The Effects of Pollution on Bioluminescent Bacteria By: Tobi Simon.

The Effects of Pollution The Effects of Pollution on Bioluminescent on Bioluminescent

BacteriaBacteriaBy: Tobi SimonBy: Tobi Simon

NeedNeed

Long-term exposureLong-term exposure Plants and Animals Plants and Animals

http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png

Knowledge BaseKnowledge Base

http://nptel.iitm.ac.in/courses/Webcourse-contents/IIT-Delhi/Environmental%20Air%20Pollution/air%20pollution%20(Civil)/Module-1/images1/35.gif

Knowledge BaseKnowledge Base

http://www.mcbs.unh.edu/faculty/Whistler/images/image3.jpg

Bioluminescent Bacteria

Literature ReviewLiterature Review

Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." in Saturated Porous Media." Journal of Environmental QualityJournal of Environmental Quality (2006).(2006).

Literature Review

Alvarez, David A. "Chemical and Toxicologic Assessment of Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Organic Contaminants in Surface Water Using Passive Samples." Samples." Journal of Environmental QualityJournal of Environmental Quality (2008). (2008).

Literature Review

Vanhaecke, Erwin. "Bioluminescence Assay for Measuring Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." in the BATH Test." Applied and Environmental MicrobiologyApplied and Environmental Microbiology (1988).(1988).

Literature Review

Frischer, M. E. "Bioluminescent Bacteria as Indicators of Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Chemical Contamination of Coastal Waters." Journal of Journal of Environmental QualityEnvironmental Quality (2005). (2005).

PurposePurpose

The effects of pollution on the amount The effects of pollution on the amount of light emitted from bioluminescent of light emitted from bioluminescent bacteria.bacteria.

HypothesisHypothesis

Alternate: Water with higher amounts Alternate: Water with higher amounts of hydrocarbons will cause a of hydrocarbons will cause a decrease in the amount of light decrease in the amount of light emitted from the bacteriaemitted from the bacteria

Null: Water with hydrocarbons in it will Null: Water with hydrocarbons in it will have no effect on the amount of light have no effect on the amount of light emitted from the bacteria. emitted from the bacteria.

MethodologyMethodology

Water is collected from different locations and a sample of each is tested for the amount of hydrocarbons present.Bioluminescent bacteria are placed into different samples of water

After 24 hours the BLR will be calculated for each sample of water.

ControlNo hydrocarbon in water

Variable 1Lowest amount of hydrocarbon in water

Variable 2Medium amount of hydrocarbon in water

Variable 3Highest amount of hydrocarbon in water

Statistical Analysis: ANOVA followed by a Sheffe post hoc test

Do AbilityDo Ability

OrganismsOrganisms ProceduresProcedures

http://www.abdn.ac.uk/staffpages/uploads/bch052/aglover-bac.jpg

BudgetBudget

Vendor Order # Quantity Description PriceTotal Price

Sigma F7911-100EA 1 Millipore glass-fiber filters 86.7 86.7

Sigma A7002-250G 1 Agar 63 63

Sigma Peptone

Sigma S9148-1L 10 Seawater 20.7 207

Sigma Y1626-250G 1 Yeast Extract 115 115

Incubator

Spectrophotometer

Sigma P5481-500EA 1 Petri dishes 83.1 83.1

Carolina Science NP-15-5723 3 Vibrio fischeri 10.95 32.85

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