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Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE) *Upstream Promoter Element
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Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Dec 19, 2015

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Page 1: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Transcription in EukaryotesClass II promoters:

*Core Promoter

TATA box

TFIIB Recognition Element (BRE)

Initiator (Inr)

Downstream Promoter Element (DPE)

*Upstream Promoter Element

Page 2: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Transcription Initiation in Eukaryotes

General Transcription Factors and the Pre-initiation complex:

Page 3: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Transcription Initiation in EukaryotesTBP binding:

Page 4: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Transcription Initiation in Eukaryotes

Page 5: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Transcription Initiation in Eukaryotes

Page 6: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Schematic ModelElongation of Transcription in Eukaryotes

Page 7: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Polyadenylation

*CstF

*CPSF

*PAP

*PABP

RNA processing in eukaryotesAddition of poly A tail

Addition of 5’cap

Splicing

Page 8: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Torpedo model for transcription termination

Page 9: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Formation of the 5’ cap

*RNA triphosphatase

*guanylyl transferase

*methyl transferase

Transcript Processing

Page 10: Transcription in Eukaryotes Class II promoters: *Core Promoter TATA box TFIIB Recognition Element (BRE) Initiator (Inr) Downstream Promoter Element (DPE)

Functions of the Cap and Tail

ProtectionTranslatability TransportSplicing