Thermo Scientific Pierce Protein Molecular Weight Marker Thermo Scientific … · 2013-04-04 · Thermo Scientific PageRuler Unstained Protein Ladders Thermo Scientific PageRuler

Introducing Thermo Scientific

Pierce Prestained and Unstained

Protein Ladders for easy protein

molecular weight determination.

Fermentas

now sold as

Thermo Scientific

Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe SuperSignal Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.

Electrophoresis Gel Stains GuideThis 21-page brochure summarizes the key features and benefits of Thermo Scientific Pierce Staining Products. Easy-to-follow protocols with schematics demonstrate just how easy staining can be. To download this technical handbook, visit www.thermoscientific.com/pierce

5µL 10µL 5µL 10µL

150kDa

100kDa

80kDa

60kDa

50kDa

40kDa

30kDa

20kDa

4-12% Bis-Tris 4-20% Tris-Glycine

Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific Pierce Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.

Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific PageRuler Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin conjugates.

Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific PageRuler and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.

Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific Spectra Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.

with new protein ladder choices.Reach greater highs (and lows)

PageRuler PrestainedProtein Ladder

PageRuler PlusPrestained Protein Ladder

Prestained ProteinMolecular Weight Marker

Unstained ProteinMolecular Weight Marker

Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase

Bovine serum albumin

Ovalbumin

Lactate dehydrogenase

REase Bsp98I

β-lactoglobulinLysozyme

8-16% Tris-glycine SDS-PAGE

Product #’s 26612, 26613


Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE



Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE

Product # 26632


Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE


Product #’s 26610, 26611

Gel

Gel

Spectra MulticolorHigh Range Protein Ladder

Spectra MulticolorBroad Range Protein Ladder

PageRulerUnstained Protein Ladder

PageRuler Unstained Broad Range Protein Ladder

PageRuler Unstained Low Range Protein Ladder

Spectra MulticolorLow Range Protein Ladder

18% SP Tris-tricine SDS-PAGESP - Small Peptide

SDS - polyacrylamide gel with higher crosslinking, C=5%

Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDaPageRuler PrestainedProtein Ladder




Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa

© 2011 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Novex and NuPAGE are registered trademarks of Invitrogen. Ready Gel is a registered trademark of Bio Rad. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

BN

0307

116

03/

11 P

rinte

d in

the

U.S

.

In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com

In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s PI-26612, PI-26613




Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE




18% SP Tris-tricine SDS-PAGE

Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa






Fermentas

now sold as

Thermo Scientific

Thermo Scientific Pierce Protein Research Product Portfolio now includes protein ladders made with Fermentas technology. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our unstained protein ladders.

Highlights:

• Cost effective – high quality without the high price; average cost of less than $1 per gel application

• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination

• Convenient – protein ladders are ready to load with no heating or boiling required

• Reliable – exceptional lot-to-lot consistency and reproducibility




150kDa

100kDa

80kDa

60kDa

50kDa

40kDa

30kDa

20kDa






with new protein ladder choices. Reach greater highs (and lows)





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa


BN

0307

116

03/

11 P

rinte

d in

the

U.S

.







Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa


Reach new heights in gel electrophoresis

0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300

Spectra Multicolor High Range

Spectra Multicolor Broad Range

PageRuler Unstained Broad Range

PageRuler Plus Prestained Ladder

PageRuler Unstained Ladder

PageRuler Prestained Ladder

Pierce Prestained Marker

Pierce Unstained Marker

PageRuler Unstained Low Range

Spectra Multicolor Low Range

Protein Ladder Range (kDa)

The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.

Unstained Ladder

Prestained Ladder

Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders

Rem

ove

this

pan

el a

t per

f and

reta

in fo

r fut

ure

refe

renc

e.

Reference Guide: Migration Patterns in Different Electrophoresis Conditions

Mig

ratio

n Pa

ttern

s of

Spe

ctra

Mul

ticol

or

Hig

h Ra

nge

and

Low

Ran

ge P

rote

in L

adde

rM

igra

tion

Patte

rns

of S

pect

ra M

ultic

olor

B

road

Ran

ge P

rote

in L

adde

rM

igra

tion

Patte

rns

of P

ageR

uler

Pr

esta

ined

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Pag

eRul

er P

lus

Pres

tain

ed P

rote

in L

adde

r

SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex® precast gels.††† – migration patterns were determined using respective Ready Gel® precast gel.

% le

ngth

of g

el 40

25

15

1052

40

25

15

1052

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

52

40

25

15

10

52

40

25

15

1052

40

25

15

105

40

15

1052

Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine

Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †

Running buffer Tris-Tricine SDS

Apparent Molecular Weights, kDa

10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

300250

180

130

100

70

5040

300

250

180

130

100

300250180

130

100

70

50

40

300

250

180

130

100

70

5040

270205

150

120

85

65

50

40

270205150120

85

65

50

40

270185140115

80

65

50

40

300250180

130

100

70

50

40

300250180130100

70

50

40

Thermo Scientific Spectra Multicolor High Range Protein LadderGel type Tris-Glycine Tris-Acetate†† Bis-Tris††

Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS

Apparent Molecular Sizes (kDa)

10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

260

140100

70

504035

25

15

10

260

14010070

50

40

35

25

15

10

260

140

100

70

50

40

35

25

15

260

140100

70

50

40

35

25

15

26014010070

50

4035

25

15

10

26014010070

50

40

35

25

15

10

225

120

85

65

40

50

3025

225

120

85

65

40

50

30

25

225

115

80

65

35

50

30

25

1510

225

11580

65

35

50

30

25

15

225

1158065

35

50

30

25

15

235

120

8070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

235

1208070

40

50

3025

15

10

260

140

100

70

50

4035

25

15

10

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder

Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††

Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%

Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES


10

20

30

40

50

60

70

80

90

100

205

120

85

65

30

50

25

250

100

70

35

55

25

15

130

19011580

70

30

50

25

15

10

190

1158070

30

50

25

15

10

185

115

80

65

30

50

25

1510

250

100

70

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

100

70

35

55

25

15

130

250

10070

35

55

25

130

10

250

10070

35

55

25

15

130

10

205

120

85

65

30

50

25

185

1158065

30

50

25

15

185

1158065

30

50

25

15

1901158070

30

50

25

15

10

% le

ngth

of g

el

15

Thermo Scientific PageRuler Plus Prestained Protein Ladder





10

20

30

40

50

60

70

80

90

100

17013010070

55

40

35

25

15

10

170

130

100

70

55

40

35

25

15

10

17013010070

55

4035

25

15

10

170130

100

70

55

40

35

25

15

170130100

70

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

150

120

85

65

50

403025

150

120

85

65

50

40

30

25

115140

80

65

50

40

30

25

15

10

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

10

1401158070

50

40

30

25

15

10

1401158070

5040

30

25

15

10

Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.

†† – Migration patterns were determined using respective NuPAGE® precast gels.

% le

ngth

of g

el

Thermo Scientific PageRuler Prestained Protein Ladder





10

20

30

40

50

60

70

80

90

100

Ordering Information

Product # DescriptionPkg. Size

PI-26610 Pierce® Unstained Protein MW Marker

2 x 1mL

PI-26611 Pierce Unstained Protein MW Marker

25µL

PI-26612 Pierce Prestained Protein MW Marker

500µL


25µL

PI-26614 PageRuler Unstained Protein Ladder

2 x 250µL


25µL

PI-26616 PageRuler Prestained Protein Ladder

2 x 250µL


10 x 250µL


25µL

PI-26619 PageRuler Plus Prestained Protein Ladder

2 x 250µL


10 x 250µL


25µL

PI-26634 Spectra™ Multicolor Broad Range Protein Ladder

2 x 250µL

PI-26623 Spectra Multicolor Broad Range Protein Ladder

10 x 250µL


50µL

PI-26625 Spectra Multicolor High Range Protein Ladder

2 x 250µL


50µL

PI-26628 Spectra Multicolor Low Range Protein Ladder

250µL


25µL

PI-26630 PageRuler Unstained Broad Range Protein Ladder

2 x 250µL


25µL

PI-26632 PageRuler Unstained Low Range Protein Ladder

2 x 250µL


25µL

PI-84785 SuperSignal Molecular Weight Protein Ladder

250µL

PI-84786 SuperSignal Enhanced Molecular Weight Protein Ladder

250µL

Application

Thermo Scientific Pierce Protein Ladders SDS PAGE Western blotting

Product Name Product #

Former Fermentas Part # # Proteins

MW Range

Reference Bands


PI-26630 SM1881 11 5-250kDa 20, 50 and 100kDa

Best — — Best — — Good†


PI-26632 SM1891 8 3.4-100kDa 25kDa Best — — Best — — Good†

PageRuler Unstained Protein Ladder

PI-26614 SM0661 14 10-200kDa 50kDa Good — — Good — — Good†

Pierce Unstained Protein Molecular Weight Marker

PI-26610 SM0431 7 14.4-116kDa

— Good — — Good — — —

SuperSignal Molecular Weight Protein Ladder

PI-84785, PI-84786

— 8 20-150kDa — Good — — Good — Good Best

PageRuler Prestained Protein Ladder

PI-26616, PI-26617

SM0671, SM0672

10 10-170kDa Green 10kDa; Orange 70kDa

Good Good Good — Good‡ Good —

PageRuler Plus Prestained Protein Ladder

PI-26619, PI-26620

SM1811, SM1812

9 10-250kDa Green 10kDa; Orange 25 and 70kDa


Spectra Multicolor Broad Range Protein Ladder

PI-26634, PI-26623

SM1841, SM1842

10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa

Good Best Best — Good‡ Best —

Spectra Multicolor Low Range Protein Ladder

PI-26628 SM1861 6 1.7-40kDa Green 10kDa; Orange 40kDa


Spectra Multicolor High Range Protein Ladder

PI-26625 SM1851 8 40-300kDa Green 50kDa; Orange 70 and 300kDa


Pierce Prestained Protein Molecular Weight Marker

PI-26612 SM0441 6 20-120kDa — Good Good Good — Good Good —

† with Strep-Tactin® HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region

Uns

tain

ed p

rote

in la

dder

sPr

esta

ined

pro

tein

ladd

ers

Prot

ein

MW

Dete

rmin

atio

nPr

otei

n

band

visu

aliza

tion

Mon

itorin

g

elec

troph

ores

is

run Co

omas

sie/

Silv

er/

Fluo

resc

ent

Stai

ning

NIR

de

tect

ion

Mon

itorin

g pr

otei

n

trans

fer

Chem

ilum

ines

cent

band

visu

aliza

tion


0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300













Unstained Ladder

Prestained Ladder


Rem

ove

this

pan

el a

t per

f and

reta

in fo

r fut

ure

refe

renc

e.

Reference Guide: Migration Patterns in Different Electrophoresis Conditions

Mig

ratio

n Pa

ttern

s of

Spe

ctra

Mul

ticol

or

Hig

h Ra

nge

and

Low

Ran

ge P

rote

in L

adde

rM

igra

tion

Patte

rns

of S

pect

ra M

ultic

olor

B

road

Ran

ge P

rote

in L

adde

rM

igra

tion

Patte

rns

of P

ageR

uler

Pr

esta

ined

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Pag

eRul

er P

lus

Pres

tain

ed P

rote

in L

adde

r


% le

ngth

of g

el 40

25

15

1052

40

25

15

1052

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

52

40

25

15

10

52

40

25

15

1052

40

25

15

105

40

15

1052





10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el300250

180

130

100

70

5040

300

250

180

130

100

300250180

130

100

70

50

40

300

250

180

130

100

70

5040

270205

150

120

85

65

50

40

270205150120

85

65

50

40

270185140115

80

65

50

40

300250180

130

100

70

50

40

300250180130100

70

50

40




10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

260

140100

70

504035

25

15

10

260

14010070

50

40

35

25

15

10

260

140

100

70

50

40

35

25

15

260

140100

70

50

40

35

25

15

26014010070

50

4035

25

15

10

26014010070

50

40

35

25

15

10

225

120

85

65

40

50

3025

225

120

85

65

40

50

30

25

225

115

80

65

35

50

30

25

1510

225

11580

65

35

50

30

25

15

225

1158065

35

50

30

25

15

235

120

8070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

235

1208070

40

50

3025

15

10

260

140

100

70

50

4035

25

15

10






10

20

30

40

50

60

70

80

90

100

205

120

85

65

30

50

25

250

100

70

35

55

25

15

130

19011580

70

30

50

25

15

10

190

1158070

30

50

25

15

10

185

115

80

65

30

50

25

1510

250

100

70

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

100

70

35

55

25

15

130

250

10070

35

55

25

130

10

250

10070

35

55

25

15

130

10

205

120

85

65

30

50

25

185

1158065

30

50

25

15

185

1158065

30

50

25

15

1901158070

30

50

25

15

10

% le

ngth

of g

el

15






10

20

30

40

50

60

70

80

90

100

17013010070

55

40

35

25

15

10

170

130

100

70

55

40

35

25

15

10

17013010070

55

4035

25

15

10

170130

100

70

55

40

35

25

15

170130100

70

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

150

120

85

65

50

403025

150

120

85

65

50

40

30

25

115140

80

65

50

40

30

25

15

10

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

10

1401158070

50

40

30

25

15

10

1401158070

5040

30

25

15

10



% le

ngth

of g

el






10

20

30

40

50

60

70

80

90

100




2 x 1mL


25µL


500µL


25µL


2 x 250µL


25µL


2 x 250µL


10 x 250µL


25µL


2 x 250µL


10 x 250µL


25µL


2 x 250µL


10 x 250µL


50µL


2 x 250µL


50µL


250µL


25µL


2 x 250µL


25µL


2 x 250µL


25µL


250µL


250µL

Application




MW Range

Reference Bands


PI-26630 SM1881 11 5-250kDa 20, 50 and 100kDa







PI-26610 SM0431 7 14.4-116kDa

— Good — — Good — — —


PI-84785, PI-84786



PI-26616, PI-26617

SM0671, SM0672




PI-26619, PI-26620

SM1811, SM1812




PI-26634, PI-26623

SM1841, SM1842












Uns

tain

ed p

rote

in la

dder

sPr

esta

ined

pro

tein

ladd

ers

Prot

ein

MW

Dete

rmin

atio

nPr

otei

n

band

visu

aliza

tion

Mon

itorin

g

elec

troph

ores

is

run Co

omas

sie/

Silv

er/

Fluo

resc

ent

Stai

ning

NIR

de

tect

ion

Mon

itorin

g pr

otei

n

trans

fer

Chem

ilum

ines

cent

band

visu

aliza

tion


0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300













Unstained Ladder

Prestained Ladder


Rem

ove

this

pan

el a

t per

f and

reta

in fo

r fut

ure

refe

renc

e.

Reference Guide: Migration Patterns in Different Electrophoresis ConditionsM

igra

tion

Patte

rns

of S

pect

ra M

ultic

olor

H

igh

Rang

e an

d Lo

w R

ange

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Spe

ctra

Mul

ticol

or

Bro

ad R

ange

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Pag

eRul

er

Pres

tain

ed P

rote

in L

adde

rM

igra

tion

Patte

rns

of P

ageR

uler

Plu

s Pr

esta

ined

Pro

tein

Lad

der


% le

ngth

of g

el 40

25

15

1052

40

25

15

1052

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

52

40

25

15

10

52

40

25

15

1052

40

25

15

105

40

15

1052





10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

300250

180

130

100

70

5040

300

250

180

130

100

300250180

130

100

70

50

40

300

250

180

130

100

70

5040

270205

150

120

85

65

50

40

270205150120

85

65

50

40

270185140115

80

65

50

40

300250180

130

100

70

50

40

300250180130100

70

50

40




10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

260

140100

70

504035

25

15

10

260

14010070

50

40

35

25

15

10

260

140

100

70

50

40

35

25

15

260

140100

70

50

40

35

25

15

26014010070

50

4035

25

15

10

26014010070

50

40

35

25

15

10

225

120

85

65

40

50

3025

225

120

85

65

40

50

30

25

225

115

80

65

35

50

30

25

1510

225

11580

65

35

50

30

25

15

225

1158065

35

50

30

25

15

235

120

8070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

235

1208070

40

50

3025

15

10

260

140

100

70

50

4035

25

15

10






10

20

30

40

50

60

70

80

90

100

205

120

85

65

30

50

25

250

100

70

35

55

25

15

130

19011580

70

30

50

25

15

10

190

1158070

30

50

25

15

10

185

115

80

65

30

50

25

1510

250

100

70

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

100

70

35

55

25

15

130

250

10070

35

55

25

130

10

250

10070

35

55

25

15

130

10

205

120

85

65

30

50

25

185

1158065

30

50

25

15

185

1158065

30

50

25

15

1901158070

30

50

25

15

10

% le

ngth

of g

el

15






10

20

30

40

50

60

70

80

90

100

17013010070

55

40

35

25

15

10

170

130

100

70

55

40

35

25

15

10

17013010070

55

4035

25

15

10

170130

100

70

55

40

35

25

15

170130100

70

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

150

120

85

65

50

403025

150

120

85

65

50

40

30

25

115140

80

65

50

40

30

25

15

10

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

10

1401158070

50

40

30

25

15

10

1401158070

5040

30

25

15

10



% le

ngth

of g

el






10

20

30

40

50

60

70

80

90

100




2 x 1mL


25µL


500µL


25µL


2 x 250µL


25µL


2 x 250µL


10 x 250µL


25µL


2 x 250µL


10 x 250µL


25µL


2 x 250µL


10 x 250µL


50µL


2 x 250µL


50µL


250µL


25µL


2 x 250µL


25µL


2 x 250µL


25µL


250µL


250µL

Application




MW Range

Reference Bands


PI-26630 SM1881 11 5-250kDa 20, 50 and 100kDa







PI-26610 SM0431 7 14.4-116kDa

— Good — — Good — — —


PI-84785, PI-84786



PI-26616, PI-26617

SM0671, SM0672




PI-26619, PI-26620

SM1811, SM1812




PI-26634, PI-26623

SM1841, SM1842












Uns

tain

ed p

rote

in la

dder

sPr

esta

ined

pro

tein

ladd

ers

Prot

ein

MW

Dete

rmin

atio

nPr

otei

n

band

visu

aliza

tion

Mon

itorin

g

elec

troph

ores

is

run Co

omas

sie/

Silv

er/

Fluo

resc

ent

Stai

ning

NIR

de

tect

ion

Mon

itorin

g pr

otei

n

trans

fer

Chem

ilum

ines

cent

band

visu

aliza

tion





Fermentas

now sold as

Thermo Scientific

Thermo Scientific Pierce Protein Research Product Portfolio now includes protein ladders made with Fermentas technology. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our unstained protein ladders.

Highlights:

• Cost effective – high quality without the high price; average cost of less than $1 per gel application

• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination

• Convenient – protein ladders are ready to load with no heating or boiling required

• Reliable – exceptional lot-to-lot consistency and reproducibility




150kDa

100kDa

80kDa

60kDa

50kDa

40kDa

30kDa

20kDa






with new protein ladder choices. Reach greater highs (and lows)





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa


BN

0307

116

03/

11 P

rinte

d in

the

U.S

.







Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I












Product # PI-26632


Product #’s PI-26616, PI-26617, PI-26618


Product #’s PI-26619, PI-26620, PI-26621



Gel

Gel










~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa


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