The role of apoptotic and necroptotic PBMC secretome in ... › hp › fileadmin › thorax... · January 2019 1 The role of apoptotic and necroptotic PBMC secretome in angiogenesis

January 2019

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The role of apoptotic andnecroptotic PBMC secretome in angiogenesis and wound healing19.01.2019

OrganisationseinheitTitel der Präsentation ODER des Vortragenden

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γ-irradiation activates the Necroptosis pathway

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Experimental design

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Positive selection of PBMC subsets

Peripheral blood mononuclear cells

• CD 14 Monocytes

• CD 4 T-Helper cells

• CD 8 Cytotoxic T-cells

• CD 56 Natural killer cells

• CD 19 B-cells

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Irradiation and Necroptosis

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p-MLKL

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Angiogenic potential of apoptotic and necroptotic PBMC?

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PBMCNK-cellsMonocytes

B cellsCD8 T cellsCD4 T cells

PBMC

NK-cells

Monocytes

CD4 T cells

CD8 T cells

B cells

Angiogenesis

Cytokine production TGFβ pathway

Response to hypoxiap53 pathway

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CD4 T cells

Monocytes

B cellsCD8 T cells

PBMC NK-cells

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non-

irrad

iate

d

vehi

cle

z-VA

D

Nec

rost

atin

p-MLKL

c-cas 3

zVA

D+

Nec

rost

atin

γ-irradiation

vehicle zVAD

NecrostatinzVAD +

Necrostatin

Thank you for your attention!

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Time and dose-dependent effects

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p-MLKL

Scanning electron microscopy

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-irradiated PBMC

Cell death

Biochemistry, Genetics and Molecular Biology » "Apoptosis and Medicine", book edited by Tobias M. Ntuli, ISBN 978-953-51-0701-9, Published: August 16, 2012 under CC BY 3.0 license. © The Author(s). Cell Death and Cancer, NovelTherapeutic Strategies By Silvina Grasso, M. Piedad Menéndez-Gutiérrez, et al DOI: 10.5772/51285

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