identified dys Targets
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Two TCGTG Sites are Required to Activates CG13196
Page 13
Two TCGTG Sites of CG13196 are Conserved
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Two TCGTG Sites are Required to Activates CG15252
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Two TCGTG Sites of CG15252 are Conserved
Page 16
Potential Co-activator of Dys:Tgo is Required to Turn on Gene Expression in Additional Fusion Cells