Tosoh Bioscience llc - Phenomenex · 2008. 10. 16. · CH0-7951 07642 ODS-120T/A Guard column 21.5 7.5 10 CH0-8123* 19308 Guard cartridge holder 2.0 1.0 — oRdeRing infoRmation *

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TSKgel Bonded Functional End Particle Carbon Pore Exclusion Application/FeaturesColumn Phase Group Capping Size(µm) Load% Size(Å) Limit(Da)*ODS-80TM Monomeric C18 High 5,10,20 15 80 6,000 LowMWpeptides,peptidemapping,pharmaceuticals,nucleicacid

fragmentsODS-80TS Monomeric C18 Complete 5,10,20 15 80 6,000 AsODS-80TM,butmorestableathigherpHOctyl-80TS Monomeric C8 Complete 5 11 80 6,000 AsODS-80TM,butfasterkineticsthanODS.Lowerhydrophobic

selectivity.CN-80TS Monomeric Cyano Complete 5 8 80 6,000 AlternativetoODSandOctylcolumnsforanalysisofpolarcompoundsODS-120T Polymeric C18 High 5,10,20 22 120 10,000 ExpandsODS-80TMapplicationsforpeptidesandsmallproteinsODS-120A Polymeric C18 None 5,10 20 120 10,000 PolyaromatichydrocarbonsSuper-ODS Polymeric C18 Complete 2 8 110 20,000 High-throughputanalysesofpeptides,trypticdigestsandlowMWSuper-Octyl Polymeric C8 Complete 2 5 110 20,000 pharmaceuticals,purinesandpyrimidines,nucleosides,nucleotidesSuper-Phenyl Polymeric C6H5 Complete 2 3 110 20,000OligoDNARP Monomeric C18 None 5 10 250 165,000 Specialtycolumnforanalysisandpreparativepurificationof

oligonucleotides,RNAandDNA-fragmentsTMS-250 Monomeric C1 Complete 10 5 250 200,000 SpecialtycolumnsforproteinseparationsODS-100V Monolayer C18 Complete 5 15 100 — StrongerretentionforpolarcompoundsODS-100Z Monolayer C18 Complete 5 20 100 — Maximumhydrophobicretention

ReveRsed Phase ChRomatogRaPhy

tsK-geL® Reversed Phase ColumnsTosoh Bioscience offers a broad line of analytical and preparative TSK-GEL® columns for the Reversed Phase Chromatography (RPC) analysis of small molecular weight drugs, amino acids, carbohydrates, lipids, nucleic acid fragments, peptides and proteins. For high-speed separations we recommend the porous, silica-based TSK-GEL® Super Series and the nonporous, polymeric NPR columns. For greater stability at high pH or to benefit from alternative selectivities, we recommend either the silica-based 80TS columns or polymer-based TSK-GEL® columns. TSK-GEL® 80TS columns are stable up to pH 7.5. The best peak shapes for basic compounds and extra retention and selectivity for polar compounds are obtained with the recently introduced TSKgel ODS-100V and TSKgel ODS-100Z Reversed Phase Columns.

TSKgel Bonded Functional Particle Pore Exclusion Application/FeaturesColumn Phase Group Size(µm) Size(Å) Limit(Da)*

Octadecyl-2PW Monomeric C18 5 125 8,000 LowMWpeptidesandpharmaceuticalsunstableatlowpHOctadecyl-4PW Monomeric C18 7 500 200,000 MediumandhighMWpeptidesandproteinsespeciallyifunstable

atlowpHPhenyl-5PWRP Monomeric C6H5 10 1000 1,000,000 HighMWpeptidesandproteins.Phenylgroupmodifiesselectivity.Octadecyl-NPR Monomeric C18 2.5 Nonporous >1,000,000 RapidseparationofhighMWpeptidesandproteins

Properties of Polymer-based tsK-geL® RPC Columns

Properties of silica-based tsK-geL® RPC Columns

* for globular proteins

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Protein Column: TSKgelOctadecyl-NPRDimensions: 4.6mmx3.5cmPartNo.: CH0-7967MobilePhase: 10minlineargradientfrom15%to80% Acetonitrilein5mMHCIO4FlowRate: 1.5mL/minDetection: UV@220nmSample: A.Blankinjection, B.50ngeachof: 1.RibonucleaseA 4.Lysozyme 2.Insulin 5.Transferrin 3.Cytochromec 6.Myoglobin

Peptides Column: TSKgelOctadecyl-2PWDimensions: 4.6mmx15cmPartNo.: CH0-7968MobilePhase: 30minlineargradientfrom0.1%TFA/Acetonitrilefrom 90/10to30/70FlowRate: 1.0mL/minDetection: UV@215nmTemperature: AmbientSample: 50ngeachof: 1.Met-Enkephalin 5.Bombesin 2.Bradykinin 6.AngiotensinI 3.Leu-Enkephalin 7.Somatostatin 4.Neurotensin 8.Insulin(Bovine)

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Comparison of three Reversed Phase Columns Columns: A:TSKgelODS-80TS

B:TSKgelOctyl-80TS C:TSKgelCN-80TS

Dimensions: 4.6mmx15cmMobilePhase: 70%Methanol(exceptCN-80TSwith50%Methanol)FlowRate: 1.0mL/minTemperature: 25°CSample: 50ngeachof: 1.Uracil 3.Toluene 2.Benzene 4.Naphthalene

tsK-geL® Reversed Phase Columns (cont'd)

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tsK-geL® Reversed Phase Columns (cont'd)oRdeRing infoRmation

Super Series Part no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) Pricestainless steel columns I I I ICH0-7953 19541 Super-ODS,110Å 2.0 5.0 2

CH0-7954 19542 Super-ODS,110Å 2.0 10.0 2

CH0-7955 18154 Super-ODS,110Å 4.6 5.0 2

CH0-7956 18197 Super-ODS,110Å 4.6 10.0 2

CH0-7957 18275 Super-Octyl,110Å 4.6 5.0 2

CH0-7958 18276 Super-Octyl,110Å 4.6 10.0 2

CH0-7959 18277 Super-Phenyl,110Å 4.6 5.0 2

CH0-7960 18278 Super-Phenyl,110Å 4.6 10.0 2

CH0-7961 19672 Guardcartridge3/pk 2.0 1.0 2

CH0-8123* 19308 Guardcartridgeholder 2.0 —

CH0-7962** 18207 Guardcartridge3/pk 4.0 0.4 2

CH0-7963 18206 Cartridgeholder (for Part No. CH0-7962)

Oligo DNA and TMS Series

stainless steel columnsCH0-7964 13352 OligoDNARP,250Å 4.6 15.0 5

CH0-7965 13353 OligoDNARP,250Å 7.8 15.0 5

CH0-7966 07190 TMS-250,250Å 4.6 7.5 10

NPR and PW-RP Series

stainless steel columnsCH0-7967 14005 Octadecyl-NPRnonporous 4.6 3.5 2.5

CH0-7968 17500 Octadecyl-2PW,(100-200Å) 4.6 15.0 5.0

CH0-7969 17501 Octadecyl-2PW,(100-200Å) 6.0 15.0 5.0

CH0-7970 18754 Octadecyl-2PW,(100-200Å) 2.0 15.0 5.0

CH0-7971 13351 Octadecyl-4PW,500Å 4.6 15.0 7.0

CH0-7972 16257 Octadecyl-4PW,500Å 21.5 15.0 13.0

CH0-7973 18755 Octadecyl-4PW,500Å 2.0 15.0 7.0

CH0-7974 16258 Octadecyl-4PW,500Å 55.0 20.0 20.0

CH0-7975 18756 Phenyl-5PWRP,1000Å 2.0 7.5 10.0

CH0-7976 08043 Phenyl-5PWRP,1000Å 4.6 7.5 10.0

CH0-7977 16260 Phenyl-5PWRP,1000Å 21.5 15.0 13.0

CH0-7978 16261 Phenyl-5PWRP,1000Å 55.0 20.0 20.0

Guard column ProductsCH0-7979 42159 Phenyl-5PWRPCartridge,3/pk 2.0 1.0 —

CH0-7982† 16262 Phenyl-5PWRPGuardcolumn 45.0 5.0 —

CH0-7983 42161 Octadecyl-2PWCartridge 2.0 1.0 —

CH0-7984 17502 Octadecyl-2PWGuardcolumn 4.6 1.0 —

CH0-7985 17503 Octadecyl-2PWGuardcolumn 6.0 1.0 —

CH0-7986 42160 Octadecyl-4PWCartridge,3/pk 2.0 1.0 —

CH0-7987t 16749 Octadecyl-4PWPrepGuardgelKit —

CH0-7988† 16259 Octadecyl-4PWGuardColumn 45.0 5.0 —

Guard columnsCH0-8123* 19308 Guardcartridgeholder 2.0 — —

80TS Series Part no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) Pricestainless steel columns I I I ICH0-7922 18150 ODS-80TS,80Å 2.0 15.0 5

CH0-7923 18151 ODS-80TS,80Å 2.0 25.0 5

CH0-7924 17200 ODS-80TS,80Å 4.6 7.5 5

CH0-7925 17201 ODS-80TS,80Å 4.6 15.0 5

CH0-7926 17202 ODS-80TS,80Å 4.6 25.0 5

CH0-7927 16651 ODS-80TM,80Å 4.6 7.5 5

CH0-7928 08148 ODS-80TM,80Å 4.6 15.0 5

CH0-7929 08149 ODS-80TM,80Å 4.6 25.0 5

CH0-7930 14001 ODS-80TM,80Å 7.8 30.0 10

CH0-7931 17344 Octyl-80TS,80Å 4.6 15.0 5

CH0-7932 17345 Octyl-80TS,80Å 4.6 25.0 5

CH0-7933 17348 CN-80TS,80Å 4.6 15.0 5

CH0-7934 17349 CN-80TS,80Å 4.6 25.0 5

Guard column ProductsCH0-8124* 19325 ODS-80TSGuardcartridge,3/pk 2.0 1.0 5

AF0-7938 03410 Pre-columnfilter — — —

CH0-8123* 19308 Guardcartridgeholder 2.0 — —

ODS-100 Series

stainless steel columnsCH0-8147 21455 ODS-100V,100Å 4.6 15.0 5

CH0-8148 21456 ODS-100V,100Å 4.6 25.0 5

CH0-8149 21461 ODS-100Z,100Å 4.6 15.0 5

CH0-8150 21462 ODS-100Z,100Å 4.6 25.0 5

Guard column ProductsCH0-8152 21453 ODS-100VGuardcartridge,3/pk 3.2 1.5 5

CH0-8153 21454 ODS-100ZGuardcartridges,3/pk 3.2 1.5 5

CH0-8151 19018 Guardcartridgeholder — — —

ODS-120 Series

stainless steel columnsCH0-7939 07636 ODS-120A,120Å 4.6 15.0 5

CH0-7940 07124 ODS-120A,120Å 4.6 25.0 5

CH0-7941 07129 ODS-120A,120Å 7.8 30.0 10

CH0-7942 06172 ODS-120A,120Å 21.5 30.0 10

CH0-7943 18152 ODS-120T,120Å 2.0 15.0 5

CH0-7944 18153 ODS-120T,120Å 2.0 25.0 5

CH0-7945 07637 ODS-120T,120Å 4.6 15.0 5

CH0-7946 07125 ODS-120T,120Å 4.6 25.0 5

CH0-7947 07130 ODS-120T,120Å 7.8 30.0 10

CH0-7948 07134 ODS-120T,120Å 21.5 30.0 10

CH0-7949 07433 ODS-120T,120Å 55.0 30.0 15-25

CH0-7950 07434 ODS-120T,120Å 55.0 60.0 15-25

Guard column ProductsCH0-8124* 19325 ODS-120TGuardcartridge,3/pk 2.0 1.0 5

CH0-7951 07642 ODS-120T/AGuardcolumn 21.5 7.5 10

CH0-8123* 19308 Guardcartridgeholder 2.0 1.0 —

oRdeRing infoRmation

* For2.0mmIDcartridges**For4.6mmIDcolumnst For21.5mmIDcolumns† For55mmIDcolumns

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noRmaL Phase ChRomatogRaPhy

tsK-geL® normal Phase ColumnsSeparate hydrophilic compounds such as peptides, carbohydrates, and small polar molecules for drug discovery. The TSKgel Amide-80 column offers an excellent alternative to amino bonded stationary phases and consists of 5 or 10 µm silica particles with 80 Å pores in a stainless steel format.

Polyalcohols Column: TSKgelAmide-80Dimensions: 4.6mmx25cmPartNo.: CH0-7995MobilePhase: Acetonitrile/Water(75:25)FlowRate: 1.0mL/minTemperature: 25°CDetection: RISample: A.EthyleneGlycol10µm,20µL D.Xylitol10µm,20µL B.Glycerine10µm,20µL E.Mannitol10µm,20µL C.Erythritol10µm,20µL F.Inositol4µm,20µL

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Normal Phase Amide-80 SeriesPart no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) Pricestainless steel columns I I I I

CH0-7989 19694 Amide-80 2.0 5.0 5

CH0-7990 19695 Amide-80 2.0 10.0 5

CH0-7991 19696 Amide-80 2.0 15.0 5

CH0-7992 19697 Amide-80 2.0 25.0 5

CH0-7993 19532 Amide-80 4.6 5.0 5

CH0-7994 19533 Amide-80 4.6 10.0 5

CH0-7995 13071 Amide-80 4.6 25.0 5

CH0-7996 14459 Amide-80 7.8 30.0 10

CH0-7997 14460 Amide-80 21.5 30.0 10

Guard columnsCH0-7998 19021 Amide-80Guardcolumn 4.6 1.0 5

CH0-7999 14461 Amide-80Guardcolumn 21.5 7.5 10

CH0-8000 42140 Amide-80Guardcartridge,3/pk 2.0 1.0 5

CH0-8123 19308 Guardcartridgeholder — — (Forall2mmIDcolumns)


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Molecularweightofsample(Da)TSKgel Particle Pore Globular Dextrans PolyethyleneColumn Size(µm) Size(Å) proteins glycolsandoxides

SuperSW2000 4 125 5,000–1.5x105 1,000–3x104 500–15,000G2000SWXL/BioAssist®G2SWXL 5 125 5,000–1.5x105 1,000–3x104 500–15,000QC-PAKTSK200 5 125 5,000–1.5x105 1,000–3x104 500–15,000G2000SW 10,13 125 5,000–1x105 1,000–3x104 500–15,000

SuperSW3000 4 250 1x104–5x105 2,000–7x104 1,000–3.5x104

G3000SWXL/BioAssistG3SWXL 5 250 1x104–5x105 2,000–7x104 1,000–3.5x104

QC-PAKTSK300 5 250 1x104–5x105 2,000–7x104 1,000–3.5x104

G3000SW 10 250 2501x104–5x105 2,000–7x104 1,000–3.5x104

G4000SWXL/BioAssistG4SWXL 8 450 2x104–7x106 4,000–5x105 2,000–2.5x105

G4000SW 13,17 450 2x104–7x106 4,000–5x105 2,000–2.5x105

size exCLusion ChRomatogRaPhy - sW-tyPe

tsK-geL® sW-type• sW, sWxL, supersW

• silica-based for aqueous gfC of proteins and peptides

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Comparison of tsKgel supersW3000 and tsKgel g3000sWxL for the separation of Proteins Columns: A:TSKgelG3000SWXL

B:TSKgelSuperSW3000Dimensions: A:7.8mmx30cm B:4.6mmx30cmMobilePhase: 0.1MNa2SO4in0.1MPhosphateBufferwith0.05%NaN3,pH6.7FlowRate: A:1.0mL/min B:0.35mL/minTemperature: 25°CDetection: UV@220nmSample: 1.Thyroglobulin,0.5mg/mL(660,000Da) 4.RibonucleaseA,1.5mg/mL(12,600Da) 2.g-Globulin,1.0mg/mL(150,000Da) 5.p-AminobenzoicAcid,0.01mg/mL(137Da) 3.Ovalbumin,1.0mg/mL(43,000Da)

Properties and molecular Weight separation Ranges for tsK-geL® sW-type Packings

Data generated using the following conditions:

Columns: Two 4 µm, 4.6 mm x 30 cm TSK-GEL® SuperSW columns in series; two 5 µm, 7.8 mm x 30 cm TSK-GEL® SWXL columns in series; two 10 µm, 7.5 mm x 60 cm TSK-GEL® SW columns in series

Mobile Phases: Globular proteins: 0.3 M NaCl in 0.1 M (0.05 M for SWXL columns) phosphate buffer, pH 7 Dextrans and polyethylene glycols and oxides (PEOs): distilled water

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Elution volume (mL)

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TSK-GEL PW Columns TSK-GEL PWXL Columns

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Polyethylene Glycol and oxide calibration curves on TsK-Gel PW and TsK-Gel PWXl columns

tsKgel PW Columns Columns: A:TSK-GELPW A:G2000PW

B:G2500PW C:G3000PW D:G4000PW E:G5000PW F:G6000PW G:GMPWDimensions: All7.5mmx60cm B:TSK-GELPWXL

H:G2500PWXL

J:G3000PWXL K:G4000PWXL L:G5000PWXL M:G6000PWXL N:GMPWXL

Dimensions: All7.8mmx30cmMobilePhase: DistilledWaterFlowRate: 1.0mL/minDetection: RI

Molecularweightofsample(Da)

TSKgel Particle Average Polyethylene Dextrans** GlobularColumn Size*(µm) PoreSize(Å) glycolsandoxides proteins**

G1000PW 10 <100 upto1,000 — <2,000G2000PW 10,17,20 125 upto2,000 — <5,000G2500PWXL 6 <200 upto3,000 — <8,000G2500PW 10,17,20G3000PWXL 6 200 upto5x104 upto6x104 500–8x105

G3000PW 10,17,20G4000PWXL 10 500 2,000–3x104 1,000–7x105 1x104–1.5x106

G4000PW 17,22G5000PWXL 10 1000 4,000–1x106 5x104–2.5x106 <1x107

G5000PW 17,20,22G6000PWXL/BioAssistG6PWXL 13 >1000 4x104–8x106 5x105–5x107 <2x108

G6000PW 17,25GMPWXL 13 <100–1000 500–8x106 <5x107 <2x108

GMPW 17G-Oligo-PW 6 125 upto3,000 — <3,000G-DNA-PW 10 >1000 4x104–8x106 <5x107 <2x108

size exCLusion ChRomatogRaPhy - PW-tyPe

tsK-geL® PW-type• PW, PWxL

• Polymer-based for aqueous gfC of water soluble polymers

Properties and molecular Weight separation Ranges for tsK-geL® PW-type Packings

Data generated using the following conditions:

Columns: TSK-GEL® PW columns, 7.5 mm x 60 cm; TSKgel PWXL, G-Oligo-PW & G-DNA-PW, 7.8 mm x 30 cm

Mobile Phases: Polyethylene glycols and oxides: distilled water; dextrans and proteins: 0.2 M phosphate buffer, pH 6.8

Flow Rates: 1.0 mL/min

Note: * Larger particle sizes of each group are for 21.5 mm x 60 cm semi-preparative and 55 mm or 108 mm x 60 cm preparative columns. ** Maximum separation range determined from estimated exclusion limits.

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tsK-geL® sW- and PW-type (cont'd)

PW-Type

stainless steel columnscH0-8007 08031 G-Oligo-PW 7.8 30 6 14,000 0.5 – 0.8 1.0 40

CH0-8008 08032 G-DNA-PW 7.8 30 10 10,000 0.2 – 0.5 0.6 20

CH0-0748 08020 G2500PWXL 7.8 30 6 14,000 0.5 – 0.8 1.0 40

CH0-0752 08021 G3000PWXL 7.8 30 6 14,000 0.5 – 0.8 1.0 40

CH0-0756 08022 G4000PWXL 7.8 30 10 10,000 0.3 – 0.8 1.0 20

CH0-0760 08023 G5000PWXL 7.8 30 10 10,000 0.3 – 0.8 1.0 20

CH0-0764 08024 G6000PWXL 7.8 30 13 7,000 0.3 – 0.8 1.0 20

CH0-0767 08025 GMPWXL 7.8 30 13 7,000 0.3 – 0.8 1.0 20

CH0-0739 05760 G1000PW 7.5 30 10 5,000 0.5 – 1.0 1.2 20

CH0-0742 05761 G2000PW 7.5 30 10 5,000 0.5 – 1.0 1.2 20

CH0-0745 08028 G2500PW 7.5 30 10 5,000 0.5 – 1.0 1.2 20

CH0-0749 05762 G3000PW 7.5 30 10 5,000 0.5 – 1.0 1.2 20

CH0-0753 05763 G4000PW 7.5 30 17 3,000 0.5 – 1.0 1.2 10

CH0-0757 05764 G5000PW 7.5 30 17 3,000 0.5 – 1.0 1.2 10

CH0-0761 05765 G6000PW 7.5 30 17 3,000 0.5 – 1.0 1.2 10

CH0-8009 08026 GMPW 7.5 30 17 3,000 0.5 – 1.0 1.2 10

CH0-8010 05105 G2000PW 7.5 60 10 10,000 0.5 – 1.0 1.2 40

CH0-8011 08029 G2500PW 7.5 60 10 10,000 0.5 – 1.0 1.2 40

CH0-0750 05106 G3000PW 7.5 60 10 10,000 0.5 – 1.0 1.2 40

CH0-8012 05107 G4000PW 7.5 60 17 6,000 0.5 – 1.0 1.2 20

CH0-8013 05108 G5000PW 7.5 60 17 6,000 0.5 – 1.0 1.2 20

CH0-8014 05109 G6000PW 7.5 60 17 6,000 0.5 – 1.0 1.2 20

CH0-8015 08027 GMPW 7.5 60 17 6,000 0.5 – 1.0 1.2 20

PeeK columnsCH0-8016 20024 BioAssistG6PW 7.8 30 17 3,000 0.5 – 1.0 1.2 10

SW-Type Part no. Mfr. no. Description iD length Particle Min. number Flow Rate (ml/min) Maximum Price (mm) (cm) size (µm) Theoretical Range Max. Pressure Plates Drop (kg/ cm2)stainless steel columns I I I I I I I I

CH0-8001 18674 SuperSW2000 4.6 30 4 30,000 0.1 – 0.35 0.4 120

CH0-7439 18675 SuperSW3000 4.6 30 4 30,000 0.1 – 0.35 0.4 120

CH0-1251 08540 G2000SWXL 7.8 30 5 20,000 0.5 – 1.0 1.2 70

CH0-1252 08541 G3000SWXL 7.8 30 5 20,000 0.5 – 1.0 1.2 70

CH0-1253 08542 G4000SWXL 7.8 30 8 16,000 0.5 – 1.0 1.2 35

CH0-8002 16215 QC-PAKGFC200 7.8 15 5 10,000 0.5 – 1.0 1.2 40

CH0-8003 16049 QC-PAKGFC300 7.8 15 5 10,000 0.5 – 1.0 1.2 40

CH0-0708 05788 G2000SW 7.5 30 10 10,000 0.5 – 1.0 1.2 20

CH0-0712 05789 G3000SW 7.5 30 10 10,000 0.5 – 1.0 1.2 25

CH0-0716 05790 G4000SW 7.5 30 13 8,000 0.5 – 1.0 1.2 15

CH0-8004 05102 G2000SW 7.5 60 10 20,000 0.5 – 1.0 1.2 40

CH0-0713 05103 G3000SW 7.5 60 10 20,000 0.5 – 1.0 1.2 50

CH0-8005 05104 G4000SW 7.5 60 13 16,000 0.5 – 1.0 1.2 30

PeeK columnsCH0-8126 20027 BioAssistG2SWXL 7.8 30 5 20,000 0.5 – 1.0 1.2 70

CH0-8006 20026 BioAssistG3SWXL 7.8 30 5 20,000 0.5 – 1.0 1.2 70


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TSKgel Matrix* Particle Pore Functional Counter Exclusion Capacity SmallIon pKa ColumnColumn Size Size Group Ion Limit,PEG** (mgBSA/mL) capacity hard- (µm) (Å) (Da) (meq/mL) ware***

BioAssistS pMA 7,13 ~1300 Sulfopropyl Na+ ~4,000,000 70(1) 0.1 2.4 PEEKSP-5PW pMA 10,13,20 1000 Sulfopropyl Na+ 1,000,000 40(2) >0.1 2.3 S,GCM-5PW pMA 10,13 1000 Carboxymethyl Na+ 1,000,000 45(2) >0.3 4.2 S,GSP-2SW Silica 5 125 Sulfopropyl Na+ 10,000 ND 0.3 2.2 SSP-NPR pMA 2.5 ~0 Sulfopropyl Na+ 500 5(2) >0.1 2.3 SCM-2SW Silica 5 125 Carboxymethyl Na+ 10,000 110(2) >0.3 4.2 SCM-3SW Silica 10 250 Carboxymethyl Na+ 30,000 ND >0.3 4.2 SSCX PS-DVB 5 60 Sulfonicacid Na+,H+ — ND >1.5 — SOApak-A pMA 5 1000 Carbonicacid H+ — ND >1.5 — S

TSKgel Matrix* Particle Pore Functional Counter Exclusion Capacity SmallIon pKa ColumnColumn Size Size Group Ion Limit,PEG** (mgBSA/mL) capacity hard- (µm) (Å) (Da) (meq/mL) ware***

BioAssistQ pMA 10,13 ~4000 Polyamine Cl- >5,000,000 70 0.1 9.4 PEEKSuperQ-5PW pMA 10,13 1000 Trimethyl-amino Cl- 1,000,000 100 >0.15 12.2 S,GDEAE-5PW pMA 10,13,20 1000 DEAE Cl- 1,000,000 30 0.1 11.5 S,GDEAE-NPR pMA 2.5 ~0 DEAE Cl- 500 5 >0.15 11.2 SDNA-NPR pMA 2.5 ~0 DEAE ClO4

- 500 5 >0.15 11.2 SDEAE-2SW Silica 5 125 DEAE H2PO4

- 10,000 ND >0.3 11.2 SDEAE-3SW Silica 10 250 DEAE Cl- 30,000 ND >0.3 11.2 SQAE-2SW Silica 10 125 Trimethyl-amino H2PO4

- 30,000 ND >0.3 11.2 SSugarAXI PS-DVB 8 60 Trimethyl-amino HBO3

- — ND >1.2 — SSugarAXG PS-DVB 10 60 Trimethyl-amino HBO3

- — ND >1.2 — SSAX PS-DVB 5 60 Trimethyl-amino Cl- — ND >1.0 — S

ion exChange ChRomatogRaPhy

features and Benefits of ion exchange ColumnsBioassist®

• fewer runs to collect required sample amounts

• sharper peaks improve analysis and isolation

• Peak column housing reduces sample loss due to adsorption

• easy scale-up

• Polymethacrylate particles

Polymer-Based ion exchange• Chemically stable (ph 2-12)

• mechanically stable

• Withstands repeated cleaning with base, and use of organic solvents, denaturants and surfactants

• use same column for most biopolymers

• fast QC analysis and process monitoring

• Reduced solvent consumption and analysis time

silica-Based ion exchange• most suitable for analyzing smaller mW samples such as nucleotides, drug candidates, catecholamines and small peptides or proteins

Tosoh Bioscience offers a broad line of high efficiency columns for analysis and isolation of biomolecules by anion and cation exchange chromatography. In either mode of Ion Exchange Chromatography, the product line contains methacrylate-based as well as silica-based columns. Proteins, peptides, DNA and RNA derived oligonucleotides and other nucleic acid fragments are typical samples that are analyzed or isolated on TSK-GEL® ion exchange columns.

The methacrylate chemistry also forms the backbone of nonporous resin (NPR) columns such as DEAE-NPR, SP-NPR and DNA-NPR. These 4.6 mm ID columns are packed with 2.5 µm particles. High column efficiency coupled to low sample capacity restricts the application of these columns to fast analysis and micro-scale preparative isolation.

* pMA = poly methacrylate; PS-DVB = polystyrene-divinylbenzene (1) g-globulin; (2) hemoglobin

** Polyethylene glycol

*** PEEK = polyetheretherketone, S = stainless steel, G = glass

tsK-geL® Cation exchange Columns

tsK-geL® anion exchange Columns

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hemoglobin A1C

2 4 6 8 10 min0

oRdeRing infoRmationAnion Exchange Part no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) PricePeeK column I I I I I

CH0-8125 19685 BioAssistQ,4000Å 4.6 5.0 10

CH0-8017 21410 BioAssistQ,4000Å 10.0 10.0 13

stainless steel columns: Polymer-basedCH0-8018 13075 DEAE-NPR,nonporous 4.6 3.5 2.5

CH0-8019 18249 DNA-NPR,nonporous 4.6 7.5 2.5

CH0-8021 18757 DEAE-5PW,1000Å 2.0 7.5 10

CH0-0726 07164 DEAE-5PW,1000Å 7.5 7.5 10

CH0-8022 18257 SuperQ-5PW,1000Å 7.5 7.5 10

CH0-8023 08639 SugarAXI,60Å 4.6 15.0 8

CH0-8024 08640 SugarAXG,60Å 4.6 15.0 10

CH0-8025 07157 SAX 6.0 15.0 5

stainless steel columns: silica-basedCH0-8026 07166 QAE-2SW,125Å 4.6 25.0 5

CH0-8027 07168 DEAE-2SW,125Å 4.6 25.0 5

CH0-8028 07163 DEAE-3SW,250Å 7.5 7.5 10

Guard column ProductsCH0-8029 17088 DEAE-NPRGuardcolumn 4.6 0.5 5

CH0-8030 18253 DNA-NPRGuardcolumn 4.6 0.5 5

CH0-8031 18388 SuperQ-5PWGuardgelKit — — 20

CH0-8032 07210 DEAE-5PWGuardgelKit — — 20

CH0-8033 42152 DEAE-5PWGuardcartridge 2.0 1.0 10

CH0-8034 07648 DEAE-SWGuardgelKit — — 20

CH0-8123 19308 Guardcartridgeholder 2.0 1.5 —

oRdeRing infoRmationCation ExchangePart no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) PricePeeK column I I I I I

CH0-8035 19686 BioAssistS,1300Å 4.6 5.0 7

CH0-8036 21411 BioAssistS,1300Å 10.0 10.0 13

stainless steel columns: Polymer-basedCH0-8037 13068 CM-5PW,1000Å 7.5 7.5 10

CH0-8038 18758 SP-5PW,1000Å 2.0 7.5 10

CH0-0728 07161 SP-5PW,1000Å 7.5 7.5 10

CH0-8039 13076 SP-NPR,nonporous 4.6 3.5 2.5

CH0-8040 07156 SCX(Na+) 6.0 15.0 5

CH0-8041 07158 SCX(H+) 7.8 30.0 5

CH0-8042 16653 OApak-A,1000Å 7.8 30.0 5

stainless steel columns: silica-basedCH0-8043 07165 SP-2SW,125Å 4.6 25.0 5

CH0-8044 07167 CM-2SW,125Å 4.6 25.0 5

CH0-8045 07162 CM-3SW,250Å 7.5 7.5 10

Guard column ProductsCH0-8046 13069 CM-5PWGuardgelKit — — 20

CH0-8047 16094 CM-5PWPrepGuardgelKit — — 20

CH0-8048 07211 SP-5PWGuardgelKit — — 20

CH0-8049 42153 SP-5PWGuardcartridge 2.0 1.0 10

CH0-8050 08807 SP-5PWGuardgelKit,Glass — — 20

CH0-8051 14467 SP-5PWGuardcolumn,Glass 20.0 2.0 13

CH0-8052 16093 SP-5PWPrepGuardgelKit — — 20

CH0-8053 07932 SP-5PWGuardcolumn 45.0 5.0 20

CH0-8054 07650 CM-SWGuardgelKit — — 20

CH0-8055 16654 OApak-PGuardcolumn 6.0 4.0 10

CH0-8123 19308 Guardcartridgeholder 2.0 1.5 —

nucleotides Column: TSKgelDEAE-2SWDimensions: 4.6mmx25cmPartNo.: CHO-8027MobilePhase: A:Acetonitrilein0.1Mphosphate,pH3.0,20/80 B:Acetonitrilein0.5Mphosphate,pH3.0,20/80Gradient: 30minlineargradientfrombufferAtoBFlowRate: 1.0mL/minDetection: UV@260nmSample: HemoglobinStandard 1.AMP 4.ADP 2.IMP 5.ATP 3.GMP

hemoglobin a1C Column: TSKgelSP-NPRDimensions: 4.6mmx3.5cmPartNo.: CHO-8039MobilePhase: A:0.02MMES,and0.02MHEPES-NaOH,pH6.0 B:0.02MMES,and0.02MHEPES-NaOH,pH8.0Gradient: 10minlineargradientfrom32%to75%bufferB (pH6.66topH7.43)FlowRate: 1.5mL/minDetection: VIS@415nmSample: HemoglobinStandard

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Column: TSKgelPhenyl-5PWDimensions: 7.5mmx7.5cmPartNo.: CH0-8061MobilePhase: 60minlineargradientof(NH4)2SO4from1.5Mto 0.0Min0.1Mphosphatebuffer(pH7.0)FlowRate: 0.5mL/minTemperature: 25°CDetection: UV@280nmSample: 1.Myoglobin 2.RibonucleaseA 3.Ovalbumin 4.a-chymotrypsin;concentration:0.025%to1.6%

oRdeRing infoRmationHydrophobic InteractionPart no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) Pricestainless steel columns I I I I I

CH0-8056 18760 Ether-5PW,1000Å 2.0 7.5 10.0

CH0-8057 08641 Ether-5PW,1000Å 7.5 7.5 10.0

CH0-8058 08642 Ether-5PW,1000Å 21.5 15.0 13.0

CH0-8059 16255 Ether-5PW,1000Å 55.0 20.0 20.0

CH0-8060 18759 Phenyl-5PW,1000Å 2.0 7.5 10.0

CH0-8061 07573 Phenyl-5PW,1000Å 7.5 7.5 10.0

CH0-8062 07656 Phenyl-5PW,1000Å 21.5 15.0 13.0

CH0-8063 07938 Phenyl-5PW,1000Å 55.0 20.0 20.0

CH0-8064 14947 Butyl-NPR,nonporous 4.6 3.5 2.5

PeeK columnsCH0-8065 20023 BioAssistPhenyl-5PW, 7.8 5 10.0 1000Å

Guard column ProductsCH0-8066 42156 Ether-5PWGuardcartridge 2.0 1.0 10.0

CH0-8067t 08643 Ether-5PWGuardgelKit – – 20.0

CH0-8068 42155 Phenyl-5PWGuardcartridge 2.0 1.0 10.0

CH0-8069** 07652 Phenyl-5PWGuardgelKit – – 20.0

CH0-8070† 07936 Phenyl-5PWGuardcolumn 45.0 5.0 20.0

CH0-8123* 19308 Guardcartridgeholder 2.0 – –

hydRoPhoBiC inteRaCtion ChRomatogRaPhy - hiC

tsK-geL® hydrophobic interaction Columns• tsKgel ether-5PW, tsKgel Phenyl-5PW, tsKgel Butyl-nPR

• tsKgel ether-5PW and Phenyl-5PW columns now available in a 2 mm id format

• Large 1000 Å pore size of the base matrix accommodates proteins up to 5 x 106 da

• Polymeric resin is chemically and physically stable to changes in ph and ionic strength

• Compatible with a variety of organic solvents

Hydrophobic Interaction Chromatography (HIC) is a separation technique based on the interaction between hydrophobic groups on a protein and a hydrophobic ligand on the solid support. HIC offers a distinct advantage for unstable proteins: it can be run using moderate concentrations of ammonium sulfate, which favors the stability of many proteins.

Benefits of hiC • hiC adds flexibility during method development as an alternative to ion exchange Chromatography

• Wide ph range (2-12) of the base resin enables robust cleaning options

• seamless scalability from analytical to preparative scale

• eliminates undesirable interactions with column hardware

Sample Loading (mg)

Pea

k W

idth

(mL)

4

2

1

3

0.1 1.01.0

1.5

2.0

2.5

Dependence of Peak Width on sample loading in the separation of Proteins

* For2.0mmIDcartridges

** For7.5mmIDcolumnst For21.5mmIDcolumns † For55mmIDcolumns

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ColumnPacking LigandType LigandConcentration AdsorptionCapacity Sample

ABA-5PW p-aminobenzamidine notavailable 3-4mg/mL-resin trypsinBoronate-5PW m-aminophenylboronate notavailable 40µmole/mL-resin sorbitolChelate-5PW iminodiaceticacid 20µmole/mL-resin notavailable notavailableTresyl-5PW tresyl ca.20µmole/mL-resin >60mg/gdryresin soybean (couplingcapacity) trypsininhibitor

affinity ChRomatogRaPhy

tsK-geL® affinity Columns• tsKgel aBa-5PW, tsKgel Boronate-5PW,

tsKgel Chelate-5PW, tsKgel tresyl-5PW

• enhanced access of large proteins to affinity ligands

• high efficiency and wide ph range (2-12)

The Tosoh Bioscience TSK-GEL Affinity Chromatography (AFC) column line consists of three group-specific stationary phases and one activated packing material called Tresyl-5PW. Affinity chromatography offers the highest level of specificity and selectivity in biomolecular separations and purifications.

oRdeRing infoRmationAffinityPart no. Mfr. no. Description iD length Particle (mm) (cm) size (µm) Pricestainless steel columns I I I I I

CH0-8071 13067 ABA-5PW,1000Å 7.5 7.5 10

CH0-8072 13066 Boronate-5PW,1000Å 7.5 7.5 10

CH0-8073 08645 Chelate-5PW,1000Å 7.5 7.5 10

CH0-8074 14456 Tresyl-5PW,1000Å 7.5 7.5 10

PeeK columnsCH0-8075 20022 BioAssistChelate,1000Å 7.8 5 10

Guard column ProductsCH0-8076 13127 ABA-5PWGuardgelKit — — —

CH0-8077 13125 Boronate-5PWGuardgelKit — — —

CH0-8078 08647 Chelate-5PWGuardgelKit — — —

Bulk PackingCH0-8079 16208 Tresyl-5PW,2gdrygel* — — 10

* 1 g is approximately 3.5 mL

Characteristics of tsK-geL® afC Columns

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toyosCReen®

Process method development ColumnsHistorically, resin screening was accomplished by packing various bulk resins into small columns by hand, requiring significant investments in time and cost. In order to improve the efficiency of resin screening experiments, pre-packed ToyoScreen columns, containing the most popular Toyopearl® resins, now provide a low cost, convenient way to evaluate different Toyopearl ligands. The ToyoScreen Series are for ion exchange, hydrophobic interaction and affinity chromatography. The system consists of a set of six 1 mL or 5 mL cartridge columns to be mounted in a holder. The columns exhibit reproducibility and show high numbers of theoretical plates. The columns are compatible to most common HPLC and FPLC® systems and are reusable, as is the holder.

oRdeRing infoRmationPart no. Mfr. no. Description Unit Priceion exchange I I I

CH0-8080 21360 ToyoScreenDEAE-650M 1mLx6each

CH0-8081 21361 ToyoScreenDEAE-650M 5mLx6each

CH0-8082 21362 ToyoScreenSuperQ-650M 1mLx6each

CH0-8083 21363 ToyoScreenSuperQ-650M 5mLx6each

CH0-8084 21364 ToyoScreenQAE-550C 1mLx6each

CH0-8085 21365 ToyoScreenQAE-550C 5mLx6each

CH0-8086 21366 ToyoScreenCM-650M 1mLx6each

CH0-8087 21367 ToyoScreenCM-650M 5mLx6each

CH0-8088 21368 ToyoScreenSP-650M 1mLx6each

CH0-8089 21369 ToyoScreenSP-650M 5mLx6each

CH0-8090 21370 ToyoScreenSP-550C 1mLx6each

CH0-8091 21371 ToyoScreenSP-550C 5mLx6each

hydrophobic interactionCH0-8092 21372 ToyoScreenEther-650M 1mLx6each

CH0-8093 21373 ToyoScreenEther-650M 5mLx6each

CH0-8094 21374 ToyoScreenPhenyl-650M 1mLx6each

CH0-8095 21375 ToyoScreenPhenyl-650M 5mLx6each

CH0-8096 21376 ToyoScreenButyl-650M 1mLx6each

CH0-8097 21377 ToyoScreenButyl-650M 5mLx6each

CH0-8098 21378 ToyoScreenHexyl-650C 1mLx6each

CH0-8099 21379 ToyoScreenHexyl-650C 5mLx6each

CH0-8100 21380 ToyoScreenPPG-600M 1mLx6each

CH0-8101 21381 ToyoScreenPPG-600M 5mLx6each

CH0-8102 21382 ToyoScreenSuperButyl-550C 1mLx6each

CH0-8103 21383 ToyoScreenSuperButyl-550C 5mLx6each

oRdeRing infoRmationPart no. Mfr. no. Description Unit PriceAffinity I I I

CH0-8104 21384 ToyoScreenAF-Chelate-650M 1mLx6each

CH0-8105 21385 ToyoScreenAF-Chelate-650M 5mLx6each

CH0-8106 21386 ToyoScreenAF-BlueHC-650M 1mLx6each

CH0-8107 21387 ToyoScreenAF-BlueHC-650M 5mLx6each

CH0-8108 21388 ToyoScreenAF-Red-650M 1mLx6each

CH0-8109 21389 ToyoScreenAF-Red-650M 5mLx6each

Mixed PacksCH0-8110 21392 ToyoScreenIECAnion-650M 1mLx3Grades x2each

CH0-8111 21393 ToyoScreenIECAnion-650M 5mLx3Grades x2each

CH0-8112 21394 ToyoScreenIECCation-650M 1mLx3Grades x2each

CH0-8113 21395 ToyoScreenIECCation-650M 5mLx3Grades x2each

CH0-8114 21396 ToyoScreenIEC-650M 1mLx6Grades x1each

CH0-8115 21397 ToyoScreenIEC-650M 5mLx6Grades x1each

CH0-8116 21398 ToyoScreenHIC-650M 1mLx6Grades x1each

CH0-8117 21399 ToyoScreenHIC-650M 5mLx6Grades x1each

Toyoscreen column AccessoriesCH0-8118 21400 ToyoScreenColumnHolder ea

CH0-8119 42194 ToyoScreenHolderw/Fittings 2 IncludesMfr.Nos.21400, 42195,and42196

CH0-8120 42195 ColumntoColumnConnector ea

AQ0-8121 42196 M6Adaptor ea

ToyoScreen®, TSK-GEL®, TSKgel®, Tosoh Bioscience®, Toyopearl MegaCap® and BioAssist® are registered trademarks of Tosoh Corporation, the parent company of Tosoh Bioscience.

Tosoh Bioscience llc - Phenomenex · 2008. 10. 16. · CH0-7951 07642 ODS-120T/A Guard column 21.5 7.5 10 CH0-8123* 19308 Guard cartridge holder 2.0 1.0 — oRdeRing infoRmation *

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