SURFE2R 96SE. Pump up your transporter research. · automated processing of experimental data. Export of analyzed or raw data to other software tools, e.g. Genedata Screener®, allows

SURFE2R 96SE. Pump up your transporter research.

SURFE2R 96 SE

Throughput for transporters The SURFE2R 96SE is designed for activity measurement of electrogenic membrane transporters and pumps, which generate currents too low to resolve by patch clamp or which are not expressed in membranes accessible for patch clamp (e.g. bacterial, organelles). The technology enables label- and radioactivity-free real time measurements with high time resolution. The SURFE2R 96SE is a fully parallel 96 well based system, generating 96 data points in about 5 minutes. The highly specialized software tools SURFControl and DataControl and a set of optimized workflow modules enable the automatization of the experiment starting with the preparation of the measuring sensor to export and data analysis.

High flexibility The SURFE2R 96SE employs Solid Supported Membrane (SSM)-based electrophysiology, which enables characterization of substrates, inhibitors, modulators and cofactors. Possible targets range from electrogenic membrane transporters like symporters, exchangers or uniporters to ion pumps and ligand-gated ion channels. Membrane fragments derived from cells, organelles, and tissues or liposomes containing the purified target protein can serve as test sample. The SURFE2R 96SE provides a very flexible assay design. Single dose experiments, cumulative IC50 or EC50 determinations or more complex workflows with duplicates and control sequences can be designed by the user.

SURFE2R 96SE. Pump up your transporter research.

• Recording of 96 wells in parallel• Automatization of experimental workflows• Automated data analysis and export• ~10.000 data points per day!

• Real-time data, not single point read-out• High signal amplification for small transmembrane currents• High time resolution

• Transporters, pumps and channels • Native membranes or proteoliposomes• Applies established SSM-based electrophysiology

• Requires only 0.1 - 1 µg protein per well• Label-free electrical measurements• Integration into CyBio® Felix• Flexible experimental design

Data

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tInhibition of PepT1. IC50 determination of Lys[Z(NO2)]-Val with five concentrations, duration 15 minutes, CHO cell membra-ne preparation.

Substrate specifity of OCT2. Three different substrates are pro-vided in increasing concentrations cumulatively in each well, duration 30 minutes, CHO cell membrane preparation.

Experimental workflow: timeline

5 min

2 min

sensor coating

electrophysiological measurement

initializing instrument

chip loadingsensor quality control

centrifugationstart instrument and software

30 min10 min

15 min

chip unloadingdata analysis

2 min

parallel sensor preparation

measurements

25 plates per day

Automatic data analysis SURFE2R 96SE experiment results are analyzed with DataControl, a Nanion developed software allowing straightforward and fast evaluation of large data volumes – the basis for high throughput experiments. DataControl provides flexible tools for compound handling, peak and area determination, calculations and curve fitting. The use of templates enables fully automated processing of experimental data. Export of analyzed or raw data to other software tools, e.g. Genedata Screener®, allows individual data handling and processing.

SURFE2R 96SE. Pump up your transporter research.

Specifications.Liquid handling platform CyBio® FeliX (WHD: 88 x 51 x 67 cm), including wash station for pipettor

96-channel pipettor Volume: 96 x 250 µl, speed: 3 - 300 µl/s

Solution exchange Time resolution: 5 ms, solution positions: 9 x 96

Solution consumption Substrate: 10 - 50 µl, inhibitor: 80 - 340 µl (per well and measurement)

Nanion refillable reservoir Including 2 reservoirs, control unit, software integration

Labware kit Teflon labware, lipid reservoir, tip starter kit, ultrasonic processor, tool kit

SURFE2R 96SE module Shielded measuring chamber, control unit and amplifier

Sensor plates 96 well-based recording plates; gold-coated sensors with 3 mm diameter

Reference electrodes Electrode board with 96 gold-coated pins

96-channel amplifier Gain: 4.85 x 108 V/A, resolution: 16 bit (± 5 V, 152 µV steps)

RMS noise: 0.4 pA, low pass: 250 Hz, rise time: 1.4 ms

Work station Computer with preinstalled software, 24” TFT monitor

Software package SurfControl + DataControl; for automated recording and data analysis

The automated sensor preparation can be performed independently from the measurements by using other automated liquid handling platforms.

Each measurement takes about 5 min. One typical measurement sequence contains three measure-ments: (1) negative control, (2) full activation and (3) inhibition. 3 x 96 datasets therefore take 15 min.

In this scenario 25 plates can be measured per day (8 h) yielding 25 x 3 x 96 = 7200 datasets.

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Interface of DataControl Software. The 96 datasets can be analyzed within minutes including statistical calculations, a pdf report and the option to export the data.

SURFE2R – measure more membrane

EuropeNanion Technologies Gabrielenstr. 9 80636 Munich Germany Phone: +49 89 218997972 Fax: +49 89 218997960 [email protected] www.nanion.de

TurkeyE-A Teknoloji LTD.ŞTİ. Cyberpark, Cyberplaza A Blok, Zemin Kat, no: AZ-05 Bilkent 06800 Ankara Turkeyphone: +90 312 265 09 [email protected]://www.eateknoloji.com

USANanion Technologies Inc.1 Naylon PlaceLivingston, NJ 07039USAPhone: +1 1-888-9-NANION [email protected]

RussiaBiotechnologies Ltd.Thorez pr. 44194223 St. PetersburgRussiaPhone: +7 812 2942206Fax: +7 911 [email protected]

India Medi Analytika India Pvt. Ltd No. 15 Adyar Bridge road Adyar, Chennai - 600 020 India Phone: +91 44 24460988 Fax: +91 44 24463931 [email protected] www.medianalytika.com

SingaporeBronjo Medi20 Woodlands Link #03-18Singapore 738 733SingaporePhone: +65 6 4764011Fax: +65 6 [email protected]

JapanNanion Technologies JapanMIL5, TWIns 3F, 2-2 Wakamatsu-choShinjyuku-ku, Tokyo 162-0056,Japan phone: +81 3 6457 8773 fax: +81 3 6457 8776 [email protected] www.nanion.de

China/TaiwanNanion Technologies ChinaRoom 337, Building 6, Yard No.110th Shangdi roadHaidian District, Beijing, 100085 ChinaPhone: +86 10821 76388Fax: +86 10821 [email protected]

Republic of KoreaRepublic of KoreaQuantum Design KoreaHana Bldg., 4F, 414-15, Dogok-dong,Kangnam-gu, Seoul, Korea 135-854Phone: +82 2 2057 2710Fax: +82 2 2057 [email protected]

“The SURFE2R makes extremely challenging electrophysiological targets accessible to robust routine analyses. For example, we have developed an assay for investigating nicotinic acetylcholine receptor a7 ion channels, using membranes from the Pacific electric ray eel, Torpedo California. In this way, we overcome many of the well-known difficulties associa-ted with this ion channel, which allows us to efficiently obtain information regarding com-pound pharmacology. Obtained pharmacology values match those of patch clamp re-cordings exceptionally well. We find that the SURFE2R is an excellent platform for characterization of electrogenic processes in isolated membranes.”

Karin V. Niessen, Dr. Thomas Seeger, Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany

“SSM-based electrophysiology is a well-established technique developed in our lab in the early 1990s for the investigation of electrogenic membrane transporters. It works with a wide range of samples from mammalian cell membranes over microbial membrane ve-sicles to proteoliposomes. SSM-based electrophysiology is especially useful in cases whe-re conventional electrophysiology cannot be applied, e.g. for transporters residing in intra-cellular membranes or plasma membranes of small microbial cells. The SURFE2R N1 is a commercial instrument for SSM based electrophysiology which facilitates basic research in the field of membrane transport.“

Prof. Dr. Klaus FendlerMax-Planck Institute of Biophysics, Frankfurt, Germany