Th1_Gene Pyramiding to Improve Green Super Rice by Molecular Marker-assisted Selection

Gene Pyramiding to Improve Green Super Rice by Molecular Marker-assisted Selection

Yuqing He

National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural UniversityWuhan, ChinaEmail: [email protected]

The Problems of hybrid rice in China

• Lose of disease resistance (blast, bacterial blight)

• Susceptible to insect pest (yellow stem borer and brown planthopper)

• Cooking and eating quality• Ever increasing demands for increase yield

Strategies for developing Green Super Rice

Green Super Rice should possess resistances to

multiple insects and diseases, high nutrient

efficiency, and drought resistance, promising

to greatly reduce the consumption of

pesticides, chemical fertilizers, and water.

Zhang, PNAS, 2007, 16, 104 (42): 16402–16409

Bt gene to improve hybrid rice with stem borer and leaffolder resistance

Tu, J.et al. Nature Biotech, 2000,18:1101-1104

Bacterial Blight resistance to improve hybrid rice

G14652.0

RG11091.5

C50

2.8

C1331.0C10030.8C1890.8RG103212483.0

AB9

3.0

CDO534

Xa21

Chr. 11

Xa7

M5

4.0

STSP3

0.9

AFLP1415

Marker from IRRI

Chr. 6

Chen et al. Crop Science, 2000, 40: 239-244

Zhang et al， Plant Breed， 2006， 125, 600-605

Xa23

Genotype Genes No. GX325 Mean KS-1-21 Mean

xa21xa21xa7xa7 0 A 19.58 A 22.071

xa21xa21Xa7xa7 1 B 12.38 B 4.20

Xa21xa21xa7xa7 1 C 6.47 C 7.96

Xa21Xa21xa7xa7 2 D 4.84 D 5.05

xa21xa21Xa7Xa7 2 D 4.31 C 7.16

Xa21Xa21Xa7xa7 3 E 2.67 D E 3.64

Xa21xa21Xa7xa7 2 E 2.31 E 3.33

Xa21xa21Xa7Xa7 3 E 2.10 E 3.09

Xa21Xa21Xa7Xa7 4 E 1.61 E 2.23

Comparison of bacterial resistance to GX325 and KS-1-21 as shown by lesion length

among nine genotypes of an F2 population using Tukey-Kramer HSD test

MH63(Xa21) and MH63(CK)

The locations of Pi-1 and Pi-2

Y2668LAY6854L

R251

RM224

R543a

RZ536

4.5

5.1

2.2

6.8

9.7

5.1

BC1F2 of

Zhenshan 97/BL5

Chr 11

Pi-1

C235

RG64

AP22

RG445

RZ632(R2123)

1.2

0.3

1.5

0.4

Pi-20.9

Chr 6

F5 (CO39/C101A51) RILs

Pi9, Pigm, R6

NILs gene Infection strains

Resistance frequency (%)

C101LAC Pi1 13 82.7C101A51 Pi2 11 85.3C104PKT Pi3 57 24.0

BL1 Pi1+Pi3 8 89.3BL2 Pi1+Pi3 7 90.7BL5 Pi1+Pi3 5 93.3BL3 Pi2+Pi3 4 94.7BL4 Pi2+Pi3 5 93.3BL6 Pi1+Pi2+Pi3 2 97.3

CO39 （ ck） no 75 0

Zhenshan97 unknown 46 38.7

Reaction of Pyricularia grisea isolates on the differential near isogenic lines (NILs) (Liu et al, 2003)

Blast natural identify area

Yuan’an, Hubei (Altitude:550m)

Enshi, Hubei

（ Altitude:600m ）

Fig. 2 The distribution of blast

resistance scores of the improved

versions of Jin 23B and their hybrids

compared with control varieties. a the

leaf blast resistance scores of the

improved Jin 23B, hybrids, and control

at tillering stage. b the leaf blast

resistance scores of the improved Jin

23B, hybrids and control at heading

stage. c the neck blast resistance scores

of the improved Jin 23B, hybrids and

control at maturity stage.

Jiang et al, Mol Breed: 2012

Bph14

QBph3

Bph11(t)RM514

SG1

15.0

SG6

9.0

RM570

8.0

IN762

10.0

J14-87.0

J14-127.0

Chr 3RM8213

RM164342.2

RM261

19.1

HJ163.6

IN1560.7

J23/MS52.2

HJ28

10.1

RM5953

11.5

Bph12

Bph17

Bph15

QBph4.1

QBph4.2

Bph20

Chr 4aRM307

HJ22

20.6

RM167172.9

RM16820

29.3

RM168536.1

J6-4

21.2

RM119

1.0

J6-100.9

RM252

26.9

bph16

Bph27

Bph6

Bph24

Chr 4b

RM313

RM463

12.5

J18-15

5.2

J18-4

0.2

IN187

2.1

HJ12

2

RM3331

4.2

HJ9

4

RM28625

6.6

RM2704.7

bph2

Bph10

Bph18

Bph9

Bph21

Bph1

Chr 12

BPH resistance genes

Hu et al, Mol Breed (2012) 29:61–69Hu et al, PMS, Pest Manag Sci (2013) 69: 802–808

0

1

2

3

4

5

6

7

8

9

6303

S/93

11(1

4/15

/18)

6303

S/93

11(1

4/15

)

6303

S/93

11(1

4/18

)

6303

S/93

11(1

5/18

)

6303

S/93

11(1

4)

6303

S/93

11(1

5)

6303

S/93

11(1

8)

6303

S/93

11

GZ

63S/

9311

(14/

15/1

8)

GZ

63S/

9311

(14/

15)

GZ

63S/

9311

(14/

18)

GZ

63S/

9311

(15/

18)

GZ

63S/

9311

(14)

GZ

63S/

9311

(15)

GZ

63S/

9311

(18)

GZ

63S/

9311

Y58

S/93

11(1

4/15

/18)

Y58

S/93

11(1

4/15

)

Y58

S/93

11(1

4/18

)

Y58

S/93

11(1

5/18

)

Y58

S/93

11(1

4)

Y58

S/93

11(1

5)

Y58

S/93

11(1

8)

Y58

S/93

11

B5(

14/1

5)

Hu7

(18)

TN

1

Res

ista

nce

Scor

e

Hybrids

The resistance score of pyramided two-line hybrids

21 days

BPH resistance in field experiment (4 hectares)(2010, Tongcheng, Hubei)

GZ63S/9311(ck) GZ63S/9311(Bph14/Bph15)

GZ63S/9311(ck) GZ63S/9311(Bph14/Bph15)

Whole genome selection breeding

R6: blast resistance gene60K SNPs Background selection

Kongyu 131(Pi2)

Pi2

Ph D and postgraduate: Hu JieYuan LiGao Guanjun Tu JumingChen Sheng Jiang Gonghao Zhang Jinfeng Yang Zixian Li Xin Liu Shiping Wu Changjun

Acknowledgement

StaffRice group

Collaboration:

IRRI

SAGC

CAAS

Wuhan University

Cornell University

Supported: 863 plan; National Natural Science Foundation

in China and Bill and Melinda Gates Foundation

Thank You!