Immunity, Volume 44 Supplemental Information The DNA Structure-Specific Endonuclease MUS81 Mediates DNA Sensor STING-Dependent Host Rejection of Prostate Cancer Cells Samantha S.W. Ho, Wendy Y.L. Zhang, Nikki Yi Jie Tan, Muznah Khatoo, Manuel A. Suter, Shubhita Tripathi, Florence S.G. Cheung, Weng Khong Lim, Puay Hoon Tan, Joanne Ngeow, and Stephan Gasser
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Immunity, Volume 44
Supplemental Information
The DNA Structure-Specific Endonuclease
MUS81 Mediates DNA Sensor STING-Dependent
Host Rejection of Prostate Cancer Cells
Samantha S.W. Ho, Wendy Y.L. Zhang, Nikki Yi Jie Tan, Muznah Khatoo, Manuel A.Suter, Shubhita Tripathi, Florence S.G. Cheung, Weng Khong Lim, Puay HoonTan, Joanne Ngeow, and Stephan Gasser
cells transduced with control (white column) or Mus81 (grey column)-specific
shRNA were examined by flow cytometry to determine BrdU incorporation and
DNA content (7-AAD staining). Shown are the percentages of BrdU- cells with 2N
DNA content (G0/1), BrdU+ cells with 2N-4N DNA content (S), BrdU+ cells with
4N DNA content (G2/M) and apoptotic BrdU- cells with <2N DNA content (A).
Data are presented as mean ± SEM of 3 independent experiments. (C) TRAMP-
C2 cells were transduced with Mus81-specific shRNA (grey bars) or control
shRNA (white bars) and labelled with 10 μM CellTrace Violet. The labelled
13
shRNA-transduced TRAMP-C2 cells were then mixed with control shRNA-
transduced TRAMP-C2 cells labelled with 2 μM PKH-26 to obtain a 1:1 ratio. The
ratio of differentially labelled cells was analyzed prior to cell culture in vitro (0 h)
and 24 h after cell culture in vitro (24 h) by flow cytometry. (D) The differentially
labelled TRAMP-C2 cells described in (C) were mixed in a 1:1 ratio and injected
i.p. into C57BL/6 mice (n≥5). The ratio of differentially labelled TRAMP-C2 cells
was analyzed by flow cytometry prior to injection (0 h) and in the peritoneal
exudate 24 h after injection of the TRAMP-C2 cell mixture (24 h). *p<0.05.
Figure S6, related to Figure 6. Injection of TRAMP-C2 Cells Increases the
Percentage of Innate Immune Subsets in the Peritoneal Cavity
(A) Ifnb transcript levels in FACS-purified (>99% pure) CD11c+ (DC) and F4/80+
(Macrophage) cells were determined by real-time PCR 24 h after injection of
C57BL/6 mice with 6 × 106 with TRAMP-C2 (T) cells or PBS. Data represent
mean ± SD of n=5 mice (B) Graph representing fold change in lymphocyte
subset numbers in the mouse peritoneal exudates 24 h after i.p. injection of
TRAMP-C2 cells (grey symbols). Numbers were normalized to lymphocyte
subset counts in mice injected with PBS only (white symbols). Data represent
mean ± SEM of 3 independent experiments (n=5 mice each). (C-D) Graph
showing percentage of IFN-γ+ (C) or CD107a+ (D) lymphocytes in mouse
peritoneal exudates 24 h after injection with PBS only (white symbols) or
TRAMP-C2 cells (grey symbols). Data represent mean ± SD of n=5 mice. (E)
C57BL/6 mice were analyzed as described in (C) 48 hours after mice received
14
500 μg isotype control antibody (n=3; circles) or neutralizing anti-IFNAR antibody
(n=5; squares). (F) Natural killer (NK) cell depletion in C57BL/6 mice (n=5) was
confirmed by flow-cytometric analysis of blood CD3-CD49b+ cell numbers 24 h
after intraperitoneal administration of 500 μg isotype control antibodies (white
bar) or anti-NK1.1 (PK136) antibodies (grey bar). (G) Density plots on the left
show peritoneal exudate labelling for GR1 and F4/80 24 h after administration of
2 mg/ml clodronate liposomes (clodronate) or PBS-loaded control liposomes
(PBS). Boxes indicate the gating strategy used to identify GR1+ or F4/80+ cells.
Percentage of GR1+ (N) and F4/80+ (MP) cells in the peritoneal exudates are
indicated in the right panel. Data represents mean ± SEM of 3 independent
experiments (n=5 mice each). MP = macrophages; NKT = NK T cells. (H)
Macrophage depletion in C57BL/6 mice was confirmed by flow-cytometric
analysis of blood CD45+F4/80+CD11b+ cell numbers 60 h after intraperitoneal
administration of 600 μg isotype control antibodies (white bar) or anti-
CD115/CSF-1R (AFS98) antibodies (grey bar). Data represent mean ± SD of n=5
mice *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.
Figure S7, related to Figure 7. Proposed Model of Mus81-dependent Host
Rejection of Prostate Cancer Cells
The occurrence of MUS81 substrates in the genome promotes accumulation of
dsDNA in the cytosol of prostate cancer cells. Sensing of cytosolic dsDNA
triggers STING-mediated expression of type I IFNs and pro-phagocytic signals
15
leading to IFN-γ-dependent lysis of prostate cancer cells by macrophages and
potentially CD8+ T cells.
Table S1, related to Figure 3. Description of Human Cancer Samples Detailed patient and sample information is shown.
Figure S1
B DMSO Ara-CAphidicolin Hydroxyurea
dsDNA DAPI dsDNA
dsDNA DAPI dsDNA
dsDNA DPI dsDNA
dsDNA DAPI dsDNA
1 h 16 hA TRAMP-C2
1 h 16 hDU145
1 h 16 hPC-3
BrdU DAPI
DMSO Ara-C
0
50
100
150**
dsDNA DAPI dsDNA
dsDNA DAPI dsDNA
C
dsD
NA
MFI
/cel
l
DMSO Ara-C
Figure S2
D
0
10
20
30
40
Ctrl Mus81
dsD
NA
MFI
/cel
l
dsDNADAPI
Ctrl
Mus81
****C
GAPDH
Mus81
CtrlMus81CtrlMus81Mus81+/+Mus81-/-
E
Em
pty
vect
orpA
T 25t
etA
Mus81Mus81
Ctrl
GFPDAPI
Mus81
A
Fold
cha
nge
over
ctrl
Mus81#2Ctrl Mus81#10
1.0
0.5
Mus81#2shRNA
1.5
**** ****
Ctrl Mus81#10
1.0
0.5
Mus81#2siRNA
Ctrl Mus81#1siRNA
1.5
0
1.0
0.5
1.5
*** *** **** ***
TRAMP-C2 DU145 PC-3
dsD
NA
MFI
/cel
l
DMSOAph DMSOAph
****** ***
BMus81 shRNACtrl shRNA
0
810
dsDNADAPI
DMSO DMSO AphAph
46
2
Ctrl Mus81shRNA
AP
rost
ate
ca
nce
rN
L p
rost
ate
ca
nce
rB
rea
st
can
cer
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Bre
ast
ca
nce
rM
ela
no
ma
Ski
nC
olo
rect
al
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Co
lore
ctal
ca
nce
rIgG2aDAPI
IgG2aDAPI
B
En
do
me
tria
l ca
nce
rA
stro
cyto
ma
# n
ucl
ea
r MU
S8
1 fo
ci/c
ell
dsDNADAPI
MUS81DAPI
30
40
20
0
50
Ast
10
15
5
0AstEn
Cyt
o. d
sDN
A M
FI/c
ell
10
En
C
CL
LH
ea
lthy
dsDNADAPI
dsDNA
30
20
0CLLHealthy
Cyt
o. d
sDN
A M
FI/c
ell
10
**
Figure S3
D
0.1
1
10
100
0.1 1 10 100
Cyto dsDNA MFI/cell
r2=0.7323p<0.0001
# n
ucl
ea
r MU
S8
1fo
ci/c
ell
E
I II III
Stage
250
200
150
100
50
0
Cyt
od
sDN
AM
FI/c
ell
NL HP
********
***
*****
********
dsDNADAPI
dsDNA
NL
HP
I
II
III
STINGTubulin
StingCTRL StingCRISPR
1 2 1 2 3 4 5
Mus81-/- MEFsA
STINGTubulin
TRAMP-C2StingCTRL StingCRISPR
1 2 3
B
0
1000
2000
3000
IFN
-β e
xpre
ssio
n (p
g/m
l)
pDNAPoly I:C
StingCTRL StingCRISPR
+- +
- +- +
-
Mus81-/- MEFC
**** ****
0
200
800
1000
IFN
-β e
xpre
ssio
n (p
g/m
l)
600
400
TRAMP-C2D
Figure S4
--
--
pDNAPoly I:C
StingCTRL StingCRISPR
+- +
- +- +
---
--
**** ****
Figure S5
0
Rel
. cha
nge
in ra
tio
0 h 24 h
A
Ctrl Mus81shRNA
0
20
40
60
80
100
120
% c
ells
A <G0/1 S G2/M0
50
100
150B
% o
f cel
ls
1.5C
0.5
1.0
0
Rel
. cha
nge
in ra
tio0 h 24 h
3D
1
2
* *
Fold
cha
nge
of to
tal i
mm
une
cel
ls r
elat
ive
to m
ice
inje
cted
with
PB
SFo
ld c
hang
e ov
er P
BS
NK NKT γδT CD8α
% o
f IFN
-γ+
cells
NK NKT γδT CD8α
% o
f CD
107a
+ce
lls
NK NKT γδT CD8α
** ** **
B C D
0
20
40
60
80
0
10
20
30
40
0
10
20
30
40
MP
** * **
% o
f IFN
-γ+
cells
NK NKT γδT CD8α
E
0
10
20
30
40 ********
*** *******
% C
D3-
CD
49b+
cells
Isotype PK136
*
MP MP0
20
40
80
N NPBS
60
***
GR1
F4/80
ClodronateF PBS
% c
ells
in p
erito
neum
0
1
3
2
Clodronate
H
Isotype AFS98
*%
CD
45+ F
4/80
+ CD
11b+
cells
0
5
15
10
G
0
20
30
40
50
10
PBS PBST TDC Macrophage
Fold
cha
nge
over
PB
S
A** **
Figure S6
**
dsDNA
STING
Prostate Cancer Cell
CD8+ T cellsMacrophages/(Dendritic cells)
MUS81
Figure S7
Lysis?Phagocytosis
Type I IFNs
Type I IFNs
Type II IFN
Disease Area OriginCatalog Number Sample ID Case ID ASM Age Gender Sample Type Tissue of (Origin/Finding) Appearance
Sample Pathology from Pathology Verification
Case Diagnosis from Donor Institution Pathology Report Tumor Grade TNM
Minimum Stage
Grouping % Normal % Lesion % Tumor
% Tumor Hypercellular
Stroma
% Tumor Hypo/Acellular
Stroma%
Necrosis Pathology Verification Notes from H&E reviewProstate
Origene CS634963 FR0002C100 CU0000009443 BF6 42 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 pT2cpNXpMX II 0 90 10 0 0 0 Lesion: prostatic intraepithelial neoplasia; 30% glandular epithelium, 70% fibromuscular stromaOrigene CS634327 FR00028A54 CU0000009344 AF2 47 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 pT3apNXpMX III 0 95 5 0 0 0 lesion: hyperplasia of prostate; 60% epithelium, 40% fibromuscular stromaOrigene CS535985 FR0003992B CI0000014201 AF1 51 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 pT2bpN0pMX II 0 90 10 0 0 0 Lesion (90%): Hyperplasia of prostate 100%; Non Tumor Structures: 30% Epithelium, 70% Fibromuscular stromaOrigene CS523326 FR0002262B CI0000009014 BF5 75 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+2=5/10 pT1apNXpMX I 95 0 5 0 0 0 Non Tumor Structures: 30% Epithelium, 70% Fibromuscular stromaOrigene CS614627 FR00016CCF CU0000005026 Not reported 51 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 pT3apNXpMX III 0 95 5 0 0 0 Lesion: 100% hyperplasia of prostate containing 55% epithelium, 45% fibromuscular stromaOrigene CS530175 FR0003153A CI0000011643 Not reported 53 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 pT3apNXpMX III 0 90 10 0 0 0 45% epithelium, 55% fibromuscular stroma; lesion: 60% high grade prostatic intraepithelial neoplasia, 40% benign hyperplasia of prostateOrigene CS527465 FR0002DD13 CI0000010221 Not reported 62 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 pT2apNXpMX II 70 15 10 0 5 0 Lesion (15%): Prostatitis 100%; Non Tumor Structures: 25% Epithelium, 75% Fibromuscular stromaOrigene CS500048 FR0000178B CI0000000007 BF3 71 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 pT2cpNXpMX II 80 0 15 5 0 0 normal: 60% epithelium, 40% stromaOrigene CS502386 FR000063A4 CI0000000297 BF6 67 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 5+4=9/10 pT3bpNXpMX III 95 0 5 0 0 0 Not reportedOrigene CS500120 FR00001C2C CI0000000021 BF6 68 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Normal Within normal limits Adenocarcinoma of prostate n/a n/a n/a 100 0 0 0 0 0 30% Glandular epithelium, 70% StromaOrigene CS505199 FR00019502 CI0000005252 Not reported 48 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumour Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 pT2bpN0pMX II 0 90 10 0 0 0 70% epithelium, 30% fibromuscular stroma; lesion: benign prostatic hyperplasiaOrigene CS530165 FR00031450 CI0000011639 Not reported 50 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumour Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 pT3apN0pMX III 0 90 10 0 0 0 lesion: hyperplasia of prostate with prostatitis; 60% epithelium, 40% fibromuscular stromaOrigene CS503440 FR00007656 CI0000000421 Not reported 53 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Normal Within normal limits Adenocarcinoma of prostate Gleason Score: 4+3=7/10 pT2bpN0pMX II 100 0 0 0 0 0Origene CS514443 FR5B3391BF CI0000007160 Not reported 82 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Tumour Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 2+2=4/10 pT1apNXpMX I 70 0 10 10 10 0 Non Tumor Structures: 50% Epithelium, 50% Fibromuscular stromaOrigene CS505191 FR000188C5 CI0000005249 AF1 55 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Lesion Hyperplasia of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 pT2cpN0pMX II 0 100 0 0 0 0 60% epithelium, 40% fibromuscular stromaOrigene CS503715 FR00007DAD CI0000000470 FF1 51 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Lesion Hyperplasia of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 pT2bpN0pMX II 0 100 0 0 0 0 Non Tumor Structures: 55% Epithelium, 45% Fibromuscular stromaOrigene CS501085 FR000051A4 CI0000000128 AF3 60 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Lesion Hyperplasia of prostate Adenocarcinoma of prostate n/a n/a n/a 0 100 0 0 0 0 45% glandular epithelium, 55% stroma; benign prostate hyperplasiaOrigene CS634653 FR0002BCE4 CU0000009395 70 Male Frozen OCT-embedded Tissue Sections 5um Prostate / Prostate Lesion Hyperplasia of prostate Adenocarcinoma of prostate n/a n/a n/a 0 100 0 0 0 0 Non Tumor Structures: 40% Epithelium, 60% Fibromuscular stroma
SGH NA NA 07:PB25212 Not reported 76 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB12160 Not reported 80 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB15738 Not reported 54 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB24384 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB27762 Not reported 60 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05: PB01145 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB07213A Not reported 70 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB25270 Not reported 71 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+4=8/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB12434 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB15933 Not reported 60 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB27813 Not reported 55 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB07553 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB26984 Not reported 68 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+4=8/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB30743 Not reported 72 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB03421 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB02342 Not reported 64 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB07782 Not reported 58 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB30791 Not reported 49 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB16056 Not reported 74 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB21198 Not reported 71 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB02827 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB08314 Not reported 77 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB27516 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB30861 Not reported 42 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB4030 Not reported 57 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB21300 Not reported 57 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB25380 Not reported 64 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB28418 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB10537 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Tonsil / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB10537 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB27564 Not reported 67 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB04638 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB14417 Not reported 64 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB16378 Not reported 56 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB21817 Not reported 57 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB25441 Not reported 72 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB28923 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB03633 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+5=8/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB27755 Not reported 61 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB14485 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB23037 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB25646 Not reported 65 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB28979 Not reported 59 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 2+3=5/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB10579 Not reported 65 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB764 Not reported 61 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 5+3=8/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB05329 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB10068 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB15063 Not reported 46 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB17025 Not reported 57 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB23648 Not reported 61 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported II Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB30648 Not reported 53 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB04848 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB11407 Not reported 69 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB28091 Not reported 53 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB1434 Not reported 61 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+3=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB10683 Not reported 70 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB15114 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB17630 Not reported 74 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB24275 Not reported 55 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB00577 Not reported 67 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB05461 Not reported 71 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB11637 Not reported 65 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB28598 Not reported 53 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB1475 Not reported 59 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB05896 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB15661 Not reported 63 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB18257 Not reported 57 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB24321 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB06124 Not reported 61 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIC Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB10708 Not reported 67 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+3=6/10 Not reported IIIA Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB01054 Not reported 58 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 4+4=8/10 Not reported IIIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB04686 Not reported 66 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB9117 Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 06:PB03990 Not reported 56 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 05:PB01779 Not reported 60 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA 07:PB27444A Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Tumor Adenocarcinoma of prostate Adenocarcinoma of prostate Gleason Score: 3+4=7/10 Not reported IIIB Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH NA NA Not reported Not reported 62 Male Formalin Fixed Paraffin Embedded Tissue Prostate / Prostate Normal Within normal limits Adenocarcinoma of prostate n/a n/a n/a Not reported 0 0 0 0 0 30% Glandular epithelium, 70% Stroma
BreastOrigene CS517616 FR00025F07 CI0000007799 Not reported 44 Female Frozen OCT-embedded Tissue Sections 5um Breast/Breast Tumour Carcinoma in situ of breast, lobular Adenocarcinoma of breast, lobular Not reported pTXpN0pMX I 55 0 25 20 0 0 normal: 10% epithelium, 70% fibrotic stroma, 20% fatOrigene CS517615 FR00025F05 CI0000007799 Not reported 44 Female Frozen OCT-embedded Tissue Sections 5um Breast/Breast Normal Within normal limits Adenocarcinoma of breast, lobular Not reported pTXpN0pMX I 100 0 0 0 0 0 20% epithelium, 50% fibrotic stroma, 30% fat
SGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Breast/Breast Tumour Not reported Adenocarcinoma Not reported Not reported I Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Breast/Breast Tumour Not reported Adenocarcinoma Not reported Not reported I Not reported Not reported Not reported Not reported Not reported Not reported Not reported
MelanomaOrigene CS500090 FR00001A8D CI0000000017 Not reported 42 Female Frozen OCT-embedded Tissue Sections 5um Skin/Lymph Node Tumour Malignant melanoma, metastatic Malignant melanoma, metastatic Not reported pTXpN1pMX III 0 0 55 5 0 40 Not reportedOrigene CS558957 FR00043D4F CI7000000826 Not reported 49 Male Frozen OCT-embedded Tissue Sections 5um Lung Tumour Malignant melanoma, metastatic Malignant melanoma, metastatic Not reported pTXpNXpM1b IV 0 0 30 5 5 60 Not reportedOrigene CS520299 FR00026E16 CI0000008256 Not reported 49 Male Frozen OCT-embedded Tissue Sections 5um Lung Tumour Malignant melanoma, metastatic Malignant melanoma, metastatic Not reported pTXpNXpM1b IV 0 0 30 5 5 60 Not reportedOrigene CS610514 FR00006061 CU0000001603 Not reported 36 Female Frozen OCT-embedded Tissue Sections 5um Skin/Skin Normal Within normal limits No pathologic disease Not reported NA NA Not reported Not reported Not reported Not reported Not reported Not reported Not reported
ColorectalOrigene CS519420 FR00020B41 CI0000008078 Not reported 66 Male Frozen OCT-embedded Tissue Sections 5um Right colon/ Right colon Tumour Adenocarcinoma of colon Adenocarcinoma of colon Not reported pT3pN0pMX IIA 60 0 30 0 10 0 Not reportedOrigene CS535170 FR00038582 CI0000013783 Not reported 61 Male Frozen OCT-embedded Tissue Sections 5um Right colon/ Right colon Normal Within normal limits Adenocarcinoma of colon Not reported pT3pN0pMX IIA 100 0 0 0 0 0 Not reported
SGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Right colon/ Right colon Tumour Adenocarcinoma of colon Adenocarcinoma of colon Not reported Not reported II Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Right colon/ Right colon Tumour Adenocarcinoma of colon Adenocarcinoma of colon Not reported Not reported II Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Right colon/ Right colon Tumour Adenocarcinoma of colon Adenocarcinoma of colon Not reported Not reported II Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Right colon/ Right colon Tumour Adenocarcinoma of colon Adenocarcinoma of colon Not reported Not reported II Not reported Not reported Not reported Not reported Not reported Not reported Not reported
EndometriumSGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Not reported Tumour Endometrial Cancer Not reported Not reported Not reported 1B Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Formalin Fixed Paraffin Embedded Tissue Not reported Tumour Endometrial Cancer Not reported Not reported Not reported 1B Not reported Not reported Not reported Not reported Not reported Not reported Not reported
AstrocytomaOrigene CS511324 FR0001E199 CI0000006721 Not reported 34 Female Frozen OCT-embedded Tissue Sections 5um Brain/Brain Tumour Astrocytoma, anaplastic Astrocytoma, anaplastic WHO Grade III Not reported Not reported 0 70 10 0 10 10 lesion: 100% gliosisOrigene CS515704 FR00024763 CI0000007383 Not reported 39 Male Frozen OCT-embedded Tissue Sections 5um Brain/Brain Tumour Astrocytoma, anaplastic Astrocytoma, anaplastic WHO Grade III Not reported Not reported 0 0 60 0 20 20 Not reported
CLLSGH Not reported Not reported Not reported Not reported Not reported Not reported Frozen samples Bone marrow Tumour Not reported Not reported Not reported Not reported III Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Frozen samples Bone marrow Tumour Not reported Not reported Not reported Not reported III Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Frozen samples Bone marrow Normal Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reportedSGH Not reported Not reported Not reported Not reported Not reported Not reported Frozen samples Bone marrow Normal Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported Not reported