Testing of beverages a testing lab’s perspective

Testing of beverages a testing lab’s perspective

Challenges and opportunities in testing beverages

What all is to be tested?

Chemical Testing Quality parameters

Safety Parameters

Nutritional Parameters

Biological Testing Indicator and Hygiene Parameters

Pathogens

Viruses and parasites

GMO detection

Meat and Fish Species identification

What to test and methods to follow?

Standard products – easy – do what is in the act and rules

Proprietary/Non standard – not in the Act and Rules – risk based approach

Methods – FSSAI manual?

Standard methods?

In-house validated methods?

Must verify!!!!!!

Must test multiple batches in the beginning!!!!!!

Capabilities requiredMajor tests that need to be conducted by the laboratory

Shelf life estimation Real-time vs Accelerated

What protocol to follow?

Test? Quality? Assay for claim ingredients? Sensory? Safety? Microbiology? Pack integrity? Acceptance criteria? Methods – verified or valiated for

the product

Time points? Real-time?

Accelerated

What multiplier?

WVTR and OTR enough?

What storage temperature? 37, 40, 45, -20, 2 C

Humidity? 75%, 90%, 30% or uncontrolled

Nutritional analysis

Proximate analysis

Capabilities Fat

Protein

Carbohydrate

Energy

Ash

Moisture

Equipment Automated soxhlet

CHNS & Auto Kjeldahl

Bomb calorie meter

Thermo gravimetric analyser

RI HPLC?

GC FID?

Sugar profile

Capabilities Glucose

Fructose

Sucrose

Trehalose

Triose, Tetrose etc

Equipment HPLC – RI

Amino acid profile

Capabilities Protein powders

Nutraceuticals

Free amino acids

Amino acid profile of protein

Equipment HPLC – FLD

HPTLC

Fat and water soluble Vitamins

Capabilities Juices

Fortified foods

Nutraceuticals

All vitamins Water soluble – Vitamin C, B1, B2,

B6, B12

Fat soluble – Vitamin A, E, D, K

Equipment HPLC

LCMSMS

Essential and trace element

Capabilities Analysis of elements from 100% to

ppb levels

Methods for various matrices Fortified foods

Packages foods

Nutraceuticals

RTE etc

Equipment ICPMS

Ion chromatography

Auto titrators

Microwave digestion

Fibre analysis

Capabilities Crude fibre

Dietary fibre

Equipment Automated fibre analyser

HPLC RI Detctor

Herbal ingredients

Capabilities Chromatographic fingerprinting

Marker compound quantification

Poly herbal formulations

Equipment HPTLC

HPLC

GC

GCMSMS

LCMSMS

Fatty acid profile

Capabilities Fats and oils

Nutraceuticals

Packaged foods etc

All fatty acids

Trans fats

Cholesterol

Equipment GC – FID

FTIR

Claims verification

Claims Sugar free High protein Isotonic Low fat Low salt Trans fate free High fibre content Cholesterol free Preservative free Organic

Equipment Automated soxhlet Auto titrator GC Fibertech RI HPLC GC HPLC/LCMSMS LCMSMS, GCMSMS CHNS – dumas method

Food Safety Parameters Comprehensive menu of tests covering all tests under FSSA and more

Pesticide Residue

Capabilities Capability to analyse all

pesticides in FSSA - 149

Detection levels below than current regulations

Various matrices

Equipment GCMSMS

LCMSMS

HPLC

Sample mills

Homogenizers

Sample concentrator and hoods

Heavy metals

Capabilities Ability to analyse all toxic metals

at ultra trace levels

Using standard international methods

Methods suitable for variety of food matrices

Equipment ICPMS

Microwave digestion system

Fume hoods

Clean lab

Ultra pure acids

NOTS – Naturally occurring toxins

Capabilities All NOTS as per FSSA

Safrole

Hypercine

Hydrocyanic acid

Mycotoxins

Equipment HPLC – FLD

HPTLC

GCMSMS

LCMSMS

Ion chromatography

Mycotoxins

Mycotoxins Aflatoxins – B1, B2, G1, G2

Ochratoxin

Patulin

ZON, DON

Equipment HPLC – FLD

LCMSMS

ELISA – for screening

Vet drug residues

Capabilities All drugs and matrices as per FSSA

Nitro furans

Tetracycline

Sulphonamides

Methods validated to global standards

Various matrices – Honey, Fish, Milk, Egg

Deuterated internal standards

Equipment LCMSMS

HPLC

ELISA – for screening

Solid phase extraction (SPE)

Mills

Homogenizers

Nitrogen evaporators

Microbiology

Capabilities Pathogen detection

Hygiene monitoring Swabs

Air sampling

Probiotics

Vitamin assays

Facilities Clean rooms

LAF

Biosafety cabinet

Incubators

Freezers

Autoclaves

Food Additives

Capabilities ID and quality of additives

ID and level of additives in food

Food colour – permitted & levels

Preservatives

Anti oxidants

Thickeners, Modifiers, emulsifiers & more

Equipment HPLC

GC

FTIR

HPTLC

GCMSMS

Ion chromatography

Packing material testing?New regulation

Thank you! E-mail – [email protected]

State of the Art equipment

LCMSMS with UPLC Front end

ICP - MS

HPTLC – for Herbal Products

GC-HS-FID and GC-ECD