The Centre for Microscopy & Microanalysis NanoSIMS 50 NanoSIMS 50 nanoSIMS: a new analytical tool for ultra-fine feature analysis using secondary ion emission R. Stern* and Peta Clode The University of Western Australia Centre for Microscopy and Microanalysis [email protected]
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The Centre for Microscopy & Microanalysis NanoSIMS 50 nanoSIMS: a new analytical tool for ultra-fine feature analysis using secondary ion emission R.
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The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
nanoSIMS: a new analytical tool for ultra-fine feature analysis using secondary ion emission
R. Stern* and Peta ClodeThe University of Western AustraliaCentre for Microscopy and [email protected]
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
nanoSIMS labs
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The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
SIMS
Secondary Ion Mass Spectrometry probing analytical technique for measuring the surface
or near surface chemical or isotopic composition of solids
uses probe ions to sputter target ions in laterally microscale or smaller regions (ion microprobe)
analytical characteristics: nm depth resolution lateral resolution 10’s nm to m most sensitive of the probing analytical techniques, ppb-ppm entire periodic table, H to U simple sample preparation
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
SIMS Data
Dimensions: 1 dimension
‘depth profile’: composition along a line normal to the surface
‘line scan’: composition along a line parallel to the surface
2 dimensions ‘image’ (map) of the surface
distribution of secondary ion species 3 dimensions
a stacked series of secondary ion images collected at discrete depths into the target
Image
Depth profile
Line scan
ratios
18 16O/ O24Mg/28Si
Mass spectrum
cps,ppm Se
Basicfrom tiny mass consumed
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Sample under
vacuum
Ionsource
Primary ioncolumn
Secondary ionCollection
Optics
EnergyAnalyzer
Detector
MassAnalyzer
SIMS analytical
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Ion sources: O-, Cs+
Normal, co-axial primary & secondary
ions
Detector array: 5 isotopes acquired
simultaneously
High mass resolution analyzer
samples
Sample introduction
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Features of NanoSIMSdynamic SIMS
for bulk sample ion chemical/isotopic characterization not for molecular identification or oxidation state
probe lateral resolution 50 nm for 133Cs+
200 nm for 16O-
ability to scan the primary beam and record secondary ion images at ultra-fine-scale lateral resolution very low eroson rate, ~nm/hr (sample preservation)
simultaneous multicollection of up to 5 isotope speciesroutine high mass resolution
resolve isobaric interferences
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Ion imaging of TiCN alloy demonstrating sub-50 nm edge resolution with Cs+
10 x 10 um, 24C2
3 x 3 um, 26CN
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
44Ca / 40Ca 44Ca used as a tracer of calcium (40Ca) to determine pathways of movement & uptake
Region44Ca/40Ca
Natural abundance
0.02
1. Mucous cell 0.05
2. Spiked Seawater
0.7
3. Epithelial cells 0.37
4. Stinging cell 0.02
Significant
44Ca uptake
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Mouse cochlea cellsMouse cochlea cells
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Heavy metal exposure
Audinot et al. (2003)
Imaging of As within human Imaging of As within human hairhair
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
Analytical Issues:
will the metal ions be detectable? are there non-metallic ion labels that can be used as proxies for the metals? quantitation: how important? sample preparation to avoid element migration or loss
The Centre for Microscopy & MicroanalysisNanoSIMS 50NanoSIMS 50
nanoSIMS & ARC Metals in Medicine
is capable of playing a vital role the analytical capabilities and experience with metals in biological media needs to be developed R. Stern and P. Clode are here to enter into research partnerships with you