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Title Page

CZECH DNA PROJECT:

BACKGROUND and UPDATE

ACKNOWLEDGEMENTS

* Dr. Bryan Sykes

* Dr. Gary Kocurek

OUTLINE

• DNA Testing Uses

• Mitochondrial DNA

• Y Chromosome DNA

• Autosomal DNA

• Czech DNA Project

• Moravian Valach Paper

• Helpful Hints

DNA Testing Uses

• “Normal Genealogy”-connecting family

branches

• “ Deep Ancestry”

* Prehistory

* Deep time depth

DNA Testing Uses(cont)

• Determine if two people are related and

descended from the same ancestor

• Confirm your family tree

• Find out if others with your surname are

related

• Confirm surname variants

• Find others to whom you are related

DNA TESTING COMPANIES

• Family Tree DNA

http://www.familytreedna.com

• 23 and me

http://www.23andme.com

• Ancestry.com

http://dna.ancestry.com

PASSENGER LIST PRIMARY

SOURCES-Not USA

MITOCHONDRIAL DNA

• Passed from mother to children

• 16,569 bases

• 33 haplogroups

• Haplogroups are continent specific

• Seven are specific to Europe

• 95% of all Europeans

• Haplogroup origins-10,000-45,000 years ago

mtDNA STUDY RESULTS

Haplogroup Dr. Sykes Czech DNA Project

H 47% 41.0%

J 17% 9.0%

U 11% 14.1%

T 9% 11.6%

K 6% 9.3%

X/W/I 6% 7.3%

V 4% 3.4%

Other-L2 0% .3% (Tuareg)

N 0% 1.7% (Jewish?)

C 0% .3% (Central Asia)

Total 100% 100.0%

Y-Chromosome DNA

• Passed from father to son

• 58 million bases

• 90% nonrecombining

Y Testing

• Several different techniques

• Short tandem repeats(STRs)

• Commercially available-9-111 markers

• Single nucleotide polymorphisms (SNPs)

• Full Genomes-$1250

• Big Y-FTDNA-10 million bases-$495

Y CHROMOSOME TEST RESULTS

Haplogroup Czech DNA Project

R1b 29.4%

R1a 26.7%

I1/2b 17.7%

G/G2 6.8%

J1/2 5.6%

E1b 4.9%

I2a 4.3%

N 3.2%

F/C3 .4% (Central Asia)

T 1.0%

Total 100.0%

AUTOSOMAL DNA

• 22 of 23 pairs of chromosomes are called “autosomes”. The 23rd pair determines sex.

• Inherited from both parents

• Nearly complete genetic record

• Recombination

• DNA signal drops rapidly with distance of relationship

• FTDNA, 23 and Me, and Ancestry provide autosomal DNA testing

CZECH DNA PROJECT

• Website:

http://www.familytreedna.com/public/Czech

• Over 600 participants

• For testing, contact:

Leo Baca: [email protected]

Joan Hudson: [email protected]

12 marker Y chromosome test: $49

mtDNA Plus test(HVR1 and 2): $49

Family Finder Test (autosomal DNA): $99

MORAVIAN VALACH PAPER

• “Y-chromosomal diversity of the Valachs

from the Czech Republic: Model for

isolated population in Central Europe”

• Croation Medical Journal, 2011.

• Authors: Edvard Ehler, Daniel Vanek,

Vlastimil Stenzel, Vaclav Vancata

CONCLUSIONS

• Moravian Valachs resemble more the

isolated Balkan populations such as the

Aromuns.

• Moravian Valachs represent a unique

Central European population model for

population genetics.

• Valach modal haplotype was not detected in

the Czech population.

VALACH MODAL HAPLOTYPE

• DYS 390= 24

• DYS 391= 10

• DYS 392= 11

• DYS 393= 13

• DYS 385a= 11

• DYS 385b= 14

• DYS 437= 15

• DYS 439= 12

OTHER ACTIVITIES

• Connecting surname branches

Roeder/Roder

• Disproved genetic connections

Krenek/Krenik/Krajnik

Uhlik branches

Kaspar branches

Baca branches

OTHER ACTIVITIES

• Investigating haplotype matches between

different surnames

Hrouda, Kopecky, Beznoska

Prohaska, Dvorak, Riha

Bartos, Korican, Dolezel

Janca, Samz, Vosoba

Smisek, Prokop, Hollas

LIST GROUP

• [email protected]

• Dedicated to the use of genetics in genealogy

SUGGESTED READINGS

• Steve Olson. “Mapping Human History-Discovering the

Past Through Our Genes.” 2002.

• Stephen Oppenheimer. “The Real Eve-Modern Man’s Journey Out of Africa.” 2003.

• Chris Pomeroy. “DNA and Family History.” 2004.

• John H. Relethford. “Reflections of Our Past-How Human History is Revealed in Our Genes.” 2003.

• Colin Renfrew & Katie Boyle. “Archaeogenetics: DNA and the Population Prehistory of Europe.” 2000.

• Megan Smolenyak & Ann Turner. “Trace Your Roots with DNA.” 2004

SUGGESTED READINGS

• Bryan Sykes. “The Seven Daughters of

Eve.” 2001.

• Spencer Wells. “The Journey of Man-A

Genetic Odyssey.” 2002.