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Introduction to respiration
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Teaching notes
This resource is the perfect introduction to respiration in which students discover
aerobic and anaerobic respiration for themselves through a series of straightforward
activities. This activity is aimed at high ability students. The activities are arranged as
a circus of eight stations around the room. There is a worksheet which students should
fill in as they visit each station. The answers could be provided at each station in an
envelope or back to back with the activity sheet in a plastic wallet.
Equipment required
Station 2 - beaker of respiring yeast
Station 3 - models of generalised animal and plant cells
Station 6 – The experiment needs to be set up as a dummy so that students can predict
the results. 4 test-tubes set up as below, but with pink coloured water in place of the
indicator solution.
Station 7 - flasks with boiled and germinating peas set up as diagram below. Or
something to suggest a similar experiment e.g. two bunged opaque flasks, with
thermometers one containing hot water, and the other containing room temp water.
Station 8 - test-tubes of limewater with straws, one for each student
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Introduction to respiration
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Respiration in cells can take place aerobically or anaerobically. The energy released is used in a variety of ways. Visit the stations around the room to complete this worksheet.
Equation for aerobic respiration
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Equation for anaerobic respiration in animals (used to obtain energy if insufficient oxygen is reaching the muscles for aerobic respiration)
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Equation for anaerobic respiration in organisms such as yeast
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Station 1: Which types of athletic events require anaerobic respiration? What is the
downside?
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Station 2: Why will anaerobic respiration eventually kill yeast?
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What do we use the products of respiration in anaerobic yeast for?
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Can you name other food products that utilise anaerobic respiration?
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Introduction to respiration
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Station 3:
Name the structures used in respiration in animal and plant cells. ……………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………..
Station 4:
To prevent lactic acid build up, endurance athletes limit their heart rate to prevent anaerobic respiration. To stay below the lactic acid threshold (the point where blood lactic acid concentration starts to rise) Jude’s maximum heart rate
needs to be: …………………………………………………………………………………………………………………………………………………………………………………………………………………………………..
Station 5:
During an aerobics class, what would happen to your body temperature and breathing rate? Why?
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Aerobic respiration is a series of reactions which can summarised by this equation.
C6H12O6 + 6O2 → 6CO2 + 6H2O (+ energy released) glucose oxygen carbon dioxide water
Does the above equation look familiar – which other process does it remind you of?
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Introduction to respiration
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Station 6: Hydrogencarbonate indicator is pink. It turns yellow if CO2 is added and
purple if CO2 is removed.
Colour the indicator in each tube to show the results you would expect to see after 1 hour.
Why does the 3rd tube have foil around it?
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What is the purpose of the 4th tube?
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Station 7: Which results are for which flask? How do you know?
Time (hours)
Temperature (°C)
Flask_____ Flask_____
0 15 15
12 15 20
24 15 25
36 15 29
48 15 31
60 15 33
72 15 33
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Introduction to respiration
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Station 8: Limewater turns cloudy in the presence of CO2
When I breathed into limewater it turned .........................................
this proves that my exhaled air contains ................................... so I am respiring.
Extension tasks:
As the breakdown of glucose is incomplete in anaerobic respiration, much less energy is
released than during aerobic respiration. Research the difference in energy released per
gram of glucose used.
Anaerobic respiration results in an oxygen debt – what does this mean?
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Introduction to respiration
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Station 1 – information sheet
Anaerobic respiration
glucose → lactic acid (+energy released)
incomplete breakdown of glucose
Discuss the following questions in your pairs and then fill in your answers on your sheet.
Which types of athletic events require anaerobic respiration?
What is the downside to this form of respiration?
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Introduction to respiration
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Station 1 – answer sheet
Anaerobic respiration occurs during high-intensity activities e.g. sprinting
If muscles are subjected to long periods of vigorous activity
they become fatigued, i.e. they stop contracting
efficiently. One cause of muscle fatigue is the build-up of
lactic acid in the muscles which causes cramps. Blood
flowing through the muscles removes the lactic acid.
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Station 2 – information sheet
1
Discuss the following questions in your pairs and then fill in your answers on your sheet.
This will eventually kill the yeast? Why?
What do we use the products of this type of respiration for?
Can you name other food products that utilise anaerobic respiration?
1 Yeast cells/ Credit: SciMAT / Photo Researchers / Universal Images Group/ Copyright © © Photo Researchers / For Education Use Only. This and millions of other educational images are available through Britannica Image Quest. For a free trial, please visit www.britannica.co.uk/trial
Yeast cells respire anaerobically producing ethanol and carbon dioxide:
glucose → ethanol + carbon dioxide (+energy released)
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Introduction to respiration
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Station 2 – answer sheet
glucose → ethanol + CO2 (+ energy released)
Yeast cells respire anaerobically – eventually the ethanol build-up will become toxic to the yeast.
Ethanol is used to make alcoholic drinks
CO2 is used to make bread rise
All of these food products utilise anaerobic respiration – yoghurt, cheese, vinegar
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Station 3 – information sheet
Name the structures used in respiration in plant and animal cells.
Station 3 – answer sheet
Most of the reactions in aerobic respiration take place inside mitochondria.
Where enzymes are made. Location of reactions in anaerobic respiration
Holds genetic code for enzymes involved in respiration
Contains the enzymes for aerobic respiration.
Allows gases and water to pass freely into and out of the cell. Controls the passage of other molecules.
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Station 4 – information sheet
Anaerobic respiration is essential for short bursts of energy but cannot be used for
longer periods. Endurance athletes need to pace themselves to avoid anaerobic
respiration. A build-up of lactic acid would hinder their performance.
Jude should keep her heart rate just below the lactic acid threshold (the point
where her blood lactic acid conc. starts to rise) when she trains.
What should her maximum heart rate be?
Station 4 – answer sheet
Maximum heart rate should be somewhere between 155 and 165 beats per minute.
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Introduction to respiration
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Station 5
During an aerobics class, what would happen to your body temperature, heart rate
and rate and depth of breathing? Why?
Aerobic respiration is a series of reactions which can summarised by this equation.
C6H12O6 + 6O2 → 6CO2 + 6H2O (+ energy released) glucose oxygen carbon dioxide water
Does the above equation look familiar?
Station 5 – answer sheet
During an aerobics class, what would happen to your body temperature, heart rate
and rate and depth of breathing? Why?
They would all increase. These changes increase the blood flow to the muscles and so
increase the supply of sugar and oxygen and increase the rate of removal of carbon
dioxide. Heat is a by-product of respiration.
C6H12O6 + 6O2 → 6CO2 + 6H2O (+ energy released) glucose oxygen carbon dioxide water
The equation for aerobic respiration (above) is the reverse of the equation for
photosynthesis (below).
6CO2 + 6H2O light energy
C6H12O6 + 6O2
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Station 6
Hydrogencarbonate indicator is pink. It turns yellow if CO2 is added and purple if CO2 is
removed.
Discuss the following questions in your pairs and then fill in your answers on your sheet.
Predict the colour of the 4 tubes.
Why does the 3rd tube have foil around it?
What is the purpose of the 4th tube?
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Station 6 – answers
Predict the colour of the 4 tubes – see below
Why does the pondweed tube have foil around it?
This prevents the pondweed from photosynthesising. Depending upon the rate of
photosynthesis vs. respiration the indicator could be pink, yellow or purple!
What is the purpose of the 4th tube?
Control
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Station 7
Susan set up the apparatus as shown. Both sets of peas were sterilised to kill microbes
on their surfaces. She measured the temperature inside each flask every 12 hours. Her
results are shown in the table below, but she forgot to label her table.
Which results are for which flask? How do you know?
Time (hours)
Temperature (°C)
Flask? Flask?
0 15 15
12 15 20
24 15 25
36 15 29
48 15 31
60 15 33
72 15 33
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Station 7 – answer sheet
Time (hours)
Temperature (°C)
Flask B Boiled peas
Flask A Peas soaked in water
0 15 15
12 15 20
24 15 25
36 15 29
48 15 31
60 15 33
72 15 33
The peas are in flask A respiring and respiration produces heat. The peas in flask B were dead (had been boiled) so could not respire and release heat energy.
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Station 8
The test-tubes contain limewater which turns cloudy in the presence of CO2
Breathe out gently into the straw.
Health and safety - DO NOT SUCK UP THROUGH THE STRAW.
You may need breathe out several times to cause a change.
What happens?
Does this show you are respiring aerobically or an anaerobically?
Health and safety - Remove the straw when you have finished to show the test tube
has been used.
Station 8 – answers
When you breathed into the limewater it should have turned cloudy – a positive
test for carbon dioxide.
The presence of CO2 in your exhaled air proves you are respiring aerobically.
C6H12O6 + 6O2 → 6CO2 + 6H2O (+ energy released) glucose oxygen carbon dioxide water
Anaerobic respiration in animals – no carbon dioxide is produced.
glucose → lactic acid (+ energy released)