Towards A Field Guide to the Microbes Jonathan A. Eisen University of California, Davis Twitter: @phylogenomics AAAS Annual Meeting 2012 February 17, 2012 Saturday, February 18, 12
May 19, 2015
Towards A Field Guide to the Microbes
Jonathan A. EisenUniversity of California, Davis
Twitter: @phylogenomics
AAAS Annual Meeting 2012February 17, 2012
Saturday, February 18, 12
Embargoes
• Note - slides will be posted to slideshare• Post anything about this you want• Discuss, blog, tweet, critique, mock,
whatever• Just don’t keep it secret
Saturday, February 18, 12
I. The Story of a Bird
Saturday, February 18, 12
4
Saturday, February 18, 12
4
Saturday, February 18, 12
Saturday, February 18, 12
Saturday, February 18, 12
Saturday, February 18, 12
8
Saturday, February 18, 12
9
Saturday, February 18, 12
Robin in London Examples
Saturday, February 18, 12
Saturday, February 18, 12
MICROBES
Saturday, February 18, 12
MICROBES
Saturday, February 18, 12
II. More on Field Guides
Saturday, February 18, 12
What is a Field Guide?
• Takes knowledge about taxonomy & diversity of organisms (or other entities) and converts it into a simple, portable, representation.
• Covers ranges, niches, phenotypes, genetics, means of identification, etc.
• Can include a checklist.• Used by amateurs & professionals.
Saturday, February 18, 12
Diversity of Guild Guides
from Diane Schmidt at http://www.library.illinois.edu/bix/fieldguides/index.html
Saturday, February 18, 12
Saturday, February 18, 12
Diversity of Guild Guides
from Diane Schmidt at http://www.library.illinois.edu/bix/fieldguides/index.html
Saturday, February 18, 12
Diversity of Guild Guides
from Diane Schmidt at http://www.library.illinois.edu/bix/fieldguides/index.html
Saturday, February 18, 12
A Field Guide to Bacteria
http://www.amazon.com/Field-Guide-Bacteria-Comstock-Book/dp/0801488540
Saturday, February 18, 12
Other Microbial Field Guides
Saturday, February 18, 12
What is a Field Guide?
• Takes knowledge about taxonomy & diversity of organisms (or other entities) and converts it into a simple, portable, representation.
• Covers ranges, niches, phenotypes, genetics, means of identification, etc.
• Can include a checklist.• Used by amateurs & professionals.
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
How many species?
Saturday, February 18, 12
Culturing Microbes
Saturday, February 18, 12
Culturing Microbes
Saturday, February 18, 12
Culturing Microbes
Saturday, February 18, 12
Mendeley Group
http://www.mendeley.com/groups/1359493/number-of-species-of-bacteria-and-archaea/
Saturday, February 18, 12
Cataloguing Types w/ DNA
• rRNA surveys• metagenomics • other metaomics
• Key issues• Phylogenetic classification• Reference data limited outside of rRNA• Phylogenetic diversity vs. OTUs• What is a species/OTU?
Saturday, February 18, 12
Metagenomic Phylotpying
rRNA Phylotyping in Sargasso Sea Metagenomic
Metagenomic Data
Venter et al., Science 304: 66. 2004
Saturday, February 18, 12
Metagenomic Phylotyping
0
0.1250
0.2500
0.3750
0.5000
Alphapro
teobacteria
Betap
roteobacteria
Gamm
aproteobacteria
Epsilo
nproteobacteria
Deltapro
teobacteria
Cyanobacteria
Firmicutes
Actinobacteria
ChlorobiCFB
Chloroflexi
Spirochaetes
Fusobacteria
Deinococcus-Th
ermus
Euryarchaeota
Crenarchaeota
Sargasso Phylotypes
Wei
ghte
d %
of C
lone
s
Major Phylogenetic Group
EFGEFTuHSP70RecARpoBrRNA
Venter et al., Science 304: 66. 2004
Saturday, February 18, 12
AMPHORA
Wu and Eisen Genome Biology 2008 9:R151 doi:10.1186/gb-2008-9-10-r151
Saturday, February 18, 12
Wu and Eisen Genome Biology 2008 9:R151 doi:10.1186/gb-2008-9-10-r151
Saturday, February 18, 12
RecAGOS 1
GOS 2
GOS 3
GOS 4
GOS 5
RecA
Wu D, Wu M, Halpern A, Rusch DB, Yooseph S, et al. (2011) Stalking the Fourth Domain in Metagenomic Data: Searching for, Discovering, and Interpreting Novel, Deep Branches in Marker Gene Phylogenetic Trees. PLoS ONE 6(3): e18011. doi:10.1371/journal.pone.0018011
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018011Saturday, February 18, 12
RpoB Too
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• EMP• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• METADATA• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
Phylogenetic Diversity
• Phylogenetic diversity poorly sampled
• GEBA project at DOE-JGI correcting this
Saturday, February 18, 12
AMPHORA ALL
Kembel et al. PLoS One 2011
Saturday, February 18, 12
Saturday, February 18, 12
Wu and Eisen Genome Biology 2008 9:R151 doi:10.1186/gb-2008-9-10-r151
Saturday, February 18, 12
New Markers
Dongying Wu et al. in prep
Phylogenetic group Genome Number Gene Number Maker Candidates
Archaea 62 145415 106
Actinobacteria 63 267783 136
Alphaproteobacteria 94 347287 121
Betaproteobacteria 56 266362 311
Gammaproteobacteria 126 483632 118
Deltaproteobacteria 25 102115 206
Epislonproteobacteria 18 33416 455
Bacteriodes 25 71531 286
Chlamydae 13 13823 560
Chloroflexi 10 33577 323
Cyanobacteria 36 124080 590
Firmicutes 106 312309 87
Spirochaetes 18 38832 176
Thermi 5 14160 974
Thermotogae 9 17037 684
Saturday, February 18, 12
Phylosift
• New version of Amphora• Developed by Aaron Darling, Holly Bik,
Erick Matsen, Guillaume Jospin, Dongying Wu, et al.
• http://phylosift.wordpress.com/tag/github/• Supported by DHS, etc.• Will work for more markers, viruses,
eukaryotes ...
Saturday, February 18, 12
Major phyla of bacteria & archaea
No cultures
Some cultures
Saturday, February 18, 12
GEBA Uncultured
46
Number of SAGs from Candidate Phyla
OD
1
OP
11
OP
3
SA
R4
06
Site A: Hydrothermal vent 4 1 - -Site B: Gold Mine 6 13 2 -Site C: Tropical gyres (Mesopelagic) - - - 2Site D: Tropical gyres (Photic zone) 1 - - -
Sample collections at 4 additional sites are underway.
Phil Hugenholtz
Saturday, February 18, 12
No cultures
Some cultures
Functional Diversity?
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation
Saturday, February 18, 12
Citizen Microbiology
http://www.microbe.net/citizen-science-projects/CITIZENMICROBIOLOGY.ORG
Saturday, February 18, 12
What is needed?
• Catalog of types of organisms• Biogeography (space & time); ranges• Niche information• Phenotypes and functions• Genetic diversity• Means for identification• Amateur participation• BONUS: Openness
Saturday, February 18, 12
What is needed?
• Open publications• Open data• Open tools• Open discussion
http://www.openmicrobiome.org/
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Plant/Animal Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Microbial Field Studies
Saturday, February 18, 12
Saturday, February 18, 12
MICROBES
Saturday, February 18, 12
Acknowledgements
• $$$• DOE• NSF• GBMF• Sloan• DARPA
• People, places• DOE JGI: Eddy Rubin, Phil Hugenholtz et al.• UC Davis: Aaron Darling, Dongying Wu• Other: Jessica Green, Katie Pollard, Martin Wu,
Tom Slezak, Jack Gilbert
Saturday, February 18, 12