The inhibitory signaling receptor protocadherin-18 regulates … · 2017. 9. 2. · memory T cells (CD8 + CD44 + CD62L + CD127. hi), is not expressed in B cells, NK cells, naive CD8
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1
The inhibitory signaling receptor protocadherin-18 regulates tumor-infiltrating CD8+ T cell
function
Alan B. Frey
Department of Cell Biology and Perlmutter Cancer Center New York University Langone School of Medicine 550 First Avenue, New York, NY 10016 USA Correspondence: [email protected] Tel: (212) 263-8129; FAX: (212) 263-8139 Conflict of Interest Statement: The author declares no potential conflicts of interest. Acknowledgements: Research in the author’s lab was supported by NIH R01CA108573 and Pfizer Inc (through the CTI program), both to ABF, and to NYULSM for core facilities support (P30CA016087 for Cytometry and Cell Sorting, Histopathology, and Genome Technology).
Running title: Protocadherin-18 regulates TIL function
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
with OKT3 (2ug/mL) with or without a rabbit IgG anti-pcdh18, anti-PD-1, or control IgG of
appropriate species. Cell number data (C) is representative of three experiments.
Supernatants of cells in C were collected for IFN ELISA (in D) and is representative of
two experiments.
Citations
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Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
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Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187
Published OnlineFirst September 5, 2017.Cancer Immunol Res Alan B. Frey tumor infiltrating CD8+ T cell functionThe inhibitory signaling receptor protocadherin-18 regulates
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Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on September 5, 2017; DOI: 10.1158/2326-6066.CIR-17-0187