In vivo determination of the hypoglycemic effect of Mollugo oppositofollia Linn. (Molluginaceae) plant extracts in Alloxan- induced male Sprague- Dawley rats HERNANDEZ, RAY CONRAD. , JAVIER, JEZREEL ROLLAN., LIM, ALEC JACOB, MANUEL, ROSELLE, PENSOTES, DARRYL VAN , RAMORAN, EDRICK GLENN DR. GAVINO IVAN TANODRA University of Santo Tomas Faculty of Pharmacy 1
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In vivo determination of the hypoglycemic effect of Mollugo oppositofollia Linn. (Molluginaceae) plant extracts in Alloxan-induced male Sprague-Dawley ratsHERNANDEZ, RAY CONRAD. , JAVIER, JEZREEL ROLLAN., LIM, ALEC JACOB, MANUEL, ROSELLE, PENSOTES, DARRYL VAN , RAMORAN, EDRICK GLENN
DR. GAVINO IVAN TANODRA
University of Santo TomasFaculty of Pharmacy
2
Introduction
3Diabetes Mellitus
Disorder in which blood sugar (glucose) levels are abnormally high.
Metabolic diseases characterized by hyperglycemia Defects in the B-cells of the pancreas
(American Diabetes Association, 2003)
4Type 1 Diabetes
insulin-dependent diabetes juvenile-onset diabetesautoimmune destruction of the β-cells of
the pancreas
5Insulin
hormone released from the β-cells of pancreas controls the amount of glucose in the blood
6Prevalence of Diabetes According to the International Diabetes
Federation & World Health Organization 387 million people around the world have diabetes cause of 1.5 million deaths and is projected to rise
by more than 50% in the next 10 years 7th leading cause of death in the world
7Alloxan 2,4,5,6- pyrimidinetetrone diabetogenic agent induce Type 1 diabetes in experimental
animals administered parenterally
intravenously intraperitoneally subcutaneously.
Usual dose is 65 mg/kg
8Mollugo oppositofollia Linn. : Botanical Description
Kingdom: Plantae
Phylum: Tracheophyta
Class: Magnoliospida
Order: Caryophyllales
Family: Molluginaceae
Genus: Mollugo
Species: Mollugo oppositofolia
9Mollugo oppositofollia Linn. : Botanical Description
Characterized by diffuse prostrate, annual herb
Made up of numerous, dichotomously branched slender stems.
Possesses 13-22 mm long leaves, in whorls of 4-5, unequal, and oblanceolate
Consists of small, white flowers, in axillary fascicles of 2 or more, with long filiform pedicels
applied topically for the treatment of skin diseases and itch
used with castor oil as treatment for ear ache natural source of antioxidant
Mollugo oppositofollia Linn.
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12
Problem Rationale
13Objectives
To determine which fraction will elicit the highest hypoglycemic activity.
To identify the constituents present in the different fractions.
14Scope and Limitations Determination of efficacy by decreasing blood
glucose level using a glucometer Toxicity will not be performed Lingayen Male Sprague-dawley rats will be used Diabetes induction by use of Alloxan Soxhlet
15Significance of the Study
To serve as a tool for application of what the researchers learned in the field of pharmacy.
To serve as a reference for future hypoglycemic studies.
To provide information about the hypoglycemic effect of Mollugo oppositifolia Linn.
Best?
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Research Design
17
Collection of Mollugo oppositifolia Linn. plant sample
Research Design
Identification of the wholeplant sample
Drying of the plant sample
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Grinding of the plant sample
Extraction with hexane, chloroform, 2-butanol, methanol
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Rotary Evaporator
Soxhlet Apparatus
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Phytochemical Analysis
IACUC Application
Induction of diabetes to male Sprague- Dawley rats
21Group I: Normal healthy control animals
(fed with normal chow and water)Group II: Diabetes induced control
animals treated with alloxan monohydrate in sterile saline (150 mg/kg by i.p. injection, single dose)
Group III: Diabetes induced animal treated with hexane extract (500 mg/kg/day orally for 35 days)
22Group IV: Diabetes induced animal
treated with chloroform extract (500 mg/kg/day orally for 35 days)
Group V: Diabetes induced animal treated with 2-butanol extract (500 mg/kg/day orally for 35 days)
Group VI: Diabetes induced animal treated with methanol extract (500 mg/kg/day orally for 35 days)
Group VII: Drug control-animals, diabetes induced rats treated with Insulin (1 U/200 g /day intraperitonially for 35 days)
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Administration of crude extracts
Biochemical Analysis
Statistical Analysis
Glucometer
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25REFERENCESThe Wonders of ‘Guyabano’ and Other Philippine Medicinal Plants Documented in New
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