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The Antithrombotic and
Fibrinolytic Activity of theAqueous Extract from theCrude Latex of Ficus pumila
(Moraceae)Vien Christian L. Lansangan, Kathrizza T. Mabutas,
Rachelle Z. Ong, Arnold M. Perlas,Joencio !. A"ostol
University of anto TomasFaculty of !harmacy
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"ntroduction
Clotting# $%"ortant &e%ostasis %echanis% 'hich"reents e(cessie blood loss
# Platelet Aggregation)*Te%"orar+ Platelet Plug# Actiation o Coagulation Cascade)*-tableibrin crosslin
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"ntroduction
Clotting
# $s har%ul 'hen the blood clot/thro%busdislodges and blocs blood essels
0rain 1 CV Accident/ -troe Lungs 1 Pul%onar+ e%bolis%
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"ntroduction
Current Treat%ents2. Anticoagulants
3. 4ibrinol+tic Agents
5. Anti Platelet Agents
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#otanical $escri%tion
Kingdo% Plantae
6iision Magnilio"h+ta
Class Magnilio"sida Order 7rticales
4a%il+ Moraceae
!enus Ficus
-"ecies pumila
87- 6A9RC, 3:22;
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ynonyms
Cree"ing 4ig
Cli%bing 4ig4ig $+
82;
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Mor%holo&ical $escri%tionCree"ing 4ig
# "rostrate or cli%bing shrub.
Leaes# T'o)raned
# Oate, 2.> to 5 c% long
# Round or heart sha"ed base# ?ntire or 'a+ %argins
# On erect branches, > to 2: c%
Late(# ascular li@uid 'ith rubber)lie @ualities
4ruits# Large and bro'n colored
# Partiall+ "ear)sha"ed to B
c% o length 82;
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'bectives
2. To deter%ine the antithro%botic andibrinol+tic actiit+ o Ficus pumila.
3. To deter%ine i the antithro%boticactiit+ o Ficus pumila is co%"arable to
that o he"arin.5. To deter%ine i theibrinol+tic actiit+ o
Ficus pumila is co%"arable to that o-tre"toinase.
. To deter%ine the "ossible %echanis% b+'hich Ficus pumila "reents thro%bosis.
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Methodolo&y
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Methodolo&y !re%aration of F!AE
8Perello et al., 3:::;
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Ficus pumila fruit
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F!AE
!7M-
4PA?la+er
$9-OL70L?PART$C7LAT?-
• Turbid light)bro'n solution
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$etermination of CaseinolyticActivity of F!AE
8Perello et al., 3:::;
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"n vitro clot dissolution
8!ong et al., 3:22;
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"n *itroClot$issolution
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Acute Toxicity tudy
To deter%ine doses o 4PA? to bead%inistered.
0ased o O?C6 T! 3> guidelines
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Tail Thrombosis
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Methodolo&y tatistical Treatmentof $ata
All statistical anal+ses 'ere "eror%ed at:.:> leel o signiicance.
The Anal+sis o Variance 8A9OVA; 'asused to deter%ine 'hether signiicantdierences across treat%ents e(isted.
Tue+Ds %ulti"le co%"arisons test 'asutilized to identi+ signiicant dierences
bet'een s"eciic treat%ents.
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,esults and $iscussion
Late( Eield5: ruits 'eighing 2:F5.2g +ielded
23.:%L o late(The "ercentage +ield is 2.22G8/';
Caseinol+tic Actiit+4PA? e(hibited an aerage caseinol+tic
actiit+ o :.2==7cas/%L ro% 3 trials inthe "resence o 23%M o c+steineHCaseinol+tic 7nit87cas; is the a%ount o enz+%e that "roduces an increase o one absorbance unit"er rninute in the assa+ conditions
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,esults and $iscussion0lood Clot 6issolution
Fi&ure -. Mean !ercenta&e $issolution across treatment levelsA direct dose)clot dissolution relationshi" or 4PA? 'as obsered. At 2::) and2>:%g/5%L, the G blood clot dissolution 'as signiicant. -tre"toinase 2:$7 'asstatisticall+ "roen to be %ore eectie then 4PA? in the dissolution o blood clots
ater 3h "eriod. 2>:%g 4PA? 'as e@uialent to >3G o 2:,:::$7 o -tre"toinase.
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,esults and $iscussionPreention o Carrageenan)$nduced Tail Thro%bosis
&e"arin &e"arin 4PA? 4PA? 4PA? 9-- 2::$7 2:$7 23I%g/g F:%g/g >:%g/g
Fi&ure /. ?ects o 4PA? and &e"arin on %ice tail Thro%bus Lengths83 hours ater carrageenan ad%inistration;
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Fi&ure 0. Mean Len&th of Thrombosis (over 0+day %eriod) A direct dose)"reention o tail thro%bosis relationshi" or 4PA? 'as
obsered.
%
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,esults and $iscussion
Preention o Carrageenan)$nduced TailThro%bosisTest Samples Doses Inhibition of thrombosis (%)
24h 48h 72h
Heparin 100IU 94 92 92
10IU 87 80 80
FPAE 127mg/kg 60 61 60
80mg/kg - - 45
50mg/kg - - -
Fi&ure 0. Table of Mean Len&th of Thrombosis (over 0+day %eriod)4PA? at 23I%g/g 'as able to signiicantl+ "reent carrageenan)induced
tail thro%bosis oer the 5 da+ "eriod. 4PA? at F:%g/g had signiicant actiit+onl+ on the third da+.
%
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Conclusion4PA? "ossesses antithro%botic actiit+ in
io as it signiicantl+ "reentedcarrageenan)induced %ice tail thro%bosis.
The "ossible %echanis% o action is direct
ibrin h+drol+sis b+ icin as "roen in theblood clot dissolution test.# 4icin 'as "resent in 4PA? and had a caseinol+ticactiit+ o :.2==7cas/%L
4PA? at 23I%g/g is as eectie as 2:$7and 2::$7 Co%%erciall+ Aailable &e"arin.The ibrinol+tic actiit+ o 2>:%g 4PA? is
e@uialent to >3G o -tre"toinase 2::$7
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,ecommendations
To deter%ine other "ossible %echanis%so action# "latelet aggregation
# aPTT and PT
7se "uriied icin instead o crude enz+%ee(tract.
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,eferences
0ee%eier et al. 82=F>; Carrageenin)induced thro%bosis inrats and %ice a %odelor testing antithro%botic substances Agents Actions 2BB1>2.
!ong et al. 83:22;. Anti)thro%bosis eect o diosgenin e(tract ro% 6ioscoreazingiberensis C.&. right in itro and in io, Ph+to%edicine, Volu%e 2F, $ssue B.
&agi%ori et al. 83::=;. $%"roing re@uenc+ o thro%bosis b+ altering blood lo' inthe carrageenan)induced rat tail thro%bosis %odel. Phar%acological research theoicial ournal o the $talian Phar%acological -ociet+, B:8;, 53:)535.
Perello, M., Arribere, M. C., Caini, 9. O., N Priolo, 9. -. 83:::;. Proteol+tic ?nz+%esro% the Late( o 4icus "u%ila L. 8Moraceae;. Latin A%erican Journal o Phar%ac+ , 2=8;, 3>I)3B3.
2;. Medicinal Plants o the Phili""ines. Manila 6e"t. oAgriculture N 9atural Resources.
PLA9T- Proile or 4icus "u%ila. 83:22;. Retrieed 4ebruar+ 2B, 3:22, ro% 7-6e"art%ent o Agriculture 9atural Resource Conseration -ericehtt"//"lants.usda.go
/aa/"roiles+%bol4$P73
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