The project is the winner of the Foundation for Assistance to Small Innovative Enterprises (FASIE) set up by Russian Federation government Development.

The project is the winner of tThe project is the winner of the Foundation for Assistance to Small Innovative he Foundation for Assistance to Small Innovative Enterprises (FASIE) set up by Russian Enterprises (FASIE) set up by Russian Federation Federation governmentgovernment

Development of new methodDevelopment of new methodof vaccinations and treatmentof vaccinations and treatment

of infectious diseasesof infectious diseases with use of the with use of the device on the basis of structure with device on the basis of structure with

controlled energy material (CEM)controlled energy material (CEM)

First, there is chemical interaction under condition of contact between objects; second, there is influence of one object on another with the help of electromagnetic radiation, and in this case influence can occur on distance without direct contact of the objects. The second type of interaction in conditions of low-intensity radiation is possible exclusively in the case of the coincidence of object’s own frequencies.

Modern idea about mechanisms of interaction between material objects is related to the dual state of the substance.

Different mechanism for creation of specific immunity is offered - the material with properties of changed energy structure (СЕМ), electromagnetic spectrum of which is identical to electromagnetic spectrum of a virus or bacterium, performs the role of antibody. When the material with the changed energy structure (СЕМ), which has electromagnetic radiation spectrum, similar to the alien infectious agent, is administered into the organism, effect of vaccination or effect of therapy (through the period of temporary disease exacerbation) is achieved.

Different mechanism for creation of specific immunity

CEM structura

Human - Virus

The mechanisms of vaccination, consisting in creation of maximum specific cellular and humoral immunity against viruses and bacteria, are well investigated since times of L.Paster. In simplified form it could be presented as struggle of specific antibodies and cells of organism immune against alien antigenes. This method has a number of serious lacks, the main of which relates to poorly predicted immunity changes with possible allergization, long period of immunity formation, impossibility for steady immunity formation at fast mutation of viruses and bacteria.

The mechanisms of vaccination

Shema

Development of principally new method of vaccination of living organisms from infectious diseases, and also development of principally new technology of therapy for infectious diseases with the maximal antimicrobic and antiviral effects and the minimal influence on the environmental cells are suggested in the given project.

New Method of Vaccination

This method makes it possible very quickly to achieve specific immunity from fast mutating viruses and bacteria, it does not create effect of nonspecific allergization; the material, administered into the organism, is not toxic. Thus, there is a real mechanism for high-grade new stage of struggle against infectious diseases. The most acute opportunities of the new method are effective fight against fast mutating viruses and bacteria (SARS, AIDS, etc.) and struggle with especially dangerous infections, including way of struggle against bioterrorism.

This method makes it possible very quickly to achieve specific immunity from fast mutating viruses and bacteria

In the given project it is suggested to use special semi-conductor structures with controlled energy material (CEM), as vaccine or treatment.The resonant frequencies appropriate for DNA, cell membranes, etc. and its components are found in mm – IR range (Fig.1.1) .

Fig. 1.1 STRUCTURE of the CELL

Structure of the cell

Fig 1.2 THE ENERGY CHARACTERISTICS OF THE ORGAN

AT THE CELLULAR LEVEL

Violation of vital functions of cells of the organism leads to change of its energy structure in the given range. Each cell of the organism occupies only appropriated level in its own energy structure. Accordingly, in the energy spectrum of the organism each organ occupies only its characteristic area, consisting from the levels, appropriate for separate cells. Energy areas of separate human organs are divided by sites of the spectrum, where radiation is absent (Fig. 1.2).

Energy Characteristics of the

Organ

Biological interaction of cells of "host“-bioobject and microorganisms of the environment (2 variants of interaction - illness or symbiosis) is possible only in case, when own frequencies of energy spectrum of microorganism and cells of ”host”-organism coincide (positive coexistence – symbiosis or negative coexistence - illness).In case of spectra discrepancy, interaction at energy level is absent, which results in impossibility of biological coexistence of ”host“-bioobject and ”guest”-microorganism (Fig. 1.3).

Fig. 1.3 THE ENERGY CHARACTERISTICS OF THE HUMAN AND SOME INFORMATION DISEASES

Energy Characteristics of the Human

Sick human himself is a source of drug, because he has full spectrum of frequencies of pathogenic ”guest”-microorganisms.It is very important, that pathogenic ”guest”-microorganisms have sensitively to external influence, which is by several orders of magnitude greater (Fig. 1.4), than the sensitivity of cells of ”host”-organism (Fig. 1.5). This fact allows to influence selectively upon pathogenic ”guest” -microorganisms.

Fig. 1.4 Sensivity of guest microorganism

Fig. 1.5 Sensivity of organism –”host”Sensitivity of “Host” ans “Guest” Microorganisms

Therefore, with the purpose of prophylaxis of infectious disease it is suggested that preparation of "energy analogue” of pathogenic ”guest”-microorganism with use of the device on the basis of structure with controlled energy material (by method of recording of radiation of ”guest”-microorganism on the material carrier), with its subsequent incorporation into structure of the protected “host” organism (Fig. 1.6).

Fig 1.6 RECORDING OF THE SPECTRUM OF RADIATION FROM ”GUEST”-MICROORGANISM TO THE MATERIAL CARRIER

Recording of External Radiation Spectrum on

CEM

As numerous carried out experiments showed, similar procedure leads to blockade of energy spectrum, characteristic for pathogenic “guest”-microorganism, and, as a result, to “replacement” of “guest”-microorganism or another “spectrum guest” structure from “already occupied” energy level, i.e. to effect of vaccination of ”host”-organism (Fig. 1.7).

CEM in Practice

Fig. 1.7 STAGES OF VACCINATION

Stages CEM Vaccination

With the purpose of treatment of infectious disease it is suggested preparing of similar material and its inclusion into the long contact with the pacient’s ”host”-organism. (Fig. 1.8).

Stages of CEM Treatment

Fig 1.8 STAGES OF TREATMENT

Stages of CEM Treatment

In this case development of disease passes classical phases of natural recovery: short-term acute period with synchronization of pathogenic spectral components (it is necessary to apply profile pharmacological preparations, which will operate more effectively in this case), then suppression of vital activity of the “guest” -microorganism, related with deppression of their energy activity at all spectral levels, characteristic to them, and with replacement by radiation of the prepared device (Fig. 1.9).

Fig. 1.9 CLINICAL COURSE

Dynamic of treatment

Duration of treatment depends on the degree of disease development and features of duplication cycle of pathogenic “guest”-microorganism.The control device is created on the basis of molecular fluctuation light-scattering method for quality control of received recordings on devices, optimization of recording technology and stability of work of recording device. Action of this device is based on therecording-reproduction of spectrum of radiation of “guest”-microorganism (Fig. 1.6).

Control of CEM Recording

Fig 1.6 RECORDING OF SPECTRUM OF THE RADIATION FROM ”GUEST”-MICROORGANISM TO THE MATERIAL CARRIER

Control of CEM Recording

The first year of the project realization provides performance of the following stages of work:

1. Optimization of structure of devices with controlled energy material. The following things are provided: the choice of optimum carrier material in the view of recording quality of electromagnetic (EM) radiation of pathogenic “guest”-microorganism, technology of the preparation and expenses for manufacturing and application of the device.

2. Further development of quality monitoring of availability and quality of radiation recording on the material carrier is planned.

3. Further improvement of technology of recording on the material carrier is planned.

4. Selection of components for formation of “recipe" of recording is planned.

5. Perfomance of wide researches in vitro and on animals is planned.

Stages of CEM Project

Received results will serve as a basis for development and application of similar devices for prophilaxys and treatment of infectious diseases and should result in formation of new pathobiological directions, including medical and veterinary areas. In the future creation of anticancer vaccines with use of the given technology is also theoretically possible. The devices, developed in the project, should be characterized by simplicity of design, so that there was an opportunity for the organization of mass and inexpensive manufacture at profile microelectronics enterprises.

Stages of CEM Project

Expected project results of the first year :1. The device and technologies of EM radiation recording of

pathogenic cells of ”guest”-microorganisms on different material carriers.

2. Upgrade of the device for evaluation of quality of recording. The further development of an optical and electronic device circuit is provided; development of procedure of measurement and rating results of measurement algorithms (with development of the software); development of quality rating recording scales.

3. "Compounding" of preparations for formation of the electromagnetic recipe for vaccination and treatment.

4. Methods of application of the device with recording biologically active "recipe".

5. Tests on animals, clinical tests.Stages of CEM Project

II. The description of the recording device

The material carrier has 2 steady states, and the first corresponds to its cold state, and the second is achieved, when the carrier is given voltage, sufficient for surmounting the energy barrier. The time sequence of transition of the carrier into excited status (t1) and return to the initial state after contact with the ”guest” microorganism is given below with the purpose of recording its radiation with subsequent reproduction.

Description of the Recording Device

Fig. 2.1 DIAGRAM OF THE VOLTAGE TO THE CARRIER

Description of the

Recording Device

Fig. 2. 2 WITHOUT THE VOLTAGE (INITIAL STATE)

Description of the Recording Device

Fig. 2.3 WITH THE VOLTAGE

Description of the

Recording Device

Fig.2.4 CONTACT TO THE OBJECT (”GUEST” MICROOORGANISM)

Description of the

Recording Device

Fig. 2.5 WE REMOVE THE VOLTAGE

Description of the

Recording Device

Fig. 2.6 WE REMOVE THE OBJECT (”GUEST”MICROORGANISM )

FROM CARRIER

Description of the

Recording Device

Fig. 2.7 GRADUAL RELAXATION (RETURN TO INITIAL STRUCTURE WITHOUT THE VOLTAGE) WITH RE-EMISSION THE SIGNAL APPROPRIATED TO THE ”GUEST” MICROORGANISM.TIME OF RADIATION IS LIMITED.

Description of the

Recording Device

APPENDIXESAPPENDIXES1.1. QUALITY MONITORING OF RECORDING ON THE QUALITY MONITORING OF RECORDING ON THE

CARRIERCARRIERAPPENDIX 1

2.2. STRUCTURAL MEMORY OF WATER. WATER STRUCTURAL MEMORY OF WATER. WATER ABILITY TO INFORMATION ACCUMULATIONABILITY TO INFORMATION ACCUMULATION APPENDIX 2

3.3. OPISTHORCHOSISOPISTHORCHOSISAPPENDIX 3

4.4. VACCINATION FROM A TUBERCULOSIS BY CEM VACCINATION FROM A TUBERCULOSIS BY CEM TECHNOLOGY. TECHNOLOGY. EXPERIMENTS ON ANIMALS (MICE)EXPERIMENTS ON ANIMALS (MICE)APPENDIX 4

Appendices

APPENDIX 1

We used our measuring technique for research of homeopathic preparations. These researches showed that the applied technique of measurements made it possible to determine homeopathic preparations in degrees of dilution up to 10-6000 M. The opportunity of information transfer of activity of homeopathic preparations was also achieved. We used also this measuring technique for estimation of quality of water activation by different methods. Below you can see the diagrams, which characterize dynamic condition of water after electrochemical activation. Distinction of the water, received on anode and on cathode and at different current value, is shown. The controlled energy materials, received at transfer of EM-activity (for example, from ”guest” microorganism onto material), are as easily controlled with the above fluctiation light-scattering technique as at work with homeopathic preparations.

Control Device for CEM Recording

The derivations of light- scattering fluctuation spectrum in 1 - distilled water 2 - EchA water 3 - EchA water with transferred preparation activity I - spectral density of capacity (relative units) W - frequency (relative units)

Transfer of material with modified structure by microwave device into water

APPENDIX 1 Control Device for CEM Recording

The derivations of light-scattering fluctuation spectrum in1 - distilled water 2 - preparation transferred through glass3 - preparation transferred direct in water I - spectral density of capacity (relative units) W - frequency (relative units)

Transfer of preparation activity (Echinacea) by microwave device in the water


The derivations of light scattering fluctuation spectrum in1 - ethanol 27% 2 - initial homeopathic preparation (EDAS 108) 3 - homeopathic preparation (EDAS 108) by 10-times transfused through MF 10 E

(it is equal to ethanol)4 - homeopathic preparation (EDAS 108) by 11-times transfused through MF 10 E

(it is equal to ethanol)I - spectral density of capacity (relative units) W - frequency (relative units.)

Removing of homeopathic preparation activityby magnetic field treatment


The derivations of light scattering fluctuation spectrum in1 - distilled water 2 - preparation transferred through glass 3 - preparation transferred direct in water I - spectral density of capacity (relative units) W - frequency (relative units)

Transfer of preparation activity (Nitroglycerin)by microwave device in the water


STRUCTURAL MEMORY OF THE WATERSTRUCTURAL MEMORY OF THE WATER1. The crystal of distilled water which were not subjected

to any influence1. Spring water2. The Antarctic ice3. The crystal of the water, which have heard

"Pastoral" by Beethoven

5. The crystal formed after listening of heavy metal music6. The crystal after influence of words "you are a fool",

is very similar to the crystal formed after action of heavy metal music

5. Word "Angel"6. Word "Devil"

9. Water has received the request “Make it "10. Water has received the order “Make it "11. Words " I’m tired of you. I shall kill you "12. Water received electromagnetic radiations

of love and gratitude

APPENDIX 2

Structure of the Water

13. Sample of water water from Shinagawa, Tokyo14. The same sample after 500 instructors of HADO from all over

Japan have simultaneously sent kind thoughts to it.15. The water taken from lake Fujiwara, before a pray.16. Crystal of water after the pray of buddhist priest Kato

17. Words " Love and gratitude ", said in English18. Words " Love and gratitude ", said in the Japanese language19. Words " Love and gratitude ", said in German

20. Left: a camomile,right: its respective in crystallization of water21. Left: dill, right: its respective in crystallization of water

APPENDIX 2

Structure of the Water

Dynamics of morphological changes in the liverDynamics of morphological changes in the liverat different variants of opisthorchosis therapyat different variants of opisthorchosis therapy

SIBERIAN STATE MEDICAL UNIVERSITY2002

APPENDIX 3

The purpose of research:

To establish character and dynamics of morphological changes in the liver at different variants of therapy for opisthorchosis.

Opisthorchosis Experiment

APPENDIX 3

Research problems:

1. To estimate the character of histological changes in the liver of hamsters at opisthorchosis before treatment and after treatment.

2. To make comparative morphological analysis of necrotic changes

in the liver between researched groups.

3. To study character and dynamics of inflammatory infiltration of portal tracts in the given groups.

4. To estimate character and dynamics of fibrosis in the liver in researched groups.

5. To estimate dynamics of histologic changes as a whole in the liver of hamsters at different variants of opisthorchosis therapy.


APPENDIX 3

Materials and methods:

The complex comparative morphological analysis of the liver was conducted in the following groups. The control group consisted of 20 fragments of the liver. Group № 1 is tissue of liver after treatment with aekarsol (specific drug). Group № 2 is tissue of the liver after treatment with electromagnetic (EM)

analogue of opisthorchisis. Group № 3 tissue of the liver after treatment with electromagnetic (EM)

analogue of aekarsol. Fragments of the liver after sampling were immediately fixed in 12 % neutral formalin. The preparations were made in accordance with a standard technique, they were covered with paraffin. Cuts 5-6 microns thick were painted with eosin-hematoxylin, picrofuchsin by Van Gizon.


APPENDIX 3

Necrosis-inflammatory changes and character of fibrosis were estimated.Necrosis and inflammatory infiltration were referred to necrosis-inflammatory. The following variants of necrosis were taken into account: piecemeal necrosis, bridging necrosis and intra-lobular n ecrosis. Qualitative (eosinophiles, neutrophils, lymphocytes, monocytes, fibroblasts) and quantitative calculation of cellular infiltration of portal tracts, and also density of infiltration in 1 mm2 were made. The following variants of fibrosis were marked out and taken into account: portal, septal, (porto-portal, porto-central). Using function “lasso” in PhotoShop, they calculated the area of portal tracts, number of cells in infiltration per unit area with subsequent calculation of specific weight of each cellular form. For unification of research within the limits of each micropreparation the estimation was carried out in ten fields of each third vision field. Four groups of liver fragments, including control one, “blind” research was performed. The morphological assessment was on the device, which included light microscope “Zeiss Jena”, digital camera Epson-200 with the use of program PhotoShop 6.0. The image of vision field was introduced into computer with the help of videocamera. The final linear and optical increase after transfer of via videocamera was х800, 1600.


APPENDIX 3

Results and discussion

On comparing assessment results of necrotic changes in the liver, the following characteristics were revealed. The therapy with the use of aekarsol (specific drug), EM analogue of aekarsol and EM analogue of opisthorchisis was accompanied by positive dynamics. So, if in the control group, the number of piecemeal, bridging and intra-lobular necroses in 10 vision fields was accordingly - 16, 6, 10, at the same time, in the group, treated with aekarsol1 it was 4, 2, 6 (Р≤0,05k), and in the group with use of EM analogue of aekarsol2 - 9, 3, 6 (Р≤0,05k), in the group with application of EM analogue of opisthorchisis 3 - 9, 3, 5 (Р≤0,05k) (See Table 1).


APPENDIX 3

Table 1Occurrence of different variants of necrosis in the liver in researched groups

(“n” in 10 vision fields under increase of 80)

Group Piecemeal necrosis

Bridging necrosis

Intra-lobular necrosis

Control 16 6 10 Aekarsol 4 2 6 EM analogue of opisthorchisis

9 3 5

EM analogue of aekarsol

9 3 6


APPENDIX 3

On comparing parameters of cellular infiltration of portal tracts among different groups, the following differences were also revealed. In control preparations the number of cells per 1 мм2 amounted to 14500 ± 1800, whereas in groups 1, 2, and 3 the number of cells was 6140 ±810, 8200±980, 8570 ±1050 per unit area accordingly, which was statistically different (see Table 2). At comparative assessment of percentage of each cellular form of inflammatory infiltration the following characteristics were revealed. If in the control group specific weight of eosinophilic leucocytes amounted to 48 ±6, in group 1 it was 12 % ±2 (Р≤0,05k),in group 2 - 23 ±%3 (Р≤0,05k), and in group 3 - 13 % ± 2 (Р≤0,05k). Thus in the control group the content of lymphocytes was 20 % ±3, whereas in group 1 it was 34 % ±4, in group 2 - 40 % ±5, and in group 3 - 42 % ±6. Distinctions with control group are reliable. In comparison with the control group, multiple reduction of neutrophil specific weight in all groups was observed after treatment, upon authentic sharp increase of number of monocytes (see Table 2).


APPENDIX 3

Table 2Structure of inflammatory infiltration of portal tracts in researched groups

(comparison in % ± M)

Group Cell elements Density per 1mm

Eosino-philes

Neutro-phils

Fibro-blasts

Lympho-cytes

Mono-cytes

Control 48±6 12±1,8 3±0,4 20±3 17±2,5 1450±1800 Aekarsol 12±2 4±0,5 2±0,2 34±4 48±7 6140±810 EM analogue of opisthor-chisis

23±3 6±0,9 3±0,5 40±5 28±3,8 8200±980


13±2 6±0,8 6±0,7 42±6 33±4,2 8500±1050


APPENDIX 3

Comparative assessment of fibrosis character revealed that in groups 1- 3 in comparison with the control groupsclerosis evidence was authentically less.

Thus, if in the control group the number of porto-portal and porto-central septa in 10 vision fields was 6 to 8,in group 1 it was 2 to 0,in group 2 it was 3 to 1,in group 3 it made 3 to 3.

Reliability of distinctions is confirmed statistically (Р≤0,05) (see Table 3)


APPENDIX 3

Table 3

Frequency of occurrence of different fibrosis variants in researched groups(“n” in vision fields under increase of 80)

Group Fibrosis variants Portal Porto-portal Porto-central Control 20 6 8 EM analogue of opisthorchisis

8 3 1


8 2 3

Aekarsol 6 2 0


APPENDIX 3

During the carried out research we revealed that therapy for opisthorchosis with use of EM analogue of opisthorchisis and aekarsol is accompanied by authentic positive dynamics of morphological picture, which is shown by reduction of inflammatory infiltration density, obvious decrease of necrotic changes and extremely weak fibrosis evidence (in comparison with control group). Application of EM analogues does not cause frequently observed sclero-gene effect, which is very valuable quality of the preparations.


APPENDIX 3

Thus, both aekarsol therapy and therapy with use of EM analogue of aekarsol and opisthorchisis, are accompanied by positive morphological dynamics, significant reduction of not only necrotic and inflammatory changes, but also of sclerous ones.


VACCINATION FROM TUBERCULOSIS VACCINATION FROM TUBERCULOSIS BY CEM TECHNOLOGY.BY CEM TECHNOLOGY.

EXPERIMENTS ON ANIMALS (MICE)EXPERIMENTS ON ANIMALS (MICE)

APPENDIX 4

CEM Vaccination

APPENDIX 4

The ability for vaccinating living organisms from tuberculosis by electron analog (CEM Technology) of vaccinal culture Micobacterium bovis (BCG) was checked experimentally with 3 groups of mice. First group of 20 mice of the inbreed line C57BL/6 was vaccinated by subcutaneous introduction 2 10 5 alive culture of avirulent vaccinal culture Micobacterium bovis (BCG). Capsules-carriers with preliminary recorded signals from culture BCG, were placed on each mouse in the second group of 20 mice. Third group of 20 mice was control group. In 5 weeks first and second groups of mice, and also third control group were infected with intravenous introduction virulent M.tuberculosis (MBT) culture H37Rv in a doze of 5 10 6. Before infection, mice of the first and second groups were put to the tuberculine tests: tuberculine was injected into small pillows of the left hinder legs and in 24 hours the difference of thickness left and right (control) small pillows was estimated. It was revealed that BCG vaccinated mice had the value of hypersensitivity of slowed down type (HST) equal to 0.17 ± 0.04 mm, and in the second group it was 0.15 ± 0.08 mm. Both values are significant and do not differ authentically from each other, which testifies that the prepared electron carrier is capable “to make” mice immunity similar to BCG.

CEM Vaccination

APPENDIX 4

Organs of infected mice in the first and the second groups (10 mice from each group) were taken for histologic researches in 1 month and 3 months after infection. Within 3 months all mice of the first two groups remained alive (10 mice in each group), and in the control group during the first month 12 mice were lost, in 3 months - others died, which testifies that effect of vaccination was achieved in the first two groups. Histologic researches have shown the following: in the control group the process of disease was characterized by generalization with damage of lungs, liver and spleen. In the lungs the overall specific pneumonia was found out as large, merging among themselves, infiltrations with numerous, breaking apart, neutrophiles, macrophages, lymphocytes, epithelioid cells, plethora vessels and hypostases. Air pulmonary tissue, remained between injured sites, made 10-15 % from total volume of the lung. The mice of the first group, infected with MBT, which were BCG vaccinated 5 weeks prior to the infection, in 1 month after infection immune tissue reorganization was detected only as proliferation of lymphoid and reticular cells in the spleen.

CEM Vaccination

APPENDIX 4

Small proliferation of endotheliacytes was also found out in the liver. Plethoric capillary network and small diffusive lymphoid and macrophage infiltration of intersticium was found out in the lungs. Mice of the same group in 3 months after infection had immunocyte proliferation already sharply expressed, the spleen increased in 10 and more times. in comparison with the normal one. Follicles were large, numerous; T- lymphocyte proliferation appeared, endotheliacytes grew in number, compared to the previous supervision. Lymphoid focal congestions appeared in the lungs. Air pulmonary tissue, in relation to the affected tissue, made 80 % of the total volume.

CEM Vaccination

APPENDIX 4

The mice, infected with MBT, which 5 weeks prior to the infection and during all the experience were receiving influence of the capsule-carrier with recorded BCG spectrum, had changes in organs similar to the mice, vaccinated by the injection.The tuberculine tests analysis and study of morphological material have shown, that mice of the first and the second groups had identical organism reaction to MBT infection. It was completely different from obzerved organ changes of non-vaccinated animals. Moreover, mice of the first and the second groups had vivid differences neither on the degree of immune reorganization, nor on the morphological picture in the internal organs. It means that both vaccination methods are identical in their protective action. These effects are illustrated with the photos in Fig. 4.1, 4.2, 4.3. Fig. 4.1 shows lung of the control mouse in 1 month after infection, total specific pneumonia is observed. In Fig. 4.2 - lung of the mouse, vaccinated with the help of injection in 3 months after infection, in Fig. 4.3 - mouse lung, vaccinated by the capsule–carrier in 3 months after infection. There is absence of typical tubercular centers in both cases.

CEM Vaccination

Fig. 1 Lung of the control mouse in 1 month after infection with MBT (microbacterium of tuberculosis). Total specific pneumonia. Absence of air pulmonary tissue. Hematoxylin and eosin stain. Enlargement х170.

APPENDIX 4

CEM Vaccin

ation

Fig. 2 Lung of the mouse in 3 months after infection with MBT, preliminary vaccinated with BCG. Lymphoid-macrophage foci in subpleural zone

of the lung. Absence of typical tubercular lesions. Hematoxylin and eosin stain. Enlargement х170.

APPENDIX 4

CEM Vaccination

Fig. 3 Lung of the mouse in 3 months after infection with MBT, with preliminary treating according to CEM Technology. Sites of lymphoid-macrophage infiltration on the background of emphysema. Absence of typical tubercular foci. Hematoxylin and eosin stain. Enlargement х170.

APPENDIX 4

CEM Vaccination

The project is the winner of the Foundation for Assistance to Small Innovative Enterprises (FASIE) set up by Russian Federation government Development.

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