Capturem ™ Antibody Purification www.takara.co.kr • High-capacity Protein A membranes • 신속한 항체 스크리닝 (antibody screening)에 최적 • 효율적인 IP & Co-IP (Immunoprecipitation) 실험 가능 • 배지 교체 (media exchange) 불필요 ☐ Save Time ☐ High Quality ☐ High Purity ☐ No Contamination 실온에서 5분이면 항체 정제 완료 항체의 응집/실활을 방지하여 고품질의 항체 정제 오염 물질이 거의 없는 고순도 항체 정제 정제 과정 중의 오염 또는 carryover 방지 대~박! 항체를 DNA처럼 Miniprep Kit로 정제? Capturem ™ Protein A Miniprep Columns Capturem ™ Protein A Miniprep (Code 635717) Capturem ™ Protein A Rapid Antibody Purification The Next Generation of Antibody Purification 실온에서 5분이면 항체 정제 완료! 그림 1. Capturem ™ membrane technology for Protein A purification Modified membrane pores 그림 2. Animal sera antibody binding test 다양한 동물 유래의 혈청 (animal sera)으로부터 Capturem ™ Protein A Miniprep Spin Column을 이용하여 고순도의 항체를 정제할 수 있었다. Marker Sheep Goat Rat Mouse Human Rabbit Horse * Sample: animal sera, ascites fluid, cell culture media 등 * Compatible with all standard buffers * 정제수율: 300 ㎍ / column (binding capacity: ≥ 75 mg/㎖) * 정제시간: 5분 (~15분) 샘플 가능 Animal sera Sheep Goat Rat Mouse Human Rabbit Horse Purified antibody (㎍) 256 184 170 140 129 102 215
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The Next Generation of Antibody Purificationcms.takara.co.kr/file/brochure/capturem_bro.pdfantibody purification methods: • Purification at neutral pH—avoid antibody aggregates
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C a p t u r e m ™ A n t i b o d y P u r i f i c a t i o n
www.takara.co.kr
• High-capacity Protein A membranes
• 신속한 항체 스크리닝 (antibody screening)에 최적
• 효율적인 IP & Co-IP (Immunoprecipitation) 실험 가능
• 배지 교체 (media exchange) 불필요
☐ Save Time
☐ High Quality
☐ High Purity
☐ No Contamination
실온에서 5분이면 항체 정제 완료
항체의 응집/실활을 방지하여 고품질의 항체 정제
오염 물질이 거의 없는 고순도 항체 정제
정제 과정 중의 오염 또는 carryover 방지
대~박! 항체를 DNA처럼 Miniprep Kit로 정제?
Capturem™ Protein A Miniprep Columns
Capturem™ Protein A Miniprep (Code 635717)
Capturem™ Protein ARapid Antibody Purification
The Next Generation of Antibody Purification
실온에서 5분이면 항체 정제 완료!
그림 1. Capturem™ membrane technology for Protein A purification
Modified membrane pores
그림 2. Animal sera antibody binding test
다양한 동물 유래의 혈청 (animal sera)으로부터 Capturem™
Protein A Miniprep Spin Column을 이용하여 고순도의 항체를
정제할 수 있었다.
Marke
rSh
eep
Goat
Rat
Mou
seHu
man
Rabb
itHo
rse
* Sample: animal sera, ascites fluid, cell culture media 등
His-Tagged Protein Purification – column 형태의 His-Tagged 단백질 정제
635717
635716
12 Purifications
96 Purifications
12개의 disposable spin columns 포함
96well plate의 high-throughput system
635710
635713
635714
635719
20 Purifications
6 Purifications
96 Purifications
50 Columns
100 ㎍ (농도 0.3 ~ 1 mg/㎖) 단백질 정제
2.5 mg (농도 4.5 mg/㎖) 단백질 정제
96well plate의 high-throughput system
Maxiprep column만 별도 구매
• Capturem™으로 양호한 단백질 발현 및 정제를 확인했다면?
• 보다 많은 양의 large scale 단백질 정제를 원한다면?
at room temperture
minutes!Only 5
Clontechonly
A Takara Bio Company
www.clontech.com
Clontech Laboratories, Inc.
P r o t e i n e x P r e s s i o n & P u r i f i c a t i o n
IgY IgM
IgG IgE
Broader selectivity
Thiophilic Antibody Purification Resins
An efficient, versatile, and economical alternative to Protein A purification
Clontech’s Thiophilic-Uniflow and -Superflow Resins provide highly stable
purified antibodies with the following advantages over conventional Protein A
antibody purification methods:
• Purification at neutral pH—avoid antibody aggregates
• High capacity (20–25 mg Ab/ml resin)—get high recoveries
• Broader selectivity—purify IgY, IgM, IgE, and scAb
• Fewer purification steps—save time
• Reusable resin—save money
• Higher stability of the purified product
Protein A vs. Thiophilic Resin
Historically, Protein A has been the preferred method of immunoglobulin purification. However, there are certain types of antibodies, such as the single-chain antibodies IgE, IgY, and IgM, that cannot be purified using Protein A. Thiophilic adsorption chromatography is ideal for these types of applications, as well as immunoglobulin purification in general.
In addition to providing broader selectivity and higher stability, thiophilic adsorp-tion chromatography offers a faster protocol than Protein A under neutral buffer conditions (Figure 1). Purification with Thiophilic Resin allows elution in any buffer with pH 7.0. In contrast, Protein A requires elution in a buffer with pH lower than 7.0. Protein A also requires a dialysis step, which can be omitted with Thiophilic Resin.
Figure 1. Thiophilic Resin purifies antibodies at neutral pH and in less time than Protein A.