The ZIRC Nursery The ZIRC Nursery The Basics of a Successful The Basics of a Successful Zebrafish Nursery System Zebrafish Nursery System European Zebrafish Meeting 2009 European Zebrafish Meeting 2009 Rome, Italy Rome, Italy Carrie L. Barton Carrie L. Barton Zebrafish International Zebrafish International Resource Center Resource Center University of Oregon University of Oregon
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The ZIRC Nursery The Basics of a Successful Zebrafish Nursery System European Zebrafish Meeting 2009 Rome, Italy Carrie L. Barton Zebrafish International.
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The ZIRC Nursery The ZIRC Nursery The Basics of a SuccessfulThe Basics of a SuccessfulZebrafish Nursery SystemZebrafish Nursery System
European Zebrafish Meeting 2009European Zebrafish Meeting 2009
Rome, ItalyRome, Italy
Carrie L. BartonCarrie L. Barton
Zebrafish International Resource Zebrafish International Resource CenterCenter
University of OregonUniversity of Oregon
ZIRC Autonursery System ZIRC Autonursery System
• Modular rack systemModular rack system
• Fully automatic water deliveryFully automatic water delivery
• 500 ml polycarbonate tanks 500 ml polycarbonate tanks independent, adjustable water independent, adjustable water inlet inlet
CapacityCapacity
Functional Capacity = 12,000 fishFunctional Capacity = 12,000 fish
Total Capacity = 24,000 fishTotal Capacity = 24,000 fish
Density = 50 fish per tankDensity = 50 fish per tank
ZIRC Nursery Feeding and Water ScheduleZIRC Nursery Feeding and Water Schedule
• Water Flow Progression: Static-12hr-24hrWater Flow Progression: Static-12hr-24hr
• Food Progression and Size Overlap: Paramecia-Powder-ArtemiaFood Progression and Size Overlap: Paramecia-Powder-Artemia
The FundamentalsThe Fundamentals
• Water QualityWater Quality
• Food QuantityFood Quantity
• Food SizeFood Size
Goal: Embryo to Adult Effectively Goal: Embryo to Adult Effectively and Efficientlyand Efficiently
Water QualityWater Quality• Pre-nurseryPre-nursery
• Static vs. Running WaterStatic vs. Running Water
• This will largely depend on your This will largely depend on your
particular systemparticular system
• Flow RateFlow Rate
• Clean WaterClean Water
vs.vs.
• Food Retention/Wash OutFood Retention/Wash Out
Food QuantityFood Quantity
• Relationship to Water QualityRelationship to Water Quality• Running Water = More FoodRunning Water = More Food• Static System = Requires More Precise Food DeliveryStatic System = Requires More Precise Food Delivery
• Number of Feedings Per DayNumber of Feedings Per Day• 2-3 is Ideal2-3 is Ideal
• Amount Administered at Each FeedingAmount Administered at Each Feeding• Density of Fish/Size of TankDensity of Fish/Size of Tank• Avoid OverfeedingAvoid Overfeeding
• Overfeeding = Excessive Food Build Up = Poor Water QualityOverfeeding = Excessive Food Build Up = Poor Water Quality• Powdered Feed and Artemia Can Adversely Effect Your Powdered Feed and Artemia Can Adversely Effect Your
Water Quality if Over FedWater Quality if Over Fed• Paramecia and Rotifers Can be Fed More LiberallyParamecia and Rotifers Can be Fed More Liberally
Food ChoiceFood Choice
Commonly Use Foods:Commonly Use Foods:• ParameciaParamecia• RotifersRotifers