CAPTURE PURIFY ENRICH™ PhyNexus Thank you for joining the PhyNexus Webinar If you have questions, please type them with the Chat function. Access the Webinar audio with your computer speakers or submit your phone number and Webex will call you with the audio connection. The webinar will begin shortly. If you are experiencing problems, please call us at TEL: +1-408- 267-7214. Anne Burke Lee Hoang Jo Walter
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CAPTURE PURIFY ENRICH™ PhyNexus
Thank you for joining the PhyNexus Webinar
If you have questions, please type them
with the Chat function.
Access the Webinar audio with your
computer speakers or submit your phone
number and Webex will call you with the
audio connection.
The webinar will begin shortly.
If you are experiencing problems, please call us at TEL: +1-408-
267-7214.
Anne Burke
Lee Hoang
Jo Walter
CAPTURE PURIFY ENRICH™ PhyNexus
Lee Hoang, Ph.D.
R&D Manager
PhyNexus, Inc.
San Jose, CA
Innovative Solutions for Automated
Protein and Nucleic Acid Purification
CAPTURE PURIFY ENRICH™ PhyNexus
Current small-scale sample preparation
have drawbacks
CAPTURE PURIFY ENRICH™ PhyNexus
PhyTip® column design
Top screen
Bottom screen
Resin maintained in discrete area
CAPTURE PURIFY ENRICH™ PhyNexus
PhyTip® column design
5 20 10 20 40 80 160 320 L 200 L 600 L
Traditional PhyTip Columns Gel Filtration PhyTip Columns
CAPTURE PURIFY ENRICH™ PhyNexus
PhyTip Columns
4x
Capture
Pro Pro
Pro Pro
Pro Pro
Pro
Pro
Pro
Target Protein
Contaminants
Buffer
Pro
CAPTURE PURIFY ENRICH™ PhyNexus
PhyTip Columns
4x
Purify
Pro
Pro Pro Pro
Pro
Pro Pro
Pro
Pro
Target Protein
Contaminants
Wash Buffer
Pro
CAPTURE PURIFY ENRICH™ PhyNexus
PhyTip Columns
4x
Enrich
Pro
Pro Pro
Pro
Pro Pro Pro
Pro
Pro
Target Protein
Elution Buffer
Pro
CAPTURE PURIFY ENRICH™ PhyNexus
Reciprocal flow, small resin bed size, and low dead volume
allow for efficient processing of small samples
CAPTURE PURIFY ENRICH™ PhyNexus
Beckman FX
Caliper Sciclone & Zephyr
Perkin Elmer Minitrak
Tecan Freedom Evo Dynamic Devices Oasis
Hamilton STAR
PhyTip Columns – Compatible with Popular Liquid Handling Robots ®
Velocity 11 Bravo
CAPTURE PURIFY ENRICH™ PhyNexus
MEA for fully automated, medium throughput sample processing