Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis Tai LT, Daran-Lapujade P, Walsh MC, Pronk JT, Daran JM (2007) American Society for Cell Biology 18: 5100-5112 Alex George Bobek Seddighzadeh Journal Club Presentation BIOL 398-01/S10: Bioinformatics Lab April 13, 2010
19
Embed
Tai LT, Daran-Lapujade P, Walsh MC, Pronk JT, Daran JM
Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis. Tai LT, Daran-Lapujade P, Walsh MC, Pronk JT, Daran JM. (2007) American Society for Cell Biology 18: 5100-5112. Alex George Bobek Seddighzadeh Journal Club Presentation - PowerPoint PPT Presentation
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based
Transcriptome Analysis
Tai LT, Daran-Lapujade P, Walsh MC, Pronk JT, Daran JM
(2007) American Society for Cell Biology 18: 5100-5112
Alex GeorgeBobek Seddighzadeh
Journal Club PresentationBIOL 398-01/S10: Bioinformatics Lab
April 13, 2010
Outline
• DNA microarrays determine gene expression• Chemostat cultures foster constant growth rates• Suboptimal temperatures influence cellular processes• Investigate steady state acclimatized growth of
suboptimal temperature growth of S. cerevisiae.• In-depth analysis of the tables and result presented
in the study • Discussion on temperature and growth rate changes
that illicit a transcriptional response
DNA microarrays are used to determine gene expression levels
• Each spot contains a probe that correlates to a gene
• Labeled sample is washed over chip to form complementary bonds
• Strength of fluorescent signal indicates more binding
• Our experiment: Only one sample hybridized at a time- relative abundance
Chemostat cultures maintain a constant specific growth rate
• Batch culture- limited supply of nutrients provided
• Chemostat culture- nutrients are continuously provided in order to control specific growth rate
• Specific growth rate has been shown to impact transcript profiles
Suboptimal Temperatures Affect Various Cellular
Processes/Characteristics• Optimum growth range for S Cerevisiae: