Thesis Presentation 30-09-11

8/3/2019 Thesis Presentation 30-09-11

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Preformulation ofAntifungal Cream from

the Essential Oilextracted byHydrodistillation of the

Rhizomes of LuyangDilaw (Curcuma longaLinn.)

Mapaye, Ma Rona Louise C., Prim, Racelly

Ena P.,Ramos, Cherish Ivy DG., Ramos, CrystalGail H.,

Vicarez, Roy G.


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INTRODUCTIONThe rhizome ofCurcuma longa Linn., which is also known

as turmeric (English) or luyang dilaw(Filipino), has been widely used medicinally and as a

source ofspice.


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STATEMENT OF THEPROBLEM

This shows the sequence ofmethods required in establishing apreformulation profile for the

cream.

1. How much essential oil will 16 kg of luyang dilaw yield usinghydrodistillation?

2. What will be the preformulation profile of the essential oil ofluyang dilaw?

3. Would the essential oil from the rhizomes of luyang dilaw exhibitan antifungal effect?

4. What would be the minimum inhibitory concentration of the oil?

5. Would the essential oil pass the microbial limit test?6. Would the formulated cream from the rhizomes of lu an dilaw


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SIGNIFICANCE OF THESTUDY

By testing the extracted turmeric oil,

investigation and verification upon itsbeneficial effects are established.

Testing the cream for its safetyand efficacy as an antifungalcream.


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SCOPE AND LIMITATIONS

The source of the Luyang Dilaw is from

Nueva Ecijaonly.

The study has certain limitations that areneeded to be considered due to differentfactors that affects it.

> Availability ofequipments & reagents

> Time

> Financing


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CONCEPTUAL FRAMEWORK


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METHODOLOGY

Plant Identification

Plant Collection

Plant Extraction

Physical

Tests

Microbial

Limit Test

Compatibi

lity Tests

Stability

Tests

Antifungal

Tests


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PLANT IDENTIFICATION

The plant sample from Nueva Ecijawas identified and validated at theUST Herbarium by Prof. Rosie S.

Madulid.


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PLANT COLLECTION

The group collected a total of16kilograms of luyang dilaw prior to

plant extraction from Gapan, NuevaEcija where the plant is veryabundant.


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PLANT EXTRACTION

First thing to do prior to extraction, is air

drying.

The plant sample was introduced in an

Erlenmeyer flask together with distilled

waterand was subjected to heat prior toHYDRODISTILLATION.

After that, the extract was pulled out off the

collecting chamber and was placed in a

vessel with sodium sulfate

The rhizomes yielded 4.75%turmeric oil.


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IDENTIFICATION OF

TURMERONEOne of the well-known constituents of the luyang

dilaw, called TURMERONE was identified via

Thin Layer Chromatography (TLC) analysis.

Theoretically, turmerone has an rf value of

0.72, having a violet spot on the plate

being the mobile phase.


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PHYSICAL TESTS

TESTS AVERAGE

pH 4.5

Specific Gravity 1.095

Refractive Index 1.461

Organoleptic description: The turmeric oil has physical attributes of having a pale

yellow to yellow color, and is a slightly viscous liquid, and has a recognizablearoma.

Turmeric Oil Data

Solubility

The sample was found to be immiscible in water, propylene glycol, glycerol,

phosphate buffer pH 7.2, 0.1N NaOH, 0.1N HCl, and miscible in, methanol

and 95% ethanol.


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PHYSICAL TESTS

Ethanol + Oil Solubility Glycerin + Oil Solubility NaOH + Oil Solubility

PEG + Oil Solubility Propylene Glycol + Oil

SolubilityTurmeric Oil contained

in a Pycnometer


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ANTIFUNGAL TESTS

Minimum Inhibitory ConcentrationCONCENTRATION GROWTHTRIAL 1 TRIAL 2 TRIAL 3

4TH DAY 7TH

DAY

10TH

DAY

4TH DAY 7TH

DAY

10TH DAY 4TH DAY 7TH DAY 10TH

DAY

Blank + + + + + + + + +

2% Ketoconazole - - - - - - - - -

Mineral Oil + + + + + + - + -

Pure Turmeric Oil - - + - - + - - -

50% - - + - - + - - -

25% - - - - - - - - -

12.5% - - - - - - - - -

6.25% - - - - - - - - -3.125% - - - - - - - - -

1.563% - - - - - + - - -

0.781% - - - - - + - - -

0.391% - - - - - + - + +

0.195% - - - - - + - - -

0.098% - - - - - + - - -


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ANTIFUNGAL TESTS

Antifungal Susceptibility Test

The essential oil was evidently susceptible to thetest organism T. rubrum until the 5th dilution(3.125%)

It managed to inhibit fungal growth until the 4th

day of observation with corresponding decreasein fungicidal activity the following days.


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Antifungal Susceptibility testing


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MICROBIAL LIMIT TESTS

After 24 hours incubation period, theessential oil impeded the growth ofmicroorganisms against the growth

medium, TSA.


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STABILITY TESTS

Photoreaction

- No significant changes were observed

via organoleptic examination and TLC.

Hygroscopicity

- Upon exposure to varying humidity for 24 hours,the essential oil gained weight, thus, it is

observed to be deliquescent.


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COMPATITIBILITY TESTS

Thermal Analysis

Based on the results of the DTA, turmeric oil is

compatible with cetyl alcohol and white wax.

Sodium borate and mineral oil formed a different

compound with the turmeric oil at a certain

temperature, leading them to chemically

incompatible.

Formulation


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DTA

Sodium borate & Turmeric Oil


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DTA

Mineral Oil & Turmeric Oil


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FORMULAreference: USP 31

Essential Oil (Luyang Dilaw)4.0 ml

Cetyl Alcohol.62.5 g

White Wax.60.0 g

Sodium Borate2.5 g

Mineral Oil...280.0 ml

Purified Water to make...1000.0 g


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IPQC

The pH of the cream is 7.


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CONCLUSION

The essential oil was proven to have antifungal

activity against T. rubrum. Having known its

potential, the group tested it in a specific dosage

form, cream.

The cream manifested almost the same degree

of efficacy as that of the oil and the positivecontrol, 2% Ketoconazole.


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RECOMMENDATION

Various fungi on a broad spectrum should beexperimented upon as well.

Different kinds of excipients should also be taken into

consideration to produce a more compatible

formulation for the cream.

Use of test animals forin vivo tests.

A cream having the active ingredient of luyang dilaw be

brought in the market one day.

Thesis Presentation 30-09-11

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