The Measurable Difference Prepared for U.S. Aim High Conferences, 2004.

The Measurable Difference

Prepared for U.S. Aim High Conferences, 2004

What is LifePak®?

A comprehensive nutritional wellness program delivering the optimum amounts of all essential and generally beneficial nutrients for long-term health and well-being.*

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Comprehensive & Convenient

>Multi

VitaminVitamin E

300 IU

Calcium &Magnesium

Chromium200 mcg

MultiCarotenes

Vitamin C500 mg

MultiFlavonoid

Better than these 7 popular supplements together.

LifePak® Health BenefitsOptimum supplementation for general well-being*1. Addresses nutrient deficiencies*2. Cardiovascular benefits*3. Complete bone nutrition*4. Promotes healthy immune function*5. Supports normal blood sugar metabolism*6. Protects against the symptoms of aging*

7. Raises your antioxidant defense**These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

The Need to Supplement

“… low levels of the antioxidant vitamins (vitamins A, E, and C) may increase risk for several chronic diseases. Most people do not consume an optimal amount of all vitamins by diet alone.”

“It appears prudent for all adults to take vitamin supplements.”

Journal of the American Medical Association June 2002

Robert H. Fletcher, MD, MSc; Kathleen M. Fairfield, MD, DrPH, Harvard UniversityJournal of the American Medical Association 2002; 287: 3127-3129

A Landmark Endorsement

Free Radicals• Highly reactive, short-lived molecules• Damage cells and genetic material (DNA)

• 70,000 free radical attacks on DNA every day in a cell• Aging process• Implicated in most health conditions• Many different types of free radicals

Free Radicals & Antioxidants

Sources of Free Radicals

• Environmental pollution (smog, ozone, NO2) • Radiation exposure:

– sunlight (UV-light), X-rays, etc.• Smoking:

– 10,000,000,000,000,000 free radicals/cigarette!

• Metabolism in your mitochondria

Antioxidants fight free radicals• Slow down aging process & improve health:

• over 30,000 research studies on antioxidant health benefits• affecting over 50 health conditions, including cardiovascular

function, eye health, CNS health, skin health, etc.

• Many antioxidants work together: “Antioxidant Defense System”

• Relies on a steady supply of dietary antioxidants

Free Radicals & Antioxidants

LifePak® 40+ Antioxidants

• All 8 forms of natural vitamin E: 300 IU• Buffered vitamin C: 500 mg• Selenium, Copper, Zinc, Manganese• 6 Carotenoids:

– as found in 5-10 fruits & vegetables per day • Over 20 Flavonoids:

– 6 Catechins, Quercetin, naringenin, hesperidin, 3 Soy isoflavones, Over 10 grape seed polyphenols

• Alpha-Lipoic Acid: 30-50 mg

LifePak® Science is Measurable

• First to show antioxidant and cardiovascular benefits in double-blind studies*

• 200,000+ published studies on LifePak’s antioxidants

• Nearly 800,000 published studies on all LifePak ingredients

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

LifePak® Clinical StudyAntioxidant and Cardiovascular Health*

42.1 42

43.4

50.9

30

35

40

45

50

55

60

65

Baseline Endpoint

PlaceboLifePak

p < 0.001

331299

335

716

0

200

400

600

800

1000

1200

Baseline Endpoint

PlaceboLifePak

p < 0.001

3.4773.429

6.248

3.305

0

1

2

3

4

5

6

7

8

Baseline Endpoint

PlaceboLifePak

p < 0.001

Smidt CR, Seidehamel RJ, Devaraj S, Jialal I. FASEB Journal Vol.13 No.4, p.A546, March 1999.

LDL Protection*

Vitamin E

Beta-carotene

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

LifePak® Antioxidant Capacity (ORAC)Jan 2003

0

500

1000

1500

2000

2500

3000

3500

MM

-1

MLM

-A

MLM

-B

MLM

-CM

M-2

MLM

-D

MLM

-E

LifePak

Pharmanex® BioPhotonic Scanner

• LifePak® augments the entire antioxidant defense system*

• The effect can easily be demonstrated using the BioPhotonic Scanner

• Clinical studies identify link between scores and healthy diet and lifestyle

*This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Function and Structure of Carotenoids

• uptake through the diet• safe vitamin A sources (a- & b-carotene)• antioxidants (1O2 quenchers)• promote immune function (b-carotene)• protect cellular DNA (lycopene)• protect macular region of retina (lutein)

Lycopene

-carotene

-carotene

Zeaxanthin

Lutein

Biological Functions of Carotenoids

Color of the beak is signal female zebra finches consider when selecting a mate; clear now that a male's carotenoid levels, and therefore attractiveness, are linked to the effectiveness of his immune function.

Bright beaks in birds signal health of the immune system

Source: Pennisi, E. 2003. Colorful males flaunt their health. Science 300: 29-30

Zebra finch

SpectrometerAn instrument that separates light signals into different frequencies, producing a spectrum

What is a Spectrometer?

Vibronic Energy Levels

Laser light

Raman scattered light

Excited Carotenoid molecules shift blue laser light color from blue to green

What is Raman Spectroscopy?

“Raman” effectA scattering of electromagnetic radiation through a transparent medium in which light changes in frequency (color)

Scanner Science is Measurable• National Institutes of Health and Office of Dietary Supplements• New York Academy of Sciences• Oxygen Club of California• FASEB/American Physiological Society - Oct 2003 and April

2004• Gordon Research Conference• Review paper pending publication in Current Topics in

Nutraceutical Research

Demographic Study - Sep 2002

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100

120

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7000

1000

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1300

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2200

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BDS Categories

Fre

qu

ency 1st

5th10th

25th50th

75th90th 95th 99th

Percentile Rankings: 1st 4,500 5th 8,500 10th 10,500 25th 13,500 50th 17,500 75th 22,500 90th 30,500 95th 36,500 99th 48,000

Mean: 19,072SD: 8,828

1,375 Subjects

1,375-Subject Study

Results show that Skin Carotenoid Signal:

• Is not influenced by Age, gender or skin pigmentation (race/ethnicity)

• Is high in people who eat more fruits & vegetables

• Is high in people who take LifePak*• Is low in people who smoke or have

oxidative stress (free radical damage)

Demographic Study

16827

19669

2537723997

0

5000

10000

15000

20000

25000

30000

1 or Less 2 to 3 4 to 5 6 or More

Reported Daily Fruit & Vegetable Servings

Ram

an

In

ten

sit

y, C

ou

nts

.

n = 534 n = 649 n = 137 n = 30

p < 0.01 p < 0.01

17888

20193

25717

28848

0

5000

10000

15000

20000

25000

30000

35000

None Irregular One/Day Two/Day

Reported LifePak Usage

Ram

an

In

ten

sit

y, C

ou

nts

.

n = 59n = 89n = 192n = 800

p < 0.01

p < 0.01

*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Clinical Study - Sep 2002 LifePak® Scanner Score*

32175

25991

28924

18828

10000

15000

20000

25000

30000

35000

40000

0 4 8 12Time (Weeks)

Ram

an

In

ten

sit

y, C

ou

nts

.

p < 0.001

p < 0.01

p = 0.23

*This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Body Fat - Inverse Correlation

0

5000

10000

15000

20000

25000

30000

Normal Overweight Obese Morbidly Obese

Body Mass Index (BMI)

Ra

ma

n I

nte

ns

ity

(m

ea

n)

(N = 131,122 scans)

Antioxidant Status and Weight Loss

1. Overweight and obesity are associated with decreased antioxidant protection.

2. Dieting causes stress to the body, generating more free radicals, which may result in decreased antioxidant protection.

ConclusionOverweight individuals and anyone on a diet should increase antioxidant protection through diet and supplementation.

Carotenoid Correlation Study June 2003

0

10,000

20,000

30,000

40,000

50,000

60,000

0.0 1.0 2.0 3.0 4.0

Total Serum Carotenoids (mg/ml)

Ra

ma

n In

ten

sit

y C

ou

nts

r = 0.78 p < 0.001

Pending publication, FASEB April 2004

Scan Values in Asians Singapore study - Dec 2003 Thailand study - Jan 2004 Same data relationship as U.S.

study (diet, supplements, body fat, lifestyle)

25,000 average with 82% of participants taking LifePak®

LifePak® users (2/day) scored 25% higher than non-LP users*

20,000

22,000

24,000

26,000

28,000

LifePak 2/dayLifePak 1/dayno Lifepak

Internal data on file. *This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Summary of Scanner Research

Data from 300,000 scans and Asian studies correlate with original demographic study of 1,300 participants

Known risk factors that induce oxidative stress reduce scanner score

Healthy diets improves score Long-term LifePak® supplementation improves score* Score is related to lean body mass & body fat Desirable score is the blue zone, the higher the better

*This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Media Coverage in 2003Self Magazine April 03

Elle Magazine Sep 03

Ms. Fitness Magazine Oct 03

NBC Today in LA

KNXV ABC News

Phoenix AZ News 3

WHDH-TV NBC Boston MA

WHAS 11 News Louisville KY

News All Morning New York

KUTV News Salt Lake City

NewsChannel 4 Columbus OH

CNBC Asia - Singapore

The QuestionsHow Does Measuring Skin Carotenoids Predict Antioxidant Status?

VITAMIN CcycleVITAMIN E

cycle

THIOLcycle

NAD(P)H + H+

NAD(P)+

Lipid–water interface

ROOHROH

PUFA

O2– and other radicals

Metabolism, strenuous exercise,cigarette smoke, pollution

ROO•RO•

The Antioxidant Network

Carotenoids

The QuestionsWhat Does “25,000” Mean?

16827

19669

2537723997

0

5000

10000

15000

20000

25000

30000

1 or Less 2 to 3 4 to 5 6 or More

Fruit & Vegetable Servings

Skin

Caro

ten

oid

Sco

re

n = 534 n = 649 n = 137 n = 30

p < 0.01 p < 0.01

• Serum equivalent ~ 1 mcg/ml (+ 10%, 95% CI)

1000 units ~ 0.04 mcg/ml• ~ 5 servings fruits/veg. per day

Average Diet• 2-3 fruits/veg.• Scan 19,000 • 0.7 mcg/ml serum

How Can I Improve My Score?

10,000

20,000

30,000

40,000

50,000

High

Moderate to High

Moderate

Low

• Moderate fruit/veg. intake

• Irregular supplement use

• Smoker

• High body fat

(i.e. s

moking)

SCS = 20,000

The QuestionsHow Can I Improve My Score?

10,000

20,000

30,000

40,000

50,000

High

Moderate to High

Moderate

Low

SCS = 42,000

• Take LifePak® 2x/day

• Improve diet

• Quit smoking

• Lose weight

(i.e. s

moking)

Live Better, Longer

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LifePak® Family

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Optimal Health

Free Radical Protection - Pharmanex® BioPhotonic Scanner