Thermo Scientific Dionex Corona Veo Charged Aerosol ... · Charged aerosol technology. 4 The Thermo Scientific ™ Dionex™ Corona™ Veo™ detector improves the capabilities and

See clearly hidden peaks revealed

Thermo Scientific Dionex Corona Veo Charged Aerosol Detector

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The analyte detection challenge

No single liquid chromatography (LC) detector delivers ideal results.

Often, one analyte responds more strongly than another, or may

not respond at all. What is most desired is the ability to accurately

measure a wide range of analytes with consistent response.

Charged aerosol detection (CAD) is a revolutionary technology that

will change the way you view every sample. This technique provides

consistent analyte response independent of chemical structure and

gives greater sensitivity over a wider dynamic range.

Hidden peaks revealed

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Void + Cl-Propanolol

Ketoprofen Verapamil

UV at 220 nm

UV at 254 nm

Charged Aerosol Detection

MS TIC(+ ion scan 150-500AMU)

The area percent response is accurate and consistent across

a wide spectrum of molecules from ions to proteins.

With the flexibility and performance for analytical R&D, and

the simplicity and reproducibility needed for manufacturing

QC/QA, charged aerosol detection can be used for the analysis

of pharmaceuticals (large and small molecule), biomolecules,

foods and beverages, specialty chemicals, and polymers.

A charged aerosol detector opens new opportunities for discovery and routine analysis – the ideal detector for every lab.

Comparison of Charged Aerosol Detection to UV and MS

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Charged aerosol detection measures signals that are in direct

proportion to the amount of analytes present in a sample. These

signals are derived from the measurement of an electrical charge

originating from ionized nitrogen that is diffusionally transferred

to the surface of the analyte particle. This technology quantifies

the analyte particles that attract this charge, including those

that cannot ionize or do not have chromophores. The result is

accurate and consistent response, regardless of analyte structure.

With charged aerosol detection, you can measure any nonvolatile

and most semivolatile analytes.

Understanding charged aerosol detection

Three Simple Steps

Charged aerosol detection begins by nebulizing the eluent into droplets, which are subsequently dried into particles. The particle size increases with the amount of analyte.

A stream of charged gas collides with the analyte particles. The charge is then transferred to the particles – the larger the particles, the greater the charge.

The particles are transferred to a collector where the charge is measured by a highly sensitive electrometer. This generates a signal in direct proportion to the quantity of analyte present.

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Charged aerosol technology

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The Thermo Scientific™ Dionex™ Corona™ Veo™ detector improves

the capabilities and extends the advantages of charged aerosol

detection. The Corona Veo detector combines all the benefits of

CAD for the high speed and increased resolution of UHPLC, along

with improved low flow performance for micro LC. This detector

widens the envelope of LC conditions for mobile phase composition

to take advantage of ever advancing column technologies.

• Simple, intuitive operation

• Enhanced linear dynamic range

• Sub-nanogram sensitivity

• Expanded flow rate range optimized from 0.01 to 2.0 mL/min

The Corona Veo detector incorporates an advanced concentric

nebulizer design that facilitates the use of an expanded range of

flows and eluents:

• Easy-to-access design for maximized uptime

• Micro LC flow optimized

• Designed for simple capillary connections

An evolution for charged aerosol detection

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The UHPLC analysis of amikacin impurities using micro LC flow rate of 50uL/min

The FocusJet™ nebulizer

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The Corona Veo detector is designed to integrate into any liquid

chromatographic system, HPLC or UHPLC, from any manufacturer.

The stackable design and rugged construction allow positioning

anywhere within a system. Software drivers are available for the

Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data

System (CDS), as well as ChemStation®, EZChrom®, OpenLab®, and

Empower® 2 and 3 software.

The UltiMate SolutionCombine the Corona Veo detector with the Thermo Scientific™

Dionex™ UltiMate™ 3000 LC system to provide an ideal solution for

approximation of relative analyte concentrations, even when standards

are not available. Widen the scope of your detection possibilities using

orthogonal and complementary solutions by pairing with UV or mass

spectrometry (MS). This approach maximizes the amount of compound

data obtained from a single analytical run.

Easy integration with any LC System

New bioinert 1/32” capillary Thermo Scientific™ Dionex™ Viper™ Fingertight Fitting System provides virtually zero-dead-volume connections for any column, at any flow rate up to 1250 bar. The Viper design ensures easy connections of all fluidics for any LC system connected to the Corona Veo charged aerosol detector.

PEEK® Seal

Removable Knurl

Adapter (no ferrule!)

Fingertight Screw

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Flexibility for many analytesCharged aerosol detection is an ideal primary LC detection

technology that delivers predictable results without intricate

optimization. There are a minimum of parameters to optimize

enabling you to quickly begin generating data. This detector

can be used in a range of applications from basic research to

quality control.

Lipids

Lipids, including fats and oils, contain molecules with a wide

variety of structural properties and are found throughout many

products and industries. These are traditionally difficult to

characterize with conventional detection technologies. Charged

aerosol detection is a powerful tool for lipid analysis due to its

structure-independent response.

Pharmaceutical Product Characterization

Using HPLC-HILIC methodologies, counterions along with their

parent compound can be analyzed simultaneously with the

Corona Veo detector. This approach helps speed selection of

final product formulations.

Industrial Water Treatment

Polyacrylic acid, a biodegradable water soluble polymer and

environmentally toxic agent, is used as an anti-scaling inhibitor in

circulating water systems. The low levels present are difficult to

characterize using standard methods. This example shows detection by

CAD at low ppb levels.

Flexibility for many analytes

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Fast analysis of diclofenac, its counterions, and impurities

Polyacrylic Acid in cooling water

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Triglycerides

Paraffins

Alkyl Esters

Fatty Acids Fatty Alcohols

Diglycerides Monoglycerides

Universal lipids analysis of algal oils

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Carbohydrates

Sugar analysis using HPLC-RI or HPAE-PAD is limited to isocratic

methods or requires complicated methodologies using dedicated

instrumentation. The Corona Veo detector offers the flexibility to use

reversed-phase gradients and HILIC modes of separation on any LC

system with dilute-and-shoot simplicity.

Power Function - Enhanced Linearization

The Power Function provides several benefits when using

charged aerosol detection. The use of this built-in feature

can provide quantitative results from unresolved peaks in a

chromatographic analysis, and simplify calibration curves for

improved mass balance determinations.

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Calibration Data No PFV – Polynomial Regression Fit

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Calibration Data using PFV – Linear Regression Fit

UHPLC characterization of sugars in apple juice

Extended linear dynamic range using the Power Function Value (PFV)

The Chromeleon Chromatography Data System delivers rich, intelligent

functionality with Operational Simplicity™.

• Reduced training effort for your lab staff-one single software

package instead of several

• Comprehensive suite of Chromeleon CDS productivity tools from

dynamic data linking, instant processing, to SmartPeaks™ detection

• One set of methods, one set of reports, and one central location for

your chromatographic data

• Seamless integration and access to the online applications and

methods library

Chromeleon 7.2 CDS combines separation (LC/IC/GC) and

MS in an enterprise (client-server) environment to streamline

your chromatography and quantitation workflows in one application.

Everything you need is within easy reach to boost your overall

lab productivity.

Chromeleon 7.2 CDS simply intelligent

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