The eukaryotic cell cycle
The eukaryotic cell cycle
Mitosis and cytokinesis
Blue-DNA Green-microtubule
Fig. 19-36
Mitotic apparatus of mammalian cells
Centrosome duplication and cell cycle
Microtubule motors and centrosome movement at prophase
Fig. 19-43
Capture chromosomesand stabilizing
chromosomes at the equatorial plate in metaphase
Shortening at the (+) end of kinetochore microtubules
moves chromosomes poleward in anaphase A
Spindle elongation and movement of poles during anaphase B
Whether does the spindle or the asters control location of the cleavage plane during cytokinesis?
Regulation of myosin during cytokinesis
MPF-mitosis promoting factor
Mitosis in a higher plant cell
Regulation of cell cycle
Protein factors
Cyclin
CDK-cyclin-dependent kinase
Cyclin complex G1 cyclin-CDK complexesS phase cyclin-CDK complexMitotic cyclin-CDK complex
Cell cycle inhibitors
Cell cycle promoting factor -ubiquitin ligase lead to degradation of cell cycle inhibitors
SCF and APC
Mechanisms
Phosphorylation and dephosphorylation
Activation of gene transcription and translation
Proteasome-mediated degradation
Current model for regulation of the
eukaryotic cell cycle
CDK-cyclin-dependent kinaseSCF-ubiquitin ligase
APC-anaphase promoting complex
A diffusible factor in arrested Xenopus eggs promotes meiotic maturation
Biochemical detection of cyclical synthesis and destruction of mitotic cyclin in sea urchin embryos
Isolation of cell-division cycle (CDC) genes from a S. cerevisiaegenomic library by functional complementation of cdc mutants
Using S. pombe cdc mutants to study the cell cycle regulation
Regulation of the kinase activity of S. pombe mitosis-
promoting factor by phosphorylation and dephosphorylation
Depolymerization of the nuclear lamina
The phosphorylation of human lamin a is required for lamin depolymerization
Control of entry into anaphase by APC-regulated degradation of the cohesin link between sister chromatides
The reassembly of nuclear envelop during telophase
Mammalian cyclin-CDK complexes
Using bromodeoxyuridin (BrdU) to test the function of cyclin D in mammalian cells
Checkpoint controls in cell cycle
Role of p53 in cell cycle check points