Thermo Scientific HyperSep Protein Precipitation Plate Effective removal of proteins using the CRASH method • Hydrophobic/oleophobic frits to enable only precipitation of proteins • Pore size optimized for consistent flowrate from well to well • Specially selected polypropylene for low extractables Part of Thermo Fisher Scientific chromatography HyperSep ™ Protein Precipitation Plate Biological samples commonly contain proteins that interfere with downstream analyses. The Thermo Scientific HyperSep Protein Precipitation Plate uses the CRASH method, in which the protein is denatured with acetonitrile and the flocculant filtered out, allowing 96 samples to be processed at one time. The HyperSep Protein Precipitation Plate is based on an industry-standard 96-well plate footprint. Protein ‘crashes’ out of solution and precipitates directly into each well when acetonitrile is added. This solves all common problems associated with the CRASH technique of protein clean-up.The novel dual frit, hydrophobically treated matrix means that there is no ‘wetting out’ and leakage of the sample through the plate before the application of vacuum. The frits are Hydrophobic/Olephobic.This retains sample/acetonitrile in the well to allow precipitation of the proteins only when the vacuum is applied. The pore size of the frits is optimized to allow ideal flow rate through each well. Critical Factors in Performance A number of factors are critical to achieving good separations: Filter Leakage When acetonitrile is added to denature the sample plasma protein, it is important that this doesn’t immediately ‘wet out’ the filter and cause the filtration plate to leak. Flow Rate Consistent flow rate of the sample when the vacuum applied is a major advantage, as it leads to better reproducibility from sample to sample. Product Specifications