1 Synthesis of azoxystrobin transformation products and selection 1 of monoclonal antibodies for immunoassay development 2 Javier Parra a,§ , Josep V. Mercader a,§ , Consuelo Agulló b , 3 Antonio Abad-Somovilla b, *, and Antonio Abad-Fuentes a, * 4 5 a Department of Biotechnology, Institute of Agrochemistry and Food Technology, IATA−CSIC, Agustí 6 Escardino 7, 46980 Paterna, València, Spain 7 b Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, 8 València, Spain 9 10 11 12 § Both authors contributed equally to this work. 13 14 15 * Corresponding authors: Dr. A. Abad-Fuentes, Tel. +34-963900022; Fax +34-963636301; e-mail 16 [email protected]and Dr. A. Abad-Somovilla, Tel. +34-963544509; Fax +34-963544328; e-mail 17 [email protected]18 19 *Manuscript Click here to view linked References
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Synthesis of azoxystrobin transformation products and selection 1
of monoclonal antibodies for immunoassay development 2
Javier Parraa,§, Josep V. Mercadera,§, Consuelo Agullób, 3
Antonio Abad-Somovillab,*, and Antonio Abad-Fuentesa,* 4
5
a Department of Biotechnology, Institute of Agrochemistry and Food Technology, IATA−CSIC, Agustí 6
Escardino 7, 46980 Paterna, València, Spain 7
b Department of Organic Chemistry, Universitat de València, Doctor Moliner 50, 46100 Burjassot, 8
València, Spain 9
10
11
12
§ Both authors contributed equally to this work. 13
14
15
* Corresponding authors: Dr. A. Abad-Fuentes, Tel. +34-963900022; Fax +34-963636301; e-mail 16
[email protected] and Dr. A. Abad-Somovilla, Tel. +34-963544509; Fax +34-963544328; e-mail 17
AZo6#49 1.01 0.02 1.69 <0.01 <0.01 a Values correspond to cross-reactivities referred to the IC50 (nM) of azoxystrobin, which is considered 100%. Assays were carried out in the
homologous indirect cELISA format, using for every combination of mAb and conjugate those concentrations resulting in the lowest IC50 value for
azoxystrobin with an Amax higher than 1.0.
N N
O O
MeO2CCN
OMe
N N
O O
OMeHO2C
CN
N N
O O
MeO2CCN
OH
N N
O O
CO2H CN
N N
HO O
CN
Table 2
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Toxicology LettersConflict of Interest Policy
' r : . , i r r i l l :
Synthes¡s of azorystrob¡n transformation products andselection of monoclonal antibod¡es for ¡mmunoassaydevelopment
Declarat¡onsToxicology Leúters requires that all authors sign a declarat¡on of conf¡cting interests. ¡f you havenothing to declare ¡n any of these categor¡es then this should be stated.
Conflict of Interest and Source of Fund¡ngA conflict of interest ex¡sts when an author or the author's institution has a financial or other relationshiow¡th other people or organizat¡ons that may inappropr¡ately ¡nfluence the author,s actions. Allsubmissions to Tox¡cology Leffers must ¡nclude disclosure of all relationships that could be viewed aspresent¡ng a potential confl¡ct of inleresl. Tox¡cology Leffers may use such information as a bas¡s foreditor¡al decisions and may publ¡sh such disclosures ¡f they are bel¡eved to be ¡mportant to readers injudg¡ng the art¡cle.
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Please state any sources of funding for your research
This work was supported by M¡n¡sterio de Educdc¡ón y C¡enc¡d (AGL2OO6-LZ7SO-CO2-OL/02/ALtland cofinanced by FEDER funds. J.P. and J.V.M. were h¡red bV the CSIC, the former under apredoctoral /3P contract and the latter undet a Ramón y Cajal postdoctoral contract, both of themfinanced by the Spa nish M¡nister¡o de Cienc¡o e lnnovoción and the EuroDean Social Fund.All above mentioned funding bodies had no role in the des¡gn, the contents, or the writ ing of thispaper, nor ¡n the dec¡sion to subm¡t th¡s paper for publication.
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Antonio Abad-Fuentes
Antonio Abad-Fuente s
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*Conflict of Interest Statement
Toxicology LettersConflict of Interest Pol¡cy
. . : t . , ¡ : t r i . , :Synthesis of azorystrob¡n transformat¡on products andselection of monoclonal antibodies for lmmunoassaydevelopment
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DeclarationsToxicotogy Letters requires that all authors sign a declarat¡on of confl¡ct¡ng interests. lf you havenothing to declare ¡n any ofthese categories then this should be stated.
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Javier Parra
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Role of Funding SourceAll sources of funding should be declared in the box below. Authors must also descr¡be the role of thestudy sponso(s), ¡f any, ¡n a study des¡gn; in the coltection, analysis, and interpretat¡on of data; in thewr¡t¡ng of the report; and ¡n the dec¡sion to submit the paper for pubtication. lf the study sponso(s) hadno such ¡nvolvement, the authors should so state.
Please state any sources of funding for your research
This work was supported by M¡n¡ster¡o de Educdc¡ón y C¡encid (AGL2OO6-127'O-CO2-OU02/ALDand cofinanced by FEDER funds. J.P. and J.V.M. were hired bv the CSIC, the former under apredoctoral /3P contract and the latter under a Ramón y Cajol postdoctoral contract, both of themfinanced by the Spanish M¡n¡sterio de Ciencía e lnnovación and the EuroDean Soc¡al Fund.All above mentioned funding bodies had no role ¡n the des¡gn, the contents, or the writ¡ng of thispaper, nor in the decision to submit th¡s paper for publication.
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Javier Para
Toxicology LettersConflict of Interest Policy
Synthes¡s of azoxystrob¡n transformat¡on products andselection of monoclonal antibodies for immunoassaydevelopment
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DeclarationsToxicology Leúfels requires that all authors sign a declarat¡on of conflicting interests. lf you havenoth¡ng to declare in any ofthese categories then this should be stated.
Conflict of Interest and Source of Fund¡ngA conf¡¡ct of ¡nterest exists when an author or the author's ¡nstitution has a financial or other relationshipw¡th other people or organizations that may ¡nappropriately influence the authois actions. Allsubmissions to Toxicology Leffers must include disclosure of all relat¡onships that could be viewed aspresent¡ng a potential confl¡ct of interest. Ioxicotogy Letfers may use such information as a basis fored¡tor¡al decisions and may publish such d¡sclosures if they are bel¡eved to be important to readers ¡njudging the article.
Conflict of Interest Statement for AuthorsAll authors must disclose any f¡nancial, personal, or their relationships with other people or organizationswith¡n 3 years of beginn¡ng the work submitted that could ¡nappropr¡ately ¡nffuence the work submiüed.Examples of conflicts include employment, consultancies, stock ownersh¡p, honorar¡a, pa¡d experttestimony, patent appl¡cations/registrat¡ons, and grants. lf there are no conflicls of interest, authorsshould state that there are none in the box below. Investigators should disclose potential confl¡cts toparticipants ¡n clinical tr¡als and other studies and should state ¡n the manuscr¡ot whether thev havedone so. Tox¡cology Leffers may decide not to publish on the bas¡s of a declared conflict, such as thefinancial interest of an author in a company (or its competitors) that makes a product discussed in thepaper.
' I i i : : -
Consuelo Agulló
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Role of Funding SourceAll sources of fund¡ng should be declared in the box below. Authors must also describe the role of thestudy sponsor(s), ¡f any, ¡n a study design; in the collection, analysis, and interpretation of data; in thewriting of the report; and in the decision to submit the paper for publicat¡on. tf the study sponso(s) hadno such involvement, the authors should so state.
Please state any sources of fund¡ng for ygur research
This work was suppoftedby M¡n¡ster¡o de Educac¡ón y Cienc¡d (AGL2OO6-12750-COZ-O1,/02/ALl\and cof¡nanced by FEDER funds. J.P. and J.V.M. were hired by the CSIC, the former under apredoctoral /3P contract and the latter unde¡ a Ramón y Cojdl postdoctoral contract, both of themfinanced by the Spanish M¡n¡ster¡o de C¡enc¡o e lnnovacíón and the European Social Fund.All above ment¡oned funding bodies had no role ¡n the des¡gn, the contents, or the wr¡t¡ng ofth¡spaper, nor in the decision to submit this paper for publication.
P¡int name
Consuelo Agulló
Toxicology LettersConflict of Interest Policy
Synthes¡s of azoxystrobin transformation products andselection of monoclonal antibod¡es for ¡mmunoassavdevelopment
DeclarationsToxicology Lefters requires that all authors sign a declarat¡on of conflicting interests. lf you havenothing to declare in any ofthese categories then this should be stated.
Conflict of Interest and Source of FundingA confl¡ct of ¡nterest ex¡sts when an author or the autho/s inst¡tution has a f¡nanc¡al or other relat¡onshipwith other people or organizations that may inappropriately ¡nfluence the author's act¡ons. Allsubmissions to Tox¡cology Letters must include d¡sclosure of all relationships that could be viewed aspresent¡ng a potential confl¡ct of inlerest. Tox¡cology Leffers may use such information as a bas¡s fored¡tor¡al decisions and may publ¡sh such disclosures if they are believed to be ¡mportant to readers injudg¡ng the art¡cle.
Conflict of Interest Statement for AuthorsAll authors must d¡sclose any f¡nanc¡al, personal, or their relationships w¡th other people or organizationswithin 3 years of beginning the work submitted that could inappropriately influence the work subm¡tted.Examples of conflicts ¡nclude employment, consultanc¡es, stock ownership, honoraria, pa¡d experttest¡mony, patent app¡icat¡ons/registrations, and grants. lf there are no confl¡cts of ¡nterest, authorsshould state that there are none in the box below. Invest¡gators should disclose potent¡al conflicts topartic¡pants ¡n clinical tr¡als and other studies and should state ¡n the manuscript whether they havedone so. Ioxrbology Leffers may decide not to publ¡sh on the bas¡s of a declared conflict, such as thef¡nancial interest of an author in a company (or ¡ts competitors) that makes a product discussed in thepaper.
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' : 1 r , . , . , .
Antonio Abad-Somovilla
The author declares that there are no conflicts of interest
Th¡s work was supported by Mín¡ster¡o de Educoción y C¡encra (AG12006-1275O-C02-01,/O2/ALl\and cofinanced by FEDER funds. J.P. and J.V.M. were hired by the CSIC, the former under apredoctoral /3P contract and the latter under a Romón y Cajol postdoctoral contract, both of themfinanced by the Spanish M¡n¡ster¡o de C¡encio e lnnovoc¡ón and the European Social Fund.All above mentioned funding bodies had no role ¡n the design, the contents, or the writ¡ng of thispaper, nor ¡n the decision to submit this paper for publication.
Print name
Antonio Abad-Somovilla
but each¡s acceptable,
. , . t . ; '
Toxicology LettersConfl¡ct of Interest Pol¡cy
. i . , r ' . i ;1 i , r rSynthesis of azoxystrobin transformation products andselection of monoclonal antibodies for immunoassaydevelopment
DeclarationsToxicology Leffefs requires that all authors s¡gn a declarat¡on of conflicting interests. lf you havenothing to declare in any of these categor¡es then this should be stated.
Confl¡ct of Interest and Source of FundingA conflicl of interest ex¡sts when an author or the author's institution has a financial or other relationshiDwith other people or organizations that may inappropriately influence the author,s act¡ons. Allsubm¡ss¡ons lo Tox¡cology Letters must include disclosure of all relationshiDs that could be viewed aspresenting a potential confl¡ct of interest. roxrboloqy Lefters may use such information as a basis foreditorial decisions and may publish such disclosures ¡f they are believed to be ¡mportant to readers injudging the article.
Conflict of Interest Statement for AuthorsAll authors must d¡sclose any f¡nanc¡al, personal, or their relationships with other people or organizat¡onsw¡thin 3 years of beginning the work submitted that could ¡nappropriately influence the work subm¡tted.Examples of conflicts ¡nclude employment, consultanc¡es, stock ownership, honoraria, paid experttest¡mony, patent appl¡cat¡ons/registrations, and grants. ¡f lhere are no conflicts of interest, authorsshould state that there are none in the box below. Investigators should disclose potential confl¡cts topart¡cipants ¡n clinical trials and other studies and should state in the manuscript whether they havedone so. Tox¡cology Leffers may dec¡de not to publ¡sh on the basis of a declared conflict, such as thefinancial interest of an aulhor in a company (or its competitors) that makes a product discussed ¡n theDaDer.
Role of Fund¡ng SourceAll sources of fund¡ng should be declared in the box below. Authors must also describe the role of thestudy sponso(s), ¡f any, ¡n a study des¡gn; in the collect¡on, analys¡s, and interpretation of data; in thewriting of the report; and ¡n the decis¡on to submit the paper for publication. lf the study sponsor(s) hadno such involvement, the authors should so state.
Please state any sources of funding for your research
This work was supported by M¡n¡ster¡o de Educoc¡ón y C¡enc¡a lAGLzoo6-I27SO-CO2-11./O2/ ALlland cofinanced by FEDER funds. J.P. and J.V.M. were hired by the CS|C, the former under apredoctoraf /3P contract and the latter under a Ramón y Cojal postdoctoral contract, both of themf¡nanced by the Spanish M¡níster¡o de Cienc¡d e lnnovqc¡ón and the European Social Fund.All above mentioned funding bodies had no role in the design, the contents, or the wr¡ting of thispaper, nor in the decision to subm¡t this paper for publication.
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Josep V. Mercader
Josep V. Mercader
The author declares that there are no conflicts of interest