TL III Common Bean Breeding Improvement Plans of Ethiopia Berhanu Amsalu (PhD) EIAR, Melkassa ARC, Presented at TL III Genetic Gains Workshop 1 st –2 nd September 2016 Jacaranda Hotel, Nairobi, Kenya
TL III Common Bean Breeding Improvement
Plans of Ethiopia
Berhanu Amsalu (PhD)
EIAR, Melkassa ARC,
Presented at TL III Genetic Gains Workshop
1st–2nd September 2016
Jacaranda Hotel, Nairobi, Kenya
Doc ID
1
Aim: To ensure sustainable improvement of common bean
productivity & production through development and utilization
of marketable, stable, high yielding varieties which have
tolerance /resistance to major stresses with improved crop
management packages and there by contribute to food
security and income generation.
Breeding objectives
Doc ID
2
1. Variety generation using classical breeding approach
2. Introduce & adapt new market oriented (demand driven)
bean varieties for fast-track generation of varieties
3. Participatory varietal selection (PVS)
Varietal generation methods
Main target of varietal development
1. Development of beans for local market & consumption
(red, red mottled, cream/yellow market class beans)
2. Development of beans for export market (canning type
bean development) (white, speckled, red kidney beans)
Doc ID
3
These aim of breeding again will be refined based on seed size
(small, medium & large bean market class)
Breeding for abiotic stress
drought (drought tolerant, early variety development)
low soil fertility tolerant
acid soil tolerant variety development
Breeding for biotic stress
Disease resistant (CBB, HB, rust …)
Insect pest resistant (bruchid)
Breeding objectives
Doc ID
4
Breeding program pipeline
under progress
Doc ID
5
Widening Genetic pool
Collection of bean landraces across the
country
Crossing/hybridization
Introduction of lines from CIAT/PABRA
targeted for different constraints & quality
Doc ID
6
Crossing/hybridization of beans
1. Seed characteristics
(size, color, brilliance)
2. Abiotic stress
Drought tolerance
3. Disease resistance (CBB)
Aim of Crossing
Future plan
4. Nutritional quality (Fe & Zn, protein) , cooking time,
5. Low soil fertility & acid soil
Doc ID
7
Breeding program pipeline
Product target 1: Large white bean development (100% for market)
Double crosses
Canpsula/SAB 626//Canpsula/SAB 629……..140 F2 derived F5
families
Ranjonomby/SAB 626//Ranjonomby/SAB 629 ….88 F2 derived
F5 families
Single crosses
Canpsula/SAB 713……124 F2 derived F6 families
Total population: 352
Two PNVT has been set from the selected
lines from above population after evaluation
at nursery
Doc ID
8
Breeding program pipeline cont’d
Product target 2: Large rad bean development (75%
export market & 25% local market/consumption)
Double crosses
DRK/SAB 626//DRK/SAB 629-----156 F2 derived F4
families
Product target 3: Small white bean development
(100% for export market/for canning)
Single cross
G4445/G11239 (small white)…… 816 F4 derived F7
lines
One pre-national variety trial has been set
Doc ID
9
Breeding program pipeline cont’d
Product target 4: Small red bean development for improvement for
drought tolerance (100% for local market & consumption) now export
market emerged
Single crosses
Dicta 105/SEA 5……..150 F2 derived F6 families
Dicta 105/SER 16…….74 F2 derived F6 families
Dicta 105/BAT 477…..33 F2 derived F6 families
Total population: 257
Doc ID
10
Product target 5: CCB resistance small white bean development
oG 4445/VAX 3 …….425 F4 derived F7 lines
oPAN 182/VAX 3……213 F4 derived F7 lines
Total population : 638
Doc ID
11
Advancing the breeding lines
We have advanced some lines from segregating
population:
1. Three Pre-National performance trials (NPT)
2. Three Nurseries
3. Two nurseries (small white & small red) trials has
been formulate and sent for two regional research
sites
Doc ID
12
Implementing marker assisted
selection
Application of MAS
1. Bruchid resistance crosses for 1700 F3
population
(for small red & white pea bean population)
PhD student
2. CBB resistance populations >600 lines
Doc ID
13
Utilization of advanced data base
management system
Doc ID
14
Application of BMS
1 National PT 10
2 Pre-National PT 3
3 Nursery 4
Total 17
Germplasm lists, nurseries, trials, field book
Doc ID
15
Facility building by TL III
Toyota Hilux Double Cabin
Purchased & delivered
Automated Mattson
cooker
to be purchased soon
Doc ID
16
Complimentary funds and synergy with TL III
project to enhance bean breeding
modernization and to enhance genetic gain
plans
(four years project)
Doc ID
17
Breeding Modernization project EIAR_BMGF
Ethiopia EIAR
Doc ID
18
I. Bean products concept
1. Large white bean for export market with drought and disease
tolerance
2. Large red bean with drought and disease tolerance (75% export; 25%
domestic)
3. Small white bean for export market with drought and disease tolerance
4. Small red bean with drought and disease tolerance
5. Large speckled bean for export market with drought and disease
tolerance
6. Large red mottled bean with drought and disease tolerance
Doc ID
19
II. Automation & mechanisation
Seed related equipment
Digital seed counter
Small digital balance
Large digital balance Seed cleaner
Seed treatment machine Moisture meter bar-code readers packet printer Germination cabinet
Field facilities Cold store Seed store
Glass house Controlled irrigation
Field Equipment Plot thresher
Single plant thresher Tractor for field work Plot planter
Laboratory Flame photometer
Canning equipment Autoclave Freezer (-20C)
Doc ID
20
III. Support services
Quality
Determine best way to measure protein, Fe and Zn content
Determine best canning equipment required
Pathology
Develop high throughput screening assays compatible with pipeline:
Markers
Nominate a set of up to ~200 key lines for a
preliminary diversity analysis from each
agro-ecology
Doc ID
21
Promotion, Progression,
Release Criteria and Procedures
Advanced Trials
Release
Example criteria:
• Yield > current accepted
variety
• Disease resistance >
current accepted variety
• Quality equivalent to best
current variety
• Reliability >= current
accepted variety
Weightings for criteria
depend on the market forces
and the customers
preferences
National variety trial year 1
National variety trial year 2
IV . Product advancement scheme
Doc ID
22
Interventions to increase
genetic gain
Examples of ways modern plan breeding programs improve
genetic gain
1. New statistical analysis methods
2. Enhanced breeding program efficiency (IT
mechanization etc)
3. Alternative Phenotyping Methods (Markers, NIR)
Doc ID
23
Crossing handling
Field phenotyping
Multi-location trial management up to DUS (release)
Lines introduced from CIAT (TL III products) as an
input in the system to the target product
etc …..
Role of TL III in the EIAR_BMGF
project
Doc ID
24
Alignment & synergy of TL III with
Integrated Genotyping Service & Support (IGSS)
project plans
(four years project)
Ethiopia EIAR
Doc ID
25
Planned Activities
1 Pre-breeding activities
Identification of type and number of
traits of interest
Identification & collection of key
germplasms for genome profiling
Genome profiling
Doc ID
26
Activity 2: Implementation of
cultivar development
Development of customized
cultivar development pipelines
Population development
Field screening
Genotyping
Data analysis
TL III
Crossing handling
Field phenotyping
Multi-location trial
management up to
DUS (release)
Doc ID
27
Activity 3 Capacity building/Training
Molecular breeding,
Bioinformatics etc
Doc ID
28
Human capacity Development
Current human resource
1PhD, 1MSc, 2BSc and 6 technicians
Multi-disciplinary team (crop protection (2), Scio-economics
(2), mechanization (1), food scientist (1), soil scientist (1) ,
seed science (1)
Study
PhD : Three under study and one will return early next year
MSc: Two under study and one will return within three month
One BSc: will join MSc after one/two month
Haring new staff plan
Two BSc
One MSc
Doc ID
29
Acknowledgement Ethiopia EIAR
Doc ID
30
Thank you!