Course: Systems Neuroscience 1. Func4onal neuroanatomy d. Func4onal imaging techniques II (twophoton imaging) Shin Nagayama (MSE R438) Goal of this lecture Understand the basic principle of twophoton imaging technique. Understand how the twophoton imaging system has been contributed to Neuroscience. Understand the fronDer of twophoton opDcal imaging technique.
21
Embed
Systems Neuroscience Course 2015 - Functional Neuroanatomy · 2019. 4. 24. · Systems Neuroscience Course 2015 - Functional Neuroanatomy Author: Shin Nagayama, Ph.D. Created Date:
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Course: Systems Neuroscience 1. Func4onal neuroanatomy d. Func4onal imaging techniques II (two-‐photon imaging) Shin Nagayama (MSE R438)
Goal of this lecture Understand the basic principle of two-‐photon imaging technique.
Understand how the two-‐photon imaging system has been contributed to Neuroscience.
Understand the fronDer of two-‐photon opDcal imaging technique.
1. Principle of two-‐photon microscopy
Principle Merit/Demerit
2. Applica4on (in vivo) Single cell imaging (DendriDc integraDon)
PopulaDon imaging (Different cell types) Neurons associated with same network module
3. Fron4er of New techniques Increase the speed of imaging: AOD scanning 4D imaging
Two-‐Photon microscope Two-‐photon excitaDon 1. Principle of two-‐photon microscopy
Principle
E=hc/λ
Energy of photon (E) and Wave length of the light (λ) are given by the equaDon
hc=1.99 x 10-‐25 h is Planck’s constant c is the speed of light
Two-‐Photon microscope Two-‐photon can excite probe just in the focus area
1. Principle of two-‐photon microscopy Principle
Single-‐Pho
ton
Two-‐Ph
oton
Two-‐Photon microscope
Two-‐photon excitaDon breaches fluorescence just in the focus plane
1. Principle of two-‐photon microscopy Principle
Two-‐Photon microscope Two-‐photon imaging system efficiently collect emission light
1. Principle of two-‐photon microscopy Principle
• Merit: deep layer imaging, lower photo damage, efficient photon collecDon – >> fit to in vivo funcDonal imaging